Article
Chemistry, Analytical
Hao Chen, Yuefei Zhang, Haichao Zhou, Weiran Chen, Jiayi Peng, Yang Feng, Linyuan Fan, Jun Li, Jin Zi, Yan Ren, Qidan Li, Siqi Liu
Summary: Spatial proteomics plays an important role in the era of single-cell biology. In this study, a new routine workflow was developed for proteomic analysis of micro-FFPE tissues, allowing the construction of a spatial proteomic atlas of mouse brain, which exhibited typical characteristics of spatial-dependent protein abundance and functional enrichment.
ANALYTICAL CHEMISTRY
(2023)
Article
Spectroscopy
Ivo Krempl, Karel Novotny, Vojtech Wertich, Radek Skoda, Viktor Kanicky, Jaromir Leichmann
Summary: The aim of this work is to demonstrate the potential of LIBS as a complementary technique to EPMA for distinguishing and characterizing uranium mineralizations. Combining both methods can help estimate uranium oxidation states and monitor the possible mobilization of uranium in the environment by detecting oxygen and hydrogen using LIBS. The study confirms that the LIBS signal of oxygen is proportional to oxygen content and that the strength of the oxygen signal is closely related to the oxidation state of uranium.
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
(2023)
Article
Biochemistry & Molecular Biology
Peter M. Abuja, Daniela Pabst, Benjamin Bourgeois, Martina Loibner, Christine Ulz, Iris Kufferath, Ulrike Fackelmann, Cornelia Stumptner, Rainer Kraemer, Tobias Madl, Kurt Zatloukal
Summary: Molecular diagnostics in healthcare is increasingly dependent on high-quality nucleic acids obtained from appropriately preserved tissue specimens. This study establishes a method for measuring residual water in fixed paraffin-embedded tissue and demonstrates its correlation with the performance of nucleic acids in qRT-PCR analysis. Improving the shelf life of fixed tissue requires meticulous dehydration and dry storage to minimize the degradative influence of residual water on nucleic acids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Ouafa Sijilmassi, Jose Manuel Lopez-Alonso, Aurora Del Rio Sevilla, Maria del Carmen Barrio Asensio
Summary: The article presents a biomedical diagnostic technique based on polarized light detection to visualize structural damage in paraffin-embedded tissues, aiding in the diagnosis of tissue abnormalities, particularly in cases where dietary folic acid deficiency affects developing embryos. Through this method, changes in tissues can be visualized and identified, allowing for comparison with a healthy control group.
JOURNAL OF BIOPHOTONICS
(2021)
Article
Spectroscopy
Cecile Fabre, Kimberly Trebus, Alexandre Tarantola, Jean Cauzid, Vincent Motto-Ros, Panagiotis Voudouris
Summary: This article introduces analytical methods for determining the distribution of elements in geological samples and discusses the advantages of mu LIBS and mu XRF techniques. By applying these methods to a mica schist thin section, the accuracy of major and trace element imaging is demonstrated, as well as the ability to differentiate minerals in more complex rock matrices.
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
(2022)
Article
Multidisciplinary Sciences
Sabine Bauer, Jessica Pauli, Jaroslav Pelisek
Summary: Extraction of DNA from formalin-fixed tissues poses challenges due to degradation, but an automated system established in the study shows promise in obtaining high-quality DNA for downstream analyses, especially for small FFPE tissue specimens.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Oncology
Saeedesadat Badieyan, Mitra Abedini, Mohammadreza Razzaghi, Afshin Moradi, Mohammadreza Masjedi
Summary: This paper investigates the application of polarimetry imaging technique in pathological diagnosis of bladder tissues. By measuring the optical polarization properties of bulk and Formalin-Fixed Paraffin-Embedded (FFPE) bladder tissues, and applying Mueller matrix polar decomposition and transformation methods for analysis, it is found that the extracted parameters can be used to differentiate the microstructural differences between normal and cancerous tissues. The method can be used for in vivo optical biopsy and has the potential to significantly shorten the duration of pathological diagnosis.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2023)
Article
Spectroscopy
Marketa Hola, Karel Novotny, Jan Dobes, Ivo Krempl, Vojtech Wertich, Juraj Mozola, Martin Kubes, Veronika Faltusova, Jaromir Leichmann, Viktor Kanicky
Summary: The study demonstrated the great potential of combining LIBS and LA-ICP-MS imaging techniques, providing advantages of high speed, large area imaging, high resolution, and low detection limits. A special software, ILAPS, was created to ensure data processing consistency, effectively identifying structures responsible for elements migration in uranium ore samples.
