Article
Engineering, Biomedical
Wentao Wang, Xiaolong Liang, Kai Zheng, Gaoran Ge, Xu Chen, Yaozeng Xu, Jiaxiang Bai, Guoqing Pan, Dechun Geng
Summary: Exosomes, small vesicles with the ability of vesicle-mediated intercellular signal transmission, have played an important role in bone tissue engineering. Compared to cells, exosomes have the advantage of cell-free therapy, which can promote bone regeneration and overcome immune rejection.
MATERIALS TODAY BIO
(2022)
Article
Biochemistry & Molecular Biology
Anna Majkowska, Karla E. Inostroza-Brito, Mariel Gonzalez, Carlos Redondo-Gomez, Alistair Rice, Jose Carlos Rodriguez-Cabello, Armando E. Del Rio Hernandez, Alvaro Mata
Summary: The study demonstrates the possibility to generate coassembling tubular membranes with distinct structures, properties, and bioactivity by minor modifications in the molecular structures of peptide amphiphiles and elastin-like recombinamers.
Article
Materials Science, Biomaterials
Jinsub Han, Sangbae Park, Jae Eun Kim, Byeongjoo Park, Yeonggeol Hong, Jae Woon Lim, Seung Jeong, Hyunmok Son, Hong Bae Kim, Hoon Seonwoo, Kyoung-Je Jang, Jong Hoon Chung
Summary: Developing an efficient and functional bone regeneration scaffold is challenging. A scaffold-on-a-chip device was developed to aid with the evaluation process. This device mimics the microenvironment experienced by a transplanted bone scaffold, allowing for monitoring of cell behavior at the blood-scaffold interphase.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Materials Science, Biomaterials
Jinsub Han, Sangbae Park, Jae Eun Kim, Byeongjoo Park, Yeonggeol Hong, Jae Woon Lim, Seung Jeong, Hyunmok Son, Hong Bae Kim, Hoon Seonwoo, Kyoung-Je Jang, Jong Hoon Chung
Summary: Developing an efficient and functional bone regeneration scaffold is challenging. A scaffold-on-a-chip device was developed as a platform for evaluation. The device mimics the microenvironment experienced by a transplanted bone scaffold and allows for monitoring of cell behavior. MC3T3-E1 cells were cultured with different scaffold inserts to evaluate their osteoconductivity and osteoinductivity. The results showed that the platform effectively evaluated the properties of the scaffolds. This proposed platform can replace animal models and contribute to the study of bone regeneration.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Engineering, Multidisciplinary
Wenmin Zhang, Caixia Liang, Qixuan He, Xiaoyan Cao, Fangli Yuan, Jiandong Ye
Summary: In this study, it was found that alumina ceramics with hydroxyapatite (HA) surface have better mineralization ability in a newly revised simulated body fluid, leading to increased deposition of HA crystals. These mineralized ceramics exhibit enhanced biological activity and osteogenesis properties in vitro. Increased formation of HA on the surface of alumina ceramics significantly promotes the ALP activity and expression of osteogenesis-related genes in bone marrow stromal stem cells.
JOURNAL OF BIONIC ENGINEERING
(2022)
Article
Materials Science, Multidisciplinary
Meng Luo, Konstantin Shaitan, Xiaoyan Qu, Anton P. Bonartsev, Bo Lei
Summary: Rare earth-based biomaterials possess representative optical/magnetic properties and special biological properties, which make them potential candidates for wound healing and skin tissue regeneration. Inorganic-organic hybrid rare earth-based biomaterials demonstrate promising applications in wound healing and repair.
