Article
Biochemistry & Molecular Biology
Daniel Adon Mapamba, Elingarami Sauli, Julieth Lalashowi, Joram Buza, Joseph John, Zawadi Mwaisango, Peter Tarmo, Issa Sabi, Andrea Rachow, Nyanda Elias Ntinginya, Bariki Mtafya
Summary: This study evaluated the utility of Tuberculosis Molecular Bacterial Load Assay (TB-MBLA) and lipoarabinomannan (LAM) in detecting M. tb in HIV co-infections. The results showed that the positivity rates of TB-MBLA and urine LAM were 4.8% and 14.3%, respectively, while culture and microscopy of sputum counterparts had positivity rates of 20.6% and 12.7%, respectively. The study found that TB-MBLA has the potential to improve the detection of M. tb in urine of HIV co-infected patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medical Laboratory Technology
Jun Ni, Wenbo Zhu, Yanyang Wang, Xuefei Wei, Jingjing Li, Lu Peng, Kui Zhang, Bing Bai
Summary: This study introduced a reference chart to deal with the interference of hemolyzed blood samples in clinical biochemical tests, providing color appearance guidance for clinical staff to determine sample acceptability. The preparation of such a reference chart is extremely useful in clinical laboratories.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2021)
Article
Medicine, General & Internal
Harika Oykue Dinc, Nuran Karabulut, Sema Alacam, Hayriye Kirkoyun Uysal, Ferhat Osman Dasdemir, Mustafa Onel, Yesim Tuyji Tok, Serhat Sirekbasan, Ali Agacfidan, Nesrin Gareayaghi, Huseyin cakan, Onder Yuksel Eryigit, Bekir Kocazeybek
Summary: This study assessed the performance of a rapid antigen test for detecting SARS-CoV-2, IAV, and IBV compared to the rRT-PCR method. The sensitivity of the test was high for SARS-CoV-2 and IAV at viral loads below 20 Ct, but lower for viral loads above 20 Ct. The test showed 100% specificity. Rapid antigen tests may be useful for screening in communal settings, especially for symptomatic individuals, but caution is needed for lower viral loads.
Article
Medicine, General & Internal
Kristin Widyasari, Soomin Kim, Sunjoo Kim, Chae Seung Lim
Summary: We evaluated the performance of the STANDARD Q COVID/FLU Ag Combo test for the detection of SARS-CoV-2, influenza A, and influenza B using a single point-of-care device compared with real-time PCR. The test showed high sensitivity and specificity for detecting these viruses, with excellent agreement with the results of RT-PCR. This makes it a useful and efficient tool for detecting SARS-CoV-2, influenza A, and influenza B.
Article
Chemistry, Analytical
Paola Cecere, Francesca Gatto, Claudia Cortimiglia, Daniela Bassi, Franco Lucchini, Pier Sandro Cocconcelli, Pier Paolo Pompa
Summary: Clostridium tyrobutyricum is the main spoiling agent in hard and semi-hard cheeses, leading to late blowing defects. The development of a point of care testing method combining fast DNA extraction and robust amplification techniques allows for sensitive and specific detection of this bacterium in milk samples, with the potential for semi-quantitative discrimination of contamination levels. The feasibility of this strategy using a portable battery-operated device for on-site quality control applications has been demonstrated, showing promise for industrial use without the need for sophisticated instrumentation or trained personnel.
Article
Public, Environmental & Occupational Health
Heather Rilkoff, Hadia Hussain, Juan Liu, Ken English, Joanne Lush, Ashleigh Sullivan, Vanessa Tran, Vanessa Allen, Michelle Murti
Summary: The objective of this study was to describe the changes in Ontario's Point-of-Care (POC) testing program before, during and after the focus on high-risk populations for HIV screening. The results showed a decrease in overall testing volumes, an increase in percent positivity, and higher positive predictive value (PPV) for higher-risk populations compared to lower-risk populations.
Article
Medicine, General & Internal
Christiana Graf, Inken Wagener, Katharina Grikscheit, Sebastian Hoehl, Annemarie Berger, Nils Wetzstein, Julia Dietz, Georg Dultz, Florian Michael, Natalie Filmann, Eva Herrmann, Peter Tinnemann, Udo Goetsch, Sandra Ciesek
Summary: This study found that olfactory dysfunction is common in patients with both positive and negative COVID-19 tests, but its diagnostic accuracy is not high enough to be used as an early identification tool for COVID-19 infection.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medical Laboratory Technology
Nicole Mueller, Laurenz Schneider, Johannes Breuer, Noa Judith Freudenthal
Summary: Analyzing NT-proBNP in urine using the cobas h232 POC system is feasible, but the detection range needs to be adjusted. Additionally, urine NT-proBNP remains stable for at least 96 hours at room temperature, making it suitable for postal shipping.
CLINICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Dimitra Tsounidi, Vasileios Tsaousis, Nikolaos Xenos, Christos Kroupis, Paraskevi Moutsatsou, Vasileios Christianidis, Dimitrios Goustouridis, Ioannis Raptis, Sotirios Kakabakos, Panagiota Petrou
Summary: This study presents the determination of two inflammatory diseases biomarkers, procalcitonin (PCT) and interleukin-6 (IL-6), in human serum samples using a portable device based on Multi Area Reflectance Spectroscopy. The device utilizes silicon chips with different thicknesses of silicon dioxide areas, functionalized with specific antibodies for PCT and IL-6, to achieve dual-analyte detection. The assay employs immobilized capture antibodies and biotinylated detection antibodies, along with streptavidin and biotinylated-BSA, to react with PCT and IL-6 calibrators. The assay is automated, providing quick and accurate results, and has the potential for point-of-need determination of inflammatory biomarkers.
Article
Microbiology
Haley L. DeMers, Teerapat Nualnoi, Peter Thorkildson, Derrick Hau, Emily E. Hannah, Heather R. Green, Sujata G. Pandit, Marcellene A. Gates-Hollingsworth, Latsaniphone Boutthasavong, Manophab Luangraj, Kate L. Woods, David Dance, David P. AuCoin
Summary: This study compared the levels of CPS in paired serum and urine samples from melioidosis patients and found higher concentrations in urine. The AMD LFI detected CPS in a higher percentage of urine samples compared to serum samples, suggesting that urine is a preferable matrix for point-of-care diagnostic assays for melioidosis.
MICROBIOLOGY SPECTRUM
(2022)
Editorial Material
Microbiology
Rayeil J. Chua, Rupert Capina, Hezhao Ji
Summary: Effective management of HIV drug resistance is crucial for achieving the goal of ending the AIDS epidemic. Point-of-care tests provide a convenient and fast solution for decentralized resistance monitoring, especially in low-to-middle-income countries where HIV treatment access is expanding globally and enhanced monitoring is urgently needed.
Article
Chemistry, Multidisciplinary
Juanzu Liu, Hongmin Meng, Lin Zhang, Shasha Li, Juan Chen, Yi Zhang, Jianjun Li, Lingbo Qu, Zhaohui Li
Summary: A simple dual-readout lateral flow test strip platform was developed for sensitive detection of alkaline phosphatase (ALP) based on a portable device. The method utilizes colorimetric and fluorescent signals to quantitatively detect trace amounts of ALP, showing great potential for clinical diagnosis.
CHINESE CHEMICAL LETTERS
(2021)
Article
Medicine, General & Internal
Penchom Janwan, Pewpan M. Intapan, Lakkhana Sadaow, Rutchanee Rodpai, Hiroshi Yamasaki, Patcharaporn Boonroumkaew, Oranuch Sanpool, Tongjit Thanchomnang, Phuangphaka Sadee, Wanchai Maleewong
Summary: The GB-ICT kit, based on recombinant Gnathostoma spinigerum antigen, can detect specific IgG4 antibody in whole-blood samples. It has high diagnostic accuracy, is convenient to use in remote areas and field settings without sophisticated equipment.
Article
Virology
Elaine Monteiro Matsuda, Cintia Mayumi Ahagon, Luana Portes Ozorio Coelho, Ivana Barros Campos, Daniela Rodrigues Colpas, Norberto Camilo Campos, Giselle Ibete Silva Lopez-Lopes, Valeria Oliveira Silva, Isabela Penteriche de Oliveira, Luis Fernando de Macedo Brigido
Summary: In an over 10-year effort from 2011 to 2021, the outcome of diagnosing acute and early HIV infections (AEHI) using a simple algorithm was documented in an Infectious Diseases Outpatient Clinic with limited resources. Of a total of 132 HIV-RNA tests conducted from 2017 to 2020, 12 cases were identified, showing successful diagnosis rates ranging from 2.4% among asymptomatic cases to 25% for undetermined serology cases, indicating the applicability of this screening method in different settings.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Chemistry, Analytical
Huimin Wan, Yufei Wang, Juan Chen, Hong-Min Meng, Zhaohui Li
Summary: A two-dimensional Co-MOF nanosheet-based nanozyme was developed for colorimetric detection of ascorbic acid and alkaline phosphatase. The nanozyme exhibited high catalytic activity and was successfully used for clinical sample detection.