Journal
PLOS ONE
Volume 12, Issue 5, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0177182
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Funding
- Project of the Bio-oriented Technology Research Advancement Institution, NARO
- JSPS KAKENHI [25660243]
- Morinaga Houshikai Research Grant
- Grants-in-Aid for Scientific Research [17H03926, 16H05845, 17H03927, 25660243] Funding Source: KAKEN
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MicroRNA (miRNA) in tissue and liquid samples have been shown to be associated with many diseases including inflammation. We aimed to identify inflammation-related miRNA expression level in the bovine mastitis milk. Expression level of inflammation-related miRNA in milk from mastitis-affected and normal cows was analyzed using qPCR. We found that expression level of miR-21, miR-146a, miR-155, miR-222, and miR-383 was significantly upregulated in California mastitis test positive (CMT+) milk. We further analyzed these miRNA using a chip-based QuantStudio Digital PCR System. The digital PCR results correlated with those of qPCR, demonstrating upregulation of miR-21, miR-146a, miR-155, miR222, and miR-383 in CMT+milk. In conclusion, we identified miRNA that are upregulated in CMT+milk. These miRNA exhibited sensitivity and specificity greater than 80% for differentiating between CMT+milk and normal milk. Our findings suggest that inflammation-related miRNA expression level in the bovine milk was affected by mastitis, and miRNA in milk have potential for use as biomarkers of bovine mastitis.
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