Journal
ULTRASONICS SONOCHEMISTRY
Volume 37, Issue -, Pages 518-528Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ultsonch.2017.02.010
Keywords
Ultrasound; Candida tropicalis; Cell growth; Intracellular calcium ions concentration; mRNA expression profiles
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Funding
- National Primary Research & Developement Plan [2016YFD0401401]
- Primary Research & Developement Plan of Jiangsu Province [BE2016352]
- Special Fund of China Postdoctoral Science Foundation [201003560]
- Startup Scientific Research Fund from Jiangsu University for Advanced Professionals [08JDG035]
- Qing Lan Project of Jiangsu province
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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Here we report the enhancement of both cellular permeability and cell growth rate of Candida tropicalis after treatment with the ultrasonic irradiation of low intensity using a mode of sweeping frequency (UILS) generated by a self-developed ultrasonic device in our lab. After the ultrasonic treatment, remarkable biomass enhancement of the yeast was observed; the hyphae became significantly longer; the seeped cellular protein and nucleic acid from the yeast increased and the cellular Cat(2+) content became lower. Illumina transcriptome sequencing showed that the ultrasonic treatment affected the expression of genes involved in diverse cellular components, biological processes and molecular functions. RT-PCR and Western blotting further confirmed the up-/down-regulation of genes in the ultrasound-treated yeasts. The optimal conditions of the ultrasonic treatment for the maximum biomass addition were determined as follows: the yeast was treated for 1 h at the mid logarithmic phase, the frequency was 28 +/- 2 kHz and the power density was 120 W/L. Under these conditions, the Candida tropicalis biomass increased by 142.5% compared with the untreated yeast. (C) 2017 Elsevier B.V. All rights reserved.
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