Article
Microbiology
Chang Liu, Xiuyan Ding, Long Chang, Weifeng Yuan, Shitao Li, Liqian Zhu
Summary: This study found that PLC-gamma 1 interacts with beta-catenin, and the PLC-gamma 1 signaling pathway potentially regulates beta-catenin signaling stimulated by BoHV-1 infection by affecting the subcellular localization of p-beta-catenin(S552).
VETERINARY MICROBIOLOGY
(2023)
Article
Virology
Fouad S. El-Mayet, Gabriela Toomer, Jeffery B. Ostler, Kelly S. Harrison, Vanessa Claire Santos, Nishani Wijesekera, Erin Stayton, Jerry Ritchey, Clinton Jones
Summary: Steroid hormone fluctuations are predicted to enhance or initiate bovine herpesvirus 1 (BoHV-1) replication and virus spread in cattle. Progesterone stimulates BoHV-1 productive infection and sporadically induces reactivation from latency, while heat stress augments this reactivation.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Francesca Oberto, Emanuele Carella, Claudio Caruso, Pier Luigi Acutis, Davide Lelli, Luigi Bertolotti, Loretta Masoero, Simone Peletto
Summary: The aim of this study was to develop a qualitative PCR assay that allows the discrimination of BuHV-1, BoHV-1 and BoHV-5. Specific nucleotide sequences of BuHV-1, BoHV-1 and BoHV-5 were identified through the alignment of homologous genes. The design of the primers and the optimization of the PCR assay were focused on the target sequences located on the portions of gD, gE and gG genes. This qualitative PCR assay allowed the differentiation of BoHV-1 and BoHV-5 infections in both cattle and water buffaloes, as well as heterologous BuHV-1 infections in bovine.
Article
Biochemistry & Molecular Biology
Natalia Karska, Malgorzata Graul, Emilia Sikorska, Magdalena J. Slusarz, Igor Zhukov, Franciszek Kasprzykowski, Agnieszka Kubis, Andrea D. Lipinska, Sylwia Rodziewicz-Motowidlo
Summary: Herpesviruses are common viruses infecting human and animal bodies, with the capability to evade host immune responses by blocking TAP complex. The UL49.5 protein encoded by BoHV-1 acts as a TAP transporter inhibitor, with specific amino acid residues playing crucial roles in its activity.
CHEMISTRY & BIODIVERSITY
(2021)
Article
Virology
Fouad S. El-mayet, Kelly S. Harrison, Clinton Jones
Summary: Studies have shown that productive infection of HSV-1 and BoHV-1 increases the steady-state protein levels of KLF15, which correlates with enhanced virus production.
Article
Microbiology
Chang Liu, Jiayu Lin, Hao Yang, Ningxi Li, Linke Tang, Donna Neumann, Xiuyan Ding, Liqian Zhu
Summary: This study demonstrates that TonEBP (NFAT5) is a potential host factor that restricts bovine herpesvirus 1 (BoHV-1) infection; virus infection controls NFAT5 signaling transduction by altering NFAT5 localization and downstream target expression. Furthermore, the presence of NFAT5 in mitochondria suggests its potential involvement in regulating mitochondrial functions.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Irina Anatolyevna Andrievskaya, Irina Valentinovna Zhukovets, Inna Victorovna Dovzhikova, Nataliya Alexandrovna Ishutina, Ksenia Konstantinovna Petrova
Summary: The research aims to evaluate HSV-1 seropositivity among pregnant women and its impact on pregnancy, childbirth, and newborns. The findings suggest that different titers of HSV-1 antibodies during different trimesters are associated with various complications such as threatened miscarriage, anemia, and placental insufficiency.
