Review
Biochemistry & Molecular Biology
Gemma Arderiu, Anna Civit-Urgell, Lina Badimon
Summary: The incidence and prevalence of critical limb ischemia have increased, but there are no successful treatments available. Adipose-derived stem cells (ASCs) are a promising source of stem cells for cell-based therapy due to their multilineage capacity and low immunogenicity. The proangiogenic benefits of ASCs are attributed to their secretion of proangiogenic molecules, microvesicles/exosomes, and their differentiation capability toward endothelial cells (ECs). However, the therapeutic efficacy of ASCs in peripheral artery diseases patients is still relatively low.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Georgiana Panci, Benedicte Chazaud
Summary: Adult skeletal muscle fully regenerates after injury through muscle stem cells following the myogenic program, with immune cells like leukocytes playing a crucial role in the coordinated actions for regeneration. Leukocytes interact not only with muscle stem cells but also with other cell types, impacting skeletal muscle regeneration.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yundong Peng, Jingjing Du, Stefan Gunther, Xinyue Guo, Shengpeng Wang, Andre Schneider, Li Zhu, Thomas Braun
Summary: Skeletal muscle stem cells, also known as satellite cells, play a crucial role in postnatal muscle growth and regeneration. This study reveals that Piezo1, a mechanosensitive ion channel, is essential for maintaining the quiescence and preventing senescence of muscle stem cells. Absence of Piezo1 leads to premature activation of these cells, impairs their proliferation and differentiation, and significantly hinders skeletal muscle regeneration. The inactivation of Piezo1 results in compensatory up-regulation of T-type voltage-gated Ca2+ channels, which increases the influx of Ca2+ and induces NOX4 expression through cPKC. The elevated expression of NOX4 in Piezo1-deficient cells leads to increased levels of reactive oxygen species (ROS) and DNA damage, causing P53-dependent cellular senescence and cell death. The study also highlights the importance of the P53/P21-axis in mediating the cellular defects caused by the absence of Piezo1. This research sheds light on the critical role of Piezo1-mediated mechano-signaling in maintaining the quiescence and preventing senescence of muscle stem cells, and suggests that reduced mechano-signaling during aging may contribute to the accumulation of senescent cells and the decline of muscle stem cell numbers in geriatric individuals.
Article
Biochemistry & Molecular Biology
Ivan J. Vechetti, Bailey D. Peck, Yuan Wen, R. Grace Walton, Taylor R. Valentino, Alexander P. Alimov, Cory M. Dungan, Douglas W. Van Pelt, Ferdinand von Walden, Bjorn Alkner, Charlotte A. Peterson, John J. McCarthy
Summary: Regular physical activity may improve health by releasing factors from skeletal muscle through extracellular vesicles (EVs) containing miR-1, promoting adipose tissue metabolic adaptations. EVs are preferentially taken up by eWAT, enhancing adrenergic signaling and lipolysis, ultimately benefiting overall health.
Article
Developmental Biology
Ryo Fujita, Solene Jamet, Graham Lean, Harry Chun Man Cheng, Steven Hebert, Claudia L. Kleinman, Colin Crist
Summary: Translation control of gene expression through P-eIF2α enables the expansion of satellite cells. Certain genes are upregulated at the protein level without corresponding changes at the mRNA level. Deficiency of Tacc3 results in defects in the expansion, self-renewal, and regeneration of skeletal muscle in satellite cells.
Review
Cell Biology
Gaetan Juban, Benedicte Chazaud
Summary: Efferocytosis, the engulfment of dead cells by macrophages, plays a crucial role in tissue repair post-injury by delineating the transition from the pro-inflammatory phase to the recovery phase. In the context of skeletal muscle regeneration, efferocytosis is essential for sterile tissue injury followed by complete regeneration, with molecular mechanisms and metabolic control playing a significant role in this process.
Article
Biochemistry & Molecular Biology
Sergio Perez-Diaz, Zeynab Koumaiha, Matthew Jay Borok, Frederic Aurade, Maria Pini, Baptiste Periou, Christine Rouault, Yasmine Baba-Amer, Karine Clement, Genevieve Derumeaux, Francois Jerome Authier, Peggy Lafuste, Frederic Relaix
Summary: Obesity leads to skeletal muscle physio-pathological alterations, and the crosstalk between adipose tissue and myogenic cells plays a key role in this process. NID-1, secreted by skeletal muscle fibro-adipogenic/mesenchymal progenitors (FAPs), impairs myogenic potential and promotes excessive deposition of extracellular matrix, thereby mediating the alteration of myogenic function by adipose tissue.
