Article
Biotechnology & Applied Microbiology
Qin Yan, Laichuang Han, Xinyue Liu, Cuiping You, Shengmin Zhou, Zhemin Zhou
Summary: An efficient auto-inducible protein expression system based on nitrogen catabolite regulation was constructed in this study. The system successfully expressed glycosylated and secretory proteins, providing a new option for protein production in laboratory and industrial applications.
MICROBIAL CELL FACTORIES
(2022)
Article
Microbiology
Lana O'Hara Souza Silva, Thalison Rodrigues Moreira, Relber Aguiar Goncales, Mariana Vieira Tomazett, Juliana Alves Parente-Rocha, Karine Mattos, Juliano Domiraci Paccez, Orville Hernandez Ruiz, Maristela Pereira, Celia Maria de Almeida Soares, Simone Schneider Weber, Vanessa Rafaela Milhomem Cruz-Leite, Clayton Luiz Borges
Summary: Nitrogen is crucial for microorganisms, but hosts limit this nutrient to counter infections, thereby pathogens use adaptive mechanisms. In fungi, nitrogen catabolite repression (NCR) activates transcription factors to acquire nitrogen from alternative sources. This study investigates the importance of formamidase in Paracoccidioides lutzii and finds that formamidase plays a role in nitrogen assimilation and fungal virulence.
Article
Biochemistry & Molecular Biology
Lin Zeng, Robert A. Burne
Summary: The study revealed that streptococcus exhibits bet-hedging behaviors in carbohydrate metabolism, which may significantly influence the composition and pathogenic potential of oral biofilms.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Abhinav Nair, Saurabh Jyoti Sarma
Summary: Organisms use catabolite repression to adjust resource utilization to gain a competitive edge in unfavorable conditions, playing a crucial role in microbial survival. Studying genes and proteins linked to preferential uptake mechanisms can identify regulatory processes that promote microbial growth and survival.
MICROBIOLOGICAL RESEARCH
(2021)
Review
Biotechnology & Applied Microbiology
Joshua D. D. Kerkaert, Lori B. B. Huberman
Summary: Organisms need to accurately sense and respond to nutrients for survival. Fungi have evolved genetic networks to regulate metabolic pathways and distinguish between preferred and nonpreferred nutrients. Transcription factors and regulatory genes play important roles in this regulation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Yingying Chen, Liguo Dong, Md Ashiqul Alam, Lakhansing Pardeshi, Zhengqiang Miao, Fang Wang, Kaeling Tan, Michael J. Hynes, Joan M. Kelly, Koon Ho Wong
Summary: This study investigated the regulatory role of the carbon catabolite repression (CCR) transcription factor CreA in Aspergillus nidulans, uncovering its functions in diverse physiological processes beyond carbon metabolism. The research provided mechanistic insights into CreA regulation and highlighted its role as a master regulator controlling multiple transcription factors in different regulatory networks. The discoveries have important implications for understanding CCR in related pathogenic and industrial species.
Article
Biotechnology & Applied Microbiology
Aroa Ferrer-Pinos, Victor Garrigos, Emilia Matallana, Agustin Aranda
Summary: Wine strains of Saccharomyces cerevisiae need to adapt their metabolism to different growth conditions during biotechnological applications, with the transcription factor Gln3 playing a crucial role in glucose and nitrogen repression. Commercial yeast strains exhibit a variety of glucose repression profiles, and deletion of the GLN3 gene results in different mutant phenotypes. The nitrogen catabolite repression controlled by TORC1 is largely conserved in winemaking conditions, with mutants showing increased tolerance to rapamycin inhibition of nutrient signaling complex TORC1.
FERMENTATION-BASEL
(2021)
Article
Multidisciplinary Sciences
Chunghwan Ro, Michael Cashel, Llorenc Fernandez-Coll
Summary: This study investigates the regulatory mechanisms of cAMP-CRP system on the lac operon and reveals that ppGpp can modulate the expression of lacZ by regulating the acetylation of CRP, thus impacting the growth status of cells in glycerol-containing minimal media.
Article
Ecology
Tao Xu, Xuanyu Tao, Hongxi He, Megan L. Kempher, Siping Zhang, Xiaochun Liu, Jun Wang, Dongyu Wang, Daliang Ning, Chongle Pan, Honghua Ge, Nannan Zhang, Yong-Xing He, Jizhong Zhou
Summary: Carbohydrate utilization is critical for microbial survival. The phosphotransferase system (PTS) is a well-known microbial system involved in carbohydrate metabolism, but its role in non-model prokaryotes remains underexplored. In this study, the prevalence of incomplete PTSs in prokaryotes was investigated, and lignocellulose-degrading clostridia was found to have lost PTS sugar transporters and carry a substitution in the core PTS component. Functional and structural diversification of PTS components were observed, providing novel insights into the regulatory mechanisms of incomplete PTSs.
