Article
Chemistry, Physical
Jiani Lei, Shanshan Li, Shuang Liu, Qingyuan Wu, Bolong Xu, Zhijun Huang, Nier Wu, Xiaolu Xiong, Huiyu Liu, Dongsheng Zhou
Summary: This study presents a composite hemostatic chitosan sponge CaO2@SiO2/CS that can achieve rapid blood clotting and antimicrobial effects. The material reduces cytotoxicity and the risk of secondary bleeding while promoting quick hemostasis.
Article
Materials Science, Multidisciplinary
Yeping Song, Yi Zhu, Qinghua Wang, Xin Chen, Yu Chen, Guohao Han, Weipeng Lu, Yanchuan Guo
Summary: The advantages of an ideal hemostasis material for emergency rescue are significant in mitigating patient distress and minimizing threat to life. However, obtaining a single material with desirable properties such as safety, antibacterial activity, high efficiency, sufficient mechanical strength, and cost-effectiveness remains challenging.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Nikou Fotouhi, Michaela Fischer-Stettler, Gioia Lenzoni, Stephanie Stolz, Gaetan Glauser, Samuel C. Zeeman, Edward E. Farmer
Summary: This study demonstrates the important role of ACA10 and ACA12 genes in maintaining tissue excitability and defense against insect attack in Arabidopsis. Additionally, the findings suggest that the plant vasculature plays a crucial role in electrical signaling failure.
Article
Critical Care Medicine
Nina Buchtele, Michael Schwameis, Peter Schellongowski, Peter Quehenberger, Paul Knoebl, Ludwig Traby, Monika Schmid, Christian Schoergenhofer, Harald Herkner, Bernd Jilma, Thomas Staudinger
Summary: A high prevalence of reduced coagulation factor XII activity was observed in adult patients on extracorporeal membrane oxygenation, with lower activity associated with fewer thromboembolic complications. Coagulation factor XII activity may confound activated partial thromboplastin time measurements and potentially serve as a target for anticoagulation in ECMO.
CRITICAL CARE MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Yanxing Yang, Sharareh Jalali, Bradley L. Nilsson, Cristiano L. Dias
Summary: The study found that electrostatic and hydrophobic interactions play important roles in the peptide-bilayer binding process. Peptides are strongly attracted to anionic lipid bilayers due to electrostatic interactions between positive residues and negative phosphate moieties. However, the presence of calcium ions significantly reduces the binding of peptides to the bilayer.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Pediatrics
Madhuradhar Chegondi, Niranjan Vijayakumar, Aditya Badheka, Oliver Karam
Summary: Platelet transfusions do not appear to have an impact on the function of the ECMO oxygenator overall. However, in patients with lower pre-transfusion oxygenator function, platelet transfusions are associated with a worsening function.
FRONTIERS IN PEDIATRICS
(2022)
Article
Engineering, Biomedical
Teryn R. R. Roberts, George T. T. Harea, Yanyi Zang, Ryan P. P. Devine, Patrick Maffe, Hitesh Handa, Andriy I. I. Batchinsky
Summary: This study evaluated the use of a slippery liquid-infused nitric oxide-releasing material modification for blood circulation tubing in extracorporeal lung support. The results showed that this modification had good blood compatibility and potential to reduce bleeding and clotting during extracorporeal organ support.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2023)
Article
Biology
Ruiqi Cai, Lingyun Wang, Xiong Liu, Marek Michalak, Jingfeng Tang, Ji-Bin Peng, Xing-Zhen Chen
Summary: TRPV6, a calcium-selective channel, plays a crucial role in calcium homeostasis and its specific mutation may promote the progression of breast cancer cells.
COMMUNICATIONS BIOLOGY
(2021)
Review
Hematology
Y. Zenmei Ohkubo, Jesper J. Madsen
Summary: In the field of life sciences, methods of structural biology and computational biology have become crucial tools for studying the underlying mechanisms of complex biological processes. Computational studies, combined with biochemical and biophysical approaches, have proven to be valuable assets in building structural models for membrane-bound forms of coagulation factors, aiding in understanding the molecular basis of hemostasis.
THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Biochemical Research Methods
Alexander J. Bryer, Tyler Reddy, Edward Lyman, Juan R. Perilla
Summary: Enveloped viruses are protected by a lipid membrane, and the complex lipid composition of the envelope is closely related to its structural, mechanical, and dynamical properties.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Sizhe Gao, Yang Zhang, Rui Peng, Shujie Yan, Yuan Teng, Jiachen Qi, Gaowa Cheng, Dongze Yu, Gang Liu, Qiang Xu, Xiaohui Cai, Zhou Zhou, Bingyang Ji
Summary: This study explored the effects of the ECMO system on the coagulation system in a healthy ovine ECMO model. It found that platelets decreased in the first 72 hours and neutrophils increased in the first 24 hours of ECMO. Various coagulation factors showed different patterns of change during ECMO. The study also observed thrombosis in some sheep but no clinically relevant bleeding or thrombosis events occurred.
Article
Engineering, Environmental
Ying Long, Genying Yu, Lu Dong, Yanchao Xu, Hongjun Lin, Ying Deng, Xiujia You, Lining Yang, Biao-Qiang Liao
Summary: This study investigated the effects of calcium ions and polyaluminum chloride on membrane fouling in the coagulation-ultrafiltration process, revealing synergistic effects between the two agents. By using density functional theory calculations and explaining the thermodynamic mechanisms, a theoretical explanation for such filtration behaviors was proposed.
Article
Hematology
Yang Zhang, Rui Peng, Shengqiang Pei, Sizhe Gao, Yang Sun, Gaowa Cheng, Dongze Yu, Ximing Wang, Zhangwei Gao, Bingyang Ji, Zhou Zhou
Summary: This study aimed to explore the role of NETs in thrombosis during ECMO support and investigate NETs as a predictive biomarker for thrombotic complications during ECMO assistance. The results showed that NETs might be associated with thrombus during ECMO support, especially in the VA mode. These findings provide new insight into preventing thrombotic complications by targeting NETs, and NETs may potentially become an early warning biomarker for thrombosis under ECMO assistance.
THROMBOSIS RESEARCH
(2023)
Article
Cell Biology
Andrew King On Wong, Barry Paul Young, Christopher J. R. Loewen
Summary: Our study demonstrates that Ist2 plays a crucial role in recruiting PS transport proteins and ORP family members Osh6 and Osh7 to ER-PM contacts, facilitating nonvesicular PS transport for PE synthesis in the endosomes/Golgi/vacuoles. This molecular link between the ORP and TMEM16 families highlights the specific role of Ist2 and Osh6/7 in the endocytosis of PS for PE production.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Cardiac & Cardiovascular Systems
Yuheng Lang, Yue Zheng, Bingcai Qi, Weifeng Zheng, Jin Wei, Chengxiu Zhao, Wenqing Gao, Tong Li
Summary: Nafamostat mesylate (NM) has important applications in extracorporeal life support, as it can reduce inflammation and promote patient recovery. This review focuses on the pharmacology, monitoring parameters, and special considerations for the use of NM in patients receiving extracorporeal life support.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2022)
Letter
Rheumatology
Melek Yalcin Mutlu, Jochen Wacker, Koray Tascilar, Jule Taubmann, Bernhard Manger, Gerhard Kronke, Georg Schett, David Simon
Article
Rheumatology
Koray Tascilar, Filippo Fagni, Arnd Kleyer, Sara Bayat, Robert Heidemann, Florian Steiger, Gerhard Kronke, Daniela Bohr, Andreas Ramming, Fabian Hartmann, Daniel Klett, Anna Federle, Adrian P. Regensburger, Alexandra L. Wagner, Ferdinand Knieling, Markus F. Neurath, Georg Schett, Maximilian Waldner, David Simon
Summary: MSOT can non-invasively measure metabolic differences between arthritis and enthesitis in vivo. Enthesitis-related sonographic changes are associated with increased total Hb, oxygen saturation, and collagen content, while arthritis-related clinical and sonographic findings are associated with increased Hb levels, reduced oxygen saturation, and reduced collagen content.
