Article
Multidisciplinary Sciences
Tiantian Wang, Peilong Li, Qiuchen Qi, Shujun Zhang, Yan Xie, Jing Wang, Shibiao Liu, Suhong Ma, Shijun Li, Tingting Gong, Huiting Xu, Mengqiu Xiong, Guanghua Li, Chongge You, Zhaofan Luo, Juan Li, Lutao Du, Chuanxin Wang
Summary: The study shows that DNA methylation plays an important role in the immune alterations of breast cancer. Researchers have identified four specific methylation markers in peripheral blood mononuclear cells and developed an efficient and convenient multiplex methylation-specific quantitative PCR assay for early detection of breast cancer. This assay outperforms existing clinical diagnostic methods, especially in the detection of early-stage and minimal tumors.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Jin Song, Lori J. Sokoll, Daniel W. Chan, Zhen Zhang
Summary: A study identified a two-marker panel (CA19-9 and CA-125) that significantly improved the detection performance of PDAC. Another two-marker panel (HE4 and CEA) also outperformed CA19-9 in distinguishing IPMN from chronic pancreatitis. These biomarker panels showed potential complementarity in detecting early PDAC, and further clinical validation is warranted.
Article
Chemistry, Analytical
Xue Chen, Hongjie Song, Ziyan Li, Rui Liu, Yi Lv
Summary: This study achieved multiple evaluations of breast cancer biomarkers in human serum samples by designing and synthesizing multiple lanthanide nanoparticle tags, demonstrating wide linear ranges and low detection limit levels for all biomarkers in clinical applications.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Biomaterials
Kulrisa Kuntamung, Jaroon Jakmunee, Kontad Ounnunkad
Summary: The newly developed electrochemical immunosensor can simultaneously detect three early breast cancer biomarkers with high reliability, low detection limits, and high selectivity, making it suitable for tumor marker detection in human serum.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Biochemistry & Molecular Biology
Diogo J. Silva, Goncalo Miranda, Teresina Amaro, Matilde Salgado, Alexandra Mesquita
Summary: Tumor budding (TB) is a dynamic process associated with the epithelial-mesenchymal transition and has been proven to be a significant prognostic biomarker for colorectal cancer. This study aimed to investigate the clinical applicability and prognostic value of TB in patients with early breast cancer (EBC). The results showed a significant association between high TB score and aggressive clinicopathological features, as well as poor prognosis. The findings suggest that TB could serve as a valuable prognostic biomarker for EBC.
Article
Oncology
Kathleen M. Mahoney, Petra Ross-Macdonald, Long Yuan, Linan Song, Eliseo Veras, Megan Wind-Rotolo, David F. McDermott, F. Stephen Hodi, Toni K. Choueiri, Gordon J. Freeman
Summary: This study investigated the levels of soluble PD-L1 (sPD-L1) before and during nivolumab treatment in metastatic clear cell renal cell carcinoma (RCC) and melanoma patients. The results showed that sPD-L1 levels were associated with worse prognosis and clinical factors. Additionally, changes in sPD-L1 levels during treatment were linked to treatment outcomes.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Oncology
Qin Huo, Zhenwei Li, Siqi Chen, Juan Wang, Jiaying Li, Ni Xie
Summary: In this study, we found that VWCE expression was significantly lower in breast cancer tissues compared to normal tissues, and it was associated with subtypes and receptor statuses of breast cancer patients. VWCE expression was also correlated with the activation or inhibition of multiple oncogenic pathways, and with the immune infiltration levels of Th1 and M2 macrophages in breast cancer. These findings suggest that VWCE could be a potential prognostic biomarker and therapeutic target in breast cancer.
CANCER CELL INTERNATIONAL
(2021)
Article
Cell Biology
Yutian Zou, Jindong Xie, Wenwen Tian, Linyu Wu, Yi Xie, Shanshan Huang, Yuhui Tang, Xinpei Deng, Hao Wu, Xinhua Xie
Summary: This study comprehensively analyzed the expression, alteration, prognosis, and biological functions of various KCNK genes in breast cancer. It found that the expression of certain KCNK genes was correlated with the prognosis of breast cancer patients and some genes had a high mutation rate. A prognostic signature with predictive performance was established, and a nomogram combining KCNK-based risk score with clinical features was constructed. In addition, KCNKs were associated with the activation of tumor microenvironment cells and may regulate breast cancer progression by modulating immune response.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Immunology
Marco Antonio Fonseca-Montano, Karla Itzel Vazquez-Santillan, Alfredo Hidalgo-Miranda
Summary: Breast cancer is a common and deadly malignancy in women, with alterations in genes and signaling pathways playing a significant role. Abnormal expression of long non-coding RNAs (lncRNAs) affects the tumor immune microenvironment and behavior of breast cancer. This review highlights the role of lncRNAs as modulators of the antitumoral immune response and immune microenvironment in breast cancer, and suggests their potential as biomarkers and targets for immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Xiaoyan Zuo, Sixin Ren, He Zhang, Jianfei Tian, Ruinan Tian, Baoai Han, Hui Liu, Qian Dong, Zhiyong Wang, Yanfen Cui, Ruifang Niu, Fei Zhang
Summary: This study investigates the expression and function of ACE2 in breast cancer cells, and finds that ACE2 plays an important role in the sensitivity to chemotherapy drugs and prognosis of breast cancer patients.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Chemistry, Analytical
Bing Zhang, Xue Wang, Yan Cheng
Summary: A photochromic immunoassay based on ZnO/AgI nanophotocatalyst was developed for tumor marker detection. The color development is achieved by the oxidation of tetramethyl benzidine (TMB) solution by ZnO/AgI nanomaterials driven by visible light irradiation. A sandwich-type immunoassay using ZnO/AgI nanomaterial as labels showed good performance for the detection of carcinoma embryonic antigen (CEA).
