Article
Engineering, Biomedical
Omar Alheib, Lucilia P. da Silva, Alain da Silva Morais, Katia A. Mesquita, Rogerio P. Pirraco, Rui L. Reis, Vitor M. Conrelo
Summary: We developed an injectable gellan gum-based hydrogel that delivers C2C12 into a localized myopathic model. The gellan gum was biofunctionalized with a laminin-derived peptide to mimic the natural muscular extracellular matrix. Furthermore, the hydrogel was physically adjusted to replicate the mechanical properties of native tissue. This formula was utilized for the first time in the field of skeletal muscle tissue regeneration. The injectability of the hydrogel allowed for non-invasive administration, creating a reliable microenvironment that supported C2C12 with minimal inflammation. The treatment of skeletal muscle defects with the cell-laden hydrogel significantly enhanced regeneration of localized muscular trauma.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Omar Alheib, Lucilia P. da Silva, David Caballero, Ricardo A. Pires, Subhas C. Kundu, Vitor M. Correlo, Rui L. Reis
Summary: Current therapies for skeletal muscle disorders/injuries are limited, necessitating the development of new treatments. Skeletal muscle tissue engineered platforms could provide valuable insights into the pathophysiology of skeletal muscle disorders/injuries and the efficacy of new therapies.
Article
Engineering, Biomedical
Omar Alheib, Lucilia P. da Silva, Katia A. Mesquita, Alain da Silva Morais, Rogerio P. Pirraco, Rui L. Reis, Vitor M. Correlo
Summary: This study evaluated the potential of human adipose-derived mesenchymal stem cells (hASCs) pre-cultured in gellan gum based spongy-like hydrogels (SLHs). In vitro, hASCs were found to proliferate, release growth factors and cytokines important for muscular regeneration. In vivo, the SLHs with hASCs showed promoting effects on angiogenesis, neoinnervation, and the expression of myogenic proteins in a volumetric muscle loss (VML) mouse model.
BIOMEDICAL MATERIALS
(2023)
Article
Chemistry, Applied
Brianda Maria Salazar Salas, Genesis Adilene Grijalva Bustamante, Daniel Fernandez Quiroz, Maria Monica Castillo Ortega, Jose Carmelo Encinas, Pedro Jesus Herrera Franco, Teresa del Castillo Castro
Summary: The development of stimuli-responsive release systems as functional platforms for controlled drug delivery is an important contribution to modern medicine. In this study, electroactive nanocomposite hydrogels were prepared by encapsulating green-synthesized polypyrrole (PPy) colloids within bioamine-crosslinked gellan gum (GG) networks. The hydrogels exhibited an interconnected porous structure and their properties were dependent on the filler content. The release of ibuprofen (IBP) from the hydrogels was significantly activated by electrical stimuli, demonstrating their potential for electrically-controlled drug delivery.
REACTIVE & FUNCTIONAL POLYMERS
(2022)
Article
Multidisciplinary Sciences
Sherzod Shukhratovich Abdullaev, Raed H. Althomali, Ebraheem Abdu Musad Saleh, Magizov Rustem Robertovich, I. B. Sapaev, Rosario Mireya Romero-Parra, Hashem O. Alsaab, M. Abdulfadhil Gatea, Mohammed N. Fenjan
Summary: In this study, a GG-AG hydrogel/g-C3N4/ZnCuFe2O4 nanobiocomposite with excellent antibacterial activity and complete biocompatibility was prepared. The nanobiocomposite was able to regulate its mechanical behavior and enhance its properties by incorporating g-C3N4 and metal oxide nanoparticles. The nanobiocomposite exhibited spherical morphology, high biocompatibility, and the ability to inhibit bacterial growth, as well as good water absorption capability and mechanical properties. Therefore, the GG-AG hydrogel/g-C3N4/ZnCuFe2O4 nanobiocomposite holds potential for biomedical applications such as tissue engineering and wound healing.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Applied
Zhiping Fan, Ping Cheng, Yan Gao, Dawei Wang, Guangwei Jia, Pan Zhang, Sangeeta Prakash, Zhengping Wang, Jun Han
Summary: This research highlights the engineering of novel composite hydrogels from natural polysaccharides Salecan and Gellan using physical methods, without any structural modification or chemical crosslinking. The rheological properties of the hydrogels were investigated and key parameters were explored and compared using different models like Power Law, Herschel-Bulkley, and Arrhenius. This study reports the development of a novel composite hydrogel with ideal elasticity, injectability, good thermostability, and self-recovery, paving the way for its application in various fields.
