Article
Plant Sciences
Maolong Hu, Hongkun Zhang, Lingna Kong, Juanjuan Ma, Ting Wang, Xinyu Lu, Yue Guo, Jiefu Zhang, Rongzhan Guan, Pu Chu
Summary: The application of herbicides is an effective strategy for weed control, and the development of herbicide-resistant crops can enhance weed management. However, the use of the herbicide tribenuron-methyl (TBM) is limited in rapeseed fields due to rapeseed's sensitivity to TBM. A study was conducted on the TBM-resistant rapeseed mutant M342 and its wild-type plants, revealing that M342 exhibited improved tolerance to TBM and had higher levels of proteins related to non-target-site resistance (NTSR) to herbicides compared to the wild-type plants. Differentially accumulated proteins (DAPs) between the two genotypes were enriched in glutathione metabolism and oxidoreduction coenzyme metabolic process, providing protection against oxidative stress caused by TBM. These findings offer insights into the mechanism of NTSR in plants and contribute to the development of herbicide-resistant crops.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Caiyue Liu, Tianbao Zhang, Xinsen Yang, Liu Wang, Yan Long, Agula Hasi, Xinwu Pei
Summary: In this study, a mutant with resistance to ALS-inhibiting herbicides was obtained from the elite cultivar Longya10 through EMS mutagenesis. The mutant showed significantly increased resistance to TBM and slight resistance to imazethapyr compared to Longya10. Biochemical analysis revealed a Pro197Ser mutation in the LuALS1 gene, which conferred high resistance to sulfonylurea herbicides in the mutant. Two CAPS markers were developed based on this mutation site for marker assistant selection in breeding. The mutant did not cause yield losses in natural field conditions, making it a valuable germplasm resource for breeding herbicide-resistant flax varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Lei Chen, Gang Gu, Chengxu Wang, Zhufeng Chen, Wei Yan, Man Jin, Gang Xie, Junli Zhou, Xing Wang Deng, Xiaoyan Tang
Summary: Herbicide resistance is valuable for integrated weed management in agriculture, particularly in rice fields where weedy rice poses a challenge. This study obtained three mutant variants of the OsALS gene in rice through ethyl methyl sulfonate mutagenesis, with W-548 M mutant showing high levels of resistance to a broad spectrum of herbicides. The stable inheritance of herbicide resistance in rice lines makes W-548 M mutation a valuable resource for breeding herbicide resistant rice and weed management.
Article
Plant Sciences
Shmuel Galili, Joseph Hershenhorn, Marvin Edelman, Vladimir Sobolev, Evgeny Smirnov, Orit Amir-Segev, Aharon Bellalou, Evgenia Dor
Summary: Chickpea is an important crop in Israel, but sensitive to imidazolinone herbicides. A chickpea line resistant to these herbicides was developed using chemical mutagenesis, with a point mutation detected in one of the genes encoding the AHAS catalytic subunit. The mutation provided resistance to a specific group of herbicides and showed single-gene semi-dominant inheritance pattern, as confirmed by genetic transformation in tobacco plants.
Article
Biology
Rui Zhang, Sha Chen, Xiangbing Meng, Zhuangzhuang Chai, Delin Wang, Yuge Yuan, Kunling Chen, Linjian Jiang, Jiayang Li, Caixia Gao
Summary: Utilizing genome editing, various missense mutations were created in rice to confer tolerance to different herbicides, with a novel triple mutant showing high tolerance to all tested herbicides. Field trials suggested that these mutated varieties have potential practical applications in weed control.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Agronomy
Axelle Koreki, Severine Michel, Caroline Lebeaux, Lidwine Trouilh, Christophe Delye
Summary: This study investigates the prevalence, mode of evolution, and spread of resistance-endowing ALS alleles in corn poppy populations in France. With the use of massively parallel amplicon sequencing, it was found that mutant ALS alleles have complex geographic distributions, which could be attributed to large population sizes, multiple independent mutation events, and human-mediated spread of resistance. The study also reveals that mutant ALS alleles are associated with resistance to the synthetic auxin 2,4-D.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Yi Cao, Xinxin Zhou, Zhaofeng Huang
Summary: The study revealed the molecular basis for resistance of Amaranthus retroflexus to ALS-inhibiting herbicides, with Trp-574-Leu and Gly-654-Tyr substitutions in ALS contributing to broad-spectrum resistance. The Gly-654-Tyr substitution, previously unreported, conferred high resistance to multiple classes of ALS-inhibiting herbicides in the R-Tyr654 population. Despite this resistance, effective control of the population was achieved with herbicides targeting other sites of action.
PEST MANAGEMENT SCIENCE
(2022)
Article
Plant Sciences
Ryan Widianto, Denny Kurniadie, Dedi Widayat, Uum Umiyati, Ceppy Nasahi, Santika Sari, Abdul Shukor Juraimi, Hisashi Kato-Noguchi
Summary: Monochoria vaginalis is an aquatic herbaceous plant that is often found as a noxious weed in paddy fields. This research evaluated the resistance levels of M. vaginalis to different groups of herbicides and investigated the mutations in the ALS gene of the resistant biotype. The study found that the resistant biotype had varying levels of resistance to different herbicides and had specific amino acid substitutions in the ALS protein. Further research is needed to understand the molecular basis of resistance mechanisms and to develop control strategies.