Review
Medicine, Research & Experimental
You Shuai, Zhonghua Ma, Jie Ju, Tong Wei, Songlin Gao, Yikun Kang, Zixuan Yang, Xue Wang, Jian Yue, Peng Yuan
Summary: In recent years, liquid biomarkers have gained significant attention in tumor research, with a focus on non-blood sources that provide unique advantages and supplementary information in certain situations. This article summarizes the latest advances in the analysis of non-blood biomarkers and discusses their advantages, limitations, and appropriate use in different scenarios.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Oncology
Jamie M. Sperger, Hamid Emamekhoo, Rana R. McKay, Charlotte N. Stahlfeld, Anupama Singh, Xinyi E. Chen, Lucia Kwak, Cole S. Gilsdorf, Serena K. Wolfe, Xiao X. Wei, Rebecca Silver, Zhenwei Zhang, Michael J. Morris, Glenn Bubley, Felix Y. Feng, Howard Scher, Dana Rathkopf, Scott M. Dehm, Toni K. Choueiri, Susan Halabi, Andrew J. Armstrong, Alexander W. Wyatt, Mary-Ellen Taplin, Shuang G. Zhao, Joshua M. Lang
Summary: This study utilized a novel liquid biopsy technology to analyze mRNA from circulating tumor cells, identifying mechanisms of resistance to androgen receptor signaling inhibitors in prostate cancer. The expression of AR-Vs, AR genomic structural alterations, and neuroendocrine prostate cancer markers were found to play crucial roles in therapy optimization. The research demonstrated that a specific transcriptional profile in CTCs can serve as an independent prognostic marker, beyond AR-V7, in metastatic prostate cancer patients, helping to identify various ARSI resistance mechanisms.
JOURNAL OF CLINICAL ONCOLOGY
(2021)
Article
Biochemical Research Methods
Bo Dai, Cheng Yin, Jiandong Wu, Wei Li, Lulu Zheng, Francis Lin, Xiaodian Han, Yongfeng Fu, Dawei Zhang, Songlin Zhuang
Summary: Microfluidics plays a crucial role in advancing portable and cost-effective bioanalytical diagnostics. A pump-free microfluidic chip has been developed for rapid and sensitive detection of multiple biomarkers, offering potential applications in disease monitoring.
Article
Biochemistry & Molecular Biology
Sanghyun Roh, Sooyoun Kim, Inpyo Hong, Minho Lee, Han Jo Kim, Tae Sung Ahn, Dong Hyun Kang, Moo-Jun Baek, Hyoung Jong Kwak, Chang-Jin Kim, Dongjun Jeong
Summary: In this study, the TSPAN5 gene was identified as an important biomarker for predicting the prognosis of patients with colorectal cancer (CRC). Suppression of TSPAN5 was found to decrease cancer cell proliferation, migration, invasion, and tumorigenicity, suggesting its potential as a therapeutic target. Evaluating TSPAN5 expression may help in prognosis observation and treatment determination for CRC patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Thomas Ostendorf, Thomas Zillinger, Katarzyna Andryka, Thais Marina Schlee-Guimaraes, Saskia Schmitz, Samira Marx, Kubra Bayrak, Rebecca Linke, Sarah Salgert, Julia Wegner, Tatjana Grasser, Sonja Bauersachs, Leon Soltesz, Marc P. Huebner, Maximilian Nastaly, Christoph Coch, Matthias Kettwig, Ingo Roehl, Marco Henneke, Achim Hoerauf, Winfried Barchet, Jutta Gaertner, Martin Schlee, Gunther Hartmann, Eva Bartok
Article
Pharmacology & Pharmacy
Sandra Kalthoff, Stefan Paulusch, Alexander Rupp, Stefan Holdenrieder, Gunther Hartmann, Christian P. Strassburg
BRITISH JOURNAL OF PHARMACOLOGY
(2020)
Review
Immunology
Eva Bartok, Gunther Hartmann
Article
Medicine, Research & Experimental
Lina Such, Fang Zhao, Derek Liu, Beatrice Thier, Vu Thuy Khanh Le-Trilling, Antje Sucker, Christoph Coch, Natalia Pieper, Sebastian Howe, Hilal Bhat, Halime Kalkavan, Cathrin Ritter, Robin Brinkhaus, Selma Ugurel, Johannes Koester, Ulrike Seifert, Ulf Dittmer, Martin Schuler, Karl S. Lang, Thomas A. Kufer, Gunther Hartmann, Jurgen C. Becker, Susanne Horn, Soldano Ferrone, David Liu, Eliezer M. Van Allen, Dirk Schadendorf, Klaus Griewank, Mirko Trilling, Annette Paschen
