Journal
REPRODUCTION FERTILITY AND DEVELOPMENT
Volume 29, Issue 7, Pages 1447-1455Publisher
CSIRO PUBLISHING
DOI: 10.1071/RD16104
Keywords
adhesion; apoptosis; immune tolerance
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Funding
- National Natural Science Foundation of China [81571511, 81370711, 30901603]
- Doctor Foundation of Shandong Province [BS2009SW031]
- Science Foundation of Qilu Hospital of Shandong University
- Fundamental Research Funds of Shandong University [2015QLQN50, 2015QLMS24]
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GRIM-19 is associated with apoptosis, abnormal proliferation, immune tolerance and malignant transformation, and it also plays an important role in early embryonic development. Although the homologous deletion of GRIM-19 causes embryonic lethality in mice, the precise role of GRIM-19 in embryo implantation has not been elucidated. Here we show that GRIM-19 plays an important role in endometrial receptivity and embryo implantation. Day 1 to Day 6 pregnant mouse uteri were collected. Immunohistochemistry studies revealed the presence of GRIM-19 on the luminal epithelium and glandular epithelium throughout the implantation period in pregnant mice. The protein and mRNA levels of GRIM-19 were markedly decreased on Day 4 of pregnancy in pregnant mice, but there was no change in GRIM-19 levels in a group of pseudopregnant mice. Overexpression of GRIM-19 decreased the adhesion rate of RL95-2-BeWo co-cultured spheroids and increased apoptosis. Furthermore, STAT3 and IL-11 mRNA and protein levels were reduced by overexpressing GRIM-19, but protein and mRNA levels of TNF-alpha were increased. These findings indicate the involvement of GRIM-19 in the embryo implantation process by regulating adhesion, apoptosis and immune tolerance.
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