Article
Multidisciplinary Sciences
Yin-Yu Lam, Chun-Ho Chan, Lin Geng, Nicodemus Wong, Wendy Keung, Yiu-Fai Cheung
Summary: Cardiomyocytes can be derived from hiPSC lines, but the efficacy varies. By performing single cell transcriptomics, researchers identified cardiac and extra-cardiac lineages during differentiation. They found APLNR as a surface marker for in vitro cardiac progenitors, and using this marker, they isolated and differentiated cardiac progenitors from multiple hiPSC lines. This study has potential applications in studying cardiac development, disease modeling, and cardiac regeneration.
Article
Cell & Tissue Engineering
Katherine Minter-Dykhouse, Timothy J. Nelson, Clifford D. L. Folmes
Summary: During the differentiation process of human-induced pluripotent stem cells (hiPSCs) into cardiomyocytes (CMs), the regulation of cell cycle differs from in vivo mouse cardiac development, resulting in premature exit of hiPSC-CMs from the cell cycle despite their relative immaturity.
STEM CELLS AND DEVELOPMENT
(2022)
Article
Biochemistry & Molecular Biology
Martha E. Floy, Sophie E. Givens, Oriane B. Matthys, Taylor D. Mateyka, Charles M. Kerr, Alexandra B. Steinberg, Ana C. Silva, Jianhua Zhang, Ying Mei, Brenda M. Ogle, Todd C. McDevitt, Timothy J. Kamp, Sean P. Palecek
Summary: The study explores the developmental lineage and functional properties of cardiac fibroblasts derived from human pluripotent stem cells, highlighting their potential in modeling fibrotic diseases and regenerative therapies in the context of heart function. The differences in characteristics between epicardial and second heart field cardiac fibroblasts provide insights for in vitro model selection and therapeutic applications.
Article
Biochemistry & Molecular Biology
Lauren Michelle Walker, Nicole R. L. Sparks, Veronica Puig-Sanvicens, Beatriz Rodrigues, Nicole zur Nieden
Summary: This study updated the classic mouse EST with endpoints derived from human induced pluripotent stem cells (hiPSCs) and human fibroblasts, showing improved prediction of human risks; the hiPSC-EST was able to detect inhibition of differentiation at lower concentrations and adverse developmental outcomes caused by novel environmental toxicants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Markus Krane, Martina Dressen, Gianluca Santamaria, Ilaria My, Christine M. Schneider, Tatjana Dorn, Svenja Laue, Elisa Mastantuono, Riccardo Berutti, Hilansi Rawat, Ralf Gilsbach, Pedro Schneider, Harald Lahm, Sascha Schwarz, Stefanie A. Doppler, Sharon Paige, Nazan Puluca, Sophia Doll, Irina Neb, Thomas Brade, Zhong Zhang, Claudia Abou-Ajram, Bernd Northoff, Lesca M. Holdt, Stefanie Sudhop, Makoto Sahara, Alexander Goedel, Andreas Dendorfer, Fleur V. Y. Tjong, Maria E. Rijlaarsdam, Julie Cleuziou, Nora Lang, Christian Kupatt, Connie Bezzina, Rudiger Lange, Neil E. Bowles, Matthias Mann, Bruce D. Gelb, Lia Crotti, Lutz Hein, Thomas Meitinger, Sean Wu, Daniel Sinnecker, Peter J. Gruber, Karl-Ludwig Laugwitz, Alessandra Moretti
Summary: The study revealed disruptions in gene programs and cellular processes in patients with hypoplastic left heart syndrome (HLHS), particularly in key aspects of fetal ventricular cardiogenesis such as the cell cycle and cardiomyocyte maturation. These abnormalities result in defects in early differentiation of cardiac progenitors, leading to left ventricular hypoplasia.
Article
Cardiac & Cardiovascular Systems
Shiqiao Ye, Cankun Wang, Zhaohui Xu, Hui Lin, Xiaoping Wan, Yang Yu, Subhodip Adhicary, Joe Z. Zhang, Yang Zhou, Chun Liu, Matthew Alonzo, Jianli Bi, Angelina Ramirez-Navarro, Isabelle Deschenes, Qin Ma, Vidu Garg, Joseph C. Wu, Ming-Tao Zhao
Summary: This study demonstrates that NOTCH1 is crucial for the differentiation and proliferation of human cardiac cells. The absence of NOTCH1 leads to defective ventricular-like cardiomyocyte differentiation and impaired cell proliferation. These findings shed light on the mechanisms underlying hypoplastic left heart syndrome.
