Article
Biotechnology & Applied Microbiology
Shaoxing Chen, Siqi Sun, Rui Wang, Hongli Feng, Hua Xiang
Summary: Halolysin R4 from Haloferax mediterranei provides its host antagonistic and defensive activities against other haloarchaea, expanding our knowledge of the traditional function of haloarchaeal extracellular proteases. This protein may be secreted independently of growth phase, lysing competing cells in the same environment and utilizing cell debris as nutrients, potentially playing a crucial role in nutrient cycling in extremely hypersaline environments.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ramanjeet Kaur, Santosh Kumar Tiwari
Summary: Halocins are antimicrobial peptides secreted by haloarchaea, with Halocin HA4 being a heat-resistant compound with unique N-terminal sequence. It retains complete activity in the presence of various organic compounds, but its activity is reduced after treatment with certain enzymes, indicating its proteinaceous nature. Halocin HA4's cytocidal nature was demonstrated by its interaction with cell membrane and nucleic acids of target cells, suggesting potential applications in food preservation and leather industries.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Chemistry, Analytical
Seonjeong Lee, Shinyeong Ju, Seok Jin Kim, Jin-Oh Choi, Kihyun Kim, Darae Kim, Eun-Seok Jeon, Cheolju Lee
Summary: tipNrich is an optimized N-terminal analysis method for analyzing small amounts of protein samples, allowing for rapid assessment of protein quality or characterization of N-terminal proteoform-related diseases.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Anne Susemihl, Felix Nagel, Piotr Grabarczyk, Christian A. Schmidt, Mihaela Delcea
Summary: Zinc finger proteins play important roles in various cellular processes, with BCL11B being a member of this protein family. A simple and fast protocol was described for preparing a fluorescently tagged protein for in vitro studies, which can be valuable for further research in understanding zinc finger domains.
Article
Biotechnology & Applied Microbiology
Xiaomei He, Shuncheng Zhang, Dongya Dang, Tingting Lin, Yuanyuan Ge, Xiaofeng Chen, Jun Fan
Summary: This study optimized the separation conditions for the hanA1-tagged EmGFP protein and successfully used five different colored proteins to detect the effectiveness of the hanA1 fusion handle. The results suggest that the fusion hanA1 tag could be potentially used for simple and cheap affinity separation of target proteins in industry and diagnosis.
MICROBIAL CELL FACTORIES
(2023)
Article
Biochemistry & Molecular Biology
Veronique Jonckheere, Petra Van Damme
Summary: The study identified a novel proteoform of the evolutionary conserved N-alpha acetyltransferase Naa40p, named Naa40p25, and revealed the differences in cellular localization, translation function, and protein interactions between this proteoform and the canonical Naa40(L) form; Additionally, two new Naa40p substrates were discovered, and Lge1 was confirmed as the first redundant substrate for yNatA and yNatD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Chang Ho Ri, Song Ryong Li, Chon Il Paek, Yu Sin Kim
Summary: From a haloarchaeal strain isolated from salt-fermented shrimp, a pure 0.32 mg halocin with 96% purity was obtained. Through fusion with glutathione-S-transferase (GST) in E. coli and affinity purification followed by enterokinase (EK) cleavage, a 6.2 mg recombinant HalC8 with 95% purity was obtained. The recombinant HalC8 showed the same molecular weight, folding pattern, and inhibitory activity against Haloferax volcanii as the native HalC8.
Editorial Material
Cell Biology
Lan Jiang, Tianyun Shen, Xinyuan Wang, Lunzhi Dai, Kefeng Lu, Huihui Li
Summary: Posttranslational modification plays a crucial role in regulating protein functions. The functions and mechanisms of N-terminal acetylation, which occurs on the first amino acids of proteins, in the field of macroautophagy/autophagy are not well understood. Recent studies have shown that the N-terminal acetyltransferase NatB is essential for autophagy and it modifies actin cytoskeleton constituent Act1 and dynamin-like GTPase Vps1, promoting the formation and fusion of autophagosomes.
