Article
Agriculture, Dairy & Animal Science
Gaia Casalino, Francesco D'Amico, Francesca Rita Dinardo, Giancarlo Bozzo, Valeria Napoletano, Antonio Camarda, Antonella Bove, Roberto Lombardi, Francesco Paolo D'Onghia, Elena Circella
Summary: This study investigated the presence of Campylobacter (C. jejuni and C. coli) in wild birds housed at a wildlife rescue centre. It was found that 24.88% of the birds carried Campylobacter, with higher rates of infection in omnivorous species and indoor aviaries. Interestingly, Campylobacter was not detected in species with body temperatures below 40°C or above 42.2°C. Antibiotic resistance, including multi-drug resistance, was also identified. These findings underscore the importance of increasing biosecurity measures at wildlife rescue centres to reduce health risks to staff involved in wildlife management.
Article
Infectious Diseases
Malgorzata Murawska, Monika Sypecka, Justyna Bartosik, Ewelina Kwiecien, Magdalena Rzewuska, Agnieszka Salamaszynska-Guz
Summary: Research suggests that dogs may serve as a reservoir for diverse, potentially virulent, and antimicrobial-resistant Campylobacter strains.
Article
Multidisciplinary Sciences
Yuli Melisa Sierra-Arguello, Gustavo Perdoncini, Laura Beatriz Rodrigues, Luciana Ruschel dos Santos, Karen Apellanis Borges, Thales Quedi Furian, Carlos Tadeu Pippi Salle, Hamilton Luiz de Souza Moraes, Marcos Jose Pereira Gomes, Vladimir Pinheiro do Nascimento
Summary: Campylobacter jejuni is a common cause of foodborne illnesses worldwide, with limited research on strains isolated from Latin-American countries. This study in Southern Brazil identified 140 C. jejuni strains from various poultry sources and analyzed their virulence markers, providing insight into factors related to the occurrence of this pathogen and their epidemiology. The PCR analysis revealed 25 different virulence profiles, with certain combinations of virulence genes being more common among the strains.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Lauren K. Hudson, William E. Andershock, Runan Yan, Mugdha Golwalkar, Nkuchia M. M'ikanatha, Irving Nachamkin, Linda S. Thomas, Christina Moore, Xiaorong Qian, Richard Steece, Katie N. Garman, John R. Dunn, Jasna Kovac, Thomas G. Denes
Summary: Campylobacteriosis is the most common bacterial foodborne illness in the United States and this study compared clinical and non-clinical Campylobacter populations from Tennessee and Pennsylvania, identified source attribution patterns through phylogenetic analyses, and identified potential outbreak clusters based on genomic similarity.
Article
Microbiology
Hiroshi Asakura, Shiori Yamamoto, Kazuhiro Yamada, Jun Kawase, Hiromi Nakamura, Kou-ichiro Abe, Yoshimasa Sasaki, Tetsuya Ikeda, Ryohei Nomoto
Summary: Campylobacter jejuni and C. coli are major causes of gastrointestinal illnesses transmitted to humans mainly through chicken meats. This study examined the levels of bacterial contamination in retail chicken meats in Japan and found that 45.7% of the samples were positive, with 8.4% exceeding the safe limit. Bacterial counts were higher in fall compared to spring and summer, and younger slaughtered chickens were more contaminated. The study also identified antimicrobial resistance genes and clonal complexes of C. jejuni isolates.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Andrea Laconi, Roberta Tolosi, Ilenia Drigo, Luca Bano, Alessandra Piccirillo
Summary: This study assessed the ability of C. jejuni and C. coli strains isolated from diseased poultry to form biofilm. The results showed that C. jejuni strains were classified as no, weak, and moderate biofilm producers, while all C. coli strains were weak producers. Genes involved in chemotaxis, adhesion, and invasion were present in all C. jejuni strains. Further investigations are needed to better understand the genetic determinants involved in extra-intestinal Campylobacter biofilm formation.
