Article
Chemistry, Medicinal
Jeongjin Park, Dakyung Kim, Minhee Lee, Sangshin Han, Woojin Jun, Hyun Mook Jung, Yean Kyoung Koo, Gwi Hwan Na, Sang Hun Han, Jehee Han, Ok-Kyung Kim
Summary: This study found that enzyme-treated caviar powder extract (CV) can protect keratinocytes against oxidative stress and inflammation induced by UVB irradiation by increasing antioxidant enzyme activities, elevating sphingomyelin and hyaluronic acid levels, and reducing inflammatory cytokine production.
Article
Biotechnology & Applied Microbiology
Shiva Izadi, Vinny Kunnummel, Herta Steinkellner, Stefan Werner, Alexandra Castilho
Summary: There is a demand to increase manufacturing capacities for recombinant protein-based drugs. Novel expression systems such as plants are being explored for faster and more flexible production. In this study, we discuss the technical issues and solutions for achieving efficient protein sialylation in plants. We also evaluate different strategies for delivering the sialylation pathway genes and demonstrate the scalability of protein sialylation for large-scale production.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Yana Sindarovska, Mykola Kuchuk
Summary: This study proposes a new system for producing recombinant proteins through transient expression technology in Nicotiana benthamiana plants grown in aseptic conditions. The system allows for easier and more efficient production of high levels of recombinant proteins.
Article
Biochemistry & Molecular Biology
Hui-Jie Jia, Yuchen Ge, Jing Xia, Yi-ling Shi, Xiao-bo Wang
Summary: Belinostat (PXD101), a histone deacetylase inhibitor, effectively inhibits cellular senescence and skin photoaging by inhibiting the UVB-induced signaling pathway.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Lukas Eidenberger, Florian Eminger, Alexandra Castilho, Herta Steinkellner
Summary: This study investigated the capability of three commonly used transient expression vectors to express a multi-component protein with controlled and modified N-glycosylation. The results showed that all three expression vectors produced similar quantities of targeted N-glycosylation, demonstrating the universal application of plant-based glycoengineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Nicolo Alvisi, Kim van Noort, Sarlita Dwiani, Nathan Geschiere, Octavina Sukarta, Koen Varossieau, Dieu-Linh Nguyen, Richard Strasser, Cornelis H. Hokke, Arjen Schots, Ruud H. P. Wilbers
Summary: Recent research has shown that glycoproteins from parasitic worms can be produced transiently in Nicotiana benthamiana plants by co-expressing desired glycosyltransferases. However, efficient modification of N-glycans in plants seems to be hindered by specific enzymes.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Lilya Kopertekh, Sven Reichardt
Summary: Transient expression in Nicotiana benthamiana was enhanced by transforming the At-CycD2 gene, resulting in increased leaf and stem biomass fractions. The transgenic line At-CycD2-15 showed accelerated above-ground biomass accumulation and enlarged leaf area, attributed to an increase in epidermal cell number compensated by slightly reduced cell size. The production capacity of At-CycD2-15 line for transiently expressed proteins was significantly higher compared to the wild type, demonstrating the potential of the cell cycle regulator gene At-CycD2 in enhancing recombinant protein yield.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Akira Yata, Shohei Nosaki, Akiyoshi Yoda, Takahito Nomura, Kenji Miura
Summary: In this study, we demonstrated the successful production of a large amount of 4-deoxyorobanchol (4DO) in tobacco leaves using a transient expression system. We also found that the stability of 4DO can be maintained in dried leaves for an extended period of time.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Cheng Qin, Mohammad Abass Ahanger, Bo Lin, Ziguang Huang, Jie Zhou, Nadeem Ahmed, Suilong Ai, Nabil Sa Mustafa, Muhammad Ashraf, Lixin Zhang
Summary: The study showed that ACh treatment promoted differential gene expression in Nicotiana benthamiana under salt stress, decreased antioxidant enzyme activity, and increased cell wall biosynthesis and hormone signaling pathway gene expression.
Review
Plant Sciences
Manuel Rodriguez-Concepcion, Jose-Antonio Daros
Summary: Enriching food with health-promoting compounds like carotenoids is an effective approach to combat unhealthy eating habits. Carotenoids are precursors of vitamin A and can reduce the risk of various chronic diseases. Recent advances in transient expression systems have provided valuable insights into the synthesis and storage of carotenoids in plants, enabling efficient biofortification of plant tissues.
CURRENT OPINION IN PLANT BIOLOGY
(2022)
Article
Plant Sciences
Advaita Acarya Singh, Priyen Pillay, Previn Naicker, Kabamba Alexandre, Kanyane Malatji, Lukas Mach, Herta Steinkellner, Juan Vorster, Rachel Chikwamba, Tsepo L. L. Tsekoa
Summary: This study demonstrates the role of cysteine proteases in the proteolytic degradation of an antibody produced in tobacco plants and shows that transient coexpression of CRISPR/Cas9 vectors targeting cysteine protease genes can decrease antibody degradation. The findings suggest that this plant-based strategy can improve antibody quality without affecting its functional activity.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Tanapati Phakham, Christine Joy Bulaon, Narach Khorattanakulchai, Balamurugan Shanmugaraj, Supranee Buranapraditkun, Chatikorn Boonkrai, Sarintip Sooksai, Nattiya Hirankarn, Yoshito Abe, Richard Strasser, Kaewta Rattanapisit, Waranyoo Phoolcharoen
Summary: This study demonstrates that the plant-based system in Nicotiana benthamiana can efficiently and rapidly produce Pembrolizumab antibody with expected molecular weight, structural characteristics, and functional properties, comparable to the commercial product Keytruda(R).
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Areum Yun, Joohyun Kang, Juhun Lee, Shi-Jian Song, Inhwan Hwang
Summary: Plants are advantageous for producing recombinant proteins due to their low cost and scalability. However, current plant protein expression systems need improvement in achieving high yields. In this study, a robust expression system was developed using a hybrid promoter, FM ' M-UD, and an artificial terminator, 3PRt, which resulted in significant increases in protein levels compared to traditional promoters. Furthermore, the combination of FM ' M-UD/3PRt with an artificial transcription factor further enhanced protein production. Overall, this novel expression system allows for the efficient production of large quantities of recombinant proteins in plants.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Lilya Kopertekh, Sven Reichardt
Summary: In this study, the introduction of the At-CDC27a gene from Arabidopsis thaliana into Nicotiana benthamiana resulted in increased plant biomass production and protein accumulation, potentially improving the yield of recombinant proteins in closed facilities.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Dermatology
Ji Yeon Hong, Hye Sung Han, Ji Hyun Youn, Hyun-wook Kim, Hyun-Seung Ryu, Kui Young Park
Summary: The study shows that 590nm yellow light LED irradiation can be effectively and safely used for anti-oxidative and anti-inflammatory treatment of photoaged skin, reducing UVB-induced oxidative stress and inflammation, promoting collagen recovery, suppressing MMP-9 levels, and decreasing phosphorylation of NF-kappa B and COX-2.
EXPERIMENTAL DERMATOLOGY
(2022)