Article
Biochemistry & Molecular Biology
Xin-Xin Kang, Qin-Qing Wang, Zhe Chi, Guang-Lei Liu, Zhong Hu, Zhen-Ming Chi
Summary: The study showed that by removing GATA-type transcriptional activator and repressor genes, as well as overexpressing mutated genes with specific residues, the pullulan production of Aureobasidium melanogenum P16 could be significantly increased.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Naran Naren, Xue-Xian Zhang
Summary: The study reveals a unique negative feedback mechanism that allows the global NtrB/NtrC regulator to rapidly respond to nitrogen starvation in Pseudomonas fluorescens. HutC and NtrC bind to the same operator site in the ntrBC promoter, fine-tuning the autoactivation of ntrBC to prevent overexpression. This interaction plays a crucial role in governing NtrBC to maintain carbon/nitrogen homeostasis through the complex interactions between two TCSs (NtrBC and CbrAB) at the hut promoter.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Biotechnology & Applied Microbiology
Anu Jose Mattam, Yogesh Babasaheb Chaudhari, Harshad Ravindra Velankar
Summary: The growing demand for biofuels has led to the search for alternative feedstock, and lignocellulosic biomass is a promising candidate. The conversion of lignocellulosic biomass to bioethanol involves a complex process with multiple unit operations. The expression of cellulases, which are necessary for saccharification, is controlled by transcription factors and sugar transporters, and genetic engineering efforts have been made to modulate their expression.
MICROBIAL CELL FACTORIES
(2022)
Article
Microbiology
Linda Paetzold, Anne-Christine Brausch, Evelyn-Laura Bielefeld, Lisa Zimmer, Greg A. Somerville, Markus Bischoff, Rosmarie Gaupp
Summary: The carbon catabolite repression (CCR) is a mechanism used by pathogenic bacteria to link central metabolism with virulence factor synthesis. In Staphylococcus aureus, the regulatory functions of CcpA are well known, while the function of HPr in CCR and infectivity remains unclear. Mutations in ptsH altered bacterial physiology and decreased the ability of S. aureus to form a biofilm and cause infections in mice, showing that HPr affects central metabolism and virulence in S. aureus independently of CcpA regulation.
Article
Microbiology
Stephane Duboux, Solenn Pruvost, Christopher Joyce, Biljana Bogicevic, Jeroen Andre Muller, Annick Mercenier, Michiel Kleerebezem
Summary: Bifidobacteria can metabolize a variety of carbohydrates through their unique central carbon metabolism known as the bifid-shunt. The growth rate, transcriptional changes, and central carbon fluxes of Bifidobacterium longum NCC 2705 are affected by the carbon source used for growth, particularly glucose and galactose. Galactose-containing oligosaccharides in the human infant gut can influence the physiological characteristics of B. longum NCC 2705, such as cell size, sugar metabolism, and acid-stress resistance.
Article
Multidisciplinary Sciences
Fanyang Lv, Yuhua Zhan, Wei Lu, Xiubin Ke, Yahui Shao, Yiyuan Ma, Juan Zheng, Zhimin Yang, Shanshan Jiang, Liguo Shang, Yao Ma, Lei Cheng, Claudine Elmerich, Yongliang Yan, Min Lin
Summary: Research shows that Pseudomonas stutzeri A1501 preferentially metabolizes succinate, followed by citrate and ultimately glucose. The Hfq/Crc/CrcZY regulatory system orchestrates this preference and contributes to optimal nitrogenase activity and efficient root colonization.
Article
Microbiology
Maise Gomes Queiroz, Carolina Elsztein, Marcos Antonio De Morais
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2020)
Article
Microbiology
Allyson Andrade Mendonca, Paula Katharina Nogueira da Silva, Tiago Luiz Santana Calazans, Rafael Barros de Souza, Carolina Elsztein, Marcos Antonio de Morais Junior
MICROBIOLOGICAL RESEARCH
(2020)
Article
Energy & Fuels
Barbara Ribeiro Alves Alencar, Nilson Medeiros, Carolaine Larissa Lira da Silva, Aldo Torres, Emmanuel Damilano Dutra, Everardo Valadares de Sa Barreto Sampaio, Romulo Simoes Cezar Menezes, Marcos Antonio Morais Junior
Summary: This study evaluated the effects of harvest time and season on biomass pH and dry matter concentration of cactus pear cladodes and compared the hydrolysis and fermentation of fresh and dried biomass. The results showed that drying at 105 degrees C for 12 hours yielded the best results in terms of solids concentration. The enzymatic hydrolysis and fermentation of the dried biomass resulted in the production of glucose and ethanol.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biotechnology & Applied Microbiology
Irina Charlot Pena-Moreno, Denise Castro Parente, Karolini Miranda da Silva, Elton Pedro Nunes Pena, Fabiana Aparecida Cavalcante Silva, Tercilio Calsa Junior, Will de Barros Pita, Marcos Antonio de Morais Jr
Summary: The yeast Dekkera bruxellensis exhibits superior anaerobic growth and ethanol yield in the presence of nitrate, due to balanced activation of TORC1 and NCR de-repression mechanisms regulating cell metabolism. On the other hand, poor growth in aerobiosis is likely caused by oxidative stress triggered by nitrate when oxygen is present.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Maise Gomes Queiroz, Carolina Elsztein, Sabine Strahl, Marcos Antonio de Morais Junior
