Article
Biochemistry & Molecular Biology
Xiao-Qian Ma, Yu-Yin Liu, Zhi-Qi Zhong, Si-Man Chen, Wen-Ting Hu, Yan-Ran Sheng, Yu-Kai Liu, Chun-Yan Wei, Ming-Qing Li, Xiao-Yong Zhu
Summary: Endometriosis is an estrogen-dependent, progesterone-resistant gynecological disease. In this study, the dysregulated expression of heme-trafficking proteins and heme-degrading enzymes was investigated, revealing that heme mediates progesterone resistance and induces endometriosis via the mitochondrial oxidative phosphorylation pathway.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Hui-Chen Wang, Yen-Nien Huo, Wen-Sen Lee
Summary: We investigated the requirement of progesterone receptor (PR) activation for folic acid (FA)-regulated cell proliferation and migration in various cancer cell lines. Our results showed that FA only affected proliferation and migration in PR positive (+) cancer cell lines, and these effects were abolished by the PR inhibitor Org 31710. Transfecting PR pcDNA into PR-negative (-) cancer cell lines restored the effects of FA, while PR-transfected Hep-3B cell line lacking endogenous PR and FA receptor (FR) did not respond to FA. Immunoprecipitation assay demonstrated that PR formed a complex with FR and cSrc, suggesting that PR activation is a general phenomenon for FA-regulated cell proliferation and migration in all cancer cell lines.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Iva Busic-Pavlek, Ivo Dumic-Cule, Lucija Kovacevic, Milan Milosevic, Petra Delimar, Lea Korsa, Zlatko Marusic, Maja Prutki
Summary: The calcium-sensing receptor (CaSR) is important in maintaining calcium balance and altered signaling through CaSR is associated with the development of various tumors including colorectal and breast tumors. A retrospective study involving 79 patients with invasive breast carcinoma explored the expression of CaSR in breast cancer. The study found that higher CaSR expression was correlated with larger tumor size, presence of HER2 receptors, lymph node involvement, distant metastasis, and increased Ki-67 proliferation index. This suggests that higher CaSR expression may indicate a poor prognosis and treatment outcome in breast cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Ping Zhang, Guoyun Wang
Summary: Endometriosis is a disease where endometrial-like tissue grows outside the uterus. Progestins are the most commonly used treatment, but some patients show resistance to these drugs. The mechanisms of this progesterone resistance include abnormal PGR signaling, chronic inflammation, aberrant gene expression, epigenetic alterations, and environmental toxins. Understanding these mechanisms may help develop new therapeutic regimens to reverse progesterone resistance in endometriosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Endocrinology & Metabolism
Adali Pecci, Maria Florencia Ogara, Rosario T. Sanz, Guillermo Pablo Vicent
Summary: Steroid hormone receptors have unique characteristics, including simultaneous activation by multiple natural ligands and shared structural and functional properties. However, the specificity of response to a given receptor remains unclear and may be influenced by factors such as receptor expression levels, interaction with co-regulators, chromatin accessibility, and DNA sequence of target regions.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Medicine, General & Internal
Iwona Szydlowska, Marta Grabowska, Jolanta Nawrocka-Rutkowska, Malgorzata Piasecka, Andrzej Starczewski
Summary: The results of the study suggest that a good response to UPA treatment in fibroids may be associated with a decrease in fibrosis, ER/PR and PCNA and Ki67 immunoexpression, as well as an increase in cell apoptosis.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Ping Xu, Zhiheng Liu, Ying Liu, Huazheng Ma, Yiyuan Xu, Ying Bao, Shiyou Zhu, Zhongzheng Cao, Zeguang Wu, Zhuo Zhou, Wensheng Wei
Summary: BARBEKO is a DSB-independent, genome-wide CRISPR screening method that leverages CRISPR cytosine base editors to perturb gene start codons or splice sites for KO screening. By integrating with the iBAR strategy, BARBEKO improves screening quality and efficiency, yielding efficient and accurate results with reduced starting cells. Moreover, BARBEKO screens are not impacted by DNA cleavage-induced cytotoxicity in specific cell types, offering a valuable tool to complement current CRISPR-KO screens.
