Article
Biology
Frank K. Lee, Jane C. Lee, Bo Shui, Shaun Reining, Megan Jibilian, David M. Small, Jason S. Jones, Nathaniel H. Allan-Rahill, Michael Re Lamont, Megan A. Rizzo, Sendoa Tajada, Manuel F. Navedo, Luis Fernando Santana, Nozomi Nishimura, Michael Kotlikoff
Summary: Optogenetic effectors and sensors in mice offer a powerful tool to study cellular signaling and have immense potential for investigating physiological processes.
Article
Nanoscience & Nanotechnology
Haiyan Yang, Fujie Jiang, Xiaojuan Ji, Lu Wang, Yaotai Wang, Liang Zhang, Yu Tang, Disen Wang, Yong Luo, Ningshan Li, Qi Wang, Jianzhong Zou
Summary: The novel strategy of combining bacteriotherapy with HIFU therapy in this study showed significantly higher tumor inhibition rate up to 87% compared to monotherapies, providing a promising approach for more effective breast cancer treatment.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Leonid A. Ilchuk, Marina V. Kubekina, Yulia D. Okulova, Yulia Yu. Silaeva, Victor V. Tatarskiy, Maxim A. Filatov, Alexandra V. Bruter
Summary: The Mediator complex is a multi-subunit protein complex that regulates eukaryotic gene transcription by facilitating the interaction between transcriptional factors and RNA polymerase II. While most studies focus on simple models like tumor cell lines and yeast, transgenic mouse models are necessary to explore the role of Mediator components in physiological processes, disease, and development. Due to the embryonic lethality of constitutive knockout models, conditional knockout models and corresponding activator strains are required. Recent developments in genetic engineering techniques have made these models more accessible. This review discusses existing mouse models for studying the Mediator and the data obtained from corresponding experiments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Yukiko Ohara, Lisa Fujimura, Akemi Sakamoto, Youichi Teratake, Shuichi Hiraoka, Haruhiko Koseki, Takeshi Saito, Keita Terui, Tetsuya Mitsunaga, Mitsuyuki Nakata, Hideo Yoshida, Masahiko Hatano
Summary: The study found that the megacolon and enteric neuron hyperplasia in KO mice are influenced by genetic background. BALB/c KO mice may serve as a valuable model for functional gastrointestinal diseases such as chronic constipation, aiding in research on underlying mechanisms and providing a foundation for treatment development.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Ithai Rabinowitch, Bishal Upadhyaya, Aaradhya Pant, Dolev Galski, Lena Kreines, Jihong Bai
Summary: Loss of neurons can impair neural circuit function, but in this study, genetically engineered electrical synapses were used to reroute information flow in a damaged chemosensory circuit, restoring organism behavior. Additional electrical synapses were found to help restore circuit function by amplifying weakened neuronal signals and emulating the wild-type circuit.
Article
Biochemistry & Molecular Biology
Da-Som Kim, Wonjun Ji, Dong Ha Kim, Yun Jung Choi, Kyungtaek Im, Chae Won Lee, Jeongin Cho, Joongkee Min, Dong-Cheol Woo, Chang-Min Choi, Jae Cheol Lee, Young Hoon Sung, Jin Kyung Rho
Summary: A novel transgenic mouse model expressing human EGFR mutations was generated in this study, providing a valuable tool for studying drug resistance and evaluating the efficacy of new drugs. The expression of target genes and lung cancer occurrence were monitored using in vivo imaging systems, offering an effective approach for studying tumorigenesis and the development of targeted agents.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Hailing Yao, Huiying Shi, Chen Jiang, Mengke Fan, Yurui Zhang, Wei Qian, Rong Lin
Summary: The study revealed that L-Fucose could promote neurogenesis and gliogenesis derived from ENPCs by inhibiting the SMAD2 signaling, facilitating ENS regeneration and gastrointestinal motility recovery in type 1 diabetic mice.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Vu Thu Thuy Nguyen, Lena Bruecker, Ann-Kathrin Volz, Julia C. Baumgaertner, Malena dos Santos Guilherme, Francesco Valeri, Helen May-Simera, Kristina Endres
Summary: The study suggests a potential link between neurodegenerative diseases like Alzheimer's disease (AD) and abnormalities in gut function and alterations in primary cilia structure, warranting further investigation for confirmation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Yoon-Young Kim, Jin-Soo Kim, Jeong-Hwan Che, Seung-Yup Ku, Byeong-Cheol Kang, Jun-Won Yun
Summary: Immunodeficient animal models serve as valuable tools for stem cell research, with different gene defects resulting in a variety of phenotypes. Selecting the most appropriate immunodeficient strain, target gene, and animal species based on research type, mutant gene effects, and similarity to human immunological features is crucial for guiding researchers in stem cell research.
