Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 251, Issue -, Pages 302-309Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.05.039
Keywords
Prostate cancer antigen 3 (PCA3); Surface-enhanced Raman scattering; Amplification-free DNA detection; Hollow gold nanospheres; Polymerase chain reaction (PCR)
Funding
- National Research Foundation of Korea [2008-0061891, 2009-00426]
- Nano Material Technology Development Program through the National Research Foundation of Korea - Ministry of Science, ICT & Future Planning [2012M3A7B4035288]
- Korea Health Industry Development Institute (KHIDI) - Ministry of Health and Welfare, Korea [HI16C2129]
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We report the development of amplification-free surface-enhanced Raman scattering (SERS)-based DNA assays for the rapid and highly sensitive detection of prostate cancer antigen 3 (PCA3) mimic DNA. This technique does not require any DNA amplification process using thermo-cycles in conventional polymerase chain reaction (PCR) due to its highly sensitive detection capability. Herein, the PCA3 mimic DNA, composed of 45 nucleotide sequences, was sandwiched between two probe DNA-immobilized particles (ASO(735)-conjugated detection HGNs and ASO(683)-immobilized capture magnetic beads) by hybridization reactions. Its quantitative analysis was performed by monitoring the characteristic Raman peak intensity of sandwich DNA complexes. The limit of detection (LOD) is estimated to be 2.7 fM, which is about four orders of magnitude more sensitive than that of conventional PCR. This SERS-based DNA assay technique is expected to be a potentially useful tool for early disease diagnosis. (C) 2017 Elsevier B.V. All rights reserved.
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