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Article
Virology
Ramona Dolscheid-Pommerich, Eva Bartok, Marcel Renn, Beate M. Kuemmerer, Bianca Schulte, Ricarda M. Schmithausen, Birgit Stoffel-Wagner, Hendrik Streeck, Sandra Saschenbrecker, Katja Steinhagen, Gunther Hartmann
Summary: This study analyzed the correlation between binding IgG and neutralizing antibodies in COVID-19 patients, revealing a strong positive relationship and supporting the use of QuantiVac ELISA to assess protective immunity post-infection or vaccination.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Yifat Ofir-Birin, Hila Ben Ami Pilo, Abel Cruz Camacho, Ariel Rudik, Anna Rivkin, Or-Yam Revach, Netta Nir, Tal Block Tamin, Paula Abou Karam, Edo Kiper, Yoav Peleg, Reinat Nevo, Aryeh Solomon, Tal Havkin-Solomon, Alicia Rojas, Ron Rotkopf, Ziv Porat, Dror Avni, Eli Schwartz, Thomas Zillinger, Gunther Hartmann, Antonella Di Pizio, Neils Ben Quashie, Rivka Dikstein, Motti Gerlic, Ana Claudia Torrecilhas, Carmit Levy, Esther N. M. Nolte-'t Hoen, Andrew G. Bowie, Neta Regev-Rudzki
Summary: The study reveals that Plasmodium falciparum inhibits CXCL10 protein translation in monocytes by releasing extracellular vesicles loaded with RNAs, thus using CXCL10 concentration to control its survival strategy.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Johannes Braegelmann, Carina Lorenz, Sven Borchmann, Kazuya Nishii, Julia Wegner, Lydia Meder, Jenny Ostendorp, David F. Ast, Alena Heimsoeth, Takamasa Nakasuka, Atsuko Hirabae, Sachi Okawa, Marcel A. Dammert, Dennis Plenker, Sebastian Klein, Philipp Lohneis, Jianing Gu, Laura K. Godfrey, Jan Forster, Marija Trajkovic-Arsic, Thomas Zillinger, Mareike Haarmann, Alexander Quaas, Stefanie Lennartz, Marcel Schmiel, Joshua D'Rozario, Emily S. Thomas, Henry Li, Clemens A. Schmitt, Julie George, Roman K. Thomas, Silvia von Karstedt, Gunther Hartmann, Reinhard Buettner, Roland T. Ullrich, Jens T. Siveke, Kadoaki Ohashi, Martin Schlee, Martin L. Sos
Summary: Kinase inhibitors are commonly used in cancer treatment, but resistance can develop. This study explores how activating RIG-I can enhance cancer cell death when combined with kinase inhibition, potentially reducing treatment resistance and promoting tumor shrinkage. By understanding the mechanisms behind intratumoral reprogramming, the efficacy of targeted drugs in genetically defined cancer patients may be prolonged.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Ruben Serrano, Christoph Coch, Christian Peters, Gunther Hartmann, Daniela Wesch, Dieter Kabelitz
Summary: The study investigated the co-stimulatory role of selected TLR ligands on gamma delta T-cell activation, finding that TLR7 and TLR8 RNA ligands, along with pyrophosphate antigens, induced rapid cytokine expression in V delta 2 T cells and required accessory cells for activation. The adjuvant activity of TLR8 and TLR7/8 RNA ligands in a cell context-dependent manner may be important for optimizing gamma delta T-cell based cancer immunotherapy in the future.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Jernej Pusnik, Enrico Richter, Bianca Schulte, Ramona Dolscheid-Pommerich, Christian Bode, Christian Putensen, Gunther Hartmann, Galit Alter, Hendrik Streeck
Summary: Memory B cells specific to the spike protein of SARS-CoV-2 are generated in smaller numbers but with superior function in individuals recovering from mild COVID-19, dependent on IL-21(+)CD4(+) T cells. In contrast, severely ill COVID-19 patients generate fewer efficient memory B cells with dependence on CD40L(+)CD4(+) T cells. Activation and exhaustion of memory B cells during COVID-19 are linked to CD4(+) T cell functions, with CD4(+) T cells recognizing membrane proteins showing stronger association with spike-specific memory B cells.
