Article
Biology
Neda Javadian, Ghasem Karimzadeh, Mohsen Sharifi, Ahmad Moieni
Summary: In this study, tetraploid callus cultures of Linum album, a plant endemic to Iran known for its medicinal properties, were established to increase the production of podophyllotoxin (PTOX). The tetraploid calli produced 2.1-fold more PTOX compared to diploid calli. The up-regulation of key genes involved in PTOX biosynthesis was observed in the tetraploid calli, indicating that autopolyploidy could be an effective strategy for enhancing PTOX production in L. album callus.
Article
Agronomy
Vijayakumari Narukulla, Yogesh Lahane, Krutika Fiske, Shashi Pandey, Vasileios Ziogas
Summary: In this study, stable tetraploids were developed in citrus rootstock cultivars including Rough lemon, Rangpur lime, and Alemow through in vitro colchicine treatments. The treatment with 0.1% colchicine for 24 hours resulted in the highest tetraploid induction percentage (18.3%) in all the rootstock cultivars.
Article
Biochemical Research Methods
Keondo Lee, Seong-Eun Kim, Junsang Doh, Keehoon Kim, Wan Kyun Chung
Summary: The study introduces a user-friendly image-activated microfluidic sorting technique based on a fast deep learning model, enabling sorting decisions within 3 ms. The sorter has a latency of 2.5 ms and demonstrated high sorting purities and throughput.
Article
Biochemical Research Methods
Tobias Gerling, Neus Godino, Felix Pfisterer, Nina Hupf, Michael Kirschbaum
Summary: Continuous flow cell sorting based on image analysis is a powerful concept that isolates specialized cell types through spatially-resolved features in cells. A new low-complexity microfluidic approach is presented, which provides high-resolution images and allows thorough image analysis. Live T cells were successfully sorted based on subcellular localization of fluorescence signals with high purities and recovery rates.
Article
Immunology
Francesco Vallania, Liron Zisman, Claudia Macaubas, Shu-Chen Hung, Narendiran Rajasekaran, Sonia Mason, Jonathan Graf, Mary Nakamura, Elizabeth D. Mellins, Purvesh Khatri
Summary: Monocytes in humans have three distinct subsets, each with different functions, but analyzing the contribution of these subsets to disease is hindered by limited samples and cohorts; publicly available transcriptome profiles offer a powerful alternative source of data for discovering gene expression characteristics of monocyte subsets.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Antonio Durate Pagano, Natieli Machado Goncalves, William Borges Domingues, Tony Leandro Rezende da Silveira, Mateus Tavares Kutter, Antonio Sergio Varela Jr, Carine Dahl Corcini, Mariana Cavalcanti Nascimento, Luana Ferreira Viana dos Reis, Patricia Gomes Costa, Adalto Bianchini, Matheus Vieira Volcan, Mariana Harter Remia, Vinicius Farias Campos
Summary: This study analyzed the toxic effects of Roundup Transorb (R) on the endangered Neotropical annual killifish Austrolebias charrua by evaluating molecular and biochemical biomarkers. The findings showed that exposure to Roundup significantly increased the production of reactive oxygen species, lipid peroxidation, and DNA damage in the fish's blood cells. It also decreased membrane fluidity. Gene expression analysis revealed alterations in genes associated with oxidative stress and antioxidant defense. This study provides new insights into adaptive mechanisms of A. charrua in response to Roundup, and it has important implications for environmental monitoring and aquatic toxicology assessment.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Agriculture, Multidisciplinary
H. Razmi, R. Amiri Fahliani, B. Kavoosi, A. Masoumiasl
Summary: This study induced polyploidy in strawberries using colchicine and found that a concentration of 0.7% was the most effective. The tetraploid plants showed larger fruit size and lower stomata number compared to the diploid plants.
JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY
(2022)
Article
Agricultural Engineering
Di Liu, Miaoyin Dong, Mengfei Li, Ling Jin, Jianhe Wei, Paul W. Pare
Summary: Sinopodophyllum hexandrum is a perennial herb with medicinal properties. Its fruit, also called Himalayan Mayapple, undergoes significant metabolic changes during ripening, with a decrease in podophyllotoxin content and an increase in flavonoids and polysaccharides. Analysis of fruit extracts and gene expression levels revealed the involvement of phenylpropanoid metabolism and glycometabolism in the ripening process.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Pharmacology & Pharmacy
Angela-Patricia Hernandez, Paula Diez, Pablo A. Garcia, Martin Perez-Andres, Anzhela Veselinova, Pablo G. Jambrina, Arturo San Feliciano, David Diez, Manuel Fuentes, Ma Angeles Castro
Summary: New lignohydroquinone conjugates (L-HQs) were synthesized using the hybridization strategy and showed dual cytotoxic effect against colorectal cancer cells. These conjugates were obtained from natural product podophyllotoxin and semisynthetic terpenylnaphthohydroquinones. The results demonstrate the validity of the hybridization strategy and suggest further research on non-lactonic cyclolignans.
Article
Multidisciplinary Sciences
Zahra Danaeipour, Ghasemali Garoosi, Masoud Tohidfar, Mohammad Reza Bakhtiarizadeh, Mohammad Hossein Mirjalili
Summary: Linum album is a valuable source of anticancer compounds called lignans, which play a crucial role in the plant's defense system. Analyzing the plant's response to various stresses helps to understand the significance of lignans in plant defense.
SCIENTIFIC REPORTS
(2023)
Article
Plant Sciences
Fan Qi, Fu Wang, Chunxiao Xiaoyang, Zhenhui Wang, Yujie Lin, Zhanwu Peng, Jun Zhang, Ningning Wang, Jian Zhang
Summary: This study conducted transcriptome analysis to analyze the gene expression profiles of different organs in flax. The results identified differentially expressed genes and organ-specific expression patterns. Gene ontology enrichment analysis revealed the biological processes and molecular functions associated with organ growth. The study also investigated the role of AP2 transcription factors in regulating flax organ development and stress response. Overall, this research provides valuable insights into the molecular mechanisms of flax organ development and has implications for genetic improvement of flax crops.
Article
Biochemistry & Molecular Biology
Jill A. Jenkins, Katherine R. Hartop, Ghadeer Bukhari, Debra E. Howton, Kelly L. Smalling, Scott V. Mize, Michelle L. Hladik, Darren Johnson, Rassa O. Draugelis-Dale, Bonnie L. Brown
Summary: Neonicotinoids, specifically thiamethoxam and clothianidin, were applied on juvenile African clawed frogs to study their impact on growth, development, liver enzyme activity, and gene expression. Results showed that high concentrations of THX led to higher mortality rates, while low concentrations impaired development and weight gain. Liver reductase activity was highest in the control group, with the greatest cytotoxicity observed in the high THX group. Brain tissues showed more transcriptional activity compared to liver tissues, highlighting the importance of considering multiple complexities in ecotoxicological studies with non-target amphibians.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Nina Kresic, Marina Prislin, Dunja Vlahovic, Petar Kostesic, Ivana Ljolje, Dragan Brnic, Nenad Turk, Andrija Musulin, Boris Habrun
Summary: During cultivation, canine adipose-derived mesenchymal stem cells showed variations in the expression of surface markers, with an increase in CD90, CD44, and CD29, a decrease in CD271 and CD73, and a decrease in initially high levels of CD105. CD45 and CD14 were not expressed. Interestingly, there was a significant decrease in CD73 expression from early to late passages, despite stable gene expression. Positive marker expression was highest up to passage 4, suggesting the impact of culture time on therapeutic properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Clinical Neurology
Ancor Sanz-Garcia, Patricia Sanchez-Jimenez, Inmaculada Granero-Cremades, Maria de Toledo, Paloma Pulido, Marta Navas, Jose Maria Frade, Matilde Desiree Pereboom-Maicas, Cristina Virginia Torres-Diaz, Maria C. Ovejero-Benito
Summary: This study examined the presence of tetraploid neurons and astrocytes in drug-resistant epilepsy patients. Both cell types were found in healthy individuals as well as in patients with drug-resistant epilepsy. The hippocampus of drug-resistant epilepsy patients had a higher number of tetraploid neurons and astrocytes compared to controls.
NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY
(2023)
Article
Agricultural Engineering
Qiaozhu Zhao, Meiling Li, Mengfei Li, Ling Jin, Jianhe Wei
Summary: The study revealed that growth parameters of Sinopodophyllum hexandrum significantly increased with increasing plant age, showing three distinct morphological variants. The accumulation of lignans, flavonoids, and phenolic compounds, as well as in vitro antioxidant capacity, reached maximum levels at 5-6 years. The expression levels of key genes involved in lignan and flavonoid biosynthesis were up-regulated with increasing plant age. These findings provide valuable insights for sustainable cultivation and collection of S. hexandrum.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Chemistry, Applied
Samareh Babaei, Vahid Niknam, Mehrdad Behmanesh
Summary: Research has shown that Nitric Oxide (NO) and salt can increase the content of crocetin in Crocus sativus, affecting the biosynthesis of apocarotenoids by influencing gene expression.
NATURAL PRODUCT RESEARCH
(2021)
Article
Plant Sciences
Samareh Babaei, Vahid Niknam, Mehrdad Behmanesh
Summary: The study showed that treating Crocus sativus with nitric oxide improved plant growth under salinity stress by promoting compatible solutes accumulation, inducing antioxidative enzyme activities, and increasing the biosynthesis of secondary metabolites. Conversely, salicylic acid treatment did not have a positive effect on plant growth under salinity stress.
Article
Medicine, Research & Experimental
Mojgan Gharipour, Mehrdad Behmanesh, Mansoor Salehi, Golnaz Vaseghi, Pouya Nezafati, Minoo Dianatkhah, Elham Khosravi, Mohsen Hosseini, Masoumeh Sadeghi
ARCHIVES OF MEDICAL RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Zahra Bayat, Zahra Ghaemi, Mehrdad Behmanesh, Bahram M. Soltani
Summary: The TGF beta signaling pathway is involved in colorectal cancer progression, with miR-186-5p playing a crucial role by regulating the expression of SMAD6/7. Overexpression of miR-186-5p in CRC cells resulted in reduced proliferation, migration, and increased apoptosis, making it a potential novel tumor suppressive biomarker and therapeutic target.
BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Mohammad Farhad Vahidi, Javad Gharechahi, Mehrdad Behmanesh, Xue-Zhi Ding, Jian-Lin Han, Ghasem Hosseini Salekdeh
Summary: The rumen microbiota plays a crucial role in degrading ingested plant materials. This study examined how the physicochemical properties of forages influence the colonization and digestion by rumen microbiota. The results indicate that different forages have varying degradation rates in the rumen, with Treponema and Butyrivibrio playing key roles in microbial diversity.
Correction
Horticulture
Mehdi Babaei, Majid Shokrpour, Mohsen Sharifi, Seyed Alireza Salami
SCIENTIA HORTICULTURAE
(2021)
Article
Genetics & Heredity
Roya Amirinejad, Zeinab Shirvani-Farsani, Bahar Naghavi Gargari, Mohhamad Ali Sahraian, Bahram Mohammad Soltani, Mehrdad Behmanesh
Summary: This study revealed that 2-month VD treatment significantly altered the expression of MYH, OGG1, MTH1, and NRF2 genes in MS patients. There was a significant correlation between DNA repair genes and NRF2 expression, indicating the involvement of DNA repair genes in the physiopathology of MS patients.