NATURE BIOTECHNOLOGY
(2021)
Article
Cell Biology
Maria Victoria Zanardi, Maria Paula Gastiazoro, Georg Kretzschmar, Jannette Wober, Guenter Vollmer, Jorgelina Varayoud, Milena Durando, Oliver Zierau
Summary: Hops extract, particularly the compound 6-prenylnaringenin, has estrogenic activity and can activate both estrogen receptor and aryl hydrocarbon receptor pathways. It has the potential to modulate estrogen metabolism and increase the expression of tumor suppressor genes and genes involved in estrogen metabolism.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Yassmin Medina-Laver, Cristina Rodriguez-Varela, Stefania Salsano, Elena Labarta, Francisco Dominguez
Summary: Progesterone hormone acts through classical and non-classical receptors in the female reproductive system, affecting menstrual cycle, pregnancy, and parturition. These receptors regulate cell responses through different signaling pathways, playing a crucial role in female reproductive tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Rong Li, Tianyuan Wang, Ryan M. Marquardt, John P. Lydon, San-Pin Wu, Francesco J. DeMayo
Summary: Estrogen and progesterone regulate uterine biology through their receptors ERα and PR, respectively. This study identifies TRIM28 as a protein that interacts with ERα and PR to regulate uterine function. TRIM28 deficiency disrupts PR and ERα chromatin binding, resulting in the inability of the uterus to support early pregnancy.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Warren B. Nothnick
Summary: Endometriosis is a significant disease characterized by infertility and pelvic pain. Altered responsiveness to progesterone, potentially regulated by miRNAs, may contribute to the pathophysiology of the disease.
Article
Oncology
Cornelia Liedtke, Chafika Mazouni, Kenneth R. Hess, Fabrice Andre, Attila Tordai, Jaime A. Mejia, W. Fraser Symmans, Ana M. Gonzalez-Angulo, Bryan Hennessy, Marjorie Green, Massimo Cristofanilli, Gabriel N. Hortobagyi, Lajos Pusztai
Summary: This study compared the response to neoadjuvant chemotherapy and survival between patients with triple-negative breast cancer (TNBC) and non-TNBC. The results showed that TNBC patients had higher pathologic complete response rates but lower 3-year progression-free survival rates and 3-year overall survival rates. TNBC was associated with increased risk for visceral metastases, lower risk for bone recurrence, and shorter postrecurrence survival. Patients with TNBC had worse survival if they had residual disease after neoadjuvant chemotherapy.
JOURNAL OF CLINICAL ONCOLOGY
(2023)
Review
Oncology
Juberiya M. Azeez, Thachapillil Ramakrishnan Susmi, Viji Remadevi, Vini Ravindran, Anjana Sasikumar Sujatha, Raveendran Nair Sobha Ayswarya, Sreeharshan Sreeja
Summary: Progesterone, an ovarian steroid hormone, plays a crucial role in regulating biological processes in various tissues. Understanding its role in diseases, particularly breast cancer, is a topic of debate due to conflicting evidence. Further research is needed to explore how progesterone affects breast cancer progression and its impact on different molecular pathways.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Michael E. Baker
Summary: This article discusses the evolution of steroid receptors in ray-finned fish and terrestrial vertebrates, as well as the different responses to various steroids in these species. The progesterone receptor and mineralocorticoid receptor in fish and terrestrial vertebrates have evolved to respond differently to different steroids. These differences should be considered when studying the disruption of steroid hormones by chemicals in these species.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Oncology
Teeranut Asavasupreechar, Ryoko Saito-Koyama, Yasuhiro Miki, Keiichi Tamai, Jiro Abe, Chihiro Inoue, Ikuro Sato, Viroj Boonyaratanakornkit, Hironobu Sasano
Summary: This study revealed that the presence of progesterone receptor (PR) and PR isoform B (PRB) is correlated with better clinical outcomes in breast cancer and non-small cell lung cancer (NSCLC). The microRNA let-7b can inhibit cell proliferation by inducing PR and PRB expression in breast cancer cell lines. The let-7b-E2F1 interaction was found to be responsible for the inhibition of PRB in breast cancer cells.
ENDOCRINE-RELATED CANCER
(2023)