Article
Cell Biology
Atsuko Kusuhara, Elnur Babayev, Luhan T. Zhou, Vijay P. Singh, Jennifer L. Gerton, Francesca E. Duncan
Summary: The study found that egg quality parameters in mice improve during the pubertal transition, but eggs in the early pubertal period exhibit chromosome configuration abnormalities, indicating a cell cycle delay and originating from more immature follicles.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Obstetrics & Gynecology
Marta Venturas, Xingbo Yang, Kishlay Kumar, Dagan Wells, Catherine Racowsky, Daniel J. Needleman
Summary: Fluorescence lifetime imaging microscopy (FLIM) can accurately measure the metabolic state of cumulus cell clusters, with parameters significantly correlated with patient age and AMH levels but not with BMI. This technique may have potential value in identifying superior oocytes.
FERTILITY AND STERILITY
(2021)
Article
Reproductive Biology
Monica De los Reyes, Jaime Palomino, Allison Villagra, George Ramirez, Oscar A. Peralta, Victor H. Parraguez, Karla Aspee
Summary: This study aimed to evaluate the effect of progesterone on meiotic development related to the gene expression of connexin 37 and connexin 43 in canine cumulus oocyte complexes. The results showed that progesterone increased meiotic development in canine oocytes but had no effect on the gene expression of connexin 37 and connexin 43.
Article
Reproductive Biology
Maria Florencia Suqueli Garcia, Nadia Gabbanelli, Glenda Laura Rios, Jorgelina Buschiazzo
Summary: This study found that progesterone (P4) can induce acrosome exocytosis in sperm without compromising sperm viability or activating sperm caspases. Furthermore, using lower concentrations of exogenous P4 during in vitro fertilization (IVF) can enhance the developmental competence of partially cumulus-denuded oocytes.
Article
Biochemistry & Molecular Biology
Ralf Poehland, Jens Vanselow, Fabiana Melo Sterza
Summary: This study investigated the presence of HES1 in both compartments of bovine cumulus oocyte complexes (COCs) and its distribution after in vitro maturation (IVM). HES1 mRNA was detectable in oocytes but not in cumulus cells, while the protein was mainly visible in cumulus cells. GFP-HES1-mRNA injection showed signal in both oocytes and cumulus cells, suggesting protein transfer from oocytes to cumulus cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Ishani Dinisri, Sandarenu Kodikara, Madhusha Prasadani, Indunil Pathirana, Chathura Rathnayake, Basil Alexander, Kai-Fai Lee, Suranga Pradeep Kodithuwakku
Summary: Mancozeb negatively impacts caprine oocytes and granulosa cells, potentially impairing fertilization ability and developmental potential of the oocytes, and suppressing ovulation. It suppresses granulosa cells viability, reduces cumulus expansion, and suppresses metaphase plate formation in oocytes, while also inducing progesterone secretion from granulosa cells.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2021)
Article
Biology
Jimena Garcia-Rodriguez, Amanda Ferreira Cunha, Adriana Morales-Guerrero, Adrian Gonzalez-Chaves, Agustin Camacho, Lucilia Souza Miranda, Filipe C. Serrano, Adrian Jaimes-Becerra, Antonio Carlos Marques
Summary: Medusozoans exhibit diverse life cycles, with different semaphoronts representing the sexual phase. Our comprehensive review found that egg size, although phylogenetically conserved within medusa classes, is influenced by reproductive and environmental traits such as the number of eggs, depth, and temperature. Additionally, egg size variation among cold-water species of Staurozoa may be related to the development of small benthic larvae in this group.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Reproductive Biology
Linah Al-Alem, Muraly Puttabyatappa, Ketan Shrestha, Yohan Choi, Kathy Rosewell, Mats Brannstrom, James Akin, Misung Jo, Diane M. Duffy, Thomas E. Curry
Summary: The study found that NTS expression in the ovaries of humans and rats significantly increased during ovulation, possibly regulated by the epidermal growth factor pathway and protein kinase A pathway. NTS may regulate crucial aspects of ovulation such as vascular permeability, inflammation, and cell migration.
BIOLOGY OF REPRODUCTION
(2021)
Article
Obstetrics & Gynecology
Eva M. E. Balkenende, Taghride Dahhan, Catharina C. M. Beerendonk, Kathrin Fleischer, Dominic Stoop, Annelies M. E. Bos, Cornelis B. Lambalk, Roel Schats, Jesper M. J. Smeenk, Leonie A. Louwe, Astrid E. P. Cantineau, Jan Peter de Bruin, Sabine C. Linn, Fulco van der Veen, Madelon van Wely, Mariette Goddijn
Summary: Alternative ovarian stimulation protocols with tamoxifen or letrozole did not affect the number of cumulus-oocyte complexes (COCs) retrieved at follicle aspiration in women with breast cancer.
HUMAN REPRODUCTION
(2022)
Article
Developmental Biology
Kaitlin R. Karl, Peter Z. Schall, Zaramasina L. Clark, Meghan L. Ruebel, Jose Cibelli, Robert J. Tempelman, Keith E. Latham, James J. Ireland
Summary: Excessive FSH doses during ovarian stimulation in heifers with small ovarian reserve can cause disruptions in cumulus cell and oocyte functions, leading to premature cumulus expansion and follicular hyperstimulation dysgenesis. These effects can impair the quality of cumulus-oocyte complexes and hinder successful fertilization.