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
(2021)
Article
Developmental Biology
Rachel M. Coyte, Thomas H. Darrah, Emily Barrett, Thomas G. O'Connor, John W. Olesik, Carolyn M. Salafia, Ruchit Shah, Tanzy Love, Richard K. Miller
Summary: There is growing interest in measuring metals concentrations in human placentas for various purposes, but the comparison between formalin-fixed paraffin embedded (FFPE) tissues and frozen tissues has been limited. In this study, paired samples of frozen and FFPE tissue from 22 human placentae were analyzed for metal concentrations, and the results showed no significant differences between the two types of tissue preparations.
Article
Chemistry, Analytical
Tian Ning, Heping Li, Yishen Chen, Baoping Zhang, Furong Zhang, Shuang Wang
Summary: The application of Raman microspectroscopy in histopathological analysis is widely pursued for characterizing ex vivo biological tissue. With the wider applicability of dewaxed formalin-fixed paraffin-embedded (FFPE) samples in hospitals, an abundant tissue bank is a useful resource for retrospective research to improve diagnosis accuracy. By comparing fresh frozen and FFPE breast tissue samples, the dewaxing process significantly altered the biochemical composition of tissues, but multivariate analysis methods could still effectively distinguish tissue types.
VIBRATIONAL SPECTROSCOPY
(2021)
Article
Multidisciplinary Sciences
Jing Chang, Xiang Li, Qingxin Xia, Shumin Yang, He Zhang, Hui Yang
Summary: Microbial communities found in breast cancer tissues may contribute to oncogenesis. Analysis of FFPE and FF tumor samples from 30 BC participants revealed consistent microbial composition and stable core bacterial communities, indicating the potential value of FFPE tissues for intratumoral microbiome analysis in breast cancer.
Review
Oncology
Amin Talebi, Jean Paul Thiery, Mohammad Amin Kerachian
Summary: Chimeric transcripts are important for diagnosis and prognosis, but their identification from FFPE tissues is limited due to the reduced quality and quantity of RNA. Strategies need to be developed to overcome these limitations and maximize the potential of FFPE tissues as a source for fusion transcript identification.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2021)
Article
Pathology
Sonia Paytubi, Miren Taberna, Marisa Mena, Beatriz Quiros, Montserrat Goma, Laia Alemany, Francesc X. Bosch, Miguel A. Pavon
Summary: HPV-related OPSCCs represent a distinct clinical entity with unique treatment response and survival outcomes. The study evaluated the AmpFire Multiplex HR-HPV tests for detection and genotyping of HPV in OPSCC FFPE samples, showing high overall agreement with Linear Array HPV-DNA Genotyping Test. This study demonstrates that AmpFire HPV Tests are simple and effective for HPV-DNA detection in OPSCC FFPE samples, suitable for clinical practice implementation.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2022)
Article
Chemistry, Analytical
Rory T. Steven, Marcel Niehaus, Adam J. Taylor, Ammar Nasif, Efstathios Elia, Richard J. A. Goodwin, Zoltan Takats, Josephine Bunch
Summary: Mass spectrometry imaging (MSI) is a powerful technique for providing spatially resolved atomic and molecular information from almost any sample type. Optimizing MSI methods for analysis of FFPE tissue is necessary due to the prevalence and importance of FFPE tissue storage.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Raphael Legouffe, Olivier Jeanneton, Mathieu Gaudin, Aurore Tomezyk, Amandine Gerstenberg, Marc Dumas, Catherine Heusele, David Bonnel, Jonathan Stauber, Sylvianne Schnebert
Summary: Hyaluronic acid plays a vital role in the skin, and its reduction with age has led to widespread use of HA-containing products in cosmetics and aesthetic procedures. A new analytical method using MALDI-MSI has been developed to quantify HA content in the skin and compare relative concentrations in different tissue areas. The technique has shown a significant increase in HA levels following topical application of HA formula, demonstrating its potential efficacy in combating signs of aging.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Dermatology
Pauline Pralong, Meryl Ciszynski, Marie Moncourier, Jean-Claude Beani, Julie Charles, Marie-Therese Leccia
Summary: This study confirms the usefulness of phototesting in diagnosing photodermatoses, with polymorphous light eruption (PLE) being the most common type, followed by photocontact dermatitis (PCD) and solar urticaria. Due to the potential severity of photodermatoses which may require hospitalization, maintaining expertise in this area is critical.
PHOTODERMATOLOGY PHOTOIMMUNOLOGY & PHOTOMEDICINE
(2022)
Article
Biophysics
Marie Beylot-Barry, Emmanuel Mahe, Carole Rolland, Maud Amy de la Breteque, Claire Eychenne, Julie Charles, Catherine Payen, Laurent Machet, Celine Vermorel, Alison Foote, Christian Roques, Jean-Luc Bosson
Summary: This multicenter study demonstrates that spa therapy can improve the quality of life of psoriasis patients and alleviate certain symptoms in the short and long term.