Article
Chemistry, Medicinal
Martina Salerno, Carmine Varricchio, Federica Bevilacqua, Dirk Jochmans, Johan Neyts, Andrea Brancale, Salvatore Ferla, Marcella Bassetto
Summary: Different viruses, such as enterovirus 71, rely on the host enzyme METTL3 to complete their cytoplasmic life cycle stages. By modulating the activity of this enzyme, it is possible to interfere with a broad range of viral infections. In this study, a series of nucleoside analogues were designed as inhibitors of human METTL3 to target multiple viral infections. Through molecular docking studies and synthesis, several novel and potent inhibitors of enterovirus 71 were identified.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Virology
Gilda Giancotti, Giulio Nannetti, Gilda Padalino, Martina Landini, Nanci Santos-Ferreira, Jana Van Dycke, Valentina Naccarato, Usheer Patel, Romano Silvestri, Johan Neyts, Roberto Gozalbo-Rovira, Jesus Rodriguez-Diaz, Joana Rocha-Pereira, Andrea Brancale, Salvatore Ferla, Marcella Bassetto
Summary: Human norovirus is the leading cause of foodborne diseases worldwide, resulting in severe acute gastroenteritis outbreaks and causing approximately 200,000 deaths in children in developing countries annually. Current treatment options are limited to supportive care, highlighting the urgent need for antiviral agents. In this study, we focused on the viral RNA-dependent RNA polymerase (RdRp) as a potential target for antiviral drug discovery. By rationally modifying identified scaffolds, we synthesized new compounds with improved inhibition of RdRp, providing a promising foundation for further optimization.
Letter
Hematology
Justin S. Trory, Attila Munkacsi, Kamila M. Sledz, Jordan Vautrinot, Lucy J. Goudswaard, Molly L. Jackson, Kate J. Heesom, Samantha F. Moore, Alastair W. Poole, Behnam Nabet, Varinder K. Aggarwal, Ingeborg Hers
Article
Hematology
Tessa Noordermeer, Rolf T. Urbanus, Chong Y. Wong, Janna J. Jansma, Nynke M. Wiersma, Minka Zivkovic, Albert Huisman, Maarten Limper
Summary: This study found significant discrepancies between INR values measured with the Coa-guChek XS and laboratory-INR in patients with antiphospholipid syndrome. High levels of anti-beta 2-glycoprotein I IgG antibodies were correlated with the differences between POCT-INR and laboratory-INR. Thus, laboratory-INR monitoring should be preferred over POCT-INR monitoring, especially in patients with high anti-beta 2-glycoprotein IgG antibody levels.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Pharmacology & Pharmacy
Jessica R. Spengler, Stephen R. Welch, Jerome Deval, Brian G. Gentry, Andrea Brancale, Kara Carter, Jennifer Moffat, Chris Meier, Katherine L. Seley-Radtke, Luis M. Schang
Summary: The 35th International Conference on Antiviral Research (ICAR) was held in Seattle, Washington, USA, on March 21-25, 2022, and via an interactive remote meeting platform. This report provides an overview of the conference, summarizing presentations and key conclusions from researchers in various areas of antiviral research and development. The conference showcased efforts and advancements in the field and demonstrated the significant response to the ongoing pandemic while emphasizing the importance of future pandemic preparedness.
ANTIVIRAL RESEARCH
(2023)
Article
Chemistry, Physical
Haoyue Xiang, Salvatore Ferla, Carmine Varricchio, Andrea Brancale, Nicola L. Brown, Gary W. Black, Nicholas J. Turner, Daniele Castagnolo
Summary: Two enzymatic strategies were developed to oxidize 1,2,3,4-tetrahydroquinolines (THQs) and N-cyclopropyl-N-alkylanilines into quinolines and 2-quinolones, respectively. Whole cells and monoamine oxidase (MAO-N) enzymes were used for the biotransformation of THQs, while horseradish peroxidase (HRP)-catalyzed annulation/aromatization reaction followed by Fe-mediated oxidation was employed for the conversion of N-cyclopropyl-N-alkylanilines into 2-quinolone compounds.
