Article
Biotechnology & Applied Microbiology
Tanushree Chakraborty, K. Viswanatha Chaitanya, Maurizio Lambardi, Nasim Akhtar
Summary: This study developed a notable protocol for in-vitro regeneration of Pterocarpus santalinus and investigated the effect of plant growth regulators on callogenesis and morphogenesis. The results showed that a proper combination and concentration of growth regulators can significantly promote callus formation, shoot morphogenesis, and root development in P. santalinus.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Plant Sciences
Denis Okello, Sungyu Yang, Richard Komakech, Yuseong Chung, Endang Rahmat, Roggers Gang, Francis Omujal, Alice V. Lamwaka, Youngmin Kang
Summary: The study developed a protocol for the indirect regeneration of Aspilia africana and successfully achieved shoot organogenesis through leaf and root explants. The results showed that efficient callus induction and shoot regeneration capacity could be obtained through specific culture medium treatments, leading to the successful cultivation of regenerated plantlets with robust growth conditions.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemical Research Methods
Si Cheng Yao, Yuan Yuan Jiang, Su Ni, Long Wang, Jun Feng, Rui Wu Yang, Li Xia Yang, Qiu Yan Len, Li Zhang
Summary: S.m.-SC from Sichuan relies on root reproduction and zymad accumulation due to abnormal pollen development, with diseased plants showing typical viral disease symptoms and seed quality degeneration. The study detected tobacco mosaic virus and cucumber mosaic virus from mosaic-diseased plants, and established a highly efficient virus-free regeneration system to recover germplasm properties.
Article
Horticulture
Qianwen Tang, Xiangxin Guo, Yuanshan Zhang, Qingyun Li, Guanqun Chen, Huale Sun, Weiming Wang, Xiaohui Shen
Summary: A highly efficient protocol based on indirect organogenesis has been successfully established for Agapanthus praecox 'Big Blue', providing a practical reference for its application.
Article
Biotechnology & Applied Microbiology
C. P. Krishna Vrundha, T. Dennis Thomas
Summary: An efficient shoot regeneration protocol was developed for Thottea siliquosa via indirect shoot regeneration from root explants. The best callus induction and shoot induction responses were obtained with specific combinations of plant growth regulators. The field established in vitro propagated plants showed higher phytochemical content and antioxidant activity compared to the mother plants and root derived callus.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Biodiversity Conservation
Yuan-yuan Meng, Shi-jie Song, Sven Landrein
Summary: The study provides the first protocol for in vitro organogenesis and plant regeneration for Passiflora xishuangbannaensis, aiming at conservation and reintroduction efforts. Genetic stability of regenerated cells and analysis of genetic variation between the plantlets and the original plant were conducted. The response to plant growth regulators in this Asian species was found to be more specific, potentially due to differences in morphology and physiology between South American and Asian Passiflora species.
GLOBAL ECOLOGY AND CONSERVATION
(2021)
Review
Agronomy
Marzena Warchol, Edyta Skrzypek, Katarzyna Juzon-Sikora, Dragana Jakovljevic
Summary: Plant in vitro cultures are essential for improving crops and advancing plant biotechnology. They offer advantages over traditional breeding methods and can speed up the development of new cultivars. However, efficient methods for oat regeneration through in vitro culture are still lacking. This review provides an overview of the in vitro techniques used or potentially involved in oat breeding.
Article
Plant Sciences
Jothi Kanmani Bharathi, Ramaswamy Anandan, Srinivasan Rameshkumar, Kannan Menaka, Muthu Arjuna Samy Prakash
Summary: Costus speciosus is rapidly disappearing in its natural habitats in India due to non-selective collection and over-exploitation. In-vitro regeneration technology and the use of plant growth regulators have been successfully applied to propagate and root Costus speciosus, providing possibilities for commercial cultivation.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Plant Sciences
Wenhui Song, Yaohong Song, Xueting Liu, Xiaoju Zhang, Rujie Xin, Siyang Duan, Shixin Guan, Xiaomei Sun
Summary: In this study, an efficient in vitro system for callus induction and regeneration in herbaceous peony (Paeonia lactiflora Pall.) was developed by improving culture conditions and using plant growth regulators in the culture media. This system provides a foundation for subsequent genetic engineering research and enables rapid propagation and large-scale production of P. lactiflora.
Article
Plant Sciences
Ki-Beom Moon, Ji-Sun Park, Su-Jin Park, Hyo-Jun Lee, Hye-Sun Cho, Sung-Ran Min, Youn-Il Park, Jae-Heung Jeon, Hyun-Soon Kim
Summary: Efficient extraction of protoplasts and stable regeneration protocols are prerequisites for the continuous expansion and development of protoplast utilization systems. This study establishes an efficient process from protoplast isolation to in vitro shoot regeneration, achieving a significant increase in protoplast yield and successful shoot development.
Article
Horticulture
Yeganeh Basiri, Nematollah Etemadi, Mahdi Alizadeh, Javad Alizargar
Summary: In this study, an in vitro propagation technique for Eremurus spectabilis M. Bieb using tuberous root explants was established. The highest callus induction and shoot proliferation rates were observed on specific media compositions. The protocol can have commercial applications and be useful in the production of secondary metabolites.
Article
Plant Sciences
Carles Cortes-Olmos, Vladimir Marin Guerra-Sandoval, Vicente Blanca-Gimenez, Adrian Rodriguez-Burruezo
Summary: This study investigates the morphogenic potential of Gymnocalycium cv. Fancy through in vitro tissue culture techniques and successfully develops an efficient micropropagation protocol based on KIN and central discs as starting explants.
Article
Horticulture
Xia Chen, Chengyang Ye, Hongmin Yang, Wen Ji, Zhen Xu, Sanchun Ye, Huasen Wang, Songheng Jin, Chao Yu, Xiangtao Zhu
Summary: This study describes a protocol for high-frequency callus induction and establishment of peony plants using flower petals as explants. The effects of different medium compositions on the efficiency of the regeneration system were also investigated. The results showed that Murashige and Skoog (MS) medium supplemented with 2,4-D, 6-BA, and NAA was the best medium for callus induction, achieving an induction rate of up to 98.52%. Additionally, the surface structures of the calluses were observed using scanning electron microscopy, and it was found that different surface structures appeared to affect bud differentiation efficiency.
Article
Plant Sciences
Seung Yong Shin, Su-Jin Park, Hyun-Soon Kim, Jae-Heung Jeon, Hyo-Jun Lee
Summary: This study identified the importance of ROS and Ca2+ in de novo root organogenesis in detached leaves. It was found that ROS and Ca2+ have different functions in regulating this process, with ROS acting upstream of auxin signaling and Ca2+ acting downstream of auxin. Furthermore, it was observed that ROS and Ca2+ have little effect on auxin biosynthesis and transport in explants.
Article
Biochemistry & Molecular Biology
Abdul Kader, Sankar Narayan Sinha, Parthadeb Ghosh
Summary: Genetically stable plants of neem were produced through indirect organogenesis. Different explants cultivated on MS media with different growth regulators showed maximum callus formation. Optimized conditions resulted in the production of healthy plantlets with suitable proliferation and root induction media.
MOLECULAR BIOLOGY REPORTS
(2023)