4.1 Article

Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro

Publisher

SOC BRASILEIRA FARMACOGNOSIA
DOI: 10.1016/j.bjp.2016.12.004

Keywords

Flavonoid; Hyptis marrubioides; In vitro culture; Light quality

Funding

  1. CNPq [305783/2012-2]
  2. FAPESP [2011/00631-5]
  3. Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG) [201310267000257]
  4. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [11/00631-5] Funding Source: FAPESP

Ask authors/readers for more resources

Hyptis marrubioides Epling, Lamiaceae, a species from Brazilian Cerrado, has been used against gastrointestinal infections, skin infections, pain, and cramps. Herein, H. marrubioides seedlings were cultured in vitro under different wavelengths (white, blue, green, red, and yellow) with 50 mu mol m(-2) s(-1) irradiance and a 16-h photoperiod. After 20 and 30 days of cultivation, shoot length, leaf number, fresh mass, and dry mass were evaluated. The flavonoid rutin content was determined by the HPLC-DAD method. The shoots were longer in plants cultivated under yellow (16.603 +/- 0.790 cm, 1.8-fold), red (15.465 +/- 0.461 cm, 1.7-fold), and green (14.677 +/- 0.737 cm, 1.6-fold) lights than in control plants exposed to white light (9.203 +/- 0.388 cm). The number of leaves increased in plants exposed to red (23.425 +/- 1.138, 1.1-fold) and green (22.725 +/- 1.814, 1.1-fold) lights, compared to control plants (20.133 +/- 0.827). Fresh (0.665 +/- 0.048 g, 1.2-fold) and dry (0.066 +/- 0.005 g, 1.3-fold) mass of seedlings were the highest in seedlings grown under red light, compared to seedlings grown under white light (0.553 +/- 0.048 and 0.028 +/- 0.004, respectively). However, rutin production was higher under white (0.308 mg g(-1) of dry weight) and blue lights (0.298 mg g(-1) of dry weight). Thus, red light induces plant growth and increases leaf number and dry weight in in vitro-cultivated H. marrubioides, whereas blue and white lights promote the greatest rutin accumulation. (C) 2017 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.1
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available