Article
Biochemistry & Molecular Biology
Ivan Fan Xia, Hang-Kin Kong, Margaret M. H. Wu, Yishan Lu, Ka-Hing Wong, Kevin W. H. Kwok
Summary: The study identified that selenium nanoparticles (SeNPs) exhibit strong immunomodulatory and antioxidant activities, closely related to lipid metabolism. The enhanced biological functions by SeNPs are nearly identical in healthy and disease conditions, but SeNP may promote ROS formation during disease states.
Article
Chemistry, Multidisciplinary
Weiwei Tang, Jun-Jie Shi, Wei Liu, Xu Lu, Bin Li
Summary: A matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) assisted genome mining strategy was applied to discover a glycosyltransferase (GT) named PgGT1 from the root of Platycodon grandiflorum. PgGT1 was found to catalyze the synthesis of platycoside E (PE) by sequentially attaching two beta-1,6-linked glucosyl residues to the glucosyl residue at the C3 position of platycodin D (PD). The study also revealed the crucial roles of residues S273, E274, and H350 in stabilizing the sugar donor and positioning the glucose for optimal glycosylation. These findings provide insights into the biosynthetic pathway of PE and have potential implications in industrial biotransformation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Biochemical Research Methods
Jessica L. Moore, Georgia Charkoftaki
Summary: This review discusses recent advances in imaging mass spectrometry, specifically matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) applied on tissues. It explores sample preparation strategies, considerations for different analyte classes, and the use of multimodal imaging strategies. The review also examines experimental design approaches, standardization of processes, and data analysis platforms. It concludes with applications of imaging mass spectrometry in various fields, with a focus on medical research.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Nhu Q. Vu, Amanda R. Buchberger, Jillian Johnson, Lingjun Li
Summary: Neuropeptides are low abundance signaling molecules that modulate physiological processes, and mass spectrometry imaging is increasingly useful in studying them. Proper tissue washes can increase detectable neuropeptides, and using a salt solution wash may affect neuropeptide detection.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Spectroscopy
Colin T. McDowell, Xiaowei Lu, Anand S. Mehta, Peggi M. Angel, Richard R. Drake
Summary: This article introduces the development of glycan imaging mass spectrometry and discusses its applications in different sample types. Glycan imaging mass spectrometry analyses broaden our understanding of the biological and clinical relevance of N-glycosylation to human disease.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Qiuqin Zhou, Stefano Rizzo, Janina Oetjen, Annabelle Fueloep, Miriam Rittner, Hartmut Gillandt, Carsten Hopf
Summary: Insufficient vacuum stability of matrix chemicals is a major limitation in MALDI mass spectrometry imaging of large tissue sample cohorts. A photo-cleavable caged molecule, DMNB-2,5-DHAP, was designed and synthesized for lipid MALDI-MSI of mouse brain tissue sections. DMNB-2,5-DHAP exhibited vacuum stability in a high vacuum MALDI ion source and its uncaging process resulted in the generation of 2,5-DHAP with the assistance of the built-in laser in the MALDI ion source.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Mandana Hunter, Nicholas J. Demarais, Richard L. M. Faull, Angus C. Grey, Maurice A. Curtis
Summary: Huntington's disease (HD) is a fatal disorder with no effective interventions. Research indicates lipidomic abnormalities in HD patients, with variations in the abundance of certain lipid species. These findings support further investigation into the potential role of lipidomic aberrations in HD.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Biochemical Research Methods
Caitlin Tressler, Sloane Tilley, Ethan Yang, Christopher Donohue, Eric Barton, Alain Creissen, Kristine Glunde
Summary: This study optimized matrix spraying parameters for mouse whole kidney lipid imaging using automated pneumatic spraying and a heated sample-holder tray, resulting in improved MALDI imaging performance. The use of sample heating allowed for higher solvent flow rates for better sample extraction, while minimizing analyte delocalization.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Environmental Sciences
Yanyan Chen, Lilong Jiang, Rong Zhang, Zhangsheng Shi, Chengyi Xie, Yanjun Hong, Jianing Wang, Zongwei Cai
Summary: This study used AP-MALDI MSI to reveal the spatial distributions of PFOS in mouse kidneys and its adverse effects on lipid metabolism. PFOS was found to accumulate mainly in the renal pelvis and outer cortex regions, causing damage to the kidneys. Significant down-regulation of certain lipids and up-regulation of others were observed in different regions of the kidneys between control and PFOS-exposed mice.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Analytical
Taiga Iwama, Kuniyuki Kano, Daisuke Saigusa, Kim Ekroos, Gerhild van Echten-Deckert, Johannes Vogt, Junken Aoki
Summary: The novel on-tissue derivatization method using Phos-tag in combination with MALDI-MSI enables visualization of low-abundance bioactive lipids like LPA and S1P in the brain, providing a simple and optimal way to reveal the spatial localization of these important molecules in tissues.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Saleh M. Khalil, Richard R. Sprenger, Martin Hermansson, Christer S. Ejsing
Summary: Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is a useful technique for visualizing the spatial distribution of lipid molecules in tissues. The limitation of confident lipid identification in MSI has been overcome by the development of DDA-imaging technology. This study demonstrates the successful transfer of DDA-imaging technology onto another mass spectrometer and shows its reliability in lipid identification.
