Article
Biochemistry & Molecular Biology
Martyna Smeda, Kerstin M. Galler, Melanie Woelflick, Andreas Rosendahl, Christoph Moehle, Beate Lenhardt, Wolfgang Buchalla, Matthias Widbiller
Summary: Both dental pulp and apical papilla are potential sources of stem cells for regenerative endodontic treatments. This study compared the molecular characteristics of dental pulp stem cells (DPSC) and stem cells from the apical papilla (SCAP). The results showed that both cell types expressed similar mesenchymal stem cell markers and had the ability to differentiate into various lineages. However, differences were observed in the profiles of secreted molecules and gene regulation. Overall, both DPSC and SCAP are suitable for dental pulp tissue engineering from a molecular biological perspective.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Aalaa E. Eldessoky, Mohammed M. Khalefa, Ashraf M. Abu-Seida
Summary: This study compared the effectiveness of 980 nm diode laser and double antibiotic paste in regenerative endodontic therapy for mature teeth with necrotic pulp and apical periodontitis in a dog model. The results showed that 980 nm diode laser can be an alternative to double antibiotic paste for root canal disinfection, leading to accelerated regenerative endodontic therapy.
Article
Dentistry, Oral Surgery & Medicine
Ana Flavia Balestrero Cassiano, Heman Coaguila-Llerena, Cintia Silva Santos, Luana Raphael da Silva, Lucas Fabricio Bahia Nogueira, Pietro Ciancaglini, Gisele Faria
Summary: This study assessed the effects of OCT on stem cells from human dental pulp and apical papilla. The results showed that OCT promoted migration, proliferation, and alkaline phosphatase activity, which could be advantageous for regenerative endodontic procedures.
JOURNAL OF ENDODONTICS
(2022)
Review
Biochemistry & Molecular Biology
Qi Liu, Yuan Gao, Jinzhi He
Summary: Dental diseases can cause premature tooth root development arrest in young permanent teeth, and sustained root elongation is crucial for long-term preservation. Stem cell-based regenerative endodontic treatment using stem cells from the apical papilla (SCAPs) has emerged as a promising strategy for treating pulp and periapical infections in young permanent teeth. This review summarizes the advances in SCAPs studies and discusses the challenges that still need to be addressed.
Review
Dentistry, Oral Surgery & Medicine
Mohammad Sabeti, Daniel Ghobrial, Maryam Zanjir, Bruno R. da Costa, Yating Young, Amir Azarpazhooh
Summary: This study aimed to assess the most successful treatment modality for regenerative endodontic treatment (RET) in immature permanent teeth with pulpal necrosis. The results of network meta-analysis showed that blood clot scaffold, platelet-rich plasma (PRP), and platelet-rich fibrin (PRF) exhibited good treatment effects, but their long-term efficacy is limited.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Zafer C. Cehreli, Gizem Erbas Unverdi, Elif Ballikaya
Summary: This case series demonstrates the clinical and radiographic outcomes of regenerative endodontic treatment (RET) using deciduous pulp autotransplantation in traumatized, necrotic young permanent incisors. Follow-up observations at 12 and 24 months showed radiographic evidence of complete periapical healing, slight increase in dentinal wall thickness, and continued apical closure in all patients.
JOURNAL OF ENDODONTICS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Diana B. Sequeira, Ana Rafaela Oliveira, Catarina M. Seabra, Paulo J. Palma, Carlos Ramos, Maria H. Figueiredo, Ana C. Santos, Ana L. Cardoso, Joao Peca, Joao Miguel Santos
Summary: The findings of this study demonstrate that SCAPs combined with PRP scaffold can induce the formation of dentin-like and pulp-like tissues in root segments. The presence of barriers such as ProRoot MTA or Biodentine did not significantly affect the deposition of hard material in the root segments, but the area of newly deposited dentin was significantly greater in segments with a Biodentine barrier compared to a ProRoot MTA barrier.
