Review
Biotechnology & Applied Microbiology
Qian Zhang, Yixin Hu, Xuan Long, Lingling Hu, Yu Wu, Ji Wu, Xiaobing Shi, Runqi Xie, Yu Bi, Fangyuan Yu, Pinxue Li, Yu Yang
Summary: Cartilage regeneration relies on cellular-ECM interactions. Traditional tissue engineering methods have not been able to replicate the physiological structure of natural cartilage, but recent advances in ECM-based scaffolds show promise in improving cartilage regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ying Zhang, Chenyu Zhang, Yuwen Li, Lingyan Zhou, Nianhua Dan, Jie Min, Yining Chen, Yunbing Wang
Summary: Tissue engineering is a technique that mimics nature by using biomimetic ECM scaffolds. Among these scaffolds, the decellularized ECM scaffolds (dECMs) stand out for their natural components and microenvironment. This article provides an overview of the family of dECMs and discusses various decellularization technologies. It also reviews recent advances and applications of dECMs in different fields, and outlines the development directions of dECMs in tissue engineering. dECMs continue to present challenges and opportunities for regenerative medicine.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Biomedical
Katsuhiro Tomofuji, Ken Fukumitsu, Jumpei Kondo, Hiroshi Horie, Kenta Makino, Satoshi Wakama, Takashi Ito, Yu Oshima, Satoshi Ogiso, Takamichi Ishii, Masahiro Inoue, Etsuro Hatano
Summary: In this study, researchers reconstructed intrahepatic bile ducts in a rat decellularized liver graft using organoid technology. Through an ex vivo perfusion culture system, they successfully demonstrated the biliary characteristics of repopulated liver organoids and reconstructed biliary tree-like networks with luminal structures. They also established a method to mimic the appropriate cell distribution in the native liver and demonstrated reconstructed bile ducts in human organoids.
Article
Engineering, Biomedical
Charlot Philips, Lisanne Terrie, Lieven Thorrez
Summary: A review is presented on the progress and challenges in skeletal muscle decellularization, discussing different decellularization methods and applications of the scaffolds. Decellularized skeletal muscle has been primarily studied for regenerative purposes, focusing on tissue-specific morphological features and biochemical cues for muscle regeneration. However, this review expands the potential applications of decellularized skeletal muscle to demonstrate its versatility as a biomaterial.
Review
Biochemistry & Molecular Biology
Fouad Al-Hakim Khalak, Fatima Garcia-Villen, Sandra Ruiz-Alonso, Jose Luis Pedraz, Laura Saenz-del-Burgo
Summary: In recent years, there has been a growing demand for improving the regeneration of damaged tendons. Current treatments using grafts have limitations as they cannot provide sufficient support for tissue regeneration. A recent study has highlighted the potential benefits of combining 3D bioprinting and dECM as a novel bioink biomaterial. However, there are still challenges to overcome, such as the poor structural integrity and slow gelation properties of dECM bioinks.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Silvia Barbon, Elena Stocco, Martina Contran, Federico Facchin, Rafael Boscolo-Berto, Silvia Todros, Deborah Sandrin, Filippo Romanato, Piero Pavan, Veronica Macchi, Vincenzo Vindigni, Franco Bassetto, Raffaele De Caro, Andrea Porzionato
Summary: This study considers the use of cellular human diaphragm as a scaffold material for the treatment of volumetric muscle loss (VML). After decellularization through four detergent-enzymatic protocols, cells, DNA, and muscle fibers were effectively removed, while collagen/elastin and the majority of the glycosaminoglycan component were preserved. Adipose-derived stem cells cultured with decellularized samples demonstrated high viability, indicating the biosafety of the scaffolds. Acellular diaphragmatic grafts treated with SDS+Tergitol (TM) showed better collagen preservation and integrated well with host tissue upon implantation in mice.
Article
Biochemistry & Molecular Biology
Constanze Kuhlmann, Thilo L. Schenck, Attila Aszodi, Riccardo E. Giunta, Paul Severin Wiggenhauser
Summary: This study investigated the influence of different zones in human nasal septal cartilage on the fate of adipose tissue-derived stem cells. The findings suggest that the zonal-dependent architecture and composition of nasal cartilage modulate the activities of seeded stem cells, providing new insights for engineering cartilage substitutes in facial reconstructive surgery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Rui Zhang, Junqun Jiang, Yaling Yu, Fangfang Wang, Niuniu Gao, Yingjie Zhou, Xinlong Wan, Zhibin Wang, Peng Wei, Jin Mei
Summary: Chronic kidney disease is a major global public health problem, with renal fibrosis being a common pathological process in chronic renal failure. Research indicates that renal fibrosis decellularized scaffolds have the potential to serve as a model for cellular mechanisms of tissue fibrosis or donors for tissue engineering. Analysis through histology, proteomics, and ELISA showed changes in the structure and components of fibrotic tissue. The fibrosis model induced by adenine-fed demonstrated changes in the fibrotic scaffold's structure and protein expression, but cytokines associated with renal regeneration post-injury were maintained, indicating potential for tissue engineering.