INTERNATIONAL JOURNAL OF BIOMETEOROLOGY
(2022)
Article
Biochemical Research Methods
Benoit Busser, Anne-Laure Bulin, Vincent Gardette, Helene Elleaume, Frederic Pelascini, Alexandre Bouron, Vincent Motto-Ros, Lucie Sancey
Summary: In this study, the application of Laser-Induced Breakdown Spectroscopy (LIBS) in imaging the distribution of chemical elements in brain tissues is explored.
JOURNAL OF NEUROSCIENCE METHODS
(2022)
Retraction
Chemistry, Multidisciplinary
Sijumon Kunjachan, Alexandre Detappe, Rajiv Kumar, Thomas Ireland, Lisa Cameron, Douglas E. Biancur, Vincent Motto-Ros, Lucie Sancey, Srinivas Sridhar, G. Mike Makrigiorgos, Ross I. Berbeco
Review
Chemistry, Analytical
Vincent Gardette, Vincent Motto-Ros, Cesar Alvarez-Llamas, Lucie Sancey, Ludovic Duponchel, Benoit Busser
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Inorganic & Nuclear
Amlia Godard, Laura Abad Galan, Jean Rouillon, Shaymaa Al Shehimy, Wassima Tajani, Charlotte Cave, Raluca Malacea-Kabbara, Yoann Rousselin, Pierre Le Gendre, Arnaud Fihey, Mohamed Bendellaa, Benoit Busser, Lucie Sancey, Boris Le Guennic, Christophe Bucher, Olivier Maury, Christine Goze, Ewen Bodio
Summary: Aza-boron-dipyrromethenes (Aza-BODIPYs) are a class of fluorophores with azadipyrromethene (aza-DIPY) ligand rigidified by a boron atom. Complexes of group 13 metals (aluminum and gallium) were synthesized and their properties were investigated. AzaGaDIPY and azaAlDIPY exhibited significant red-shifted fluorescence compared to aza-BODIPY, making them promising for optical medical imaging.
INORGANIC CHEMISTRY
(2023)
Article
Spectroscopy
Jorg Hermann, Christoph Gerhard, Milos Burger, Valentin Craciun, Frederic Pelascini
Summary: Direct elemental analysis of materials using calibration-free laser-induced breakdown spectroscopy has attracted significant interest since 1999. However, the multitude of different approaches and their validation in various experimental conditions make it challenging to assess the achievable performance of this innovative analytical technique. This paper critically reviews the development of calibration-free LIBS, highlights the progress made so far, and estimates its analytical performance. Solutions are proposed to enhance accuracy by addressing sources of measurement uncertainty, thereby bringing calibration-free LIBS to the level of fully recognized analytical methods.
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
(2023)
Editorial Material
Pharmacology & Pharmacy
Elisabeth Garanger, Nathalie Mignet, Chantal Pichon, Marie-Pierre Rols, Lucie Sancey
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Medicinal
Amelie Godard, Ghadir Kalot, Malorie Privat, Mohamed Bendellaa, Benoit Busser, K. David Wegner, Franck Denat, Xavier Le Guevel, Jean-Luc Coll, Catherine Paul, Ewen Bodio, Christine Goze, Lucie Sancey
Summary: Using fluorescence-guided surgery (FGS) with NIR-II dyes targeting human epidermal growth factor receptor 2 (HER2)-positive ovarian tumors showed favorable tumor accumulation and selective targeting of HER2 positive tumors. These bioconjugated NIR-II-emitting dyes displayed prolonged stability in serum and maintained affinity toward HER2 in vitro, indicating their potential for NIR-II FGS in the cancer setting. This study suggests that the use of NIR-II dyes in FGS can improve the complete resection of microscopic ovarian tumors.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Review
Oncology
Mohamed Bendellaa, Pierre Lelievre, Jean-Luc Coll, Lucie Sancey, Aurelien Deniaud, Benoit Busser
Summary: Zinc is a crucial element involved in various cellular processes and dysregulation of zinc homeostasis occurs in certain cancers. This review explores the role of zinc in cancer, including its related proteins as potential biomarkers and the use of zinc-based strategies for tumor treatment. Targeting zinc and its pathways offers therapeutic approaches, while direct utilization of zinc as a therapeutic agent holds promise for improving cancer prognosis.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Spectroscopy
Piyali Deb Barman, Ashok Kumar Maurya, Subhendu Ghosh, Ankit Roy, Mukul Madaan
Summary: In X-ray spectroscopic studies, K beta 1,3 lines have been used to examine the chemical states of 3d transition metals, but are not suitable for characterizing iron oxides. Instead, FeL-lines, which involve the valence shell 3d electrons, were inspected and found to provide information on the chemical states of iron. The study established a linear relationship between the chemical shift of FeL beta 1 line and spin multiplicity, allowing for the speciation of iron.
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
(2024)
