Article
Reproductive Biology
M. S. Ansari, B. A. Rakha, S. Akhter, A. Akhter, E. Blesbois, J. Santiago-Moreno
Summary: The study showed that using 0.5 mM GSH in the extender during cryopreservation of Indian red jungle fowl sperm can improve sperm quality and fertility by enhancing antioxidant potential and alleviating oxidative stress.
Article
Agriculture, Dairy & Animal Science
B. A. Rakha, M. S. Ansari, S. Akhter, A. Akhter, E. Blesbois
Summary: The study showed that intravaginal insemination at a depth of 4 cm enhances fertility outcomes of frozen-thawed Indian red jungle fowl semen compared to 2 cm depth.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shuai Wang, Hongjie Qiao, Pengxiang Wang, Yuan Wang, Danian Qin
Summary: Zdhhc19 knockout mice did not show disruption in spermatogenesis but exhibited male infertility with defects in sperm morphology and functions, highlighting the essential role of Zdhhc19 in sperm production and fertility in mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
M. Kanakachari, R. N. Chatterjee, M. R. Reddy, M. Dange, T. K. Bhattacharya
Summary: NGS sequencing was used to investigate the mitochondrial genomes of Indian native chickens and Red Jungle fowl, revealing 23 haplotypes and six significant clades. The study confirmed the Indian Red Jungle fowl as the origin of all Indian breeds.
FRONTIERS IN GENETICS
(2023)
Article
Reproductive Biology
Camilo Hernandez-Aviles, Luisa Ramirez-Agamez, Dickson D. Varner, Charles C. Love
Summary: The process of fertilization involves acrosomal exocytosis (AE) as the interaction between sperm and oocyte. While the role of sperm acrosome in fertilization has been extensively studied, there is limited knowledge about the molecular mechanisms governing acrosomal function in non-mice or human species. Evaluating sperm acrosomal function should be a routine test for individuals selected for breeding potential, even though acrosomal dysfunction-related subfertility is less common in large animals. High throughput technologies such as flow cytometry and mass spectrometry-based proteomic analysis can be used to study acrosomal function and discover new markers of acrosomal dysfunction in stallions.
Review
Cell Biology
Emma Linn, Lillian Ghanem, Hanisha Bhakta, Cory Greer, Matteo Avella
Summary: Spermatogenesis is a cell differentiation process crucial for the production of fertilizing sperm, which generally relies on Sertoli cells. Genetic factors play a key role in regulating spermatogenesis, and mutations in certain genes may lead to sperm functionality defects and rare genetic disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Haruhiko Miyata, Seiya Oura, Akane Morohoshi, Keisuke Shimada, Daisuke Mashiko, Yuki Oyama, Yuki Kaneda, Takafumi Matsumura, Ferheen Abbasi, Masahito Ikawa
Summary: SPATA33 interacts with sperm calcineurin via a PQIIIT sequence, and its knockout mice exhibit reduced sperm motility and impaired male fertility, which phenocopies Ppp3cc and Ppp3r2 knockout mice. Further analysis reveals that SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Bushra A. A. Rakha, Zartasha Zafar, Muhammad S. S. Ansari, Shamim Akhter, Saima Qadeer, Ali Akhter, Kainat Waseem, Julian Santiago-Moreno
Summary: The aim of this study was to investigate the effect of antibiotics on the sperm quality of Indian red jungle fowl, as well as to identify and isolate bacterial species and their antibiotic sensitivity. The results showed that the addition of penicillin to the semen extender improved semen quality. The bacteria isolated from semen were Escherichia coli, Staphylococcus spp., and Bacillus spp., with different sensitivity to antibiotics.
BIOPRESERVATION AND BIOBANKING
(2023)
Article
Obstetrics & Gynecology
Elisabetta Baldi, Meurig T. Gallagher, Stepan Krasnyak, Jackson Kirkman-Brown
Summary: The sixth edition of the World Health Organization manual explores extended examination methods that offer key diagnostics for investigating male reproductive system function. These methods go beyond basic semen analysis and can specifically guide the clinical characterization of fertile or infertile men. Multiparametric scoring for sperm morphological defects, sperm DNA fragmentation, and computer-assisted analysis of sperm or semen are among the most widely used and debated techniques.
