Article
Biotechnology & Applied Microbiology
Sakshi Bharti, Sebastian Ploch, Marco Thines
Summary: The downy mildew disease of sunflower caused by Plasmopara halstedii has a significant economic impact on sunflower cultivation. This study used whole transcriptome sequencing to investigate the developmental phases of Pl. halstedii infecting Helianthus annuus. By analyzing the upstream regulatory gene sequences of co-expressed genes, conserved motifs potentially involved in binding transcription factors were identified. These motifs were also associated with virulence related genes and could be further studied to understand their role in regulating pathogenesis stages.
Article
Agronomy
Maria Iwebor, Tatiana Antonova, Nina Araslanova, Svetlana Saukova, Yulia Pitinova, Ksenia Eliseeva, Aleksey Belorutskiy
Summary: Sunflower downy mildew caused by Plasmopara halstedii is a destructive and widespread disease. A new race 337 of the pathogen was identified for the first time in Russia in 2020, infecting various sunflower hybrids.
PLANT PROTECTION SCIENCE
(2022)
Article
Agronomy
Pedro Miranda-Fuentes, Ana B. Garcia-Carneros, Leire Molinero-Ruiz
Summary: The study identified diverse strains of P. halstedii in Spain, with an increasing proportion of highly virulent strains. Results suggest that the effectiveness of entomopathogenic fungi in controlling downy mildew is influenced by soil as a growth substrate and long infection times.
Article
Biotechnology & Applied Microbiology
Nuray Ozer, Arzu Coskuntuna, Temine Sabudak
Summary: The Trichoderma harzianum isolate TRIC8 treatment significantly increased the hypocotyl length of sunflower seedlings, reduced the sporulation density of the pathogen, and increased the accumulation of specific metabolites in the roots when challenged with Plasmopara halstedii. These metabolites may play a role in enhancing hypocotyl length and protecting sunflowers against downy mildew disease.
BIOCONTROL SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Nisha Nisha, Sergey Vinogradov, Katalin Korosi, Arbnora Berisha, Rita Ban
Summary: The study aimed to assess the sensitivity of Plasmopara halstedii isolates to mefenoxam, a previously effective active ingredient, through host responses to infection. Results showed clear differences in host responses and suggested that examining tissue reactions may be more accurate in assessing the sensitivity of P. halstedii isolates to mefenoxam.
Article
Microbiology
Rita Ban, Attila Kovacs, Nisha Nisha, Zoltan Palinkas, Mihaly Zalai, Ahmed Ibrahim Alrashid Yousif, Katalin Korosi
Summary: This study investigated the pathotype structure of Plasmopara halstedii in Hungary and six other European countries, identifying 18 new pathotypes, with two from the Asian part of Turkey. The detailed distribution of pathotypes in Hungary was also discussed in the study.
Article
Plant Sciences
Zahirul I. Talukder, William Underwood, Christopher G. Misar, Gerald J. Seiler, Xiwen Cai, Xuehui Li, Lili Qi
Summary: This study investigated the resistance against basal stalk rot (BSR) caused by Sclerotinia sclerotiorum in cultivated sunflower, introgressed from the wild sunflower Helianthus praecox ssp. runyonii. The research identified 19 BSR resistance quantitative trait loci (QTL) on nine sunflower chromosomes, with 16 of them deriving resistant alleles from H. praecox parent. These findings provide genetic markers for marker-assisted breeding to combat BSR in sunflower.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Gregory L. Owens, Marco Todesco, Natalia Bercovich, Jean-Sebastien Legare, Nora Mitchell, Kenneth D. Whitney, Loren H. Rieseberg
Summary: The origins of the texanus subspecies of Helianthus annuus in Texas are likely not from introgression with the local congener H. debilis, but rather from introgression with the sister species H. argophyllus. Genomic differentiation in H. a. texanus is mainly driven by large segregating inversions, some of which show signs of natural selection based on haplotype frequencies.
Article
Horticulture
Qixiu Huang, Zhonghua Lei, Lijun Xiang, Wangfeng Zhang, Li Zhang, Yan Gao
Summary: In this study, the transcriptomic changes of sunflower seedlings resistant and sensitive to Orobanche cumana infection were investigated. The resistance process was similar in both types of seedlings, but higher regulatory activity was observed in the resistant plants. The upregulated genes in the resistant plants were mainly involved in antioxidant responses, stress responses, stimulation responses, and metabolic processes. Three significantly upregulated genes in the resistant plants may be the main causes of O. cumana immunity. The study contributes to understanding the resistance mechanism in sunflower and breeding for O. cumana resistance.
