Journal
TALANTA
Volume 167, Issue -, Pages 462-469Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2017.02.048
Keywords
Choline biosensor; Choline oxidase; Multiwalled carbon nanotubes; Gold nanoparticles; Electrochemical impedance spectroscopy
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Funding
- European Commission [294993]
- Fundacao para a Ciencia e a Tecnologia (FCT), Portugal [PTDC/QEQ-QAN/2201/2014, 3599-PPCDT]
- European Community Fund FEDER [UID/EMS/00285/2013]
- FCT [SFRH/BPD/103103/2014]
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A novel amperometric biosensor for choline determination has been developed, exploiting the electrocatalytic properties of multiwalled carbon nanotubes (MWCNT) and gold nanoparticles (GNP). Chitosan (Chit), a natural biocompatible polymer, was used to disperse CNT, then Chit-MWCNT was dropped on the surface of a glassy carbon electrode (GCE), followed by GNP; finally, choline oxidase (ChOx) was immobilized by glutaraldehyde crosslinking. The ChOx/(GNP)(4)/MWCNT/GCE exhibited linear response to choline from 3 to 120 mu M, the sensitivity was 204 mu A cm(-2) mM(-1) and the detection limit was 0.6 mu M. The biosensor exhibited good intra and inter-electrode precision, and excellent selectivity and stability. Electrochemical impedance spectroscopy (EIS) was also used to measure choline at 0.0 V and this is the first report on choline determination by EIS. Successful measurement in milk samples was performed.
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