FOOD HYDROCOLLOIDS
(2022)
Article
Engineering, Chemical
Meilin Chen, Peng Yu, Chuanbei Ao, Miao Zhang, Jiaqi Xing, Chunmei Ding, Jing Xie, Jianshu Li
Summary: This research presents a new design for solvent-responsive polysaccharide hydrogel materials by preparing hydrogels that exhibit solvent-responsive properties to ethanol. This expands the possibilities for the application of solvent-responsive polysaccharide hydrogels.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2022)
Article
Chemistry, Applied
Shanyi Wu, Rongjun Xiao, Yong Wu, Laijun Xu
Summary: This review discusses the emerging advances and applications of Gellan gum (GG)-based hydrogels in tissue engineering, highlighting their potential in bone, cartilage, nerve, and retina tissue engineering. The review also explores the use of GG-based hydrogels as bioink components for 3D bioprinting.
CARBOHYDRATE POLYMERS
(2024)
Article
Biochemistry & Molecular Biology
Maryam Sahraro, Mehdi Barikani, Hamed Daemi, Payam Baei
Summary: In this study, multifunctional polyurethane nanoparticles (MPUNs) were embedded into methacrylated gellan gum to prepare stimuli-responsive hydrogels with improved mechanical properties. The optimized formulation showed excellent compression strength and ultimate strain, as well as the ability to restore its original shape after repeated compression tests. The hydrogels exhibited a dual physical and chemical crosslinking mechanism and were found to be non-toxic to human dermal fibroblast cells, making them suitable for soft tissue engineering applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Applied
Linyuan Liu, Duoduo Zhang, Xiaoxiao Song, Mi Guo, Ziwei Wang, Fang Geng, Xingtao Zhou, Shaoping Nie
Summary: In this study, the encapsulation of anthocyanins with Gelatin/Gellan gum was investigated to protect anthocyanins from degradation in the stomach and enhance their release in the small intestine. The results showed that Gelatin/Gellan gum = 8/2 had the most compact and stable structure, and the compound hydrogels did not inhibit the proliferation of normal rat intestinal epithelial cells (IEC-6), indicating the effectiveness and safety of this method.
FOOD HYDROCOLLOIDS
(2022)
Article
Polymer Science
Calogero Fiorica, Giuseppina Biscari, Fabio Salvatore Palumbo, Giovanna Pitarresi, Annalisa Martorana, Gaetano Giammona
Summary: A series of four different low molecular weight gellan gum products were obtained by alkaline hydrolysis to investigate their impact on rheological properties and physicochemical characteristics of ionotropic crosslinked hydrogels. Thermo-rheological analysis and strain sweep experiments were used to study the properties of the polysaccharide aqueous dispersions. The effect of different Ca2+ ions on the rheological properties of hydrogels, as well as their morphology, swelling behavior, and viscoelastic properties, were also studied.
Article
Biochemistry & Molecular Biology
Seyed Saeed Mousavi, Hamid Keshvari, Hamed Daemi
Summary: This study demonstrates that partial sulfation of Gellan gum (GG) can produce a cytocompatible temperature-responsive hydrogel. The resulting hydrogel shows an interesting thermoreversibility at 42°C and a drop in mechanical properties can be compensated by further calcium-mediated ionic crosslinking.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Polymer Science
Shuwen Zhou, Xinmeng Zheng, Ke Yi, Xuancheng Du, Cheng Wang, Pengfei Cui, Pengju Jiang, Xinye Ni, Lin Qiu, Jianhao Wang
Summary: This study synthesized a glutathione-gellan gum conjugate (GSH-GG) as a responsive gelling material for delivering doxorubicin hydrochloride (DOX) for cancer therapy. DOX-loaded GSH-GG showed temperature-ion dual responsive gelling property and lower drug release under acidic conditions, with selective action on cancer cells and potential for complete inhibition of tumor progression.
