Article
Cell Biology
Elizabeth J. Haining, Kate L. Lowe, Surasak Wichaiyo, Raghu P. Kataru, Zoltan Nagy, Dean Pj Kavanagh, Sian Lax, Ying Di, Bernhard Nieswandt, Benoit Ho-Tin-Noe, Babak J. Mehrara, Yotis A. Senis, Julie Rayes, Steve P. Watson
Summary: The study found that CLEC-2 expression on platelets is not necessary to maintain a barrier between the blood and lymphatic systems in unchallenged mice, but deficiency in CLEC-2 can lead to lymphatic vessel blood filling under conditions of chronic vascular remodeling. CLEC-2-driven platelet activation plays an essential role in vascular developmental programs.
Review
Immunology
Danyang Meng, Man Luo, Beibei Liu
Summary: CLEC-2, expressed on platelets and immune cells, plays a crucial role in infection and immunity, participating in processes such as thromboinflammation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Hematology
Joshua H. Bourne, Christopher W. Smith, Natalie J. Jooss, Ying Di, Helena C. Brown, Samantha J. Montague, Mark R. Thomas, Natalie S. Poulter, Julie Rayes, Steve P. Watson
Summary: C-type lectin-like receptor 2 (CLEC-2) is highly expressed on platelets and plays a critical role in lymphatic development. This study demonstrates that CLEC-2 supports thrombus formation at sites of inflammation without affecting hemostasis. It suggests that CLEC-2 could be a promising therapeutic target for thromboinflammatory disorders.
THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
Toshiaki Shirai, Nagaharu Tsukiji, Tomoyuki Sasaki, Saori Oishi, Ryohei Yokomori, Katsuhiro Takano, Katsue Suzuki-Inoue
Summary: This study reveals that cancer-associated fibroblasts (CAFs) and their released extracellular vesicles (EVs) can induce CLEC-2-dependent platelet aggregation, exacerbating venous thrombosis.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Immunology
Joshua H. Bourne, Nonantzin Beristain-Covarrubias, Malou Zuidscherwoude, Joana Campos, Ying Di, Evelyn Garlick, Martina Colicchia, Lauren Terry, Steven G. Thomas, Alexander Brill, Jagadeesh Bayry, Steve P. Watson, Julie Rayes
Summary: Platelet CLEC-2 reduces tissue inflammation by regulating inflammatory macrophage activation and trafficking, depending on the expression of its ligand podoplanin. This immune regulatory function is independent of platelet activation and secretion, and involves actin rearrangement, macrophage migration, and modulation of pro-inflammatory cytokines and immunosuppressive factors.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Cell Biology
Eleyna M. Martin, Malou Zuidscherwoude, Luis a Moran, Ying Di, Angel Garcia, Steve P. Watson
Summary: The platelet C-type lectin-like receptor CLEC-2 plays a role in inflammation-driven venous thrombosis, making it a potential target for new antiplatelet agents. Understanding the structure and dynamics of CLEC-2 on the platelet membrane, as well as its interactions with Syk, is crucial for the development of therapeutics.
Article
Oncology
Mani Etemad, Foteini Christodoulou, Stefanie Uhlig, Jessica C. Hassel, Petra Schrotz-King, Hermann Brenner, Cornelia M. Ulrich, Karen Bieback, Harald Klueter, Peter Bugert
Summary: This study investigated the effect of platelet activation on CLEC-2 expression and sCLEC-2 plasma level. PDPN significantly increased the aggregation response induced by classical agonists. Stimulation of platelets with ADP or PDPN caused a significant decrease in CLEC-2 on platelets and sCLEC-2 in the plasma. The plasma level of sCLEC-2 was lower in most tumor patients, except for glioblastoma patients who displayed higher levels.