APPLIED MATERIALS TODAY
(2022)
Review
Engineering, Biomedical
Adam Shearer, Maziar Montazerian, Jessica J. Sly, Robert G. Hill, John C. Mauro
Summary: At least 25 bioactive glass medical devices have been approved for clinical use worldwide. These devices have diverse applications, including implants, fillers, agents for dentin hypersensitivity, wound dressing, and cancer therapeutics. Bioactive glasses have evolved in morphology and delivery systems, allowing for greater flexibility and control. This article critically discusses the approved products, regulatory standards, and the future development, applications, and challenges of bioactive glasses.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Environmental
Meng Luo, Dagogo Dorothy Winston, Wen Niu, Yidan Wang, Hongyang Zhao, Xiaoyan Qu, Bo Lei
Summary: The study presented a multifunctional bioactive therapeutics-repair-enabled citrate-iron hydrogel scaffold that can inhibit tumor recurrence and promote wound repair simultaneously, showing great potential in clinical applications.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Physical
Silvia R. Gavinho, Ana Sofia Padua, Isabel Sa-Nogueira, Jorge C. C. Silva, Joao P. Borges, Luis C. C. Costa, Manuel Pedro F. Graca
Summary: Polymeric membranes are commonly used in guided bone regeneration (GBR) in dentistry. The addition of bioactive glasses with therapeutic ions, including zinc, can enhance cell adhesion, proliferation, and differentiation. In this study, bioactive glasses containing different concentrations of zinc were developed, and their structural and biological characteristics were evaluated. The results showed that the zinc-containing bioactive glasses did not exhibit significant antibacterial activity, but the highest concentration of zinc did not compromise bioactivity and promoted cell viability. Cell culture assays using the membranes demonstrated that the addition of zinc improved cell viability and increased alkaline phosphatase production.
Review
Biochemistry & Molecular Biology
Selvaraj Vimalraj, Rajamanikkam Yuvashree, Gopal Hariprabu, Raghunandhakumar Subramanian, Palraju Murali, Deepak Nallaswamy Veeraiyan, Lakshmi Thangavelu
Summary: Zebrafish serves as an excellent biomaterial testing platform for rapid characterization of the biological properties of new biomaterials, particularly in bone tissue engineering applications, surpassing technical difficulties and cost issues associated with rodent models.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Biomedical
Krzysztof Pietryga, Katarzyna Reczynska-Kolman, Janne E. Reseland, Havard Haugen, Veronique Larreta-Garde, Elzbieta Pamula
Summary: Biphasic monolithic materials were produced from polysaccharide hydrogel by enzymatic mineralization. The distribution of calcium phosphate mineral phase was achieved by limiting the availability of calcium glycerophosphate to specific parts of the hydrogel sample. The formation of CaP was confirmed by spectroscopy and mapping techniques.
BIOCYBERNETICS AND BIOMEDICAL ENGINEERING
(2023)
Article
Chemistry, Physical
Andrea Fiorati, Cristina Linciano, Camilla Galante, Maria Grazia Raucci, Lina Altomare
Summary: Cellulose is a low-cost, abundant, and renewable polysaccharide with a hierarchical structure composed of nanofibers. The oxidation of cellulose through TEMPO can result in nanofibers with improved properties for bone tissue regeneration. The addition of inorganic particles like CaP can enhance the mechanical properties and bioactivity of the resulting hydrogels.
Article
Green & Sustainable Science & Technology
Mano Govindharaj, Noura Sayed Al Hashemi, Soja Saghar Soman, Sanjairaj Vijayavenkataraman
Summary: This study successfully identified an environmentally destructive tunicate species and proposed sustainable utilization of tunicate tunics as wound-dressing materials, decellularized extracellular matrix (dECM) scaffolds, and bioinks. The tunics showed excellent biocompatibility and tensile modulus. The findings of this work contribute to the preservation of marine ecosystems and the reduction of costs associated with tunicate management.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Chemistry, Medicinal
Anita Kubiak, Martyna Pajewska-Szmyt, Martyna Kotula, Bartosz Lesniewski, Alona Voronkina, Parvaneh Rahimi, Sedigheh Falahi, Korbinian Heimler, Anika Rogoll, Carla Vogt, Alexander Ereskovsky, Paul Simon, Enrico Langer, Armin Springer, Maik Foerste, Alexandros Charitos, Yvonne Joseph, Teofil Jesionowski, Hermann Ehrlich
Summary: This study presents a new biomimetic approach for the development of a lepidocrocite-containing 3D spongin scaffold using artificial seawater and iron. The research proposes a mechanism of lepidocrocite formation and suggests the potential application of the biocomposite as an electrochemical dopamine sensor.