Article
Biochemistry & Molecular Biology
Giuseppina Biscari, Yanmiao Fan, Faridah Namata, Calogero Fiorica, Michael Malkoch, Fabio Salvatore Palumbo, Giovanna Pitarresi
Summary: This study develops hybrid hydrogels based on the formation of a polyelectrolyte complex between dopamine-functionalized Gellan Gum and the TMP-G2-alanine dendrimer. The hydrogels can be doubly crosslinked with CaCl2 to obtain solid hydrogels, or oxidized to obtain injectable hydrogels. The hydrogels exhibit cell viability up to 100% and broad-spectrum antimicrobial activity.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Polymer Science
Dariya Getya, Ivan Gitsov
Summary: This study aimed to synthesize novel semi-interpenetrating networks and amphiphilic conetworks by combining hydrophilic soft matter (Gellan Gum, GG) with hydrophobic rigid poly(styrene), PSt. The resulting conetworks showed improved thermal and mechanical properties compared to pure poly(styrene) and pure GG gels. These conetworks also exhibited high binding ability towards several organic dyes, suggesting their potential use in environmental clean-up.
Article
Cell & Tissue Engineering
Ines Bartolo, Rui L. Reis, Alexandra P. Marques, Mariana T. Cerqueira
Summary: Wound re-epithelialization is a dynamic process involving the active signaling network between growth factors and various cell types, with keratinocyte growth factor (KGF) playing a central role in promoting migration and proliferation of keratinocytes. Therapeutic strategies involving delivery of KGF, such as controlled release from biomaterials and gene delivery techniques, have shown great effectiveness in wound closure.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Review
Biotechnology & Applied Microbiology
Carla M. Abreu, Alexandra P. Marques
Summary: Regeneration of hair follicles faces challenges such as loss of trichogenic capacity in culture and lack of suitable human-based in vitro testing platforms.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Rita I. R. Ibanez, Ronaldo J. F. C. do Amaral, Christopher R. Simpson, Sarah M. Casey, Rui L. Reis, Alexandra P. Marques, Ciara M. Murphy, Fergal J. O'Brien
Summary: This study successfully incorporated PRP into a bioink for 3D printing regenerative scaffolds with controlled release of growth factors, showing superior angiogenic potential without inducing myofibroblastic phenotype in fibroblasts. This disruptive technology allows for personalized treatment with enhanced vascularization in tissue engineering applications.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Engineering, Biomedical
Jessica S. Yamanaka, Ana Clara Oliveira, Ana Raquel Bastos, Emanuel M. Fernandes, Rui L. Reis, Vitor M. Correlo, Antonio Carlos Shimano
Summary: The study characterized a bovine peridcardium collagen membrane and evaluated its effectiveness in bone defect regeneration in rat tibia. The membrane demonstrated favorable characteristics for long bone repair and enhanced bone tissue growth.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2023)
Article
Nutrition & Dietetics
Eirini K. Kydonaki, Laura Freitas, Henrique Reguengo, Carlos Raposo Simon, Ana R. Bastos, Emanuel M. Fernandes, Raphael F. Canadas, Joaquim M. Oliveira, Vitor M. Correlo, Rui L. Reis, Maria Vliora, Paraskevi Gkiata, Yiannis Koutedakis, Georgia Ntina, Rui Pinto, Andres E. Carrillo, Franklim Marques, Tania Amorim
Summary: This study compared the effects of different interventions on osteoporosis and found that supplementation with bovine colostrum may have positive effects on bone formation, but is not as effective as alendronate.
Review
Biotechnology & Applied Microbiology
Omar Alheib, Lucilia P. da Silva, Il Keun Kwon, Rui L. Reis, Vitor M. Correlo
Summary: This review examines the potential of tissue-engineered surrogates in promoting muscle healing by critically analyzing data from recent preclinical models. It discusses the therapeutic advantages provided by a combination of different biomaterials, cell types, and biochemical mediators. The current therapies on muscle healing are summarized, highlighting their main advantages and drawbacks. Previous and ongoing clinical trials are also discussed, along with future directions for the field.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Helena R. Moreira, Daniel B. Rodrigues, Sara Freitas-Ribeiro, Lucilia P. da Silva, Alain da S. Morais, Mariana Jarnalo, Ricardo Horta, Rui L. Reis, Rogerio P. Pirraco, Alexandra P. Marques
Summary: This study demonstrates the ability of αvβ3 integrin-specific biomaterials to trigger vasculogenesis by activating essential pathways for endothelial cell survival and migration. This strategy is of great significance for vascularization of tissue engineering constructs.
