Journal
SCIENCE CHINA-CHEMISTRY
Volume 60, Issue 12, Pages 1602-1608Publisher
SCIENCE PRESS
DOI: 10.1007/s11426-017-9061-1
Keywords
extraction kit; MBs@SiO2; lysis time; extraction efficiency
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Funding
- National Natural Science Foundation of China [61527806, 61471168, 61571187]
- China Post-doctoral Science Foundation [2016T90403]
- Economical Forest Cultivation and Utilization of Collaborative Innovation Center in Hunan Province [(2013)448]
- Project of Scientific Innovation Team of Ningbo [2015B11050]
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Nucleic acid extraction is one of the bases in molecular biology and it is the indispensable process for nucleic acid detection. Due to its excellent characteristics, the method based on magnetic beads has become one of the main methods for nucleic acid extraction. In this research, we have developed a rapid high-quality universal nucleic acid extraction kit based on magnetic beads (MBs) and named it MB-100. Some factors affecting nucleic acid extraction were optimized, such as particle size of MBs, surface groups of MBs and lysis time. Results demonstrated that micron-grade MBs@SiO2 particles were much more appropriate for nucleic acid extraction and lysis time was 5 min. Simultaneously, the performance of MB-100 was explored. Results showed that it had much higher extraction efficiency than other two commercial kits, and the following PCR reaction also had much higher amplification efficiency.
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