Article
Agronomy
Yijun Huang, Liangliang Zhang, Shijia Yuan, Wenpei Liu, Chaochun Zhang, Da Tian, Xinxin Ye
Summary: In this study, the production of organic acid by Aspergillus niger (A. niger) under different lead (Pb) concentrations was investigated. A. niger showed high Pb tolerance and was able to produce oxalic acid even at 1500 mg/L Pb concentration. The formation of lead oxalate minerals played a key role in protecting A. niger from Pb toxicity. Moreover, the presence of Pb stimulated the activity of pyruvate dehydrogenase enzyme and improved the production of oxalic acid by A. niger.
Article
Biotechnology & Applied Microbiology
Xia Kang, Laszlo Csetenyi, Xiang Gao, Geoffrey Michael Gadd
Summary: In this research, the solubilization of natural struvite by Aspergillus niger and its ability to recover cerium were investigated. The study found that after two weeks of fungal growth, struvite was completely solubilized and released inorganic phosphate through the production of oxalic acid. Various analyses confirmed the formation of cerium minerals, which led to the removal of over 99% of cerium from the solution. Thermal decomposition experiments showed that the biogenic cerium phosphates could be transformed into a mixture of cerium phosphate and cerianite after heat treatment. These findings highlight the potential of using struvite leachate for the biotransformation of soluble rare earth elements and the recovery of cerium for various applications.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Liyan Wang, Hao Guan, Jun Hu, Yi Feng, Xiang Li, Kianpoor Kalkhajeh Yusef, Hongjian Gao, Da Tian
Summary: This study investigated the mechanisms of wheat straw degradation and phosphorus release by the phosphate-solubilizing fungus Aspergillus niger. The results showed that A. niger significantly increased wheat straw degradation and released more phosphorus compared to the control group. The high enzyme activity and oxalic acid secretion by A. niger were identified as the main factors contributing to wheat straw degradation and phosphorus release.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Microbiology
Thanaporn Laothanachareon, Lyon Bruinsma, Bart Nijsse, Tom Schonewille, Maria Suarez-Diez, Juan Antonio Tamayo-Ramos, Vitor A. P. Martins dos Santos, Peter J. Schaap
Summary: The study found that blocking active citrate export in Aspergillus niger does not affect citrate uptake, resulting in the production of a large amount of oxalate. In addition, blocking citrate export reduces glucose uptake and the expression of high-affinity glucose transporter genes.
Article
Food Science & Technology
Arzu Cagri Mehmetoglu, Elif Sezer, Sumeyye Erol
Summary: The study confirmed that silver nanoparticles synthesised by Aspergillus niger have significant antimicrobial and mechanical properties enhancing effects on whey protein concentrate (WPC) based films, while also improving the tensile strength and water barrier properties of the films.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Ruben Halfwerk, Doekle Yntema, Jaap Van Spronsen, Albert Van der Padt
Summary: This study investigates a new separation technique operating at sub-zero conditions, which enables the crystallization and recovery of lactose from delactosed whey permeate. The findings show the potential for efficient lactose recovery from this by-product, providing a valuable solution for its utilization.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Food Science & Technology
Abhay Sharma, Sandipan Mukherjee, Subbi Rami Reddy Tadi, Aiyagari Ramesh, Senthilkumar Sivaprakasam
Summary: This study validated the potential of using whey permeate and whey protein hydrolysate as alternate carbon and nitrogen sources for enhanced production of plantaricin and lactic acid. The optimal WP and WPH concentration ratio of 2:1 was found for the production of PL and LA. Kinetic modeling revealed that Aiba substrate inhibition model exhibited the best fit for the experimental data.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Liu Wu, Licheng Zhang, Xiaojie Li, Ruitong Lv, Wei Cao, Weixia Gao, Jiao Liu, Zhoujie Xie, Hao Liu
Summary: In this study, the heterologous production of kojic acid (KA) in Aspergillus niger was achieved through the expression of the kojA gene from Aspergillus oryzae. The study also identified four genes (nrkA, nrkB, nrkC, and nrkD) that negatively regulate KA production, and showed that knocking down nrkA or deleting any of the other three genes can significantly increase KA production. The highest KA titer of 25.71 g/L was achieved in a pH controlled batch bioreactor using the kojA overexpression strain with a deletion of nrkC, which showed a 26.7% improvement compared to shaking flask cultivation.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Y. Aso, Y. Nomura, M. Sano, R. Sato, T. Tanaka, H. Ohara, K. Matsumoto, K. Wada