VETERINARY MICROBIOLOGY
(2023)
Article
Microbiology
Cemil Kuerekci, Fatih Sakin, Lennard Epping, Marie-Theres Knuever, Torsten Semmler, Kerstin Stingl
Summary: The study found that thermophilic Campylobacter spp. isolates from wild birds in Turkey, mainly C. jejuni and C. coli, exhibited high genomic diversity and sensitivity to different antibiotics. It was also discovered that there were distinct gene variants in C. coli isolates related to surface structures and signal transduction, indicating niche adaptation of these strains in wild birds.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Malgorzata Andrzejewska, Katarzyna Grudlewska-Buda, Dorota Spica, Krzysztof Skowron, Malgorzata Cwiklinska-Jurkowska, Malgorzata Szady-Grad, Piotr Indykiewicz, Natalia Wiktorczyk-Kapischke, Jacek J. Klawe
Summary: This study aimed to identify the characteristics of Campylobacter isolated from wild birds and water samples, and assess their resistance to antibiotics. The results showed that Campylobacter had virulent properties and high resistance to tetracycline and fluoroquinolones. The lack of genetic relatedness between strains isolated from water and birds suggests other sources of contamination.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Gayani Weerasooriya, Andrea R. McWhorter, Samiullah Khan, Kapil K. Chousalkar
Summary: Campylobacter is the most common bacterial cause of gastrointestinal disease, and contaminated poultry is often the source of infection. This study examines the survivability and virulence potential of sublethally injured Campylobacter in mice, following exposure to commonly used chemicals in poultry meat processing. The results show that sublethally injured Campylobacter jejuni has reduced virulence and colonization potential in mice.
MICROBIOLOGY SPECTRUM
(2022)
Article
Environmental Sciences
Raquel Castillo-Contreras, Marta Marin, Jorge Ramon Lopez-Olvera, Teresa Ayats, Xavier Fernandez Aguilar, Santiago Lavin, Gregorio Mentaberre, Marta Cerda-Cuellar
Summary: Campylobacter and Salmonella are the most commonly reported zoonotic agents in Europe. Wild boars in Barcelona have been found to carry both these pathogens. The prevalence of Campylobacter in wild boars is 61%, with some isolates showing antimicrobial resistance and virulence potential. Salmonella, including a serovar associated with human infections, was also detected in a small percentage of wild boars.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Food Science & Technology
Masaru Usui, Sho Tateno, Masanobu Onozaki, Naoaki Misawa, Yasuhiko Suzuki, Yutaka Tamura, Chie Nakajima
Summary: The rapid method CAMERA can simultaneously detect C. jejuni/coli and determine their susceptibility to fluoroquinolone/macrolide drugs. This study demonstrated the accuracy and efficiency of CAMERA in detecting and confirming antimicrobial resistance, providing a valuable tool for surveillance and control of resistant C. jejuni/coli contamination.
Article
Agriculture, Dairy & Animal Science
Ross C. Beier, J. Allen Byrd, Kathleen Andrews, Denise Caldwell, Tawni L. Crippen, Robin C. Anderson, David J. Nisbet
Summary: The study found that Campylobacter jejuni strains from broiler chicken house environments had low resistance to tetracycline, ciprofloxacin, and nalidixic acid, but resistance to disinfectants like triclosan. Didecyldimethylammonium chloride was the most effective component against C. jejuni among various disinfectants.
Article
Genetics & Heredity
Pedro Panzenhagen, Ana Beatriz Portes, Anamaria M. P. dos Santos, Sheila da Silva Duque, Carlos Adam Conte Junior
Summary: This study analyzed a large number of Campylobacter jejuni genomes to reveal the distribution and prevalence of its virulence factors. The research showed the presence of different groups of virulence factors in these genomes, with some genes being prevalent in over 99.99% of them. Additionally, the study also focused on the distribution of multilocus sequence types.
