Article
Plant Sciences
Linling Zheng, Qi Wan, Honggang Wang, Changlin Guo, Xiaolei Niu, Xiaofei Zhang, Rui Zhang, Yinhua Chen, Kai Luo
Summary: This study provides a comprehensive analysis of the TIFY family genes in cassava and their roles in plant stress responses. The characterization of these genes and their phylogenetic relationships, as well as the prediction of protein interaction networks, offer valuable insights into their evolution and functions. The validation of gene expression patterns in response to different stress conditions further adds to our understanding of the TIFY family genes in cassava.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Applied
Haoqiang Yang, Na Yi, Shuai Zhao, Zhouyang Xiang, Mirza Faisal Qaseem, Biao Zheng, Huiling Li, Jia-Xun Feng, Ai-Min Wu
Summary: This research focused on the structure and composition of hemicellulose in the stems of cassava plants, revealing variations in hemicellulose content and molecular weight along the stem. These findings are valuable for better utilization of cassava plant waste and understanding structural changes of cassava hemicellulose during xylogenesis.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemistry & Molecular Biology
Anh Thu Vu, Yoshinori Utsumi, Chikako Utsumi, Maho Tanaka, Satoshi Takahashi, Daisuke Todaka, Yuri Kanno, Mitsunori Seo, Eigo Ando, Kaori Sako, Khurram Bashir, Toshinori Kinoshita, Xuan Hoi Pham, Motoaki Seki
Summary: This study reveals that ethanol priming enhances drought tolerance in cassava by regulating stomatal closure. Ethanol pretreatment induces abscisic acid (ABA) accumulation and stomatal closure, resulting in reduced transpiration rate and increased water content and starch accumulation in leaves during drought stress. The transcriptomic analysis shows that ethanol pretreatment upregulates ABA signaling-related genes and stress response genes, and delays the upregulation of drought-inducible genes. These findings suggest that ethanol pretreatment improves drought avoidance in cassava through activation of the ABA signaling pathway, protein folding-related response, and changes in sugar and starch metabolism.
PLANT MOLECULAR BIOLOGY
(2022)
Article
Agronomy
Widiastuti Setyaningsih, Karmila, Rohmah Nur Fathimah, Muhammad Nur Cahyanto
Summary: Ultrasound-assisted extraction (UAE) has been optimized for cassava starch production, resulting in higher yield and lower viscosity compared to conventional methods. The Box-Behnken design combined with response surface methodology (RSM) effectively predicts and achieves the optimal extraction conditions, demonstrating the potential for starch modification to maintain viscosity at lower values.
Article
Agronomy
Xin Guo, Xiaohui Yu, Chenyu Lin, Pingjuan Zhao, Bin Wang, Liangping Zou, Shuxia Li, Xiaoling Yu, Yinhua Chen, Peng Zhang, Ming Peng, Mengbin Ruan
Summary: Under chilling stress, MeMYB2-RNAi transgenic cassava accumulated less ROS but more cyanidin-3-O-glucoside compared to the wild type. The anthocyanin biosynthesis pathway was more active in the transgenic cassava, and several genes, including MeTT8, were up-regulated. MeMYB2 bound to the MeTT8 promoter and inhibited its expression, thereby blocking anthocyanin accumulation. MeTT8 was shown to bind to the promoter of MeDFR-2 and increased its expression. MeMYB2 acts as an inhibitor of chilling-induced anthocyanin accumulation in cassava.
Review
Plant Sciences
Assefa B. Amelework, Michael W. Bairu
Summary: This article discusses the importance of cassava globally, particularly in Africa and South Africa. Due to its climate-resilient nature, cassava has become a popular crop among farming communities in frost-free areas of South Africa. A multidisciplinary research program has been established at the Agricultural Research Council of South Africa to promote progress in cassava breeding and genetic analysis.
Article
Multidisciplinary Sciences
Asunta Mukami, Bicko Steve Juma, Cecilia Mweu, Mathew Ngugi, Richard Oduor, Wilton Mwema Mbinda
Summary: This study evaluated factors influencing protoplast isolation, callus induction, and plant regeneration in cassava, and developed a simple and highly reproducible protocol.