Article
Biochemical Research Methods
Xian Hui, Jin-Meng Tian, Xin Wang, Zhen-Qian Zhang, Ya-Mei Zhao, Wen-Yun Gao, Heng Li
Summary: A new HPLC method was developed to comprehensively analyze the reactions catalyzed by AHAS/ALS. Compared to the classic Bauerle UV assay, the HPLC method provides a more precise analysis of enzyme characteristics and can measure the formation of products.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Wegi A. Wuddineh, Xia Xu, Gan-Yuan Zhong
Summary: This study identified VvALS1 as the best candidate gene for providing resistance to ALS-inhibiting herbicides in grapevine. The researchers found that chlorsulfuron and imazapyr herbicides showed a linear response curve in grapevine callus and in vitro shoots. By introducing amino acid substitutions in VvALS gene, the transgenic plants exhibited high resistance to chlorsulfuron or imazapyr herbicides. This research highlights the potential use of VvALS mutations as a selectable marker and enables gene-editing in grapevine improvement.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Shivani, Satvir Kaur Grewal, Ranjit Kaur Gill, Harpreet Kaur Virk, Rachana D. Bhardwaj
Summary: Yield reduction in lentil crop due to weed infestation is a major obstacle to its growth. This study investigated the impact of the selective herbicide imazethapyr (IM) on lentil genotypes. IM treatment led to a decrease in chlorophyll and carotenoid content, particularly in susceptible genotypes. Susceptible genotypes also showed reduced plant height, lower number of pods, and decreased seed yield under IM treatment. In contrast, tolerant genotypes had higher acetolactate synthase (ALS) activity, antioxidant capacity, and glycine betaine content, as well as less decrease in relative leaf water content, resulting in higher seed yield. These findings suggest that the identified IM tolerance mechanism can be used to enhance herbicide resistance in lentil.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2022)
Article
Agronomy
Tiancheng Lou, Ke Wang, Junmin Chen, Jingjing Cao, Tao Gu, Liben Jiang, Yuanlai Lou, Rongxiang Cao, Hongchun Wang
Summary: Tribenuron-methyl-resistant cleavers have been reported in the major winter wheat farming region in China. The study evaluated the frequency and distribution of multiple herbicide resistance in cleavers populations across Jiangsu Province from 2017 to 2020. The results highlight the importance of diversity in herbicide sites of action for effective weed management.
Article
Plant Sciences
Mikel Vicente Eceiza, Maria Barco-Antonanzas, Miriam Gil-Monreal, Michiel Huybrechts, Ana Zabalza, Ann Cuypers, Mercedes Royuela
Summary: The aim of this study was to investigate the role of oxidative stress in the mode of action of ALS inhibiting herbicides. Two populations of Amaranthus palmeri S. Watson from Spain (sensitive and resistant to nicosulfuron, due to mutated ALS) were subjected to nicosulfuron treatment at different rates. The results showed that oxidative stress is linked to ALS inhibition, but a supralethal nicosulfuron rate was needed to induce a significant oxidative stress response in the sensitive population, indicating that ALS inhibitors' lethality is not entirely dependent on oxidative stress.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Lucie Meyer, Fanny Pernin, Severine Michel, Geraldine Bailly, Bruno Chauvel, Valerie Le Corre, Christophe Delye
Summary: Assessing the capacity of weeds to develop herbicide resistance before it occurs in the field is crucial for effective chemical weed control. This study used herbicide selection and controlled crosses to accelerate the evolution of resistance to two commonly used herbicides in Ambrosia artemisiifolia. The results showed that non-target-site-based resistance (NTSR) to herbicides was significantly increased in just two generations of selection. NTSR to the tested herbicides emerged and was reinforced in subsequent generations. Further bioassays confirmed the emergence of resistance to imazamox and/or tribenuron in multiple A. artemisiifolia populations. NTSR was found to be the predominant type of resistance, with only one population showing a mutant ALS allele in addition to NTSR. This study highlights the importance of pre-emptive assessment of resistance evolution in weed populations.
Article
Agronomy
Barbara Wrzesinska, Tadeusz Praczyk
Summary: Centaurea cyanus, a weed species commonly found in fields with cereals, sugar beet, and maize, shows resistance mainly against acetolactate synthase (ALS) inhibitors. Genetic variability in ALS sequence has been observed among resistant plants, with four amino acid sequence changes identified in some resistant plants compared to susceptible ones. Further research is needed to confirm the significance of these mutations in herbicide resistance development.
