Journal
ENVIRONMENTAL ENTOMOLOGY
Volume 44, Issue 2, Pages 418-425Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/ee/nvv010
Keywords
Reference gene; UVB; RT-qPCR; Tribolium castaneum
Categories
Funding
- National Natural Science Foundation of China [31171856]
- China Scholarship Council
- HuaZhong Agriculture University
Ask authors/readers for more resources
Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) has become a widely used technique to quantify gene expression. It is necessary to select appropriate reference genes for normalization. In the present study, we assessed the expression stability of seven candidate genes in Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) irradiated by ultraviolet B (UVB) at different developmental stages for various irradiation time periods. The algorithms of geNorm, NormFinder, and BestKeeper were applied to determine the stability of these candidate genes. Ribosomal protein genes RpS3, RpL13A, and beta-actin gene (ActB) showed the highest stability across all UVB irradiation time points, whereas expression of other normally used reference genes, such as those encoding the beta-tubulin gene TUBB and the E-cadherin gene CAD, varied at different developmental stages. This study will potentially provide more suitable reference gene candidates for RT-qPCR analysis in T. castaneum subjected to environmental stresses, particularly UV irradiation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available