Review
Biotechnology & Applied Microbiology
Egoamaka O. Egbune, Theresa Ezedom, Osuvwe C. Orororo, Olisemeke U. Egbune, Oghenetega J. Avwioroko, Eferhire Aganbi, Akpovwehwee A. Anigboro, Nyerhovwo J. Tonukari
Summary: Solid-state fermentation (SSF) is a promising technology for producing value-added products from cassava. It offers advantages such as lower costs, reduced water usage, and higher product yields. SSF can enhance the nutritional value of cassava-based products and minimize waste by utilizing cassava waste as a substrate. However, optimizing process conditions and developing cost-effective production processes are essential for successful commercialization.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Review
Engineering, Chemical
Elohor Oghenechavwuko Udoro, Tonna Ashim Anyasi, Afam Israel Obiefuna Jideani
Summary: Cassava flour, derived from the edible portion of highly perishable cassava root, is a shelf-stable food product categorized into fermented and unfermented types based on processing methods. The use of this flour is versatile, ranging from being consumed alone in dough form with soups to serving as a composite in various flour-based food products. Modifications in the processing of cassava flour have given rise to different types such as modified, unmodified, gelatinized, fortified, native, roasted, malted, wet, and dry, each with unique characteristics.
Article
Biochemistry & Molecular Biology
Chenyu Lin, Xin Guo, Xiaohui Yu, Shuxia Li, Wenbin Li, Xiaoling Yu, Feng An, Pingjuan Zhao, Mengbin Ruan
Summary: This study investigates the evolutionary relationships and characteristics of the RWP-RK family genes in cassava. The results show that these genes are unevenly distributed across the cassava genome and can be divided into two subfamilies. The analysis of RWP-RK promoters reveals the presence of many cis-acting elements related to hormones and stress response. Real-time quantitative PCR confirms that several NLP and RKD genes in cassava respond to various abiotic stressors, hormonal treatments, and nitrogen starvation. These findings provide a solid basis for further functional studies on RWP-RK genes in cassava.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Elza Aguirre, Jorge Dominguez, Eudes Villanueva, Juanita Anali Ponce-Ramirez, Maria de Fatima Arevalo-Oliva, Raul Siche, Jose Gonzalez-Cabeza, Gilbert Rodriguez
Summary: The study aims to develop biodegradable trays using cassava starch and corn husk flour through the thermoforming process. Physicochemical and mechanical analyses reveal that increased concentrations of corn husk flour improve tray properties. The blend of 85% cassava starch and 15% corn husk flour shows promising physical and mechanical properties for the formation of biodegradable trays. Further examination through XRD, TGA, FTIR, and SEM indicates the potential applications of these trays as packaging materials for various products.
FOOD PACKAGING AND SHELF LIFE
(2023)
Article
Biochemistry & Molecular Biology
Babak Behnam, Asuka Higo, Kaho Yamaguchi, Hiroki Tokunaga, Yoshinori Utsumi, Michael Gomez Selvaraj, Motoaki Seki, Manabu Ishitani, Luis Augusto Becerra Lopez-Lavalle, Hiroyuki Tsuji
Summary: This study characterized genes involved in the photoperiodic flowering pathway in cassava and identified two distinct developmental transitions in field-grown cassava. Cassava, an important crop for both edible and industrial purposes, develops storage roots that are a crucial staple food source in tropical regions.
PLANT MOLECULAR BIOLOGY
(2021)
Article
Genetics & Heredity
Tangwei Huang, Xinglu Luo, Zhupeng Fan, Yanni Yang, Wen Wan
Summary: This study identified and characterized seven non-redundant members of the SUS gene family in the cassava genome, showing their important roles in developmental stages, tissue expression, and stress responses.
Article
Plant Sciences
Liangping Zou, Dengfeng Qi, Shuxia Li, Min Zhai, Zhuang Li, Xin Guo, Mengbin Ruan, Xiaoling Yu, Pingjuan Zhao, Wenbin Li, Peng Zhang, Qiuxiang Ma, Ming Peng, Wenbin Liao
Summary: The function of MeNPF4.5, a plasma-membrane-localized gene in cassava, was investigated in this study. MeNPF4.5 acts as an efflux transporter for nitrate under low nitrate conditions, reducing the nitrate efflux capacity, but as an influx transporter under high nitrate conditions, increasing the nitrate absorption. Additionally, MeNPF4.5 negatively regulates osmotic stress and is involved in nitrate flux in cassava seedlings.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Yuwei Zang, Yechun Pei, Xinli Cong, Fangfang Ran, Liangwang Liu, Changyi Wang, Dayong Wang, Yi Min
Summary: This study created a single-cell transcriptome atlas of cassava leaves to investigate their structural and photosynthetic characteristics at the cellular level. The study identified three major tissues of cassava leaves and discovered genes related to cell differentiation fate.
