Journal
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Volume 22, Issue 14, Pages 10758-10768Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-015-4263-7
Keywords
Comet assay; Planaxis sulcatus; Mercuric chloride; Genotoxicity; DNA damage; In vivo; In vitro
Categories
Funding
- Department of Biotechnology, New Delhi
- Council of Scientific and Industrial Research (CSIR)
- [PSC0206]
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In vivo and in vitro exposures were used to investigate the genotoxicity of mercuric chloride (HgCl2) to the marine snail, Planaxis sulcatus. The comet assay protocol was validated on gill cells exposed in vitro to hydrogen peroxide (H2O2, 0-50 mu M). Snails were exposed in vivo for 96 h to HgCl2 (10, 20, 50, and 100 mu g/l). Our results showed significant concentration-dependent increase in the tail DNA (TDNA) and olive tail moment (OTM) in exposed snails for all doses compared with controls. In vitro exposure to HgCl2 (10-100 mu g/l) resulted in significantly higher values for TDNA at all concentrations. Our results showed that DNA damage increased in the gill cell with increasing exposure time. This study demonstrates the usefulness of comet assay for detection of DNA damage after exposure to HgCl2 and the sensitivity of marine snail P. sulcatus as a good candidate species for metal pollution.
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