Review
Biochemistry & Molecular Biology
Alka Rani, Khem Chand Saini, Felix Bast, Sunita Varjani, Sanjeet Mehariya, Shashi Kant Bhatia, Neeta Sharma, Christiane Funk
Summary: This review summarizes the research on antimicrobial molecules from microorganisms, briefly describing potential antimicrobial compounds from different types of microorganisms and highlighting the possibility of using microorganisms as a resource for antimicrobial applications. However, it also points out the need for further investigation to isolate, purify, and characterize these bioactive compounds in order to develop clinically approved antibiotics.
Article
Biochemistry & Molecular Biology
Phong Quoc Nguyen, Christine Conesa, Elise Rabut, Gabriel Bragagnolo, Celia Gouzerh, Carlos Fernandez-Tornero, Pascale Lesage, Juan Reguera, Joel Acker
Summary: Long-terminal repeat (LTR) retrotransposons replicate by reverse transcription of an RNA intermediate into a complementary DNA (cDNA) that is integrated into the host genome by their integrase. Recombinant Ty1 integrase, highly expressed in E.coli, efficiently catalyzes concerted integration in vitro, providing potential insights into the molecular mechanisms of Ty1-specific integration.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Shaomin Lin, Chunmao He
Summary: In this study, a simplified purification strategy for rubredoxins based on RP-HPLC is proposed, which allows for high yield and high resolution after just one-step purification. The RP-HPLC technique is also shown to be a valuable tool for investigating the thermal decomposition pathway of Pf-Rds.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Itschak Axelrad, Mary Safrin, Rivka Cahan, Sang-Jin Suh, Dennis E. Ohman, Efrat Kessler
Summary: Pseudomonas aeruginosa secretes multiple endopeptidases, with LysC identified as the principal activating protease for pro-PaAP. Elastase and Apr indirectly participate in the activation of PaAP, and the N-terminal domain of AP56 may regulate hydrolysis of natural peptides/proteins.
News Item
Genetics & Heredity
Chang Huang, Kristian Helin
Summary: New research suggests that histone H2B N terminus multisite lysine acetylation (H2BNTac) is a significant characteristic of active enhancers and could greatly enhance enhancer prediction accuracy.
Article
Virology
Zaloa Aguirre Sourrouille, Sabine Schwarzer, Sebastian Lequime, Hanna M. Oksanen, Tessa E. F. Quax
Summary: Viruses can infect members of all three domains of life, but little is known about viruses infecting archaea and their mechanisms of host interactions. In this study, researchers tested nearly 100 haloarchaeal virus isolates and identified 10 isolates capable of infecting Haloferax strains, with only one strain being susceptible to all 10 viruses. Comparative genomics was used to shed light on the factors determining the host range of haloarchaeal viruses on Haloferax.
Article
Biochemistry & Molecular Biology
Matthea R. Sanderson, Richard P. Fahlman, Rachel Wevrick
Summary: MAGEL2 protein may form biomolecular condensates through liquid-liquid phase separation, potentially involved in RNA metabolism and regulation of mRNAs modified by m(6)A methylation. The study provides mechanistic insights into pathogenic MAGEL2 mutations associated with Schaaf-Yang syndrome and related disorders.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Microbiology
Vijay Kumar, Jyoti Saxena, Santosh Kumar Tiwari
ARCHIVES OF MICROBIOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Vijay Kumar, Poonam Sheoran, Aabha Gupta, Jayaparkash Yadav, Santosh Kumar Tiwari
ANNALS OF MICROBIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Manoj Kumar Yadav, Vijay Kumar, Bijender Singh, Santosh Kumar Tiwari
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Vijay Kumar, Santosh Kumar Tiwari
Review
Biotechnology & Applied Microbiology
Vijay Kumar, Bijender Singh, Marco J. van Belkum, Dzung B. Diep, Michael L. Chikindas, Alexey M. Ermakov, Santosh Kumar Tiwari
Summary: Haloarchaea have evolved to survive under extreme saline conditions by accumulating osmolytes and salts, resulting in proteins that remain active at high ionic strength. Halocins, which are antimicrobial substances produced by haloarchaea, are stable under extreme conditions and have potential biotechnological applications. Despite limited knowledge on their genetics, biosynthetic pathways, and mechanism of action, there is growing interest in exploring the therapeutic and food preservation applications of halocins.
BIOTECHNOLOGY ADVANCES
(2021)