Article
Plant Sciences
Zahirul I. Talukder, William Underwood, Christopher G. Misar, Xuehui Li, Gerald J. Seiler, Xiwen Cai, Lili Qi
Summary: This study identified loci governing basal stalk rot (BSR) resistance derived from H. petiolaris through an advanced backcross population (AB-QTL). A total of 14 QTL associated with BSR resistance were identified on nine chromosomes, with most alleles conferring increased BSR resistance coming from the H. petiolaris parent. The results suggest the potential for breeding sunflower cultivars with improved resistance to the BSR pathogen.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
L. L. Qi, X. W. Cai
Summary: Sunflower downy mildew is a serious disease that significantly reduces sunflower yields worldwide. Understanding the spread and evolution of the pathogen population is crucial for developing resistant sunflower lines. In this study, the presence of the resistance gene Pl(36) in the sunflower line 803-1 was confirmed and its genetic mapping was conducted.
MOLECULAR BREEDING
(2022)
Article
Plant Sciences
Faith A. Bankole, Baffour Badu-Apraku, Abiodun O. Salami, Titilayo D. O. Falade, Ranajit Bandyopadhyay, Alejandro Ortega-Beltran
Summary: The study found morphological and genetic variation among Exserohilum turcicum isolates in maize fields in Nigeria. This suggests that breeding for resistance to Northern Corn Leaf Blight (NCLB) in Nigeria requires selection for quantitative resistance.
Article
Plant Sciences
Md Shamimuzzaman, Guojia Ma, William Underwood, Lili Qi
Summary: In this study, we successfully cloned the R-11 gene conferring rust resistance in sunflower and found that the encoded protein shares structural and functional similarities with genes conferring plant resistance to biotrophic pathogens. Additionally, we identified differences in gene expression patterns associated with R-11-mediated resistance and uncovered the potential roles of terpenoid biosynthesis and metabolism in sunflower rust resistance.
Article
Agronomy
Katalin Korosi, Attila Kovacs, Nisha Nisha, Istvan Bota, Mihaly Perczel, Ahmed Ibrahim Alrashid Yousif, Jozsef Kiss, Rita Ban
Summary: The study in Hungary revealed that sunflower downy mildew is mainly caused by pathotype 704, but other pathotypes were also detected. Seven out of ten isolates showed resistance to mefenoxam, indicating high genetic variability in the pathogen and potential for fungicide resistance.
PLANT PROTECTION SCIENCE
(2021)
Article
Plant Sciences
Dana Sisou, Yaakov Tadmor, Dina Plakhine, Hammam Ziadna, Sariel Hubner, Hanan Eizenberg
Summary: This study identified a pre-haustorial resistance mechanism in a confectionery sunflower cultivar with robust and long-lasting resistance to sunflower broomrape, involving genes like beta-1,3-endoglucanase, beta-glucanase, and ethylene-responsive transcription factor 4 (ERF4) which were differentially expressed upon broomrape infestation. Transcriptomic investigation and histological examinations confirmed the formation of a physical barrier preventing the establishment of the broomrape into the sunflower roots.