Article
Chemistry, Multidisciplinary
N. Alasvand, A. Behnamghader, P. B. Milan, M. Mozafari
Summary: This study successfully synthesized modified borate-based bioactive glasses with anticoagulant, antibacterial, and angiogenesis properties, showing great potential in blood-contact applications and vascular tissue engineering.
MATERIALS TODAY CHEMISTRY
(2023)
Article
Engineering, Biomedical
Maria Monteiro, Marta Rocha, Vitor M. Gaspar, Joao F. Mano
Summary: In this study, bioengineering pancreatic cancer models that mimic the tumor microenvironment using in vitro platforms was highly appreciated. These cancer-on-a-bead models exhibit biomimetic multicompartmentalization and tunable integration of cancer-associated stromal elements, recapitulating the signatures of human pancreatic tumors. The models also display increased drug resistance compared to their reductionistic counterparts.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Cell Biology
Daniela S. C. Bispo, Lenka Michalkova, Marlene Correia, Catarina S. H. Jesus, Iola F. Duarte, Brian J. Goodfellow, Mariana B. Oliveira, Joao F. Mano, Ana M. Gil
Summary: This study provides the first comprehensive characterization of the lipidome and exometabolome during osteogenic differentiation of human adipose tissue stem cells (hAMSCs) using NMR spectroscopy. Lipid changes primarily occur between days 1 and 7, followed by stabilization. The findings shed light on the mechanisms involved in membrane remodeling, antioxidant defense, protein synthesis, and energy metabolism during hAMSC osteogenic differentiation.
Article
Chemistry, Multidisciplinary
Raquel C. Goncalves, Sara Vilabril, Catarina M. S. S. Neves, Mara G. Freire, Joao A. P. Coutinho, Mariana B. Oliveira, Joao F. Mano
Summary: Compartmentalized structures obtained in all-aqueous settings have shown promising properties as cell encapsulation devices and reactors for trans-membrane chemical reactions. The interfacial stability conferred by emulsion systems enables the production of complex architectures. In this study, self-standing, malleable and perfusable tubular systems were fabricated through all-aqueous interfacial assembly, which can be used for cell culture and customized applications.
ADVANCED MATERIALS
(2022)
Article
Engineering, Biomedical
Maria V. Monteiro, Luis P. Ferreira, Marta Rocha, Vitor M. Gaspar, Joao F. Mano
Summary: This article presents the latest advances in bioengineering methods for in vitro modeling of the biological features and microenvironment of pancreatic cancer stroma, as well as providing design guidelines. The overview provides valuable examples and starting guidelines for researchers interested in engineering and characterizing stroma-rich biomimetic tumor models.
Article
Engineering, Biomedical
Sara Nadine, Ines Fernandes, Sonia G. Patricio, Clara R. Correia, Joao F. Mano
Summary: This study proposes a method to bioengineer biomimetic bone niches using immune cells, particularly macrophages. The incorporation of macrophages within the fabricated microcapsules allows for the recreation of an appropriate bone microenvironment and the development of new bone mineralized microtissues. The findings indicate the potential of using this method in bone tissue engineering and disease modeling.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Ana F. Cunha, Andre F. V. Matias, Cristovao S. Dias, Mariana B. Oliveira, Nuno A. M. Araujo, Joao F. Mano
Summary: Studying the interactions between living cells and mechanically stable materials helps us understand physiological phenomena and potentially develop innovative applications. By examining how cells interact with objects of different stability, we can uncover biological responses and address healthcare challenges.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Engineering, Biomedical
Catharina Husteden, Yazmin A. Brito Barrera, Sophia Tegtmeyer, Joao Borges, Julia Giselbrecht, Matthias Menzel, Andreas Langner, Joao F. Mano, Christian E. H. Schmelzer, Christian Woelk, Thomas Groth
Summary: This article presents a gene-activated surface coating strategy for designing smart biomaterials for bone tissue engineering. The thin-film coating is made of polyelectrolyte multilayers composed of collagen I and chondroitin sulfate, and further functionalized with DNA/lipid-nanoparticles. The successful formation of the thin-film, fibrillar structuring of collagen, and homogeneous embedding of lipoplexes is demonstrated. Culturing mesenchymal stem cells on the functionalized multilayer shows excellent attachment, growth, and uptake of cargo like fluorescence-labelled DNA from lipoplexes. Furthermore, the functionalization of the multilayer induces osteogenic differentiation of mesenchymal stem cells.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Materials Science, Multidisciplinary
Joao Rocha Maia, Rita Sobreiro-Almeida, Franck Cleymand, Joao F. Mano
Summary: Three-dimensional printing has emerged as a promising method for biofabrication of tissue engineering constructs, aiming to create tissue/organ replacements for precision medicine. By using human-sourced biomaterials with optimal rheological properties, the best possible scaffolds can be created, enhancing clinical outcomes. This paper provides an overview of current efforts in achieving necessary ink properties for biofabrication and discusses crosslinking strategies. It also explores the state-of-the-art of using human-sourced inks and presents future perspectives on the field.