Article
Microbiology
Chang Liu, Weifeng Yuan, Hao Yang, Junqing Ni, Linke Tang, Heci Zhao, Donna Neumann, Xiuyan Ding, Liqian Zhu
Summary: Phospholipase C gamma 1 (PLC-gamma 1) plays an important role in the replication process of bovine herpesvirus 1 (BoHV-1), specifically in the Golgi apparatus.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Wenxiao Liu, Jiabing Hong, Jinglong Duan, Bo Jiang, Runan Zhu, Jing Cheng, Ping Wang, Yongqing Li
Summary: In this study, a blocking ELISA was developed to detect neutralizing antibodies against bovine herpesvirus 1 (BoHV-1) using a neutralizing monoclonal antibody (3F8) and protein gD. The assay showed high sensitivity and specificity and a significant correlation with the virus neutralization test. A serological survey in dairy farms in Beijing revealed a high risk of BoHV-1 infection from 2012 to 2017, but protection after implementing an immunization program in 2018.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Chang Liu, Weifeng Yuan, Hao Yang, Junqing Ni, Linke Tang, Heci Zhao, Donna Neumann, Xiuyan Ding, Liqian Zhu
Summary: During productive infection of bovine herpesvirus 1 (BoHV-1), activated form of phospholipase C gamma 1 (PLC-gamma 1), p-PLC-gamma 1(S1248), is partially located in the Golgi apparatus and associated with the viral protein gD. This phosphorylated PLC-gamma 1(S1248) may function as an escort during the trafficking of progeny virions from the Golgi apparatus to the plasma membrane.
MICROBIOLOGY SPECTRUM
(2023)
Article
Agriculture, Dairy & Animal Science
Ana Carolina Garcia Ferreira, Rafael Monteiro Araujo Teixeira, Bhremer Pereira Mendes, Neumara Ribeiro Vaneli, Leticia Fernanda de Oliveira, Paulo Sergio Dornelas Silva, Onofre Barroca de Almeida Neto
Summary: This study aimed to evaluate the effects of two brands of bST on the productive performance of dairy cows and the yield of Minas Padrao cheese. Results showed that the application of bST increased milk production and influenced the physical-chemical composition of milk. However, there were no significant differences in the yield of Minas Padrao cheese between the different treatment groups.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2021)
Article
Microbiology
Caitlyn A. Cowick, Brynnan P. Russ, Anna R. Bales, Bindu Nanduri, Florencia Meyer
Summary: Bovine Respiratory Disease (BRD) is a global multifactorial condition that causes high rates of illness and death in cattle. This study focused on the interaction between BRD pathogens and found that M. haemolytica negatively affects the replication of BoHV-1 in a dose-dependent manner in different in vitro models.
Article
Virology
Austin M. Stults, Patricia J. Sollars, Kelly D. Heath, Sarah J. Sillman, Gary E. Pickard, Gregory A. Smith
Summary: The neuroinvasive properties of alphaherpesviruses are understood through the study of retrograde axonal transport, which involves invasion of peripheral nervous system leading to lifelong latent infections in ganglia. A time-lapse analysis of bovine herpesvirus 1 shows conservation of critical neuroinvasive steps and suggests potential for developing live-attenuated vaccines for enhanced protection of cattle.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Caiquan Zhao, Jie Gao, Yongzhi Wang, Lina Ji, Hui Qin, Wei Hu, Yang Yang
Summary: This study describes a novel recombinant bovine herpes virus type I expressing rabies virus glycoprotein, which induced a protective immune response in mice and cattle.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Kassahun Asmare, Kassaye Aragaw, Berhanu Sibhat, Takele Abayneh, Esayas Gelaye, Belayneh Getachew, Sharon Egan
Summary: This study aimed to estimate the seroprevalence of bovine herpesvirus 1 (BHV-1) and identify potential risk factors in dairy cattle in central and southern Ethiopia. The study showed that BHV-1 had an animal-level seroprevalence of 30.0% and a herd-level seroprevalence of 75.5%. The logistic regression model revealed that adult animals (> 2.5 years) had a higher seroprevalence of BHV-1, and arranging animals in a tail-to-tail fashion appeared to be protective against BHV-1 infection.
PREVENTIVE VETERINARY MEDICINE
(2023)
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.