FERTILITY AND STERILITY
(2022)
Article
Biotechnology & Applied Microbiology
Caroline Lindholm, Petros Batakis, Jordi Altimiras, John Lees
Summary: Research finds that intermittent fasting has significant effects on gene expression in the liver, particularly genes related to energy metabolism. Intermittent fasting promotes increased proliferative potential in both birds and mammals, indicating that the health benefits of periodic caloric restriction are not limited to mammals alone.
Review
Biochemistry & Molecular Biology
Cameron C. Gardner, Paul F. James
Summary: Na+/H+ exchangers (NHEs) are important regulators of pH in eukaryotic cells and play crucial roles in sperm function and male fertility. Mammalian sperm express multiple NHE isoforms that contribute to various aspects of sperm physiology and development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Miguel Ricardo Leung, Ravi Teja Ravi, Bart M. Gadella, Tzviya Zeev-Ben-Mordehai
Summary: Mammalian sperm undergo a complex process of capacitation in the female reproductive tract, culminating in acrosomal exocytosis. Cryoelectron tomography reveals the disassembly of paracrystalline structures within the acrosome during this process, supporting a mechanism for gradual dispersal of the acrosomal matrix. The unique membrane fission-fusion pathway in the sperm head maintains cell integrity while transforming the sperm for fertilization.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Akila Cooray, Jeongsook Kim, Beno Ramesh Nirujan, Nishani Jayanika Jayathilake, Kyu Pil Lee
Summary: Sperm ion channels play a crucial role in the quality and type of flagellar movement, with the principal potassium ion channel being responsible for membrane hyperpolarization and sperm capacitation. This study aimed to determine the channel identity of boar sperm for the primary K+ current. Pharmacological dissection was employed, and the results suggest that the Slo3 gene encodes the main K+ channel in boar spermatozoa. Localization of the Slo3 channel on the sperm flagellum and its inhibition not affecting sperm viability were observed. These findings have potential implications for improving assisted reproductive methods in industrial livestock production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dermatology
Burkhard T. Espey, Karin Kielwein, Hans van der Ven, Klaus Steger, Jean-Pierre Allam, Agnieszka Paradowska-Dogan, Katrin van der Ven
Summary: This study aimed to optimize the effects of PBM on human sperm, finding that exposure to laser energy doses of 4 and 6 J/cm(2) improved sperm motility and velocity in asthenozoospermic patients. Additionally, PBM showed no significant effect on DNA fragmentation level and CD46 expression serving as a biomarker for acrosome integrity.
LASERS IN SURGERY AND MEDICINE
(2022)
Article
Agriculture, Dairy & Animal Science
Won-Ki Pang, Ji-Hyun Son, Do-Yeal Ryu, Md Saidur Rahman, Yoo-Jin Park, Myung-Geol Pang
Summary: This study investigated the effects of differential mRNA expression of selected genes on fertilisation parameters in a porcine model. The results revealed a significant correlation between HSPD1 expression and male fertility, suggesting HSPD1 as a potential biomarker for superior boar selection in artificial insemination, ultimately boosting field fertility.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
B. Bernal, C. Castano, M. C. Esteso, A. Toledano-Diaz, M. A. Dominguez-Gonzalez, M. G. Gil, A. Lopez-Sebastian, J. L. Campo, E. Blesbois, J. Santiago-Moreno
Summary: The study found no significant differences in fresh semen variables between breeds and breeders, but significant differences were found in post-thawed variables. Cockerels from one of the breeders exhibited higher sperm cryoresistance, possibly due to differences in management practices.
