Article
Biochemistry & Molecular Biology
Zheng Wei, Xiaoping Cai, Weifeng Cui, Junping Zhang
Summary: A CA19-9 electrochemical immunosensor was developed using a self-assembled membrane modified with a gold electrode. It demonstrated good specificity, strong anti-interference ability, and high precision and accuracy. The results showed its potential application value.
Article
Chemistry, Analytical
Hessamaddin Sohrabi, Mir Reza Majidi, Karim Asadpour-Zeynali, Alireza Khataee, Ahad Mokhtarzadeh
Summary: This research demonstrates a label-free electrochemical-based genosensing assay for the sensitive detection of the L-fuculokinase genome related to Haemophilus influenzae (H. influenzae). Multiple electrochemical modifier-tagged agents were effectively used to enhance the electrochemical responses. The designed genosensing bio-platform achieved low detection and quantification limits (LOD and LOQ) of 6.14 fM and 11 fM, respectively, and demonstrated a wide linear range of 0.1 to 1000 pM. The assay showed high selectivity, good performance, and reliable repeatability and reproducibility, making it a promising method for rapidly and quantitatively detecting H. influenzae.
Article
Chemistry, Applied
Karutha Pandian Divya, Venkataraman Dharuman
Summary: A novel and rapid electrochemical immunosensing platform was developed for directly sensing antibody interactions with a virulence factor, utilizing various techniques to study molecular interactions and sensor performance.
Article
Chemistry, Analytical
Jairo Pinto de Oliveira, Francisco Burgos-Florez, Isabella Sampaio, Pedro Villalba, Valtencir Zucolotto
Summary: In this study, a label-free impedimetric immunosensor was developed to detect OTA in coffee. The biosensor showed high selectivity and was not affected by matrix interference in coffee samples. It has the advantages of small sample volume, short analysis time, and potential miniaturization.
Article
Chemistry, Analytical
Viviana Vasquez, Jahir Orozco
Summary: Cases of SARS-CoV-2 are still widespread due to the emergence of new variants worldwide. The severity and infectiousness of the virus are still not fully understood. Therefore, there is an urgent need for improved viral detection and diagnosis tools.
MICROCHEMICAL JOURNAL
(2023)
Article
Biophysics
Baha Ondes, Sinem Evli, Murat Uygun, Deniz Aktas Uygun
Summary: A new label-free electrochemical immunosensor modified with boron nitride nanosheets was developed for early detection of CA125 in this study. The immunosensor showed good detection properties and stability, successfully detecting CA125 in artificial serum samples. The prepared immunosensor has potential for point-of-care diagnosis in real cases.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemical Research Methods
Rosaceleste Zumpano, Manuela Manghisi, Francesca Polli, Cristine D'Agostino, Federica Ietto, Gabriele Favero, Franco Mazzei
Summary: This study developed a label-free electrochemical immunosensor for the quick, cheap, and straightforward determination of atrazine, achieving detection through a technological platform and specific treatment methods. Under optimal experimental conditions, the immunosensor exhibited good linearity range, limit of detection, reproducibility, and stability, making it suitable for successful application in the detection and monitoring of atrazine in real water samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Dexin Du, Jue Wang, Mingquan Guo, Jiangnan Shu, Wei Nie, Zhiping Bian, Di Yang, Hua Cui
Summary: Label-free electrochemiluminescence (ECL) immunoassays (lf-ECLIA) have become an attractive method for biomarker detection due to their simplicity, speed, and cost-effectiveness. This study investigated the interaction between biomarkers and analytical interfaces constructed using a nanoluminophore and antibody-functionalized gold nanoparticles. The results showed that positively charged biomarkers increased the ECL intensity, while negatively charged biomarkers decreased the ECL intensity. A charge-dependent lf-ECLIA method was established based on the ECL signal changes, which exhibited different responses to positively charged and negatively charged biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xinke Liu, Ching-Li Tseng, Lu-Yin Lin, Chin-An Lee, Jian Li, Li Feng, Lijun Song, Xiaohua Li, Jr-Hau He, Rajalakshmi Sakthivel, Ren-Jei Chung
Summary: The study developed a LEP immunosensor using Ce3NbO7/CeO2 as a new material, demonstrating high sensitivity, a wide linear range, and a low detection limit. By improving electrode conductivity and surface functionalization, successful LEP immunosensing was achieved, applicable for plasma samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Danilo Echeverri, Jahir Orozco
Summary: This study reports an electrochemical immunosensor for the detection of the novel beta-1,4-GalT-V CRC biomarker. The immunosensor covalently couples an anti-beta-1,4-GalT-V antibody on a self-assembled monolayer-coated gold electrode surface and shows high specificity in capturing the beta-1,4-GalT-V glycoprotein from human serum samples. The response of the immunosensor, monitored by electrochemical impedance spectroscopy, is protein concentration-dependent.