Article
Immunology
Alexander A. Fields, Elizabeth Andraska, Christof A. Kaltenmeier, Zachary Matthay, Kimberly Herrera, Brenda M. Nunez-Garcia, Chayse D. Jones, Katherine Wick, Silvia Liu, Jian-Hua Luo, Yan-Ping A. Yu, Michael M. Matthay, Carolyn J. Hendrickson, Roland J. Bainton, Tessa S. Barrett, Jeffrey D. Berger, Matthew Z. Neal, Lucy Kornblith
Summary: This study found that plasma from COVID-19 patients can induce abnormal platelet function and promote inflammatory response in neutrophils. The soluble components in plasma had an impact on the behavior of platelets and neutrophils, which may contribute to the thromboinflammatory complications observed in COVID-19.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Chaojun Tang, Lei Wang, Yulan Sheng, Zhong Zheng, Zhanli Xie, Fan Wu, Tao You, Lijie Ren, Lijun Xia, Changgeng Ruan, Li Zhu
Summary: This study demonstrated that disturbed blood flow can induce platelet accumulation in atherosclerosis, with CLEC-2 gene knockout potentially reducing subendothelial accumulation of platelets and monocytes/macrophages, thereby ameliorating plaque formation.
Review
Cell Biology
Cecile Oury, Odile Wera
Summary: The article discusses the role of the ATP-gated P2X1 ion channel in thromboinflammation, focusing on its interaction between platelets and neutrophils, particularly in various disease states or conditions.
Review
Hematology
Nagaharu Tsukiji, Katsue Suzuki-Inoue
Summary: Lymphatic vessels play a crucial role in maintaining interstitial fluid homeostasis and regulating immune responses. Platelets are essential for separating lymph sacs from veins and regulating lymphangiogenesis. The hemostatic system is involved in the establishment and maintenance of lymphatic networks.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Review
Pharmacology & Pharmacy
Yi Wang, Dan Peng, Yaqian Huang, Yongjun Cao, Hui Li, Xia Zhang
Summary: Podoplanin is a protein involved in various physiological and pathological processes in the brain. It is upregulated in different cell types and interacts with other molecules. Past studies have summarized its pleiotropic roles in the periphery, while recent research focuses on its distribution and function in the brain. This review summarizes the roles and mechanisms of Podoplanin in the development and disorders of the nervous system and discusses the challenges of using Podoplanin as a target for disease prognosis and prevention.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biology
Joanne C. Clark, Eleyna M. Martin, Luis A. Moran, Ying Di, Xueqing Wang, Malou Zuidscherwoude, Helena C. Brown, Deirdre M. Kavanagh, Johan Hummert, Johannes A. Eble, Bernhard Nieswandt, David Stegner, Alice Y. Pollitt, Dirk-Peter Herten, Michael G. Tomlinson, Angel Garcia, Steve P. Watson
Summary: Fluorescence correlation spectroscopy was used to investigate the clustering of CLEC-2 induced by crosslinked nanobody ligands. The results showed that the valency of the ligands and the expression levels of CLEC-2 were important factors in determining the activation of CLEC-2. Divalent ligands were found to be partial agonists while tetravalent ligands stimulated platelet aggregation.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biology
Daria S. Morozova, Alexey A. Martyanov, Sergei Obydennyi, Julia-Jessica D. Korobkin, Alexey Sokolov, Ekaterina Shamova, Irina Gorudko, Anna L. Khoreva, Anna Shcherbina, Mikhail A. Panteleev, Anastasia N. Sveshnikova
Summary: In this study, an ex vivo experimental model for observation of granulocyte activity in thrombus formation was developed. Results showed that granulocytes from patients with WAS had reduced motility compared to healthy donors. The study suggests that WASP deficiency disrupts granulocyte behavior in thromboinflammatory conditions.
Review
Hematology
Shruti Raghunathan, Julie Rayes, Anirban Sen Gupta
Summary: Platelets, derived from megakaryocytes, play a vital role in hemostasis and can be used as a platform for disease-targeted drug delivery. Platelet defects can lead to bleeding complications, while platelet-inspired technologies have the potential to improve therapeutic efficacy.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)