CIRCULATION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Sunday Ndoma Bobori, Yuxiang Zhu, Alicia Saarinen, Alexis Josephine Liuzzo, Clifford D. L. Folmes
Summary: Growing evidence suggests that metabolites and energy metabolism have an active role in regulating cellular fate, particularly in cardiac development. A study on human pluripotent stem cells (PSCs) reveals significant metabolic remodeling during cardiac lineage specification, with most changes occurring during transitions from pluripotency to mesoderm and mesoderm to early cardiac stages. The findings highlight the opportunity to optimize metabolism during differentiation to support the specification and maturation of human PSC-derived cardiomyocytes.
Article
Ecology
Lewis Revely, Seirian Sumner, Paul Eggleton
Summary: This paper discusses the importance of termites as a model for studying developmental plasticity and proposes a conceptual framework for describing the phenotypic plasticity of termites. Currently, there is limited research on hemimetabolous social insects, with a bias towards holometabolous social insects in the literature. The proposed framework could have wider relevance for studying other hemimetabolous insects and holometabolous social insects with high caste polyphenism.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2021)
Article
Biology
Jianhua Zhang, Zachery R. Gregorich, Ran Tao, Gina C. Kim, Pratik A. Lalit, Juliana L. Carvalho, Yogananda Markandeya, Deane F. Mosher, Sean P. Palecek, Timothy J. Kamp, Gordana Vunjak-Novakovic
Summary: This study reveals the importance of extracellular matrix (ECM) proteins, specifically fibronectin (FN), in promoting cardiac differentiation of human pluripotent stem cells (hPSCs). The presence of FN in the ECM is essential for effective cardiac differentiation, while other ECM proteins like laminin-111 and laminin-521 inhibit this process. Inhibition of certain integrin receptors and integrin-linked kinase can also impair cardiac differentiation.
Review
Pharmacology & Pharmacy
Michelle Vanessa Kapchoup Kamga, Michael Reppel, Jurgen Hescheler, Filomain Nguemo
Summary: Long QT syndrome, Brugada syndrome, and catecholaminergic polymorphic ventricular tachycardia are genetic heart diseases caused by mutations in cardiac ion channels, leading to paroxysmal arrhythmias with different clinical manifestations and functional perturbations. Abnormal sodium and calcium signaling plays a crucial role in these diseases. Patient-specific in vitro models based on induced pluripotent stem cells can help address the underlying mechanisms and develop personalized therapies.
BIOCHEMICAL PHARMACOLOGY
(2021)
Review
Cardiac & Cardiovascular Systems
Aditya K. Birla, Sunita Brimmer, Walker D. Short, Oluyinka O. Olutoye, Jason A. Shar, Suriya Lalwani, Philippe Sucosky, Anitha Parthiban, Sundeep G. Keswani, Christopher A. Caldarone, Ravi K. Birla
Summary: Hypoplastic left heart syndrome is a complex congenital heart condition that requires surgical management for survival. Understanding the genetic basis and exploring innovative therapies such as stem cell therapy and tissue engineering are important for further advancements in treating this condition.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Eleanor Luce, Clara Steichen, Soumeya Abed, Anne Weber, Philippe Leboulch, Leila Maouche-Chretien, Anne Dubart-Kupperschmitt
Summary: The study demonstrates that siPSCs can differentiate into liver cells and self-organize into 3D structures, facilitating research on liver development and the development of co-culture systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Pediatrics
Rebecca Abraham, Luca Vricella, Narutoshi Hibino
Summary: Hypoplastic left heart syndrome (HLHS) is a deadly congenital heart disease that can be treated with stem cell-based regenerative therapies. These therapies have shown promise in improving heart function in HLHS patients undergoing palliative surgery. However, further development and research are needed, particularly in the generation of larger and more complex cardiac tissue patches for robust and long-lasting heart rebuilding. Overall, stem cell-based therapies have great potential in improving the treatment of HLHS.
TRANSLATIONAL PEDIATRICS
(2023)
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Review
Pharmacology & Pharmacy
Anthony T. Bejjani, Neil Wary, Mingxia Gu
Summary: Hypoplastic left heart syndrome (HLHS) is a severe developmental defect characterized by underdevelopment of the left ventricle, requiring multiple palliative surgeries for survival. Genetic variants associated with HLHS include transcription factors, chromatin remodelers, structural proteins, and signaling proteins needed for normal heart development. Current clinical trials have shown promising results at improving cardiac function in patients who have undergone palliative surgeries.