Article
Microbiology
Ellis Kobina Paintsil, Linda Aurelia Ofori, Charity Wiafe Akenten, Andreas E. Zautner, Joyce Mbwana, Anna Jaeger, Maike Lamshoeft, Juergen May, Kwasi Obiri-Danso, Richard Odame Philipps, Ralf Krumkamp, Denise Dekker
Summary: This study reveals the prevalence of Campylobacter colonization in farm animals, specifically poultry, in the Asante Akim North Municipality of Ghana. The study emphasizes the need for control programs to reduce Campylobacter contamination and antibiotic resistance in the farming sector.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Leon Kemper, Andreas Hensel
Summary: Campylobacter jejuni, causing severe enteritis, has unique features and adapts effectively to environmental conditions. The review summarizes the strategies of C. jejuni for host-pathogen interaction, providing insights for future drug development.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Anna Wozniak-Biel, Aleksandra Podolak
VECTOR-BORNE AND ZOONOTIC DISEASES
(2020)
Article
Veterinary Sciences
Robert Kupczynski, Michal Bednarski, Tomasz Piasecki
VETERINARSKI ARHIV
(2020)
Article
Microbiology
Katarzyna Cwiek, Kamila Korzekwa, Aleksandra Tabis, Jacek Bania, Gabriela Bugla-Ploskonska, Alina Wieliczko
Article
Chemistry, Analytical
Igor Buzalewicz, Agnieszka Suchwalko, Magdalena Karwanska, Alina Wieliczko, Halina Podbielska
Article
Biochemistry & Molecular Biology
Marta Ksiazczyk, Bartlomiej Dudek, Maciej Kuczkowski, Robert O'Hara, Kamila Korzekwa, Anna Wzorek, Agnieszka Korzeniowska-Kowal, Mathew Upton, Adam Junka, Alina Wieliczko, Radoslaw Ratajszczak, Gabriela Bugla-Ploskonska
Summary: This study compared E. coli populations isolated from reptiles, birds, and mammals (humans) for the first time, revealing that most reptilian E. coli strains are nonpathogenic with a low frequency of virulence gene occurrence, differing from the uropathogenic and avian pathogenic strains. Additionally, phylogroups distribution was relatively homogeneous among tested mammalian and avian strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Katarzyna Morka, Ewa Walecka-Zacharska, Justyna Schubert, Bartlomiej Dudek, Anna Wozniak-Biel, Maciej Kuczkowski, Alina Wieliczko, Jaroslaw Bystron, Jacek Bania, Gabriela Bugla-Ploskonska
Summary: The study aimed to evaluate the genetic similarity of Y. enterocolitica strains from various hosts and analyze the prevalence of virulence genes. Different virulence genes were identified among Yersinia sp. strains, with some strains showing potential pathogenicity based on the presence of certain genes. The study also found clustering tendencies among VNTR/PFGE profiles of different origins, suggesting the role of pigs as a potential source of infection for humans.
