Article
Biochemistry & Molecular Biology
Robert Kohnke, Marcus Oliver Ahlers, Moritz Alexander Birkelbach, Florian Ewald, Michael Krueger, Imke Fiedler, Bjorn Busse, Max Heiland, Tobias Vollkommer, Martin Gosau, Ralf Smeets, Rico Rutkowski
Summary: This study investigated the chondroregenerative potency of intra-articular stem/stromal cell therapy in temporomandibular joint osteoarthritis. Results showed a significant increase in cartilage thickness in the stromal cell treated groups. Scanning electron microscopy detected a similar heterogeneity of mineralization and tissue porosity in the subchondral zone among all groups.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Pharmacology & Pharmacy
Andrew R. Champion, Sian Lewis, Stuart Davies, Dyfrig A. Hughes
Summary: Advanced Therapy Medicinal Products have the potential to transform care pathways, but with high costs and associated financial and healthcare risks, Wales is working to introduce these medicines to ensure patient access is maintained.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2021)
Review
Pharmacology & Pharmacy
Huw Lloyd-Williams, Dyfrig A. Hughes
Summary: Economic evaluations of advanced therapy medicinal products (ATMPs) have shown some evidence of cost-effectiveness, but are associated with significant uncertainty and bias. Products such as chimeric antigen receptor T-cell therapy, gene therapy, and tissue-engineered products have been identified as potentially cost-effective, but with limitations in long-term outcomes and evidence on health state utilities.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2021)
Article
Neurosciences
Charlotte Jaloux, Maxime Bonnet, Marie Vogtensperger, Marie Witters, Julie Veran, Laurent Giraudo, Florence Sabatier, Justin Michel, Regis Legre, Gaelle Guiraudie-Capraz, Francois Feron
Summary: A safer and more efficient manufacturing process for clinical grade olfactory stem cells has been developed and is expected to be used in a Phase I clinical trial involving autologous transplantation in digital nerves with neglected injury.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Cell Biology
Ilona Szablowska-Gadomska, Monika Humiecka, Joanna Brzezicka, Anna Chroscicka, Joanna Placzkowska, Tomasz Oldak, Malgorzata Lewandowska-Szumiel
Summary: Subcutaneous adipose tissue is a valuable source of ADSCs for use in ATMPs. Due to the short shelf-life and time constraints, sterility confirmation may not be possible before administration. Contamination was found in over 40% of lipoaspirates, but was successfully eliminated through additional monitoring and decontamination steps. Good manufacturing practices specific to ADSC-based ATMPs are necessary to obtain a sterile product.
Article
Cell & Tissue Engineering
Alexandra Norrick, Jasmina Esterlechner, Elke Niebergall-Roth, Ulf Dehio, Samar Sadeghi, Hannes M. Schroeder, Seda Ballikaya, Nicole Stemler, Christoph Ganss, Kathrin Dieter, Ann-Kathrin Dachtler, Patrick Merz, Saadettin Sel, James Chodosh, Claus Cursiefen, Natasha Y. Frank, Gerd U. Auffarth, Bruce Ksander, Markus H. Frank, Mark A. Kluth
Summary: This study developed a novel treatment strategy utilizing in vitro expanded allogeneic ABCB5(+) LSCs derived from human cadaveric limbal tissue. The product showed high percentages of cells expressing critical transcription factors for LSC stemness maintenance and corneal epithelial differentiation. Preclinical studies confirmed the engraftment potential of the cells and demonstrated their safety, leading to approval for a clinical trial to evaluate safety and therapeutic efficacy in patients with LSCD.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Immunology
Chiara Capelli, Carolina Cuofano, Chiara Pavoni, Simona Frigerio, Daniela Lisini, Sara Nava, Michele Quaroni, Valentina Colombo, Francesco Galli, Svetlana Bezukladova, Paola Panina-Bordignon, Giuseppe Gaipa, Patrizia Comoli, Giulio Cossu, Gianvito Martino, Andrea Biondi, Martino Introna, Josee Golay
Summary: Advanced Therapy Medicinal Products (ATMPs) based on somatic cells expanded in vitro, with or without genetic modification, is a rapidly growing area of drug development. Potency assays are crucial for assessing the quality of ATMPs, and biomarkers may serve as substitutes for complex functional potency tests in predicting the efficacy of these cell-based drugs in vivo.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Cell Biology
Beatriz Fernandez-Munoz, Ana Belen Garcia-Delgado, Blanca Arribas-Arribas, Rosario Sanchez-Pernaute
Summary: Neural stem cells are attractive for regenerative therapies and are being tested in clinical trials for neurological disorders. Different sources of neural stem cells have advantages and disadvantages, with ethical concerns surrounding embryonic sources. Further research is needed to improve efficiency and characterization of alternative sources.
Article
Cell Biology
Anna Labedz-Maslowska, Agnieszka Szkaradek, Tomasz Mierzwinski, Zbigniew Madeja, Ewa Zuba-Surma
Summary: This study optimized the protocols for processing and ex vivo expansion of adipose tissue-derived mesenchymal stem cells (AT-MSCs) for the development of an Advanced Therapy Medicinal Product (ATMP) for human use. Critical process parameters were identified and examined to ensure the quality of the final product containing AT-MSCs.