JOURNAL OF MASS SPECTROMETRY
(2022)
Review
Chemistry, Physical
Paulina Kret, Anna Bodzon-Kulakowska, Anna Drabik, Joanna Ner-Kluza, Piotr Suder, Marek Smoluch
Summary: The science related to biomaterials and tissue engineering is expanding, with a focus on surface modifications and interactions with surrounding tissues. Mass Spectrometry Imaging (MSI) techniques can measure molecular composition from biomaterials and adjacent tissue simultaneously, without the need for antibodies or dyes that may affect the results.
Article
Chemistry, Multidisciplinary
Congnan Peng, Qian Zhang, Jian-an Liu, Zhen-peng Wang, Zhen-wen Zhao, Ning Kang, Yuxin Chen, Qing Huo
Summary: The study explored a methodology using TiO2 NPs as a matrix for lipid determination with MALDI MS. It found that TiO2 NPs are preferable for determining lipid standards and mixed lipids, and could be a potential direction for future research on matrix development.
GREEN PROCESSING AND SYNTHESIS
(2021)
Article
Biochemical Research Methods
Lianlian Ma, Qingrong Xie, Mingyi Du, Yudi Huang, Yingying Chen, Dong Chen, Yizhu Xu, Hanhong Xu, Xinzhou Wu, Zhibin Yin
Summary: This study systematically investigated the optimized sample preparation conditions for insects and model organisms in mass spectrometry imaging (MSI), providing guidelines for MSI analysis and expanding its potential in life sciences and environmental sciences.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Ethan Yang, Jeong Hee Kim, Caitlin M. Tressler, Xinyi Elaine Shen, Dalton R. Brown, Cole C. Johnson, Tae -Hun Hahm, Ishan Barman, Kristine Glunde
Summary: Multimodal tissue imaging techniques, such as RSI and MALDI MSI, have the potential to provide spatially resolved, sensitive, specific biomolecular information for unraveling biological processes and identifying disease biomarkers. The development of RaMALDI, a streamlined, integrated workflow, allows for the combined imaging of RSI and MALDI MSI on a single tissue section with one sample preparation protocol, effectively integrating molecular information and driving discoveries in various fields.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemical Research Methods
Sabrina Trog, Ahmed H. El-Khatib, Sebastian Beck, Marcus R. Makowski, Norbert Jakubowski, Michael W. Linscheid
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Medicinal
Hesham El-Askary, Heba Handoussa, Farid Badria, Ahmed H. El-Khatib, Abdulrhman Alsayari, Michael W. Linscheid, Amira Abdel Motaal
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY
(2019)
Article
Biochemical Research Methods
Stefanie Ickert, Timm Schwaar, Andreas Springer, Marko Grabarics, Jens Riedel, Sebastian Beck, Kevin Pagel, Michael W. Linscheid
JOURNAL OF MASS SPECTROMETRY
(2019)
Article
Pharmacology & Pharmacy
G. Artiaga, A. Iglesias-Jimenez, E. Moreno-Gordaliza, M. L. Mena, M. M. Gomez-Gomez
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2019)
Article
Food Science & Technology
Reham Wagdy, Reham M. Abdelkader, Ahmed H. El-Khatib, Michael W. Linscheid, Nabila Hamdi, Heba Handoussa
JOURNAL OF FOOD SCIENCE
(2019)
Article
Cardiac & Cardiovascular Systems
Jacob Amersfoort, Frank H. Schaftenaar, Hidde Douna, Peter J. van Santbrink, Gijs H. M. van Puijvelde, Bram Slutter, Amanda C. Foks, Amy Harms, Estefania Moreno-Gordaliza, Yanyan Wang, Thomas Hankemeier, Ilze Bot, Hongbo Chi, Johan Kuiper
Summary: The study found that dyslipidemia affects the migration of Treg cells, leading to inadequate immunosuppression in atherosclerotic lesions. This condition alters the migration capacity and cellular metabolism of Treg cells, increasing their migration to lymphoid tissues and influencing infiltration into atherosclerotic lesions.