Article
Multidisciplinary Sciences
Jantima Upan, Napaporn Youngvises, Adisorn Tuantranont, Chanpen Karuwan, Philippe Banet, Pierre-Henri Aubert, Jaroon Jakmunee
Summary: A label-free electrochemical aptamer-based sensor was developed for AFP detection, utilizing a PtNPs/GO-COOH modified platform with an AFP aptamer as the recognition element. The sensor demonstrated good sensitivity, selectivity, and stability for sensitive detection of AFP in serum samples.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Pushap Raj, Man Hwan Oh, Kyudong Han, Tae Yoon Lee
Summary: Developed a simple, label-free, and highly sensitive immunosensor for early diagnosis of bacterial pathogens using Au@Mo-2-PANI nanocomposite. Employed a self-assembled monolayer of mercaptopropionic acid on the gold nanoparticle surface for covalent immobilization of antibodies to minimize nonspecific adsorption of bacterial pathogens. The biosensor showed high selectivity and sensitivity with a low limit of detection, detecting E. coli within 30 min and demonstrating practical utility in urine samples.
Article
Chemistry, Analytical
Zhihao Feng, Xiaoyi Li, Changxiang Fang, Junying Li, Rongfei Wang, Weihua Hu
Summary: Oblique-incidence reflectivity difference (OIRD) is a newly established method with great potential for real-time and label-free immunoassay. This study developed a label-free OIRD immunoassay biochip with improved sensitivity by using a standard glass slide coated with polystyrene (PS) microsphere monolayer as the substrate. The immunoassay sensitivity of this biochip was greatly improved compared to the conventional biochip on glass slide, and the improvement was attributed to the high capture efficiency and enhanced intrinsic OIRD sensitivity of the porous PS monolayer. This work provides valuable insights for the development of high-performance label-free OIRD bio-detection devices.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemical Research Methods
Najib Ben Messaoud, Marilia Barreiros dos Santos, Ana Vieira, Alejandro Garrido-Maestu, Begona Espina, Raquel B. Queiros
Summary: Infectious diseases caused by A. salmonicida have significant impacts on aquaculture and fish farming. This study presents a portable label-free electrochemical immunosensor for the detection of A. salmonicida in seawater, which is sensitive and specific.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Huali Jin, Jie Dong, Xinru Qi, Xiaoxia Sun, Min Wei, Baoshan He, Zhiguang Suo
Summary: This study developed a label-free impedance-based electrochemical sensor for the quantitative detection of Pb2+. The use of conductive gold nanomaterials as electrode substrate materials provides sensors with a larger specific surface area, action sites, and excellent conductivity. DNA nanostructures were utilized for biomolecule determination, leading to successful quantitative detection of Pb2+. The designed sensors demonstrated good recoveries in real samples, making it highly applicable in various fields.
