Journal
REPRODUCTIVE BIOMEDICINE ONLINE
Volume 34, Issue 5, Pages 441-454Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rbmo.2017.02.001
Keywords
Group culture; IVF; Osmolality; Single culture; Stress; Time-lapse
Categories
Funding
- University of Melbourne
Ask authors/readers for more resources
Single embryo culture is suboptimal compared with group culture, but necessary for embryo monitoring, and culture systems should be improved for single embryos. Pronucleate mouse embryos were used to assess the effect of culture conditions on single embryo development. Single culture either before or after compaction reduced cell numbers (112.2 +/- 3.1; 110.2 +/- 3.5) compared with group culture throughout (127.0 +/- 3.4; P < 0.05). Reduction of media volume from 20 mu l to 2 mu l increased blastocyst cell numbers in single embryos cultured in 5% oxygen (84.4 +/- 3.2 versus 97.8 +/- 2.8; P < 0.05), but not in 20% oxygen (55.2 +/- 2.9 versus 57.1 +/- 2.8). Culture in microwell plates for the EmbryoScope and Primo Vision time-lapse systems changed cleavage timings and increased inner cell mass cell number (24.1 +/- 1.0; 23.4 +/- 1.2) compared with a 2 mu l microdrop (18.4 +/- 1.0; P < 0.05). Addition of embryo-conditioned media to single embryos increased hatching rate and blastocyst cell number (91.5 +/- 4.7 versus 113.1 +/- 4.4; P < 0.01). Single culture before or after compaction is therefore detrimental; oxygen, media volume and microwells influence single embryo development; and embryo-conditioned media may substitute for group culture. (C) 2017 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available