CARDIOVASCULAR RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Juan Carlos Jado, Blanca Humanes, Maria Angeles Gonzalez-Nicolas, Sonia Camano, Jose Manuel Lara, Beatriz Lopez, Emilia Cercenado, Julio Garcia-Bordas, Alberto Tejedor, Alberto Lazaro
Article
Biochemistry & Molecular Biology
Matilde Zaballos, Mercedes Power, Maria Iluminada Canal-Alonso, Maria Angeles Gonzalez-Nicolas, Wenceslao Vasquez-Jimenez, Pablo Lozano-Lominchar, Pilar Cabrerizo-Torrente, Natividad Palencia-Garcia, Susana Gago-Quiroga, Maria Dolores Ginel-Feito, Consuelo Jimenez, Alberto Lazaro, Luis Gonzalez-Bayon
Summary: Preventive use of imipenem/cilastatin can reduce the nephrotoxicity of cisplatin in patients with peritoneal carcinomatosis, leading to lower creatinine values and shorter ICU and hospital stay in the postoperative period.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Estefania Moreno-Gordaliza, Maria Dolores Marazuela, Oscar Pastor, Alberto Lazaro, Maria Milagros Gomez-Gomez
Summary: Nephrotoxicity is a common complication of cisplatin-based chemotherapy, but there is currently no preventive intervention or treatment available for clinical use. Cilastatin has shown to have a nephroprotective effect in in vitro and in vivo models and is now in clinical trials. Understanding the molecular mechanisms of cisplatin-induced renal damage and the effects of potential protective agents could lead to successful nephroprotective therapies and new biomarkers of renal damage and protection. A targeted lipidomics approach was used to quantify lipid species in kidney extracts from rats treated with cisplatin and/or cilastatin, showing significant changes in lipid levels after cisplatin treatment which were attenuated by co-treatment with cilastatin. Cholesterol esters, along with sulfatides and phospholipids, were found to be the most discriminating lipid species in differentiating renal damage from nephroprotection. Additionally, potential diagnostic biomarkers of cisplatin-induced renal damage and cilastatin nephroprotection were identified.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Rebeca Gonzalez-Fernandez, Maria Angeles Gonzalez-Nicolas, Manuel Morales, Julio Avila, Alberto Lazaro, Pablo Martin-Vasallo
Summary: The study suggests that FKBP51, AmotL2, and IQGAP1 are overexpressed in various types of cancer, with the highest increase observed in leucocytes from patients undergoing oxaliplatin chemotherapy. Microscopic examination of renal tissue indicates these proteins may play a role in the development of cisplatin toxicity in kidney tubules.
Article
Food Science & Technology
M. D. Marazuela, M. Klaiber, E. Moreno-Gordaliza, A. Barata, M. M. Gomez-Gomez
Summary: This study found that TCS migration was observed in commercial antibacterial food containers despite the EU ban on TCS. Moreover, microplastics were found to leach from the surface of these containers, possibly leading to the transfer of TCS into food and posing a potential health risk to consumers.
FOOD PACKAGING AND SHELF LIFE
(2022)
Article
Microbiology
Johanna Bensalel, Alexandra Roberts, Kiara Hernandez, Angelica Pina, Winifred Prempeh, Blessing V. Babalola, Pablo Cannata, Alberto Lazaro, Julio Gallego-Delgado
Summary: The impact of malaria-associated acute kidney injury (MAKI) has been underestimated and neglected in research, leading to a lack of standard mouse model for studying this pathology. This study developed an in vivo model in wild-type mice by performing unilateral nephrectomies prior to infection with Plasmodium berghei NK65. The nephrectomized mice showed kidney injury and elevated levels of acute kidney injury (AKI) biomarkers compared to non-nephrectomized mice. This model is critical for understanding the molecular pathways, disease development, biomarkers, and potential therapies for MAKI.
Article
Chemistry, Applied
Estefania Moreno-Gordaliza, M. Dolores Marazuela, M. Milagros Gomez-Gomez
Summary: The evaluation of migration of ionic silver and nanoparticulated silver from antimicrobial plastic packaging to food is essential for ensuring its safety. Migration assays were conducted on reusable silver-containing polypropylene (PP) food containers and a silicone baby bottle using food simulants, under different conditions. The PP containers released significant amounts of silver, while migration in the silicone bottle was much lower. Risk assessment showed that some containers exceeded the safety levels recommended by European authorities. No significant release of AgNPs was detected, but silver-containing microplastics and silicone microparticles were found in the food simulants. Continuous exposure to ionic silver and microplastics could potentially lead to health issues.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