BIOELECTROCHEMISTRY
(2023)
Article
Biochemical Research Methods
Hedieh Haji-Hashemi, Mohammad Reza Safarnejad, Parviz Norouzi, Mehrnaz Ebrahimi, Morteza Shahmirzaie, Mohammad Reza Ganjali
ANALYTICAL BIOCHEMISTRY
(2019)
Article
Chemistry, Analytical
Mohammad Reza Moghaddam, Jahan B. Ghasemi, Parviz Norouzi, Foad Salehnia
MICROCHEMICAL JOURNAL
(2019)
Article
Engineering, Electrical & Electronic
Parviz Norouzi, Ahmad Karimpour, Mohammad Reza Ganjali
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
(2019)
Article
Plant Sciences
Hamideh Raeisi, Mohammad Reza Safarnejad, Seyed Mehdi Alavi, Seyed Ali Elahinia, Naser Farrokhi
JOURNAL OF PLANT PATHOLOGY
(2020)
Article
Chemistry, Analytical
Maedeh Akhoundian, Taher Alizadeh, Mohammad Reza Ganjali, Parviz Norouzi
Article
Biochemical Research Methods
Morteza Shahmirzaie, Mohammad Reza Safarnejad, Farshad Rakhshandehroo, Hossein Safarpour, Farshad H. Shirazi, Hamid Reza Zamanizadeh, Toufic Elbeaino
JOURNAL OF VIROLOGICAL METHODS
(2020)
Article
Engineering, Multidisciplinary
Hadi Beitollahi, Mohammad Reza Ganjali, Parviz Norouzi, Kaveh Movlaee, Rahman Hosseinzadeh, Somayeh Tajik
Article
Chemistry, Analytical
Ilnaz Soleimani Mashhadi, Mohammad Reza Safarnejad, Morteza Shahmirzaie, Atousa Aliahmadi, Alireza Ghassempour
ANALYTICAL CHEMISTRY
(2020)
Article
Virology
Hamideh Raeisi, Mohammad Reza Safarnejad, Pedram Moeini, Hossein Safarpour, Yalda Sokhansanj
ARCHIVES OF VIROLOGY
(2020)
Article
Chemistry, Analytical
Parviz Norouzi, Mohammad Nezamoddini, Mohammad Reza Safarnejad
Summary: This study presents a new electrochemical immunosensor based on a modified GC electrode and fast Fourier transforms square wave voltammetry for sensitive and rapid detection of IBDV. The immunosensor showed excellent selectivity, stability, and sensitivity.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2023)
Article
Horticulture
S. Karimpour, G. H. Davarynejad, M. ZakiAghl, M. R. Safarnejad, P. Martinez-Gomez, M. Rubio
Summary: Production of virus-free plants is crucial for vegetative propagation. In vitro thermotherapy treatments can effectively eliminate viruses in apple shoots, leading to significantly faster growth in virus-free plants. Rapid assessment of physiological, growth and sanitary state of apple shoots can be performed through chlorophyll content evaluation using SPAD-502 meter.
EUROPEAN JOURNAL OF HORTICULTURAL SCIENCE
(2021)
Article
Mycology
Saeede Ansari, Amir Mousavi, Mohammad Reza Safarnejad, Naser Farrokhi, Seyed Mehdi Alavi, Stefan Schillberg, Greta Noelke
Summary: Aspergillus flavus is a major fungal pathogen of plants and an opportunistic pathogen of humans. Early detection of A. flavus is crucial for diagnosing and monitoring fungal infection, preventing contamination, and effective therapy. Aspergillus-specific monoclonal antibodies show promise for diagnostic and therapeutic purposes by specifically binding to A. flavus antigens.
Article
Chemistry, Multidisciplinary
Parviz Norouzi, Mohammad Nezamoddini, Mohammad Reza Safarnejad
Summary: A new label-free electrochemical immunosensor was developed for detecting Newcastle disease virus (NDV) with high sensitivity and stability, making it suitable for agricultural applications.
Proceedings Paper
Agronomy
S. Karimpour, G. H. Davarynejad, M. Zaki-AGhl, M. R. Safarnejad, N. Alburquerque, M. D. Nortes, L. Burgos, M. Rubio, P. Martinez-Gomez
XVII INTERNATIONAL SYMPOSIUM ON APRICOT BREEDING AND CULTURE
(2020)
Article
Agriculture, Multidisciplinary
S. Karimpour, G. Davarynejad, M. Zaki Aghl, M. R. Safarnejad
JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY
(2020)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