Article
Hematology
Omayra C. D. Liesdek, Roger E. G. Schutgens, Linda M. de Heer, Faiz Z. Ramjankhan, Silvie A. E. Sebastian, Joline J. Rohof, Willem J. L. Suyker, Rolf T. Urbanus
Summary: There is a change in platelet activation status after CF-LVAD implantation, which may contribute to the occurrence of complications such as thrombosis and hemorrhage.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Wobke E. M. van Dijk, Geke C. Poolen, Albert Huisman, Harry R. Koene, Rob Fijnheer, Noortje Thielen, Esther R. van Bladel, Karin P. M. van Galen, Roger E. G. Schutgens, Rolf T. Urbanus
Summary: This study found that patients with ITP were in a hypercoagulable state, with preactivated platelets and increased thrombin generation potential. Eltrombopag treatment further increased plasma thrombin generation potential but had no significant impact on other hemostatic parameters.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Engineering, Biomedical
Omayra C. D. Liesdek, Rolf T. Urbanus, Steven de Maat, Linda M. de Heer, Faiz Z. Ramjankhan, Silvie A. E. Sebastian, Albert Huisman, Nicolaas de Jonge, Aryan Vink, Kathelijn Fischer, Coen Maas, Willem J. L. Suyker, Roger E. G. Schutgens
Summary: Thrombus formation remains a common complication in LVAD therapy despite anticoagulation. This study found that LVAD patients had elevated levels of platelet protein, beta-thromboglobulin, and active von Willebrand factor, which remained high after LVAD implantation. High D-dimer levels and increased markers of coagulation and contact activation were observed after LVAD implantation. These findings suggest that LVAD patients experience significant coagulation activation and continuous platelet and contact activation, which may contribute to the high risk of thrombosis associated with LVAD.
Article
Hematology
S. Kretschmann, S. Voelkl, H. Reimann, G. Kroenke, G. Schett, S. Achenbach, G. Lutzny-Geier, F. Mueller, D. Mougiakakos, J. Dingfelder, C. Flamann, L. Hanssens, R. Gary, A. Mackensen, M. Aigner
Summary: Although the production of functional chimeric antigen receptor T cells has been demonstrated in patients with B-cell malignancies, it is uncertain whether sufficient amounts of functional autologous CAR T cells can be generated from patients with autoimmune diseases.
TRANSPLANTATION AND CELLULAR THERAPY
(2023)
Review
Chemistry, Medicinal
Luis Queiros-Reis, Joao R. Mesquita, Andrea Brancale, Marcella Bassetto
Summary: This review discusses the fatty acid binding pocket (FABP) in the spike glycoprotein of SARS-CoV-2 and its ligands with antiviral activity. Compounds that stabilize the inactive conformation of the spike protein and reduce viral infectivity show potential for the development of broad-spectrum antiviral agents.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Rheumatology
Felix Muehlensiepen, Susann May, Katharina Hadaschik, Nicolas Vuillerme, Martin Heinze, Manuel Grahammer, Hannah Labinsky, Sebastian Boeltz, Jacqueline Detert, Jana Petersen, Gerhard Kroenke, Georg Schett, Johannes Knitza
Summary: This study investigates the experiences, perceived drawbacks, and benefits of using an electronic patient-reported outcome (ePRO) web-app in supporting shared decision-making and treat-to-target approach in rheumatoid arthritis (RA) patients. The study finds that continuous ePRO collection and standardization can strain some patients, while certain ePRO may be outdated and difficult to understand. However, patients and physicians appreciate the improved overview of disease activity, capturing disease flares, and continuous remote monitoring. The use of ePRO is associated with paper and time savings. Physicians express concerns about becoming too focused on ePRO data, lack of reimbursement for ePRO monitoring, and app interoperability. Overall, the benefits of digitally supported shared decision-making with ePRO seem to outweigh observed drawbacks for RA patients and physicians.
RHEUMATOLOGY INTERNATIONAL
(2023)