EXPERT OPINION ON THERAPEUTIC TARGETS
(2021)
Article
Cardiac & Cardiovascular Systems
David Adlam, Timothy M. Olson, Nicolas Combaret, Jason C. Kovacic, Siiri E. Iismaa, Abtehale Al-Hussaini, Megan M. O'Byrne, Sara Bouajila, Adrien Georges, Ketan Mishra, Peter S. Braund, Valentina d'Escamard, Siying Huang, Marios Margaritis, Christopher P. Nelson, Mariza de Andrade, Daniella Kadian-Dodov, Catherine A. Welch, Stephani Mazurkiewicz, Xavier Jeunemaitre, Claire Mei Yi Wong, Eleni Giannoulatou, Michael Sweeting, David Muller, Alice Wood, Lucy McGrath-Cadell, Diane Fatkin, Sally L. Dunwoodie, Richard Harvey, Cameron Holloway, Jean-Philippe Empana, Xavier Jouven, Jeffrey W. Olin, Rajiv Gulati, Marysia S. Tweet, Sharonne N. Hayes, Nilesh J. Samani, Robert M. Graham, Pascal Motreff, Nabila Bouatia-Naji
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2019)
Article
Cardiac & Cardiovascular Systems
Wei Zhou, J. Martijn Bos, Dan Ye, David J. Tester, Sybil Hrstka, Joseph J. Maleszewski, Steve R. Ommen, Rick A. Nishimura, Hartzell Schaff, Chang Sung Kim, Michael J. Ackerman
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2019)
Article
Cardiac & Cardiovascular Systems
Harold M. Burkhart, Muhammad Yasir Qureshi, Joseph W. Rossano, Susana Cantero Peral, Patrick W. O'Leary, Matthew Hathcock, Walter Kremers, Timothy J. Nelson, Amanda Breuer, Karen Cavanaugh, Frank Cetta, Joseph A. Dearani, Allan Dietz, Brooks Edwards, Shauna Hirsch, Kimberly Holst, Karen Krucker, Kathryn Lenn, Sara Martineau, Christopher E. Mascio, Angela Majerus, Angela Miller, Jennifer Miller, Karen Miller, Arshid Mir, Timothy M. Olson, Darci Radel, Chelsea Reece, Lori Riess, Sameh M. Said, Juanita Taylor, Julia Thebiay, Jess L. Thompson, Mark Wentworth, Joan Wobig
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2019)
Article
Pediatrics
Rabia Javed, Frank Cetta, Sameh M. Said, Timothy M. Olson, Patrick W. O'Leary, Muhammad Yasir Qureshi
PEDIATRICS IN REVIEW
(2019)
Article
Cardiac & Cardiovascular Systems
Tamiel N. Turley, Jeanne L. Theis, Rhianna S. Sundsbak, Jared M. Evans, Megan M. O'Byrne, Rajiv Gulati, Marysia S. Tweet, Sharonne N. Hayes, Timothy M. Olson
CIRCULATION-GENOMIC AND PRECISION MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Analyne M. Schroeder, Massoud Allahyari, Georg Vogler, Maria A. Missinato, Tanja Nielsen, Michael S. Yu, Jeanne L. Theis, Lars A. Larsen, Preeya Goyal, Jill A. Rosenfeld, Timothy J. Nelson, Timothy M. Olson, Alexandre R. Colas, Paul Grossfeld, Rolf Bodmer
HUMAN MOLECULAR GENETICS
(2019)
Article
Cardiac & Cardiovascular Systems
Tamiel N. Turley, Megan M. O'Byrne, Matthew L. Kosel, Mariza de Andrade, Rajiv Gulati, Sharonne N. Hayes, Marysia S. Tweet, Timothy M. Olson
Article
Biology
Jeanne L. Theis, Georg Vogler, Maria A. Missinato, Xing Li, Tanja Nielsen, Xin-Xin I. Zeng, Almudena Martinez-Fernandez, Stanley M. Walls, Anais Kervadec, James N. Kezos, Katja Birker, Jared M. Evans, Megan M. O'Byrne, Zachary C. Fogarty, Andre Terzic, Paul Grossfeld, Karen Ocorr, Timothy J. Nelson, Timothy M. Olson, Alexandre R. Colas, Rolf Bodmer
Article
Neurosciences
Sybil C. L. Hrstka, Soneela Ankam, Busranur Agac, Jon P. Klein, Raymond A. Moore, Bhavya Narapureddy, Isabella Schneider, Ronald F. Hrstka, Surendra Dasari, Nathan P. Staff
Summary: This study identified proteomic changes in human induced pluripotent stem cell-derived sensory neurons exposed to bortezomib, with significant impact on microtubule dynamics, cytoskeletal organization, and molecular transport. Pathway analysis revealed enrichment in unfolded protein response and integrated stress response signaling pathways, while alterations in MAP2 suggested its influential role in bortezomib-induced neurotoxicity.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Jeanne L. Theis, Jessie J. Hu, Rhianna S. Sundsbak, Jared M. Evans, William R. Bamlet, M. Yasir Qureshi, Patrick W. O'Leary, Timothy M. Olson
Summary: This study identified defects in cardiomyopathy-associated genes in patients with HLHS through whole genome sequencing, indicating potential impact on the functional reserve of the single-ventricle circulation.