Review
Chemistry, Analytical
Hojjatollah Nazari, Asieh Heirani-Tabasi, Sadegh Ghorbani, Hossein Eyni, Sajad Razavi Bazaz, Maryam Khayati, Fatemeh Gheidari, Keyvan Moradpour, Mousa Kehtari, Seyed Mohsen Ahmadi Tafti, Seyed Hossein Ahmadi Tafti, Majid Ebrahimi Warkiani
Summary: Microfluidics is a promising approach for the fabrication of monodispersed droplets, offering potential solutions to the limitations of regenerative medicine. This technology has been applied in various fields such as stem cell therapy, tissue engineering, reproductive biology, and gene therapy.
Review
Biochemistry & Molecular Biology
Matin Chehelgerdi, Fereshteh Behdarvand Dehkordi, Mohammad Chehelgerdi, Hamidreza Kabiri, Hosein Salehian-Dehkordi, Mohammad Abdolvand, Sharareh Salmanizadeh, Mohsen Rashidi, Anoosha Niazmand, Saba Ahmadi, Sara Feizbakhshan, Saber Kabiri, Nasimeh Vatandoost, Tayebeh Ranjbarnejad
Summary: The emergence of iPSCs has revolutionized stem cell research and opened up new avenues for cancer treatment. iPSCs provide a unique platform for investigating the origin of cancer and can be used for personalized treatment and drug testing. This review article discusses the science behind iPSCs, the limitations and challenges in iPSC-based cancer therapy, ethical and social implications, and a comparative analysis with other stem cell types for cancer treatment. It also explores the applications of iPSCs in tumorigenesis and the future of iPSC research in this field.
Review
Cell & Tissue Engineering
Qinjun Zhao, Zhibo Han, Jialun Wang, Zhongchao Han
Summary: The regulations for clinical application of MSCs therapy in China have been updated in recent years, with increasing efforts from biotech companies in developing MSC products. Discussions and research on MSC regulation have become hot topics in professional journals.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Review
Cardiac & Cardiovascular Systems
Doris A. Taylor, Lourdes Chacon-Alberty, Luiz C. Sampaio, Mariana Gonzalez Del Hierro, Emerson C. Perin, Fernanda C. P. Mesquita, Timothy D. Henry, Jay H. Traverse, Carl J. Pepine, Joshua M. Hare, Michael P. Murphy, Phillip C. Yang, Keith L. March, Rachel W. Vojvodic, Ray F. Ebert, Roberto Bolli
Summary: Cell-based therapy lacks standardized definitions and terminology, making comparisons across studies challenging. This article discusses the lack of consistency in descriptive terminology used in cell-based therapies and provides guidelines for standardizing nomenclature and definitions.
CARDIOVASCULAR RESEARCH
(2022)
Review
Cell Biology
Mohammad Amin Shahrbaf, Masoumeh Nouri, Morteza Zarrabi, Roberto Gramignoli, Massoud Vosough
Summary: Liver disorders are on the rise globally, placing a significant burden on healthcare systems. While whole organ transplantation has been the primary treatment option for liver diseases, recent developments in cell-based therapies offer promising alternatives. Extraembryonic tissues contain multipotent stem cells that show robust anti-inflammatory and anti-fibrotic properties, making them potentially effective in managing liver fibrosis, cirrhosis, and chronic inflammatory conditions.
Editorial Material
Medicine, Research & Experimental
Jose Maria Lamo-Espinosa, Felipe Prosper, Juan F. Blanco, Fermin Sanchez-Guijo, Mercedes Alberca, Veronica Garcia, Margarita Gonzalez-Vallinas, Javier Garcia-Sancho
Summary: The study found that using 10, 40, and 100 million autologous cells for knee osteoarthritis treatment provided similar and long-lasting results, providing strong evidence for the efficacy of single-cell injection therapy.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Medicine, General & Internal
Enrique Gomez-Barrena, Norma G. Padilla-Eguiluz, Philippe Rosset, Philippe Hernigou, Nicola Baldini, Gabriela Ciapetti, Rosa M. Gonzalo-Daganzo, Cristina Avendano-Sola, Helene Rouard, Rosaria Giordano, Massimo Dominici, Hubert Schrezenmeier, Pierre Layrolle
Summary: The study demonstrated the safety and early efficacy of treating early femoral head osteonecrosis with autologous mesenchymal stromal cells (MSC) expanded from bone marrow. Results showed that 80% of patients maintained head sphericity and showed bone regeneration at 2 years, with 19% converting to total hip replacement (THR) after 5 years.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Meadhbh A. Brennan, Mario Barilani, Francesco Rusconi, Julien de Lima, Luciano Vidal, Cristiana Lavazza, Lorenza Lazzari, Rosaria Giordano, Pierre Layrolle
Summary: BMSCs demonstrate superior osteogenic potential in vitro, while CBMSCs show better chondrogenic differentiation and secretion of interleukins. Although BMSCs exhibit higher cell engraftment and ectopic bone formation compared to CBMSCs in vivo, priming CBMSCs with supplements can also induce bone formation.