Article
Biochemistry & Molecular Biology
Pawel Krzyzek, Pawel Migdal, Emil Paluch, Magdalena Karwanska, Alina Wieliczko, Grazyna Gosciniak
Summary: The natural compound myricetin has been shown to slow down the morphological transformation of H. pylori into coccoid forms and reduce biofilm formation. In addition, it can synergistically interact with all five antibiotics commonly used against H. pylori, leading to a significant reduction in their minimum inhibitory concentrations (MICs). Furthermore, myricetin can interfere with the expression of genes related to H. pylori morphogenesis, particularly those involved in the spiral-to-coccoid transition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Igor Buzalewicz, Agnieszka Ulatowska-Jarza, Aleksandra Kaczorowska, Marlena Gasior-Glogowska, Halina Podbielska, Magdalena Karwanska, Alina Wieliczko, Anna K. Matczuk, Katarzyna Kowal, Marta Kopaczynska
Summary: The article introduces a new method for investigating changes in living cells and quantitatively analyzing the process of bacteria photodynamic inactivation. The use of digital holographic tomography (DHT) allows real-time characterization of subcellular structures, biophysical processes, and induced local changes in intracellular density in a label-free manner and at sub-micrometer spatial resolution, which was confirmed by confocal microscopy and statistical analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Magdalena Florek, Urszula Nawrot, Agnieszka Korzeniowska-Kowal, Katarzyna Wlodarczyk, Anna Wzorek, Anna Wozniak-Biel, Magdalena Brzozowska, Jozef Galli, Anna Bogucka, Jaroslaw Krol
Summary: Fungi of Cryptococcus neoformans/C. gattii species complex are pathogens causing severe infections in humans and animals, with a mortality rate of up to 70% in humans. Understanding the population structure and distribution of these pathogens is crucial for studying the epidemiology of cryptococcosis. A study in Poland revealed a population of C. neoformans strains in animals, some of which exhibited resistance to antifungal treatment.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Katarzyna Cwiek, Anna Wozniak-Biel, Magdalena Karwanska, Magdalena Siedlecka, Christine Lammens, Ana Rita Rebelo, Rene S. Hendriksen, Maciej Kuczkowski, Monika Chmielewska-Wladyka, Alina Wieliczko
Summary: The study revealed a low occurrence of mcr-1-positive E. coli strains isolated from Polish poultry, yet all isolated strains demonstrated resistance to multiple antimicrobial agents and the ability to form biofilms at low or medium levels.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Igor Buzalewicz, Iwona Holowacz, Anna K. Matczuk, Mateusz Guzniczak, Dominika Skrzela, Magdalena Karwanska, Alina Wieliczko, Katarzyna Kowal, Agnieszka Ulatowska-Jarza
Summary: The worldwide increase in bacterial resistance and healthcare-associated bacterial infections pose a serious threat to human health. A new therapeutic approach based on the limited delivery of photosensitizer has been proposed, using nanoporous inorganic-organic composites encapsulating Photolon. The material exhibits excellent antimicrobial activity, and the generation of free radicals from the nanoporous surface has been confirmed by scanning Kelvin probe microscopy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Marta Kuzminska-Bajor, Paulina Sliwka, Pawel Korzeniowski, Maciej Kuczkowski, David Saez Moreno, Anna Wozniak-Biel, Emilia Sliwinska, Krzysztof Grzymajlo
Summary: The UPWr_S134 phage cocktail has shown promising results in reducing Salmonella in broiler chickens. The phages in the cocktail remain active under various conditions encountered in the chicken gastrointestinal tract and exhibit strong stability. In mouse and chicken models, the application of the UPWr_S134 phage cocktail delayed infection symptoms and reduced the number of pathogens in internal organs compared to untreated animals.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Magdalena Karwanska, Alina Wieliczko, Anders Miki Bojesen, Kasper Romer Villumsen, Eva Krzyzewska-Dudek, Anna Wozniak-Biel
Summary: This study assessed the prevalence and resistance profile of Gallibacterium anatis bacteria in Polish poultry. The results showed that all investigated isolates were multidrug resistant, with geese isolates having the highest percentage of resistance. The genetic background analysis revealed diverse antimicrobial resistance genes and mobile genetic elements.
VETERINARY RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Aleksandra Antos, Jerzy Rola, Michal Bednarski, Michal Konrad Krzysiak, Julia Kesik-Maliszewska, Magdalena Larska
Summary: The presence of bovine viral diarrhea virus (BVDV) contamination in commercial animal-derived sera and vaccines in Poland was detected, with BVDV-3 being the most common species in sera and only BVDV-1 identified in vaccines. Sequence analysis revealed contamination by six genotypes of BVDV in tested products, emphasizing the importance of monitoring and ensuring the safety of bioproducts at both laboratory and industry levels.
ANNALS OF ANIMAL SCIENCE
(2021)