Summary: The Ncw2 protein encoded by the NCW2 gene plays a crucial role in remodeling the cell wall structure in yeast, facilitating the correct chitin/β-glucan structuring and increasing chitin deposition for glucanase resistance. This protein is transported to the cell surface through O-mannosylation, interacting with yeast bud scars and aiding in cell surface rigidity control.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Manuella Maria Silva Santos, Damian Gatica, Jaqueline de Azevedo Silva, Sergio Crovella, Daniel J. Klionsky, Marcos Antonio De Morais Jr
Summary: Limitation of mevalonate kinase activity leads to dysfunctional mitochondria that may not be recycled, causing metabolic dysfunctions in the cells. Understanding this mechanism may help in solving the nonspecific auto-inflammatory response puzzle observed in MKD patients.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Biotechnology & Applied Microbiology
Gilberto Henrique Teles, Jackeline Maria Da Silva, Mariana Rodrigues Xavier, Rafael Barros De Souza, Will de Barros Pita, Marcos Antonio De Morais Junior
Summary: The physiology of Dekkera bruxellensis has great potential for the production of fuel ethanol. This study evaluated the respiro-fermentative parameters of D. bruxellensis under different cultivation conditions. The results showed that sucrose was more efficiently converted to ethanol than glucose, and blocking acetate production increased fermentative efficiency. The presence of nitrate reduced ethanol production. Acetate metabolism plays a key role in ethanol production and carbon flow regulation in this yeast.
JOURNAL OF BIOTECHNOLOGY
(2022)
Review
Microbiology
Allyson Andrade Mendonca, Walter de Paula Pinto-Neto, Giselle Alves da Paixao, Dayane da Silva Santos, Marcos Antonio De Morais Jr, Rafael Barros De Souza
Summary: This review provides an overview of the technological and biological challenges in the production and use of probiotic bacteria for promoting human health. It discusses the challenges in industrial production, from physiology to stress resistance, and emphasizes the importance of maintaining cell viability and vitality in the product. It also highlights the obstacles probiotic cells face within the human body, such as enzymes, antibacterial molecules, and pH changes.
Article
Biochemistry & Molecular Biology
Gilberto Henrique Teles, Mariana Rodrigues Xavier, Jackeline Maria Da Silva, Rafael Barros De Souza, Will de Barros Pita, Marcos Antonio de Morais Jr
Summary: Dekkera bruxellensis is an important yeast in industrial fermentation processes. Acetate, a metabolite found in its cultivations, affects its fermentation capacity. This study investigated the role of acetate metabolism in respiring cells using different nitrogen sources. The results showed that acetate production is crucial for carbon assimilation and provided valuable insights into the oxidative metabolism of this yeast.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Barbara Ribeiro Alves Alencar, Renan Anderson Alves de Freitas, Victor Emanuel Petricio Guimaraes, Rayssa Karla Silva, Carolina Elsztein, Suzyanne Porfirio da Silva, Emmanuel Damilano Dutra, Marcos Antonio de Morais Junior, Rafael Barros de Souza
Summary: In this study, a yeast strain called Meyerozyma caribbica URM 8365 was isolated and tested from soil irrigated with vinasse, which has high mineral content and acidic pH. The strain was able to ferment glucose and use xylose when oxygen was provided. It also showed tolerance to inhibitors and high ethanol production efficiencies in various substrates. A proposed metabolic model allowed for the calculation of carbon allocation for ethanol and/or xylitol production and could be used to design an industrial plant for combined production using different substrates.
Article
Biotechnology & Applied Microbiology
Rayssa Karla Silva, Will de Barros Pita, Marcos Antonio de Morais, Rafael Barros de Souza
Summary: This study investigated the detrimental effect of excessive minerals in industrial substrates on the fermentation performance of Saccharomyces cerevisiae and its effect on the physiology of Dekkera bruxellensis. The highest mineral toxicity was observed with Cu2+, which depended on the level of medium aeration. Copper stimulated respiration and glucose fermentation was hindered by most growth inhibitors, leading to changes in carbon distribution. The negative effect of Cu2+ on yeast fermentation was partially alleviated by Mg2+ and Mn2+, similar to magnesium antagonism observed in S. cerevisiae.
LETTERS IN APPLIED MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Mariana Rodrigues Xavier, Gilberto Henrique Teles, Jennyfer Martins de Carvalho, Leucio Duarte Vieira, Will de Barros Pita, Marcos Antonio de Morais
Summary: This study aimed to investigate the biological defence mechanism used by the yeast Dekkera bruxellensis to tolerate the presence of exogenous H2O2. The combination of glutathione peroxidase and sulfhydryl-containing proteins was found to be the preferred defence mechanism against H2O2, especially under respiratory metabolism. However, the action of this mechanism was suppressed when the cells were metabolizing nitrate.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)