JOURNAL OF PHYSICS-MATERIALS
(2023)
Review
Medicine, Research & Experimental
Daniela Patricio, Joana Santiago, Joao F. Mano, Margarida Fardilha
Summary: This article provides an overview of organoid technology and its applications in healthcare and research, with a focus on male reproductive system organoids and other biomaterials. The article also discusses current applications and highlights the research gaps, challenges, and opportunities in the field.
WIRES MECHANISMS OF DISEASE
(2023)
Article
Materials Science, Biomaterials
Adrian Hautmann, Devaki Kedilaya, Sanja Stojanovic, Milena Radenkovic, Christian K. Marx, Stevo Najman, Markus Pietzsch, Joao F. Mano, Thomas Groth
Summary: In this study, free-standing multilayer films (FSF) made of chitosan and alginate were crosslinked to achieve sustained release of growth factors and possessed biocompatibility and antibacterial properties. The films showed potential applications in wound healing.
BIOMATERIALS ADVANCES
(2022)
Meeting Abstract
Cell & Tissue Engineering
Sara Nadine, Clara R. Correia, Joao F. Mano
TISSUE ENGINEERING PART A
(2022)
Article
Polymer Science
Patricia Remuinan-Pose, Clara Lopez-Iglesias, Ana Iglesias-Mejuto, Joao F. Mano, Carlos A. Garcia-Gonzalez, M. Isabel Rial-Hermida
Summary: This study introduces alginate aerogel particles loaded with vancomycin obtained through a novel processing strategy combining gel inkjet printing and water-repellent surfaces, which can sustain release the antibiotic for more than 7 days. The green aerogel processing strategy significantly improves drug loading yields and has the potential to enhance antibacterial treatments for chronic wounds.
Article
Materials Science, Multidisciplinary
Dora C. S. Costa, Patricia D. C. Costa, Maria C. Gomes, Amit Chandrakar, Paul A. Wieringa, Lorenzo Moroni, Joao F. Mano
Summary: This study reports a versatile method to convert non-thermoresponsive hydrogels into thermoresponsive systems with shape memory ability. By embedding a thermoresponsive polyurethane mesh into different hydrogel networks, hydrogel composites with good shape fixity and recovery ability were achieved. This method has significant implications for conveying shape memory properties to hydrogels in various biological and nonbiological applications.
ACS MATERIALS LETTERS
(2022)
Review
Chemistry, Physical
Diana Jesus, Ana R. Pinho, Maria C. Gomes, Claudia S. Oliveira, Joao F. Mano
Summary: Bone tissue engineering aims to recreate the bone microenvironment for bone regeneration by guiding cell fate towards the osteogenic lineage. It is important to consider the synergistic effect of biochemical/physical cues, as cells in their native microenvironment are stimulated by multiple signals simultaneously. Therefore, combined modifications are crucial for developing innovative bone regenerative therapies.
Review
Materials Science, Biomaterials
Bruno M. Ladeira, Catarina A. Custodio, Joao F. Mano
Summary: Core-shell microcapsules have the potential to recreate the hierarchical structure of biological systems by independently modulating the composition of the core and shell layers. They can be tailored to recreate specific cell niches.
BIOMATERIALS SCIENCE
(2022)
