Journal
VIROLOGY
Volume 512, Issue -, Pages 151-160Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2017.09.012
Keywords
Viral factory; Translational regulation; Vaccinia virus; Nucleoporin; Stress granules
Categories
Funding
- NCCS
- Council of Scientific and Industrial Research (CSIR, India)
- Indian Council of Medical Research (ICMR, India)
- University Grants Commission (UGC, India)
Ask authors/readers for more resources
Vaccinia virus (VACV), a member of the Poxviridae family, uses cytoplasmic factories for its replication. Recent studies indicated that VACV infection requires a set of nucleoporins. However, how the nucleoporins contribute to viral life cycle remains unclear. Here, we report that the nucleoporins Nup62 and Nup358 localize to the cytoplasmic viral factories (VFs). Nup358 was targeted to the VFs at 6 h post-infection (hpi), whereas Nup62, along with the previously reported translation factors such as eIF4E, eIF3 eta and G3BP1, was recruited to the VFs at 8 hpi. Nup358 depletion led to a decrease in the size and number of viral factories and reduction in viral yield. Further studies showed that Nup358 is involved in recruiting Nup62 and eIF4E to the VFs. Collectively, our results reveal spatio-temporal regulation in the recruitment of nucleoporins and translation factors to VFs, and particularly the importance of Nup358 in VACV infection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available