SCIENTIFIC REPORTS
(2021)
Review
Oncology
Araceli Rivera-Ordaz, Valeria Peli, Paolo Manzini, Mario Barilani, Lorenza Lazzari
Summary: The importance of designing the manufacturing process of hiPSCs using quality-by-design principles is emphasized in the article, highlighting the critical role of cell quantity, quality, and costs in bioreactor systems.
Article
Cell & Tissue Engineering
Chiara Capelli, Simona Frigerio, Daniela Lisini, Sara Nava, Giuseppe Gaipa, Daniela Belotti, Benedetta Cabiati, Silvia Budelli, Lorenza Lazzari, Jessica Bagnarino, Matteo Tanzi, Patrizia Comoli, Norberto Perico, Martino Introna, Josee Golay
Summary: This report aims to provide evidence-based information for stability studies on ATMPs and promote harmonization of practices among laboratories. Through the analysis of stability studies on 19 different cell-based experimental ATMPs, it was found that these cell products are highly stable when stored at low temperatures for an extended period and show no decreased viability or efficacy. The report suggests the development of new guidelines specific to ATMPs to reduce the costs of stability studies.
Article
Cell Biology
Mario Barilani, Christopher Lovejoy, Roberta Piras, Andrey Y. Abramov, Lorenza Lazzari, Plamena R. Angelova
Summary: The study found that aging in MSCs is associated with changes in mitochondrial metabolism, including a decrease in mitochondrial membrane potential, lower NADH levels, and higher mitochondrial DNA content. Despite higher ATP levels in aged cells, increased rates of mitophagy lead to decreased overall mitochondrial mass. Additionally, changes in mitochondrial metabolism in aged MSCs result in the overproduction of reactive oxygen species in mitochondria, which is compensated by higher levels of the endogenous antioxidant glutathione.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Medicine, Research & Experimental
Cristiana Lavazza, Silvia Budelli, Elisa Montelatici, Mariele Vigano, Francesca Ulbar, Lucia Catani, Marta Giulia Cannone, Sara Savelli, Elisa Groppelli, Lorenza Lazzari, Roberto M. Lemoli, Matteo Cescon, Gaetano La Manna, Rosaria Giordano, Tiziana Montemurro
Summary: This study validates a Good Manufacturing Practice (GMP)-compliant protocol for the production of T-reg cells, which enables the generation of a sufficient number of functional T-reg cells with immunomodulatory properties. These results are important for implementing cell therapy protocols aimed at restoring T-reg cell number and function, and slowing the progression of certain diseases.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Alessandro Cherubini, Francesco Rusconi, Lorenza Lazzari
Summary: The widespread use of organoids, a new three-dimensional culture technology, in scientific research has increased significantly in recent years due to their plasticity and multiple applications. This study focuses on human pancreatic organoids (hPOs) and compares 12 well-known housekeeping genes for accurate molecular analysis. The importance of selecting a stable reference gene for RT-qPCR data normalization in organoid studies is emphasized to ensure accurate and reproducible results.
Article
Neurosciences
Rosaria Giordano, Margherita Canesi, Maurizio Isalberti, Giovanni Marfia, Rolando Campanella, Daniele Vincenti, Viviana Cereda, Alessandra Ranghetti, Chiara Palmisano, Ioannis Ugo Isaias, Riccardo Benti, Giorgio Marotta, Lorenza Lazzari, Tiziana Montemurro, Mariele Vigano, Silvia Budelli, Elisa Montelatici, Cristiana Lavazza, Araceli Rivera-Ordaz, Gianni Pezzoli
Summary: This study assessed the safety of MSC treatment in patients with PSP and identified critical issues in cell therapies for neurodegenerative diseases. The results showed that MSC treatment did not have a significant impact on 1-year survival rate and occurrence of adverse events in most treated patients.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Medicine, General & Internal
Lorenzo Lazzari, Chiara Oltolini, Fabio Ciceri, Fabio Giglio
Summary: This case report demonstrates the typical clinical course of COVID-19 in severely immunosuppressed patients and emphasizes the importance of prompt treatment directed towards viral clearance to compensate for the absence of valid immune reactivity in this population.
Article
Biotechnology & Applied Microbiology
Valeria Peli, Mario Barilani, Araceli Rivera-Ordaz, Paolo Manzini, Francesco Rusconi, Cristiana Lavazza, Silvia Cimoni, Lorenza Lazzari
Summary: This study validated an image cytometer-based method for determining the viability of human induced pluripotent stem cells (hiPSCs) and demonstrated its feasibility as a quality control test during large-scale GMP hiPSC expansion.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Multidisciplinary Sciences
Katia Chieregato, Martina Bernardi, Alberta Alghisi, Rosaria Giordano, Lorenza Lazzari, Omar Perbellini, Mario Rassu, Marco Ruggeri, Giuseppe Astori
Summary: Mesenchymal stromal cells (MSC) have potential in treating aGvHD and autoimmune disorders, but their recognition mechanisms are unclear and they are not completely immunoprivileged. Developing an in vitro test and a flow cytometric assay may be an easy method for predicting treatment response.