Article
Multidisciplinary Sciences
Yasutomo Miura, Mase Sato, Toshie Kuwahara, Tomoki Ebata, Yasuhiko Tabata, Hidetoshi Sakurai
Summary: Duchenne muscular dystrophy (DMD) is a challenging genetic muscular disorder characterized by the loss of DYSTROPHIN. Researchers have explored the transplantation of iMuSCs into the diaphragm as a potential therapeutic method to improve respiratory function in DMD patients. The study showed promising results in improving the engraftment of transplanted cells with the use of an injectable hyaluronic acid-gelatin solution in the diaphragm.
Review
Biochemistry & Molecular Biology
Rachel J. Waldemer-Streyer, Dongwook Kim, Jie Chen
Summary: Regeneration of adult skeletal muscle in mammals is a complex process regulated by multiple proteins and signaling pathways. Cytokines play a crucial role in skeletal myogenesis, with both infiltrating immune cells and the muscle itself secreting these signaling molecules. This review focuses on muscle-secreted cytokines that have been shown to be involved in myogenic differentiation and muscle regeneration.
Article
Biochemistry & Molecular Biology
Clara Bernard, Charline Jomard, Benedicte Chazaud, Julien Gondin
Summary: Post-injury skeletal muscle regeneration requires interactions between myogenic and non-myogenic cells. The kinetics of changes in muscle stem cells, endothelial cells, fibro-adipogenic progenitors, and macrophages in the regenerating muscle were similar in mild and severe injury models. However, the magnitude of changes in the number of differentiating muscle stem cells, hematopoietic cells, and fibro-adipogenic progenitors was higher in severe muscle damage.
Article
Multidisciplinary Sciences
Konxhe Kulaj, Alexandra Harger, Michaela Bauer, Oezuem S. Caliskan, Tilak Kumar Gupta, Dapi Menglin Chiang, Edward Milbank, Josefine Reber, Angelos Karlas, Petra Kotzbeck, David N. Sailer, Francesco Volta, Dominik Lutter, Sneha Prakash, Juliane Merl-Pham, Vasilis Ntziachristos, Hans Hauner, Michael W. Pfaffl, Matthias H. Tschoep, Timo D. Mueller, Stefanie M. Hauck, Benjamin D. Engel, Jantje M. Gerdes, Paul T. Pfluger, Natalie Krahmer, Kerstin Stemmer
Summary: Extracellular vesicles (EVs) derived from adipocytes transfer insulinotropic proteins to pancreatic β-cells, enhancing insulin secretion and glucose tolerance. This process is observed in insulin-resistant obese mice, suggesting that AdEVs play a role in informing β-cells about insulin resistance and increasing insulin demand.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Pin-yi Liu, Hui-qin Li, Meng-qi Dong, Xin-ya Gu, Si-yi Xu, Sheng-nan Xia, Xin-yu Bao, Yun Xu, Xiang Cao
Summary: Properdin activates Mincle receptors on microglia to drive inflammatory responses, exacerbating brain injury in ischemic stroke patients. The interaction between properdin and Mincle on microglia may be a potential therapeutic target for improving ischemic stroke prognosis.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Biochemistry & Molecular Biology
Giorgio Caratti, Thibaut Desgeorges, Gaetan Juban, Ulrich Stiffel, Aurelie Fessard, Mascha Koenen, Bozhena Caratti, Marine Theret, Carsten Skurk, Benedicte Chazaud, Jan P. Tuckermann, Remi Mounier
Summary: AMP-activated protein kinase (AMPK) plays a crucial role in muscle regeneration and resolution of inflammation in acute lung injury, and mediates the effects of glucocorticoids (GCs) on macrophages.
Review
Medicine, General & Internal
Aoxuan Zhu, Na Liu, Yujia Shang, Yonghuan Zhen, Yang An
Summary: This review provides insights into the potential mechanism of adipose stem cell-derived exosomes (ASC-exos) in muscle regeneration. The authors discuss various signaling pathways, such as Wnt, MAPK, PI3K/Akt, and JAK/STAT, and how ASC-exos contribute to different stages of skeletal muscle regeneration through these pathways.
CHINESE MEDICAL JOURNAL
(2022)
Article
Cell & Tissue Engineering
R. El-Habta, G. Andersson, P. J. Kingham, L. J. Backman
Summary: This study investigates the effects of stromal vascular fraction (SVF) on muscle cell apoptosis following denervation. Results show that SVF can significantly reduce apoptosis in denervated muscles, both in vitro and in vivo, thereby preserving muscle tissue and potentially providing therapeutic benefits for muscle nerve injuries.
STEM CELL RESEARCH & THERAPY
(2021)