CIRCULATION-GENOMIC AND PRECISION MEDICINE
(2021)
Article
Cell & Tissue Engineering
Ashley A. Krull, Deborah O. Setter, Tania F. Gendron, Sybil C. L. Hrstka, Michael J. Polzin, Joseph Hart, Amel Dudakovic, Nicolas N. Madigan, Allan B. Dietz, Anthony J. Windebank, Andre J. van Wijnen, Nathan P. Staff
Summary: Our study showed that MSCs maintained their morphology but became quiescent under intrathecal-like conditions. Transcriptomic analysis revealed a distinct gene expression profile for MSCs cultured in artificial CSF, with induction of genes related to angiogenesis and immunomodulation. Additionally, we found a dose-dependent increase in growth factors and immunomodulatory cytokines in CSF from ALS patients treated intrathecally with autologous MSCs, suggesting therapeutic potential.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cardiac & Cardiovascular Systems
Jeanne L. Theis, Talha Niaz, Rhianna S. Sundsbak, Zachary C. Fogarty, William R. Bamlet, Donald J. Hagler, Timothy M. Olson
Summary: By performing whole-genome sequencing and filtering for rare, predicted-damaging variants, the study identified the significant role of CELSR1 gene in familial BAV and HLHS, further implicating the importance of planar cell polarity pathway perturbation in congenital heart disease.
CIRCULATION-GENOMIC AND PRECISION MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Jeanne L. Theis, Sarah-Dana H. Shatila, Zachary C. Fogarty, William R. Bamlet, Timothy M. Olson
Summary: By analyzing whole genome sequences, this study identified candidate genes for HLHS using three independent strategies. The results showed that variants in the MYO18B, MYO5A, and MYO15A genes are associated with HLHS. These findings provide further insights into HLHS pathogenesis.
CIRCULATION-GENOMIC AND PRECISION MEDICINE
(2023)
Article
Oncology
Nathan P. Staff, Sybil C. Hrstka, Surendra Dasari, Enrico Capobianco, Sandra Rieger
Summary: This study investigated the effects of paclitaxel therapy on the skin of breast cancer patients with chemotherapy-induced peripheral neuropathy (CIPN). Neurological examinations, quality of life questionnaires, and skin punch biopsies were conducted, revealing no significant difference in intraepidermal nerve fiber (IENF) density between control and CIPN patients. However, RNA sequencing and immunostaining showed upregulated MMP-13 expression and ultrastructural changes in collagen and basement membrane structures.
Article
Biochemistry & Molecular Biology
Jay W. Schneider, Saji Oommen, Muhammad Y. Qureshi, Sean C. Goetsch, David R. Pease, Rhianna S. Sundsbak, Wei Guo, Mingming Sun, Han Sun, Hidehito Kuroyanagi, Dennis A. Webster, Alexander W. Coutts, Kimberly A. Holst, Brooks S. Edwards, Nikolas Newville, Matthew A. Hathcock, Tamene Melkamu, Francesca Briganti, Wu Wei, Maria G. Romanelli, Scott C. Fahrenkrug, Doug E. Frantz, Timothy M. Olson, Lars M. Steinmetz, Daniel F. Carlson, Timothy J. Nelson