MOLECULAR HUMAN REPRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Jun-Xue Jin, Jing-Tao Sun, Chao-Qian Jiang, Hong-Di Cui, Ya Bian, Sanghoon Lee, Lianjin Zhang, Byeong Chun Lee, Zhong-Hua Liu
Summary: This study reveals the role of melatonin receptors in lipid metabolism and redox signaling during in vitro cumulus-oocyte complex (COC) development, highlighting their potential to improve female reproductive health. Understanding the dynamics of interactions between lipid homeostasis and redox signaling driven by melatonin receptors is crucial for predicting drug targets and therapeutic effects.
Article
Agriculture, Dairy & Animal Science
Lee D. Spate, Jie Yin, Lauren Sammel, Randall S. Prather, Bethany K. Redel
Summary: COVID-19 has had a significant impact on abattoirs worldwide, resulting in a critical loss of pig material for research. Commercial sources of pig oocytes are expensive and already operating at maximum capacity. This study presents an alternative source of oocytes that can produce live, healthy piglets.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Agriculture, Multidisciplinary
Jiaxin Duan, Huali Chen, Yuan Li, Dejun Xu, Xiaoya Li, Zelin Zhang, Jianyong Cheng, Li Yang, Qingwang Li
Summary: This study identified the significant role of estrogen in regulating GJICs in porcine COCs, promoting nuclear maturation through upregulating autophagy. The process is regulated by the MEK/ERK signaling pathway.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Reproductive Biology
Laura Falchi, Ivo Pivato, Mauro Ledda, Valentino Melosu, Antonio Scanu, Salvatore Pau, Sergio Ledda, Maria Teresa Zedda
Summary: Intra-follicular oocyte transfer (IFOT) has shown promise in equines and bovines, and this study has demonstrated its feasibility in ovine species. However, further optimization is needed to improve embryo recovery rates in sheep.
Review
Cell Biology
Batara Sirait, Budi Wiweko, Ahmad Aulia Jusuf, Dein Iftitah, R. Muharam
Summary: This study examines several biomarkers in the cumulus-oocyte complex for their predictive significance in evaluating oocyte developmental competence and IVF outcomes. While these biomarkers show potential for predicting oocyte competency, challenges limit their practical application in IVF as prognostic tools.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
L. M. G. Paim, L. L. Gal, R. F. F. Lopes, A. T. D. Oliveira
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2015)
Article
Agriculture, Dairy & Animal Science
E. F. Lopes, L. S. Marques, R. G. Duranti, A. T. D. de Oliveira, R. F. F. Lopes, J. L. Rodrigues
REPRODUCTION IN DOMESTIC ANIMALS
(2015)
Article
Rheumatology
N. Glesse, P. Vianna, L. M. G. Paim, M. C. C. Matte, A. K. K. Aguiar, P. L. Palhano, O. A. Monticielo, C. V. Brenol, R. M. Xavier, J. A. B. Chies
Article
Multidisciplinary Sciences
Pol Grasland-Mongrain, Ali Zorgani, Shoma Nakagawa, Simon Bernard, Lia Gomes Paim, Greg Fitzharris, Stefan Catheline, Guy Cloutier
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Toxicology
Roberto Marques Damiani, Marcella Ody Piva, Marcelo Rafael Petry, Paulo Hilario Nascimento Saldiva, Alexandre Tavares Duarte de Oliveira, Claudia Ramos Rhoden
TOXICOLOGY MECHANISMS AND METHODS
(2012)
Article
Cell Biology
Diego Duarte Alcoba, Bianca Leticia da Rosa Braga, Nathallie Louise Sandi-Monroy, Leticia Auler Proenca, Rui Fernando Felix Lopes, Alexandre Tavares Duarte de Oliveira
Article
Biochemistry & Molecular Biology
Cayetana Vazquez-Diez, Lia Mara Gomes Paim, Greg FitzHarris
Article
Multidisciplinary Sciences
Lia Mara Gomes Paim, Greg FitzHarris
NATURE COMMUNICATIONS
(2019)
Editorial Material
Developmental Biology
Lia Mara Gomes Paim, Greg FitzHarris
Article
Multidisciplinary Sciences
Lia Mara Gomes Paim, Greg FitzHarris
Summary: This study investigates the dynamics of furrow ingression in early mouse embryos during cell division. It finds that cell size is an important determinant of furrow ingression speed, and the emergence of cell polarity and assembly of the apical domain negatively regulate furrow ingression on one side. The study reveals that cell polarity directly regulates the recruitment of cytokinetic machinery in a cell-autonomous manner.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Agriculture, Dairy & Animal Science
L. A. Ritt, E. C. Modesto, J. A. Guimara, G. Heisler, A. T. D. Oliveira, V. Fischer
Summary: The aim of this study was to evaluate the effects of oregano extract supplementation on the gastrointestinal tract of suckling dairy calves. It was found that oregano extract increased the empty weight of the abomasum, but did not affect the weight of other segments. Calves supplemented with oregano extract had lower number of rumen branches and papillae/branches. However, most histological variables were not affected by oregano extract supplementation.
Article
Cell Biology
Iaskara Oliveira, Joana Fisch, Juliana Gomes, Rui Fernando Felix Lopes, Alexandre Tavares Duarte de Oliveira
Summary: The objective of this study was to determine the cleavage and embryo developmental rates after parthenogenetic activation of cumulus-oocyte complexes (COCs) selected by different concentrations of brilliant cresyl blue (BCB) and cryopreservation. The results showed that the 13 μM BCB group displayed greater development rates at the morula stage, and the control group of fresh COCs had the highest cleavage and morula development rates. Comparing morula rates between vitrified COC control and BCB(+) groups, the BCB(+) group had better results. Therefore, BCB staining selection can effectively improve cryopreservation outcomes.
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.