BRITISH POULTRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
M. Marti-Colombas, M. J. Sanchez-Calabuig, C. Castano, A. Toledano-Diaz, E. Martinez-Nevado, A. Lopez-Goya, J. Santiago-Moreno
Summary: This study compared the effects of two cryoprotectants, DMA and DMSO, on the frozen-thawed sperm characteristics of black-footed penguins and gentoo penguins. The results showed that 8% DMSO was more effective for black-footed penguin sperm, while there were no significant differences in gentoo penguin samples. Additionally, black-footed penguin sperm was more sensitive to the freezing-thawing process compared to gentoo penguin sperm.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Cell Biology
Paula Boveda Gomez, Rosario Velazquez, Lucia Martinez-Fresneda, Octavio Mejia, Marta Oteo, Adolfo Toledano-Diaz, Cristina Castano, Milagros Cristina Esteso, Rodolfo Ungerfeld, Antonio Lopez-Sebastian, Julian Santiago-Moreno
Summary: This study examined the influence of induced changes in prolactin secretion on sperm cryoresistance in ibex and mouflon. The findings suggest that high levels of blood plasma prolactin negatively affect the cryoresistance of sperm in these animals.
BIOPRESERVATION AND BIOBANKING
(2023)
Article
Biology
H. Woelders, A. A. C. de Wit, B. Engel, B. Hulsegge, I. Grasseau, E. Blesbois, B. Bernal, J. Santiago-Moreno
Summary: This study modified a classical chicken semen diluent to have lowered osmolalities, allowing for cold storage and freezing of the semen with minimal loss of membrane integrity and motility. Different cryoprotective agents and cooling rates were tested to determine their effects on post-thaw sperm performance. The study found that specific combinations of cryoprotective agent concentration and cooling rate yielded the best results.
Article
Fisheries
Shaista Bibi, Rabea Ejaz, Muhammad Amjad Awan, Javeria Arshad, Bushra Allah Rakha, Muhammad Sajjad Ansari, Iqra Shakir, Shamim Akhter
Summary: This study evaluated the efficacy of different extenders and cryoprotectants for cryopreservation of Labeo rohita milt. The results showed that modified Kurokura-2 extender (KE) with methanol as cryoprotectant proved to be the best diluent, improving sperm motility and fertilization rate.
AQUACULTURE RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Rodolfo Ungerfeld, Julia Giriboni, Adolfo Toledano-Diaz, Madeleine Guerrero, Julian Santiago-Moreno
Summary: Transrectal ultrasonic-guided massage of the accessory sex glands (TUMASG) is a technique used for semen collection. Administering carbetocin before TUMASG can shorten the collection time and decrease electrical stimuli, but it has minimal effects on semen quality.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Reproductive Biology
C. M. Picazo, C. Castano, P. Boveda, A. Toledano-Diaz, R. Velazquez, B. Pequeno, M. C. Esteso, J. Gadea, S. Villaverde-Morcillo, J. Cerdeira, J. Santiago-Moreno
Summary: This study compared the effects of slow freezing and vitrification on different cell types in canine and wild boar testicular tissue. The results showed that vitrification had higher cell viability and DNA integrity in dogs, while either method could be used in wild boars.
Article
Reproductive Biology
Emma O'Brien, Clara Malo, Cristina Castano, Pedro Garcia-Casado, Adolfo Toledano-Diaz, Belen Martinez-Madrid, Heriberto Rodriguez-Martinez, Manuel Alvarez-Rodriguez, Julian Santiago-Moreno
Summary: This study investigated the presence and distribution of aquaglyceroporin 3 (AQP-3) in dromedary camel sperm, as well as its role in cryoinjury. The results showed that AQP-3 expression was not associated with the individual sperm response to freezing-thawing process. Additionally, sperm head morphometry was not a reliable predictor of cryosurvival.
Article
Veterinary Sciences
Julia Giriboni, Eva Martinez-Nevado, Juncal Garcia, Rosario Velazquez, Adolfo Toledano-Diaz, Rodolfo Ungerfeld, Julian Santiago-Moreno
Summary: Developing strategies for population control is crucial in both captive wildlife and production animals. Immunization against GnRH is an effective method for reduction of testosterone and sperm quality, but its effectiveness varies with species and treatment repetition.
