Toxicological interactions of silver nanoparticles and non-essential metals in human hepatocarcinoma cell line

Toxicological interaction represents a challenge to toxicology, particularly for novel contaminants. There are no data whether silver nanoparticles (AgNPs), present in a wide variety of products, can interact and modulate the toxicity of ubiquitous contaminants, such as nonessential metals. In the current study, we investigated the toxicological interactions of AgNP (size = 1-2 nm; zeta potential = 23 mV), cadmium and mercury in human hepatoma HepG2 cells. The results indicated that the co-exposures led to toxicological interactions, with AgNP + Cd being more toxic than AgNP + Hg. Early (2-4 h) increases of ROS (DCF assay) and mitochondria( O-2 center dot(-) levels (Mitosox assay) were observed in the cells co-exposed to AgNP + Cd/Hg, in comparison to central and individual contaminants, but the effect was partially reverted in AgNP + Hg at the end of 24 h-exposure. In addition, decreases of mitochondrial metabolism (VITT), cell viability (neutral red uptake assay), cell proliferation (crystal violet assay) and ABC-transporters activity (rhodamine accumulation assay) were also more pronounced in the co-exposure groups. Foremost, co-exposure to AgNP and metals potentiated cell death (mainly by necrosis) and Hg2+ (but not Cd2+) intracellular levels (ICP-MS). Therefore, toxicological interactions seem to increase the toxicity of AgNP, cadmium and mercury. (C) 2017 Elsevier Ltd. All rights reserved.