Article
Biochemistry & Molecular Biology
Christy Grobbler, Bernardino Virdis, Amanda Nouwens, Falk Harnisch, Korneel Rabaey, Philip L. Bond
BIOELECTROCHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Daniel Dashevsky, Jordan Debono, Darin Rokyta, Amanda Nouwens, Peter Josh, Bryan G. Fry
JOURNAL OF MOLECULAR EVOLUTION
(2018)
Article
Multidisciplinary Sciences
Mario D. Garcia, Sheena M. H. Chua, Yu-Shang Low, Yu-Ting Lee, Kylie Agnew-Francis, Jian-Guo Wang, Amanda Nouwens, Thierry Lonhienne, Craig M. Williams, James A. Fraser, Luke W. Guddat
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Toxicology
Jordan Debono, Mettine H. A. Bos, Amanda Nouwens, Lilin Ge, Nathaniel Frank, Hang Fai Kwok, Bryan G. Fry
TOXICOLOGY IN VITRO
(2019)
Article
Environmental Sciences
Shu-Hong Gao, Jun Yuan Ho, Lu Fan, Amanda Nouwens, Robert D. Hoelzle, Benjamin Schulz, Jianhua Guo, Jizhong Zhou, Zhiguo Yuan, Philip L. Bond
SCIENCE OF THE TOTAL ENVIRONMENT
(2019)
Article
Biochemistry & Molecular Biology
Yaramah M. Zalucki, Freda E-C Jen, Cassandra L. Pegg, Amanda S. Nouwens, Benjamin L. Schulz, Michael P. Jennings
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Jordan Debono, Daniel Dashevsky, Amanda Nouwens, Bryan G. Fry
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Gry Brandt Boe-Hansen, Joao Paulo A. Rego, Nana Satake, Bronwyn Venus, Pawel Sadowski, Amanda Nouwens, Yutao Li, Michael McGowan
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2020)
Article
Food Science & Technology
Holger Scheib, K. Anne-Isola Nekaris, Johanna Rode-Margono, Lotten Ragnarsson, Kate Baumann, James S. Dobson, Wirdateti Wirdateti, Amanda Nouwens, Vincent Nijman, Paolo Martelli, Rui Ma, Richard J. Lewis, Hang Fai Kwok, Bryan Grieg Fry
Article
Multidisciplinary Sciences
Thierry Lonhienne, Yu Shang Low, Mario D. Garcia, Tristan Croll, Yan Gao, Quan Wang, Lou Brillault, Craig M. Williams, James A. Fraser, Ross P. McGeary, Nicholas P. West, Michael J. Landsberg, Zihe Rao, Gerhard Schenk, Luke W. Guddat
Article
Food Science & Technology
Daniel Dashevsky, Darin Rokyta, Nathaniel Frank, Amanda Nouwens, Bryan G. Fry
Summary: The genus Calliophis is the most basal branch of the family Elapidae, with some species developing highly elongated venom glands. Recent research has shown that C. bivirgatus has evolved a seemingly unique toxin that produces spastic paralysis in prey by acting on voltage-gated sodium channels. The broader clade of C. bivirgatus toxins contains a variety of toxins including classic elapid eight-cysteine three-finger toxins and delta-elapitoxins, with some similarities to cytotoxins from other elapids, but those characterized are not cytotoxic and show no clear relationships to toxins of known function from other species.
Article
Biochemistry & Molecular Biology
Tehseen Rubbab, Cassandra L. Pegg, Toan K. Phung, Amanda S. Nouwens, K. Y. Benjamin Yeo, Lucia F. Zacchi, Amna Muhammad, S. M. Saqlan Naqvi, Benjamin L. Schulz
Summary: The University of Queensland, School of Chemistry and Molecular Biosciences Mass Spectrometry Facility provided assistance and expertise. This work was supported by a grant from the HEC Pakistan to Tehseen Rubbab under the International Research Support Initiative Program.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Thierry Lonhienne, Yan Cheng, Mario D. Garcia, Shu Hong Hu, Yu Shang Low, Gerhard Schenk, Craig M. Williams, Luke W. Guddat
Summary: Acetohydroxyacid synthase (AHAS) is the target of more than 50 commercial herbicides, with many site-of-action resistance isolates identified in weeds. The mutations at P197 and W574 of AHAS can reduce the binding affinity of herbicides and abolish time-dependent accumulative inhibition. However, the bulky herbicide bispyribac is able to counteract the detrimental effects of these mutations, explaining the absence of site-of-action resistance to this herbicide.
NATURE COMMUNICATIONS
(2022)
Article
Polymer Science
Maria Eduarda Sevilla, Mario D. D. Garcia, Yunierkis Perez-Castillo, Vinicio Armijos-Jaramillo, Santiago Casado, Karla Vizuete, Alexis Debut, Liliana Cerda-Mejia
Summary: Due to the lack of efficient treatment of plastic waste, extensive plastic production has become a serious environmental and health problem. This study focuses on the enzymatic degradation of PET by IsPETase, a cutinase-like enzyme, to completely break down the polymer. Through rational design and mutation of the active site, the researchers were able to enhance the PET-degrading activity of IsPETase, particularly with the S238Y mutant. The modified enzyme showed a 3.3-fold increase in degradation activity compared to the wild-type enzyme and was capable of breaking down highly crystalline PET found in commercial soft drink bottles.
Article
Veterinary Sciences
Thomas P. Karbanowicz, Amanda Nouwens, Ala E. Tabor, Manuel Rodriguez-Valle
VETERINARY SCIENCES
(2018)