Article
Veterinary Sciences
Patricia Peris-Frau, Julia Benito-Blanco, Eva Martinez-Nevado, Adolfo Toledano-Diaz, Cristina Castano, Rosario Velazquez, Belen Pequeno, Belen Martinez-Madrid, Milagros C. Esteso, Julian Santiago-Moreno
Summary: This study investigated the cryopreservation of testicular tissue using slow freezing and vitrification methods in diverse wild species. The results showed that the effectiveness of each method varied depending on the species and cell type.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Belen Pequeno, Cristina Castano, Manuel Alvarez-Rodriguez, Paula Boveda, Maria Gemma Millan de la Blanca, Adolfo Toledano-Diaz, Diego Andres Galarza, Heriberto Rodriguez-Martinez, Belen Martinez-Madrid, Julian Santiago-Moreno
Summary: The study aimed to investigate the influence of osmotic changes on the location of aquaporins (AQPs) in spermatozoa during freeze-thawing process. Individual variability in terms of freezeability was observed among rams and their ejaculates. AQP3 expression in the mid- and principal piece of spermatozoa was found to be higher in frozen-thawed samples compared to fresh specimens in the good freezability group, but such differences were not detected in the poor freezability group. AQP3 expression is associated with sperm cryotolerance and can be used as a biomarker.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Agriculture, Dairy & Animal Science
J. Santiago-Moreno, A. Toledano-Diaz, C. Castano, R. Velazquez, P. Boveda, E. O'Brien, P. Peris-Frau, B. Pequeno, B. Martinez-Madrid, M. C. Esteso
Summary: Reproductive technologies can help protect wild ruminant species from extinction and increase the number of animals. Efforts have focused on preserving sperm, oocytes, and embryos. Cryopreservation of sperm is the most commonly used and cheapest method for germplasm storage. New reproductive biotechnologies, such as ultra-rapid freezing techniques, have been developed. However, the success of artificial insemination using frozen-thawed sperm is not always satisfactory in wild small ruminants. Epididymal sperm shows higher cryoresistance than ejaculated sperm, and the seasonal variation in testosterone and prolactin levels is involved in sperm cryoresistance.
Article
Biology
Adrian Martin, Cristina Castano, Emma O'Brien, Adolfo Toledano-Diaz, Rafael Guerra, Felix Gomez-Guillamon, Julian Santiago-Moreno
Summary: This study examines the effect of equilibration time with extender on the variables of ultra-rapidly frozen-thawed sperm from wild ruminants. The results show that equilibration improves the quality and viability of sperm. Epididymal sperm demonstrates greater cryoresistance, while ejaculated sperm shows no dimensional changes before and after equilibration.
Article
Agriculture, Dairy & Animal Science
O. U. Garcia-Cruz, L. M. Tejada, M. J. Flores, L. E. Nava-Rivera, N. Lopez-Magana, H. Hernandez, M. Keller, P. Chemineau, J. Santiago-Moreno, J. A. Delgadillo
Summary: The results of this study indicate that male goats in a semi-extensive management system have lower plasma testosterone concentrations, sexual behavior, and sperm quantitative and qualitative production than those in a confined management system.
ANIMAL PRODUCTION SCIENCE
(2022)
Article
Developmental Biology
B. Bernal, A. Behnamifar, C. Alvarez-Rodriguez, A. Toledano-Diaz, C. Castano, R. Velazquez, M. G. Gil, A. Gutierrez-Adan, Henri Woelders, E. Blesbois, J. Santiago-Moreno
Summary: This study aimed to evaluate the chromatin compaction of rooster spermatozoa along the male reproductive tract and to study the vas deferens lining cells potentially involved in sperm maturation. The results showed that the transit though the vas deferens resulted in a higher percentage of compacted chromatin spermatozoa in the ejaculate.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2022)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.