Article
Biochemistry & Molecular Biology
Duan Wang, Xuan Zhang, Xiangmei Yao, Peng Zhang, Rongxiang Fang, Jian Ye
MOLECULAR PLANT-MICROBE INTERACTIONS
(2020)
Article
Biochemistry & Molecular Biology
Mengjiao Chen, Weijuan Fan, Feiyang Ji, Hua Hua, Jie Liu, Mengxiao Yan, Qingguo Ma, Jiongjiong Fan, Qin Wang, Shufeng Zhang, Guiling Liu, Zhe Sun, Changgeng Tian, Fengling Zhao, Jianli Zheng, Qi Zhang, Jiaxin Chen, Jie Qiu, Xin Wei, Ziru Chen, Peng Zhang, Dong Pei, Jun Yang, Xuehui Huang
Summary: The study introduced a method called OutcrossSeq for mapping important genes in outcrossing crops like sweet potato, walnut tree, and hybrids. By generating high-density genotype maps, the method enables precise identification of genomic loci underlying important agronomic traits and detection of potential causative genes based on functional clues. It is a robust and cost-efficient approach for identifying agronomically important genes in heterozygous species, including polyploids.
Article
Plant Sciences
Vikram Singh, Hanita Zemach, Sara Shabtai, Roni Aloni, Jun Yang, Peng Zhang, Lidiya Sergeeva, Wilco Ligterink, Nurit Firon
Summary: Sweetpotato is a globally important food crop, with its storage-root formation involving different developmental fates in different parts of the root, supported by distinct physiological, biochemical, and molecular mechanisms. Proximal root part exhibits lignin deposition, while the distal part demonstrates starch accumulation, with these differences further enhanced during storage-root formation.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Applied
Yongheng Zhong, Tao Xu, Xiaodan Wu, Kaimian Li, Peng Zhang, Shengyang Ji, Shimin Li, Lufei Zheng, Baiyi Lu
Summary: This paper evaluated the dietary risks of cassava cyanide and proposed a maximum residue limit for cyanogenic glycosides in cassava. The results showed no chronic health risk across all populations concerning cassava consumption. It is suggested that risk assessment of cyanide for foods rich in CNGs should be based on CNGs quantification rather than that of CN-.
Article
Plant Sciences
Weijuan Fan, Yandi Zhang, Yinliang Wu, Wenzhi Zhou, Jun Yang, Ling Yuan, Peng Zhang, Hongxia Wang
Summary: The IbVP1 gene plays a crucial role in regulating starch metabolism in sweet potato, with its overexpression enhancing photosynthesis ability, sucrose content, starch content, and total yield. It also promotes sucrose long-distance transport and enhances phosphate utilization efficiency, leading to increased starch content and changes in the structure, composition, and physicochemical properties of stored starch in sweet potato plants.
HORTICULTURE RESEARCH
(2021)
Article
Plant Sciences
Shutao He, Hongxia Wang, Xiaomeng Hao, Yinliang Wu, Xiaofeng Bian, Minhao Yin, Yandi Zhang, Weijuan Fan, Hao Dai, Ling Yuan, Peng Zhang, Luonan Chen
Summary: The study analyzed the dynamic gene regulatory network during sweet potato root development, revealing stage-specific expression patterns and key regulatory factors. It identified the early stage with a 3.5 mm diameter as the critical period for storage root swelling initiation, with the NAC transcription factor IbNAC083 playing a core regulatory role.