Article
Environmental Sciences
Hamed Dadkhah-Aghdash, Mehdi Heydari, Hassan Zare-Maivan, Mohsen Sharifi, Isabel Miralles, Manuel Esteban Lucas-Borja
Summary: The study focused on the physiological and morphological responses of Brant's oak to pollution from gas refineries in the semiarid forests of Iran. Oak trees showed different responses in leaf traits depending on season and distance from the refinery, with rapid reaction responses and oxidative stress indicators observed in summer and autumn at closer distances to the pollution source. These traits could be used for efficient environmental monitoring of pollution in semiarid ecosystems.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Medicine, Research & Experimental
Elahe Sadeghi Sahebzad, Najmeh Tehranian, Anoshirvan Kazemnejad, Mohsen Sharifi, Faraz Mojab, Ali Azin
Summary: This study found that consumption of turmeric in stressed mice can increase serum adiponectin levels, improving sexual function and estradiol hormone profiling.
Article
Biotechnology & Applied Microbiology
Sedigheh Ahmadi-Sakha, Mohsen Sharifi, Vahid Niknam
Summary: This study investigated variations in amino acids and carbohydrates levels in Scrophularia striata at different growth phases in shake flask and bioreactor cultures using HPLC. The findings suggest that phenylalanine, tyrosine, rhamnose, and glucose are key precursors for the biosynthesis of phenylethanoid glycosides. Higher sucrose levels in bioreactor cultures indicate increased energy demands and metabolism rates of amino acids for glycolysis and tricarboxylic cycles.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Ecology
Hamed Dadkhah Aghdash, Hassan Zare-Maivan, Mehdi Heydari, Mohsen Sharifi, Manuel Esteban Lucas-Borja, Daniel C. Dey
Summary: The study revealed that toxic pollutants emitted from a gas refinery significantly affected seed germination and morphological characteristics of seedlings from maternal trees, with the best growth observed in seedlings from acorns collected at a distance of 10,000 m. The farthest distances from the refinery showed the highest values in seed germination and seedling growth. Seed germination traits were found to be most sensitive to gaseous toxic pollutants emitted from this source.
ECOLOGICAL ENGINEERING
(2021)
Article
Neurosciences
Fatemeh Khani-Habibabadi, Leila Zare, Mohammad Ali Sahraian, Mohammad Javan, Mehrdad Behmanesh
Summary: The study investigated the role of long non-coding RNAs in regulating BDNF gene expression and OPC cell maturation, demonstrating significant correlations between Malat1 and Hotair with BDNF in glial cells. Fingolimod treatment was found to enhance OPC myelination and Malat1 may play a role in the last stages of intrinsic OL myelination, regardless of treatment.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zahra Abedi Kichi, Lucia Natarelli, Saeed Sadeghian, Mohammad Ali Boroumand, Mehrdad Behmanesh, Christian Weber
Summary: Research found that orphan GPCR26 is downregulated in PBMC isolated from T2D patients, while GPR26 is initially upregulated in response to high glucose but decreased under chronic hyperglycemic conditions. GPR26 inversely correlated with BMI and HbA1c in diabetic patients. Knockdown of GPR26 enhanced monocyte inflammatory response and apoptosis.
Article
Plant Sciences
Nikoo Azad, Hassan Ebrahimzadeh, Mehrdad Behmanesh, Vahid Niknam
Summary: Given the health risks and environmental damages caused by synthetic herbicides and fertilizers, it is important to understand how plants respond to naturally derived bioactive substances. This study investigates the effects of certain benzoic acid derivatives on spearmint growth and the role of nitric oxide in alleviating growth inhibition. The results show that some phenolic acids inhibit growth while others stimulate growth and increase the accumulation of secondary metabolites.
JOURNAL OF PLANT NUTRITION
(2023)
Article
Agronomy
Farinaz Ziaee, Mohammadreza Hajjari, Seyed Reza Kazeminezhad, Mehrdad Behmanesh
Summary: This study revealed the potential role of SNHG7 in colorectal cancer, suggesting its involvement in cancer progression by modulating the availability of miRNAs. By analyzing different databases and conducting in silico studies, potential targets of SNHG7 were identified. This indicates that SNHG7 may serve as a promising biomarker for diagnosis and treatment of colorectal cancer.