NPJ REGENERATIVE MEDICINE
(2022)
Article
Chemistry, Analytical
Diana Meira, Manuela Proenca, Rita Rebelo, Ana Barbosa, Marco S. Rodrigues, Joel Borges, Filipe Vaz, Rui L. Reis, Vitor M. Correlo
Summary: In this study, gold nanoparticles were dispersed into chitosan membranes and used as plasmonic platforms for molecular sensing. The membranes showed stable optical response and high sensitivity to changes in surrounding media's refractive index. Despite the need for further material optimization, the chitosan/gold nanoparticles membranes demonstrated potential for LSPR-based sensing applications.
Review
Food Science & Technology
Diana I. Meira, Ana I. Barbosa, Joel Borges, Rui L. Reis, Vitor M. Correlo, Filipe Vaz
Summary: Global population growth has a significant impact on the global food industry, posing a threat to food safety and quality. Mycotoxins, particularly Ochratoxin-A (OTA), are produced by fungus that contaminates various food species and products. OTA not only endangers food production but also poses potential toxicological risks to humans, leading to carcinogenic and neurological diseases. Therefore, the development of a selective, sensitive, and reliable OTA biodetection approach is crucial for ensuring food safety.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biotechnology & Applied Microbiology
Ana Isabel Mendes, Alexandra Gabriel Fraga, Maria Joao Peixoto, Ivo Aroso, Adhemar Longatto-Filho, Alexandra Pinto Marques, Jorge Pedrosa
Summary: Diabetic foot infection (DFI) is a significant cause of illness and death. Antibiotics are an essential treatment for DFI, but their effectiveness can be reduced due to bacterial biofilm formation and associated pathophysiology. Therefore, improved antibiotic therapies are needed to manage DFI more safely and effectively.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Engineering, Biomedical
Omar Alheib, Lucilia P. da Silva, Katia A. Mesquita, Alain da Silva Morais, Rogerio P. Pirraco, Rui L. Reis, Vitor M. Correlo
Summary: This study evaluated the potential of human adipose-derived mesenchymal stem cells (hASCs) pre-cultured in gellan gum based spongy-like hydrogels (SLHs). In vitro, hASCs were found to proliferate, release growth factors and cytokines important for muscular regeneration. In vivo, the SLHs with hASCs showed promoting effects on angiogenesis, neoinnervation, and the expression of myogenic proteins in a volumetric muscle loss (VML) mouse model.
BIOMEDICAL MATERIALS
(2023)
Correction
Cell & Tissue Engineering
Carla M. Abreu, Rogerio P. Pirraco, Rui L. Reis, Mariana T. Cerqueira, Alexandra P. Marques
STEM CELL RESEARCH & THERAPY
(2023)
Article
Materials Science, Biomaterials
Helena R. Moreira, Mariana T. Cerqueira, Lucilia P. da Silva, Joana Pires, Mariana Jarnalo, Ricardo Horta, Rui L. Reis, Alexandra P. Marques
Summary: Papillary and reticular fibroblasts in the skin have different functions and can promote capillary-like network formation, but their secretome and extracellular matrix (ECM) are substantially different.
BIOMATERIALS SCIENCE
(2023)
Article
Engineering, Biomedical
Ana Laura Martins de Andrade, Nivaldo Antonio Parizotto, Lucilia Pereira da Silva, Patricia Brassolatti, Richard Eloin Liebano, Alexandra Pinto Marques
Summary: This study analyzed the effect of different doses and energy levels of laser irradiation on hASCs cultured in 2D and 3D conditions. In 2D, metabolic activity and proliferation were enhanced only after multiple doses, while in 3D, cell proliferation increased after a single high-energy irradiation. More than 1 dose was required to promote ROS secretion, and a decrease in IL-8 secretion was observed only in 3D.
LASERS IN MEDICAL SCIENCE
(2023)
