Journal
SOFT MATTER
Volume 13, Issue 10, Pages 1998-2003Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6sm02107g
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Funding
- Deutsche Forschungsgemeinschaft [HE2995/3-1, INST 215/424-1 FUGG]
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We study the diffusion of an enhanced green fluorescent protein (GFP+) in bicontinuous sugarsurfactant based microemulsions. The size of the water domains in such systems is controlled by changes of the oil-to-water ratio. Hence, microemulsions allow to produce confinement effects in a controlled way. At high water content the protein is found to exhibit Fickian diffusion. Decreasing the water domain size leads to a slowing down of the protein diffusion and sub-diffusive behavior is obtained on the scale observed by fluorescence correlation spectroscopy. Further decrease of the water domain size finally nearly fixes the GFP+ in these domains and forces it to increasingly follow the breathing mode of the microemulsion matrix.
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