Article
Plant Sciences
Shutao He, Xiaomeng Hao, Shanshan Wang, Wenzhi Zhou, Qiuxiang Ma, Xinlu Lu, Luonan Chen, Peng Zhang
Summary: This study focuses on the starch synthase II in cassava storage roots and its impact on starch properties and the organization of starch synthase complexes. The findings reveal the important role of soluble starch synthase II in starch biosynthesis and formation of protein complexes with other biosynthetic enzymes. They also provide insights into the key components of starch biosynthesis in root crops.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Xiaoyun Wu, Jia Xu, Qiuxiang Ma, Sulaiman Ahmed, Xinlu Lu, Erjun Ling, Peng Zhang
Summary: This study reports the application of hen egg-white lysozyme (HEWL) in transgenic cassava to repress postharvest physiological deterioration. Lysozyme-expressing cassava showed lower levels of coumarins and increased tolerance of oxidative stress.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shu Luo, Qiuxiang Ma, Yingying Zhong, Jianling Jing, Zusheng Wei, Wenzhi Zhou, Xinlu Lu, Yinong Tian, Peng Zhang
Summary: The study achieved production of high-amylose cassava using CRISPR/Cas9 technology, leading to increased amylose and resistant starch content, altered starch properties, and potential applications in food and industrial sectors.
PLANT MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qiuxiang Ma, Jia Xu, Yancai Feng, Xiaoyun Wu, Xinlu Lu, Peng Zhang
Summary: The study demonstrates that MeC3'H is the key enzyme involved in coumarin biosynthesis during post-harvest physiological deterioration (PPD) of cassava storage roots. By repressing its expression, PPD can be significantly delayed, making MeC3'H a potential target gene for extending the shelf life of cassava storage roots.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Xin Guo, Xiaoling Yu, Ziyin Xu, Pingjuan Zhao, Liangping Zou, Wenbin Li, Mengting Geng, Peng Zhang, Ming Peng, Mengbin Ruan
Summary: In this study, we identified a drought-responsive CC-type GRX, MeGRXC3, in cassava and demonstrated its role in regulating activity of catalase and stomatal movement under drought stress. Our findings provide insights into the potential application of MeGRXC3 in molecular breeding of cassava crops.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Plant Sciences
Liangping Zou, Dengfeng Qi, Shuxia Li, Min Zhai, Zhuang Li, Xin Guo, Mengbin Ruan, Xiaoling Yu, Pingjuan Zhao, Wenbin Li, Peng Zhang, Qiuxiang Ma, Ming Peng, Wenbin Liao
Summary: The function of MeNPF4.5, a plasma-membrane-localized gene in cassava, was investigated in this study. MeNPF4.5 acts as an efflux transporter for nitrate under low nitrate conditions, reducing the nitrate efflux capacity, but as an influx transporter under high nitrate conditions, increasing the nitrate absorption. Additionally, MeNPF4.5 negatively regulates osmotic stress and is involved in nitrate flux in cassava seedlings.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Qiuxiang Ma, Yancai Feng, Shu Luo, Lu Cheng, Weijing Tong, Xinlu Lu, Youzhi Li, Peng Zhang
Summary: Aquaporins, particularly MePIP2;7, play a crucial role in the synergistic transport of Mg-2(+) and water in cassava. MePIP2;7 enhances the transportability of Mg-2(+) by interacting with MeMGT9, a magnesium transporter protein, thereby regulating the absorption and transport of water and Mg(2)(+) in cassava.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Agronomy
Xin Guo, Xiaohui Yu, Chenyu Lin, Pingjuan Zhao, Bin Wang, Liangping Zou, Shuxia Li, Xiaoling Yu, Yinhua Chen, Peng Zhang, Ming Peng, Mengbin Ruan
Summary: Under chilling stress, MeMYB2-RNAi transgenic cassava accumulated less ROS but more cyanidin-3-O-glucoside compared to the wild type. The anthocyanin biosynthesis pathway was more active in the transgenic cassava, and several genes, including MeTT8, were up-regulated. MeMYB2 bound to the MeTT8 promoter and inhibited its expression, thereby blocking anthocyanin accumulation. MeTT8 was shown to bind to the promoter of MeDFR-2 and increased its expression. MeMYB2 acts as an inhibitor of chilling-induced anthocyanin accumulation in cassava.
Review
Biochemistry & Molecular Biology
Ruiqing Lyu, Sulaiman Ahmed, Weijuan Fan, Jun Yang, Xiaoyun Wu, Wenzhi Zhou, Peng Zhang, Ling Yuan, Hongxia Wang
Summary: Sweet potato is a significant food crop with valuable starch content and strong adaptability to environmental stresses caused by climate change. The complexity of the sweet potato genome and long breeding cycle limit our ability to modify starch properties. Biotechnological methods, such as CRISPR/Cas-based genome editing, offer new opportunities to optimize the starch properties of sweet potatoes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)