Article
Biochemistry & Molecular Biology
Katerina Pernickova, Veronika Kolackova, Adam J. Lukaszewski, Chaolan Fan, Jan Vrana, Martin Duchoslav, Glyn Jenkins, Dylan Phillips, Olga Samajova, Michaela Sedlarova, Jozef Samaj, Jaroslav Dolezel, David Kopecky
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Veronika Kolackova, Katerina Pernickova, Jan Vrana, Martin Duchoslav, Glyn Jenkins, Dylan Phillips, Edina Turkosi, Olga Samajova, Michaela Sedlarova, Jozef Samaj, Jaroslav Dolezel, David Kopecky
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Agronomy
Barbora Mieslerova, Miloslav Kitner, Veronika Petrekova, Jitka Dvorakova, Michaela Sedlarova, Roger T. A. Cook, Ales Lebeda
PLANT PROTECTION SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Aditya Kumar, Ankush Prasad, Michaela Sedlarova, Brigitte Ksas, Michel Havaux, Pavel Pospisil
FREE RADICAL BIOLOGY AND MEDICINE
(2020)
Article
Forestry
Barbora Mieslerova, Michaela Sedlarova, Marketa Michutova, Veronika Petrekova, Roger Cook, Ales Lebeda
Article
Multidisciplinary Sciences
Aditya Kumar, Ankush Prasad, Michaele Sedlarova, Ravindra Kale, Laurie K. Frankel, Larry Sallans, Terry M. Bricker, Pavel Pospisil
Summary: This study investigates the oxidative modifications of D1 and D2 proteins in PSII by superoxide anion and hydroxyl radicals, revealing that the presence of alpha-tocopherol helps prevent oxidative damage to PSII.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Zuzana Sochorova, Neven Matocec, Ivana Kusan, Lukas Janosik, Jan Eckstein, Marcel Vega, Armin Mesic, Michaela Sedlarova, Ruben Martinez-Gil, Michal Sochor
Article
Biochemistry & Molecular Biology
Ankush Prasad, Renuka Ramalingam Manoharan, Michaela Sedlarova, Pavel Pospisil
Summary: The study utilizes U-937 and HL-60 cell lines as a model system to investigate the production of reactive oxygen species (ROS) through stimulation with PMA. Various techniques were used to confirm ROS formation and protein oxidation, providing valuable insights for expanding knowledge of differentiation and protein expression dynamics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Vladimir Skalicky, Tereza Vojtkova, Ales Pencik, Jan Vrana, Katarzyna Juzon, Veronika Kolackova, Michaela Sedlarova, Martin F. Kubes, Ondrej Novak
Summary: This study tested various nuclear isolation protocols and combined with mass spectrometry analysis to obtain information on the auxinome from isolated nuclei of Arabidopsis and tobacco cell cultures, revealing dynamic changes in auxin homeostasis within the nucleus. The methodological procedure combining flow cytometry and mass spectrometry offers new horizons for the study of auxin homeostasis at the subcellular level.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Zuzana Sochorova, Matteo Carbone, Michaela Sedlarova, Adam Polhorsky, Michal Sochor
Summary: This study describes Pseudoplectania africana as a new species and investigates the presence of crystals as a diagnostic character. The researchers found that Pseudoplectania africana has distinct morphological characteristics and forms a distinct lineage compared to other Pseudoplectania species.
Article
Biochemistry & Molecular Biology
Ankush Prasad, Claudio Rossi, Renuka Ramalingam Manoharan, Michaela Sedlarova, Lorenzo Cangeloni, Deepak Rathi, Gabriella Tamasi, Pavel Pospisil, Marco Consumi
Summary: In this study, the effect of four selected bioactive compounds on reducing oxidative stress in differentiating cells was investigated using U-937 cells as a model system. The results showed that these compounds can reduce the formation of MDA adducts, indicating their beneficial effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Miloslav Kitner, Marco Thines, Michaela Sedlarova, Lucie Vaculna, Rita Ban, Katalin Korosi, Maria Iwebor, Tatiana Antonova, Tahir Ali, Petr Nadvornik, Ales Lebeda, Otmar Spring
Summary: This study genetically analyzed P. halstedii samples collected across Europe and discovered two distinct lineages infecting sunflowers, which might represent different species. The evolution of virulence phenotypes was found to be independent of host-specific characteristics.
Article
Biochemistry & Molecular Biology
Renuka Ramalingam Manoharan, Michaela Sedlarova, Pavel Pospisil, Ankush Prasad
Summary: Reactive oxygen species play a crucial role in maintaining cellular homeostasis and redox signaling, but excessive production can lead to protein dysfunction and damage. This study focuses on understanding the process of radical-mediated protein oxidation in macrophages using the immuno-spin trapping technique. The results show the formation of hydroxyl radicals and lipid peroxidation products, indicating the need for further investigation into the specific proteins involved in macrophage activation and inflammation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2023)
Article
Biochemistry & Molecular Biology
Zuzana Kucerova, Marek Rac, Jaromir Mikulik, Ondrej Plihal, Pavel Pospisil, Magdalena Bryksova, Michaela Sedlarova, Karel Dolezal, Martina Spundova
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)