Journal
TRENDS IN GENETICS
Volume 33, Issue 5, Pages 322-335Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tig.2017.02.003
Keywords
-
Categories
Funding
- W.M. Keck Foundation
- NIH [R35GM119728]
- Boettcher Foundation's Webb-Waring Biomedical Research Program
Ask authors/readers for more resources
Antibody derivatives, such as antibody fragments (Fabs) and single-chain variable fragments (scFvs), are now being used to image traditionally hard-to-see protein subpopulations, including nascent polypeptides being translated and post-translationally modified proteins. This has allowed researchers to directly image and quantify, for the first time, translation initiation and elongation kinetics with single-transcript resolution and the temporal ordering and kinetics of post-translational histone and RNA polymerase II modifications. Here, we review these developments and discuss the strengths and weaknesses of live-cell imaging with antibody-based probes. Further development of these probes will increase their versatility and open new avenues of research for dissecting complex gene regulatory dynamics.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available