CLINICAL ORAL INVESTIGATIONS
(2021)
Review
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper assesses the hormetic dose responses of stem cells from the apical papilla in humans and provides insights into their underlying mechanisms and potential therapeutic applications.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Review
Engineering, Multidisciplinary
Naresh Kumar, Nazrah Maher, Faiza Amin, Hani Ghabbani, Muhammad Sohail Zafar, Francisco Javier Rodriguez-Lozano, Ricardo E. Onate-Sanchez
Summary: This paper thoroughly reviews and discusses various pulp-regenerative approaches and materials used in regenerative endodontics, highlighting current trends and future research prospects in this field.
Article
Dentistry, Oral Surgery & Medicine
Pierre-Olivier Miron, Amel Ben Lagha, Jabrane Azelmat, Anibal Diogenes, Juliana Nascimento Santos, Daniel Grenier
Summary: The study found that TNF- has detrimental effects on the viability, proliferation rate, and mineralization potential of stem cells of the apical papilla. Additionally, treating SCAP with TNF- reduced the secretion of periostin and increased the secretion of various cytokines and MMPs.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Seung-Kyung Kwon, Minjae Kyeong, Dinuka Adasooriya, Sung-Won Cho, Il-Young Jung
Summary: This case report describes the histologic and electron microscopic characteristics of a patient who underwent regenerative endodontic procedure for a chronic apical abscess. The regenerated root tissue exhibited a structure similar to the native root structure, indicating the regenerative potential of cell-free regenerative endodontic procedures for teeth with pulp necrosis and chronic apical abscess.
JOURNAL OF ENDODONTICS
(2023)
Article
Cell Biology
Suphalak Phothichailert, Nunthawan Nowwarote, Benjamin P. J. Fournier, Vorapat Trachoo, Sittiruk Roytrakul, Worachat Namangkalakul, Thanaphum Osathanon
Summary: This study investigated the characteristics of Jagged1-treated dental pulp stem cell-derived decellularized extracellular matrix (dECM) and its impact on the odonto/osteogenic differentiation of stem cells isolated from apical papilla (SCAPs). The results showed that Jagged1-treated dECMs were biocompatible and enhanced the odonto/osteogenic differentiation of SCAPs, suggesting their potential use in regenerative medicine as ECM scaffolds.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Napas Meeprasert, Jeeraphat Jantarat, Wassana Wichai, Rudee Surarit, Kenneth M. Hargreaves
Summary: This study evaluated the effect of EDTA and saline as the final irrigation in regenerative endodontic procedures on the attachment, proliferation, migration, and differentiation of stem cells from the apical papilla. The results showed that irrigating dentin with EDTA followed by a final irrigation with 20 mL saline promoted the proliferation of stem cells without affecting their differentiation.
CLINICAL ORAL INVESTIGATIONS
(2023)
Article
Engineering, Biomedical
Qian Li, Zhiai Hu, Yongxi Liang, Cancan Xu, Yi Hong, Xiaohua Liu
Summary: The study developed a multifunctional peptide-conjugated, pH-sensitive non-viral gene vector for fast revascularization and pulp regeneration in a full-length human tooth root. This vector significantly increased gene transfection efficiency, reduced cytotoxicity, upregulated VEGF expression in dental pulp stem cells, and accelerated new blood vessel formation and pulp tissue regeneration. The approach proves to be safe and effective for regenerative endodontics.
ACTA BIOMATERIALIA
(2021)
Article
Dentistry, Oral Surgery & Medicine
Fang-Chi Li, Alireza Shahin-Shamsabadi, P. Ravi Selvaganapathy, Anil Kishen
Summary: This study developed and characterized a 3D tissue construct for studying the interactions between SCAPs and macrophages in proinflammatory and anti-inflammatory environments. The results showed distinct interactions between SCAPs and macrophages in the tissue constructs, and the growth of cells and cell-to-cell and cell-to-matrix interactions resulted in a change in the tissue graft's dimensions.
JOURNAL OF ENDODONTICS
(2022)