Article
Biology
Sergej Franz, Fabian Pott, Thomas Zillinger, Christiane Schueler, Sandra Dapa, Carlo Fischer, Vania Passos, Saskia Stenzel, Fangfang Chen, Katinka Doehner, Gunther Hartmann, Beate Sodeik, Frank Pessler, Graham Simmons, Jan Felix Drexler, Christine Goffinet
Summary: IFITM proteins restrict entry and spread of chikungunya virus and Mayaro virus, with CHIKV showing antagonism against IFITMs. CHIKV infection may lead to posttranscriptional regulation of IFITM3, resulting in reduced antiviral activity.
LIFE SCIENCE ALLIANCE
(2021)
Article
Immunology
Annemarie Steiner, Thomas Reygaerts, Alessandra Pontillo, Isabella Ceccherini, Jonas Moecking, Fiona Moghaddas, Sophia Davidson, Francesco Caroli, Alice Grossi, Fabio Fernandes Morato Castro, Jorge Kalil, Florian N. Gohr, Florian Schmidt, Eva Bartok, Thomas Zillinger, Gunther Hartmann, Matthias Geyer, Marco Gattorno, Leonardo Oliveira Mendonca, Seth L. Masters
Summary: NLRC4 (A160T) can lead to recessively inherited autoinflammation and immune dysregulation, or function as a heterozygous risk factor for the development of ulcerative colitis.
JOURNAL OF CLINICAL IMMUNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Marcel Renn, Eva Bartok, Thomas Zillinger, Gunther Hartmann, Rayk Behrendt
Summary: The global spread of infections caused by the Coronavirus SARS-CoV-2 has highlighted the urgent need for novel prophylactic and therapeutic interventions to reduce health risks associated with the pandemic. Animal models are crucial for assessing the efficacy and safety of potential new antivirals and vaccines.
PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Multidisciplinary Sciences
Matthias Kettwig, Katharina Ternka, Kristin Wendland, Dennis Manfred Krueger, Silvia Zampar, Charlotte Schob, Jonas Franz, Abhishek Aich, Anne Winkler, M. Sadman Sakib, Lalit Kaurani, Robert Epple, Hauke B. Werner, Samy Hakroush, Julia Kitz, Marco Prinz, Eva Bartok, Gunther Hartmann, Simone Schroeder, Peter Rehling, Marco Henneke, Susann Boretius, A. Alia, Oliver Wirths, Andre Fischer, Christine Stadelmann, Stefan Nessler, Jutta Gaertner
Summary: Infantile-onset RNaseT2 deficient leukoencephalopathy is characterized by cystic brain lesions, white matter alterations, cerebral atrophy, and psychomotor impairment. Studies using Rnaset2(-/-) mice reveal upregulation of interferon-stimulated genes, IFNAR1-dependent neuroinflammation, and insights into hippocampal-accentuated brain atrophy and cognitive impairment. This study provides a potential animal model for investigating CNS damage associated with RNaseT2 deficiency and exploring therapeutic interventions for interferon-driven brain pathology.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Theophilos Tzaridis, Daniel Bachurski, Shu Liu, Kristin Surmann, Felix Babatz, Manuela Gesell Salazar, Uwe Voelker, Michael Hallek, Ulrich Herrlinger, Ina Vorberg, Christoph Coch, Katrin S. Reiners, Gunther Hartmann
Summary: Extracellular vesicles (EVs) are valuable sources of biomarkers, but their clinical use requires simple, comparable, and reproducible analytical methods. A comprehensive analysis of EV separation techniques found that combining size exclusion chromatography (SEC) with ultracentrifugation yields the highest purity and functionality of EVs for biomarker studies from human serum or plasma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Enrico Richter, Doaa Al Arashi, Bianca Schulte, Christian Bode, Benjamin Marx, Souhaib Aldabbagh, Celina Schlueter, Beate Mareike Kuemmerer, Johannes Oldenburg, Markus B. Funk, Christian Putensen, Ricarda Maria Schmithausen, Gunther Hartmann, Anna Eis-Huebinger, Hendrik Streeck
Summary: Analysis of 92 blood samples from individuals infected with SARS-CoV-2 revealed absence of SARS-CoV-2 RNA in mild to asymptomatic patients, while elevated neutralizing capacity was observed in severe patients with higher anti-spike IgG antibodies, supporting the safety of blood and plasma transfusion from mild or asymptomatic individuals.
TRANSFUSION MEDICINE AND HEMOTHERAPY
(2021)