Article
Medicine, Research & Experimental
S. B. Panza, R. Vargas, S. L. Balbo, M. L. Bonfleur, D. C. T. Granzotto, D. M. G. Sant'Ana, G. A. Nogueira-Melo
Summary: This study indicates that perinatal exposure to glyphosate combined with a high-fat diet in adulthood increases the risk of jejunum inflammation and dysfunction. Specifically, exposure changes the morphology of the intestinal wall, increases certain cell types, and affects the enteric nervous system.
Article
Genetics & Heredity
Wendy Yang, Szu-Chieh Chen, Tse-En Wang, Pei-Shiue Tsai, Jeng-Chang Chen, Pei-Lung Chen
Summary: A study identified an L1CAM gene variation associated with mild hydrocephalus and HSCR, and introduced a similar variation into mice using the CRISPR-EZ method. Different phenotypes were observed in the mice, with no prominent hydrocephalus or HSCR-like symptoms, but male infertility was noticed after three generations. Shorter L1cam isoform proteins were found to be overexpressed in the intestine of L1cam knockdown mice, indicating their potential role in protecting against HSCR.
Article
Infectious Diseases
Giulia Bernabe, Mahmoud Elsayed Mosaad Shalata, Veronica Zatta, Massimo Bellato, Andrea Porzionato, Ignazio Castagliuolo, Paola Brun
Summary: The side effects of antibiotic treatment are directly related to intestinal dysbiosis. This study examined the long-term effects of antibiotic-induced dysbiosis on the enteric nervous system and the impact of spontaneous restoration of gut microbiota on gastrointestinal functions. Two weeks of antibiotic treatment significantly altered gut microbial composition, and some bacterial genera did not regain their abundance after six weeks of discontinuation.
Review
Biochemistry & Molecular Biology
Martina Montanari, Paola Imbriani, Paola Bonsi, Giuseppina Martella, Antonella Peppe
Summary: The enteric nervous system (ENS), composed of neurons and glial cells, regulates the motor and secretory functions of the gastrointestinal tract. Dysfunction of the connections between the brain and the GI tract is implicated in Parkinson's disease (PD). Alterations in the ENS, including α-synuclein aggregation, occur early in the disease and spread to the central nervous system. This review highlights the role of the ENS in PD pathogenesis and its potential as a target for new therapeutic approaches.
Article
Gastroenterology & Hepatology
Mengke Fan, Huiying Shi, Hailing Yao, Weijun Wang, Yurui Zhang, Chen Jiang, Rong Lin
Summary: Our previous study showed that transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) promotes functional enteric nerve regeneration in denervated mice but not through direct transdifferentiation. Homeostasis of the adult enteric nervous system (ENS) is maintained by enteric neural precursor cells (ENPCs). Whether ENPCs are a source of regenerated nerves in denervated mice remains unknown.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Cell Biology
Sukhada Bhave, Richard A. Guyer, Nicole Picard, Meredith Omer, Ryo Hotta, Allan M. Goldstein
Summary: Patients with Hirschsprung disease and Ednrb(-/-) mice lack a certain neuronal subpopulation, leading to gastrointestinal dysfunction that persists even after surgical correction.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)