Journal
REPRODUCTIVE TOXICOLOGY
Volume 69, Issue -, Pages 19-33Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.reprotox.2017.01.003
Keywords
Oocyte; Cumulus cells; Gap junctions; Oocyte growth factors; Glucose metabolism; Environmental contaminants; Lifestyle factors
Categories
Funding
- Royal Society of New Zealand
- Marsden Fund [08-VUW-010, 13-VUW-153]
- Victoria University of Wellington PhD Scholarship
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Ovarian follicular fluid provides a potential reservoir for exogenous compounds that may adversely affect oocyte quality. This study examined the effects of common lifestyle and environmental contaminants, namely bisphenol-A (BPA), caffeine, 3,4-methylenedioxymethamphetamine (MDMA), nicotine and Delta 9-tetrahydrocannabinol (THC) on gap junction genes (Gja1, Gja4) and proteins (GJA1), glucose metabolism genes (Gfpt1, Pfkp) and oocyte growth factor genes (Bmp15, Gdf9), as well as gap junction transfer rate, in rat cumulus-oocyte complexes (COCs). In vitro exposure to MDMA and THC accelerated the timing of meiotic resumption and all contaminants altered either gap junction gene expression (BPA, caffeine, MDMA and THC) or transfer rate (BPA and nicotine). In vitro exposure of COCs to MDMA also altered glucose metabolism genes. Overall, oocyte-derived genes were largely unaffected following exposure to any contaminant. In summary, the impact of short-term exposure to lifestyle and environmental contaminants on oocyte function may be diminished due to protective properties of cumulus cells. (C) 2017 Elsevier Inc. All rights reserved.
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