Journal
TALANTA
Volume 167, Issue -, Pages 359-366Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2017.02.039
Keywords
Metal-organic framework; Glycine modification; Enhanced peroxidase-like activity; Glucose; Biosensing
Categories
Funding
- National Natural Science Foundation of China [51678485]
- Fundamental Research Funds for the Central Universities [XDJK2016D019]
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A facile and rapid post-synthetic strategy was proposed to prepare a glycine functionalized MIL-53(Fe), namely glycine-MIL-53(Fe), by a simple mixing of water dispersible MIL-53(Fe) and glycine. The FT-IR, SEM, XRD and zeta potential were used to characterize the glycine-MIL-53(Fe). The result showed that glycine post-synthetic modification of MIL-53(Fe) did not change in the morphology and crystal structure of MIL-53(Fe). Interestingly, compared with MIL-53(Fe), the glycine-MIL-53(Fe) exhibits an enhanced peroxidase-like activity, which could catalyze the oxidation of TMB by H2O2 to produce an intensive color reaction. Kinetic analysis indicated that the K-m of glycine-MIL-53(Fe) for TMB was one-tenth of that of MIL-53(Fe). The glycine-MIL-53(Fe) as peroxidase mimetic displays better stability under alkaline or acidic conditions than MIL-53(Fe). The good performance of glycine-MIL-53(Fe) over MIL-53(Fe) may be attributed to the increase of affinity between TMB and the glycine-MIL-53(Fe). With these characteristics, a simple and sensitive method was developed for the detection of H2O2 and glucose. The linear detection range for H2O2 is 0.10-10 mu M with a detection limit of 49 nM, and glucose could be linearly detected in the range from 0.25 to 10 mu M with a detection limit of 0.13 mu M. The proposed method was successfully used for glucose detection in human serum samples.
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