Journal
RESEARCH IN MICROBIOLOGY
Volume 168, Issue 3, Pages 188-193Publisher
ELSEVIER
DOI: 10.1016/j.resmic.2016.11.002
Keywords
E. coli 0157; VBNC state; Cryopreservation; PMA-PCR; Toxin expression
Categories
Funding
- National 973-Plan of China [2012CB720800]
- National Natural Science Foundation of China [31201362, 31101278, 31501582]
- International Science & Technology Cooperation Program [2013B051000014]
- National Outstanding Doctoral Dissertadon Funding [201459]
- Guangdong Outstanding Doctoral Dissertation Funding [K3140030]
- Open Project Program of State Key Laboratory of Food Science and Technology, Jiangnan University [SKLF-KF-201513]
- Open Project Program of Key Laboratory for Green Chemical Process of Ministry of Education in Wuhan Institute of Technology [GCP201506]
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As major food-borne pathogens worldwide, Escherichia coli are capable of toxin production directly causing severe human disease. However, routine methods are incapable of detecting viable but non-culturable (VBNC) bacteria in food products and raw materials, leading to false negative identification. In this study, VBNC E. coil 0157 strains were acquired after cryopreservation at -20 degrees C, with and without freeze thawing; morphology was observed to be of shorter rod-shape, and toxin expression remained at relatively high levels. PMA-PCR assay for VBNC detection was also validated. Therefore, these results suggest that VBNC E. coil 0157 strains may represent a strong threat to public health and food safety. (C) 2016 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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