Summary: The study aimed to enhance the production of alkylitaconic acids by Aspergillus niger S17-5 through the addition of caprylic acid as a precursor. Results showed that addition of caprylic acid improved the production efficiency of 9-HHIA and 10-HHIA, especially when added in the mid-growth phase.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xiaomei Zheng, Peng Du, Kaiyue Gao, Yimou Du, Timothy C. Cairns, Xiaomeng Ni, Meiling Chen, Wei Zhao, Xinrong Ma, Hongjiang Yang, Ping Zheng, Jibin Sun
Summary: This study analyzed the genome-wide transcriptional changes of Aspergillus niger in response to external glucose gradient, revealing the important regulatory role of glucose signaling in cellular processes. It provides valuable insights into the adaptive evolutionary mechanism of citric acid production in A. niger.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Applied
Paraskevi Tsermoula, Mie Rostved Bechsh, Christoffer Friis, Soren Balling Engelsen, Bekzod Khakimov
Summary: NPN compounds from dairy processing side-streams show promise as a source for new products. The NPN profile of lactose production samples was analyzed using GC-MS and 1H NMR spectroscopy, identifying 35 NPN compounds including amino acids, organic acids, and other classes. The quantification of NPN compounds revealed their decrease during lactose production, with urea, ammonia, glycerophosphocholine, creatine, creatinine, orotic acid, and choline being the most dominant. Mother liquor concentrate had the highest NPN concentration, while lactose powder had substantial amounts of N-acetylglucosamine, phosphocholine, and orotic acid. The NPN compounds accounted for 57-99% of total nitrogen, with the highest recovery found in reverse osmosis retentate, mother liquor concentrate, wash water, and reverse osmosis permeate." The lowest recovery was found in lactose powder.
Article
Engineering, Environmental
Junman Zhang, Ruixia Hao, Bing Shan, Yubo Ye, Jiani Li, Anhuai Lu
Summary: This study investigates the biomineralization of lead ions by Aspergillus niger from aqueous environments, focusing on the dynamic effects of fungal metabolism and biological components. It reveals that A. niger plays a crucial role in controlling the mineralization process of Pb (II), with biomineralization experiments demonstrating the specific morphogenesis of lead oxalate over time. The inclusion of the three biomolecules in the system indirectly influenced the rate of Pb (II) removal and mineral morphology, suggesting the potential application of A. niger-mediated biomineralization in the removal of Pb (II) from aqueous environments.
WATER ENVIRONMENT RESEARCH
(2023)
Article
Microbiology
Wei Cao, Licheng Zhang, Liu Wu, Mingyi Zhang, Jiao Liu, Zhoujie Xie, Hao Liu
Summary: "This study identified and characterized the mitochondrial citrate transporters (Ctps) in Aspergillus Niger, a major cell factory for citric acid production. The results showed that CtpA, CtpB, and CtpD play important roles in citric acid transport across the mitochondrial membrane and function redundantly. Enhancing the citric acid transport process could serve as a target for boosting citric acid accumulation in A. niger."
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Jianrong Zhai, Bo Wang, Yingpeng Sun, Jianfeng Yang, Junfeng Zhou, Tianyu Wang, Wenlan Zhang, Cai Qi, Yanjun Guo
Summary: Cyanogenic glycosides (CNglcs) in fresh ratooning sorghum exceeded the safety threshold and could not be reduced by silage fermentation. However, the addition of A. niger degraded CNglcs and removed hydrogen cyanide (HCN), reducing the content to an acceptable level.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Francielle Herrmann Mobayed, Juliane Carraro Nunes, Adriano Gennari, Bruna Coelho de Andrade, Matheus Loch Velvites Ferreira, Paolla Pauli, Gaby Renard, Jocelei Maria Chies, Giandra Volpato, Claucia Fernanda Volken de Souza
Summary: The study evaluated the efficiency of lactose derived from cheese whey and cheese whey permeate as inducer of recombinant Kluyveromyces sp. beta-galactosidase enzyme produced in Escherichia coli. The results showed that induction with cheese whey permeate was more efficient for recombinant beta-galactosidase expression than other inducers, representing a cost-effective alternative in recombinant protein production.
BIOTECHNOLOGY LETTERS
(2021)