BIOACTIVE MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Ifeolu Akinnola, Daniel R. Rossi, Carolyn Meyer, Ashley Lindsey, Douglas R. Haase, Samuel Fogas, Michael J. Ehrhardt, Rachel E. Blue, Andrew P. Price, Max Johnson, Diego F. Alvarez, Doris A. Taylor, Angela Panoskaltsis-Mortari
Summary: This study evaluated the recellularization of blood vessel conduits of acellular lung scaffolds with different types of endothelial cells, showing that RMEPCs have the capability to repopulate all vascular segments, while PAECs and MVECs exhibit selective tropism for larger vessels or microvasculature, respectively. The RMEPC phenotype described in the study could be valuable for identifying suitable endothelial progenitors for efficient vascular organ and tissue engineering, regeneration, and repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Daniele Boso, Eugenia Carraro, Edoardo Maghin, Silvia Todros, Arben Dedja, Monica Giomo, Nicola Elvassore, Paolo De Coppi, Piero Giovanni Pavan, Martina Piccoli
Summary: Hydrogels, particularly those derived from decellularized extracellular matrix (dECM), show superior properties in mimicking native tissue composition and promoting tissue regeneration. A porcine diaphragmatic dECM-derived hydrogel has been developed and characterized for diaphragmatic applications, demonstrating potential as a useful biological product for diaphragmatic muscle defect repair.
Article
Anatomy & Morphology
Aida Nahumi, Maryam Peymani, Asadollah Asadi, Arash Abdolmaleki, Yassin Panahi
Summary: Tissue engineering research has focused on the characteristics and biocompatibility of decellularized sheep tracheal scaffolds, as well as the differentiation of Adipose-derived stem cells (AD-MSCs) into tracheal cells. The prepared scaffolds show potential for engineering applications and provide a suitable environment for stem cell differentiation. This research serves as a foundation for future studies on tracheal decellularization scaffolds and paves the way for their use in organ regeneration and clinical medicine.
Review
Biochemistry & Molecular Biology
Chao Liang, Li Liao, Weidong Tian
Summary: Decellularized extracellular matrix (dECM) promotes stem cell proliferation, migration, adhesion, and differentiation, making it a promising biomaterial for periodontal tissue engineering. It effectively preserves ECM components as they are in native tissue, providing ideal cues for regeneration and repair. Different sources of dECM have different advantages in promoting periodontal tissue regeneration. Various methods have been developed to improve the mechanical strength of dECM, such as functionalized scaffolds and crosslinked soluble dECM. dECM has been successful in periodontal regeneration therapies and holds potential for future applications.
Article
Biochemistry & Molecular Biology
Jie Xu, Huan Fang, Shuangshuang Zheng, Liying Li, Zeren Jiao, Hong Wang, Yi Nie, Tianqing Liu, Kedong Song
Summary: A novel dECM/Gel/CS scaffold was successfully prepared with appropriate mechanical strength, good antibacterial activity, and high biocompatibility, showing potential for promoting cell growth and new tissue formation in skin tissue engineering. The presence of dECM effectively enhanced cell proliferation, making the scaffold a promising candidate for use in skin tissue engineering applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Flavia Carton, Dalila Di Francesco, Luca Fusaro, Emma Zanella, Claudio Apostolo, Francesca Oltolina, Diego Cotella, Maria Prat, Francesca Boccafoschi
Summary: The study evaluated the myogenic potential of dECM substrate derived from decellularized bovine pericardium on murine muscle cells and found that dECM could support and enhance the myogenic potential of muscle cells compared to cells grown on a standard plastic surface. This suggests that extracellular matrix proteins could be a promising therapeutic strategy for skeletal muscle tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Wonbin Park, Ge Gao, Dong-Woo Cho
Summary: This review discusses the advances in musculoskeletal tissue engineering using 3D bioprinting technology and decellularized extracellular matrix bioink. It focuses on in vivo regeneration and in vitro modeling of musculoskeletal tissue, while also addressing current breakthroughs, limitations, and future prospects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Developmental Biology
P. Murthi, D. E. van Zanten, J. J. H. Eijsink, A. J. Borg, J. L. Stevenson, B. Kalionis, A. K. Chui, J. M. Said, S. P. Brennecke, J. J. H. M. Emrich
Article
Developmental Biology
Balta Al-Sowayan, Rosemary J. Keogh, Mohammed Abumaree, Harry M. Georgiou, Bill Kalionis
Article
Developmental Biology
M. H. Abumaree, F. M. Abomaray, M. A. Alshabibi, A. S. AlAskar, B. Kalionis
Review
Biochemistry & Molecular Biology
Lumei Liu, Jian Wang, Ramin Khanabdali, Bill Kalionis, Xiantao Tai, Shijin Xia
Review
Materials Science, Biomaterials
I. M. Bjorge, S. Y. Kim, J. F. Mano, B. Kalionis, W. Chrzanowski
BIOMATERIALS SCIENCE
(2017)
Article
Cell & Tissue Engineering
T. Khatlani, D. Algudiri, R. Alenzi, A. M. Al Subayyil, F. M. Abomaray, E. Bahattab, A. S. Alaskar, B. Kalionis, M. F. El-Muzaini, M. H. Abumaree
STEM CELLS INTERNATIONAL
(2018)
Article
Chemistry, Physical
Sally Yunsun Kim, Dipesh Khanal, Priyanka Tharkar, Bill Kalionis, Wojciech Chrzanowski
NANOSCALE HORIZONS
(2018)
Article
Genetics & Heredity
Ramin Khanabdali, Aida Shakouri-Motlagh, Sarah Wilkinson, Padma Murthi, Harry M. Georgiou, Shaun P. Brennecke, Bill Kalionis
JOURNAL OF MOLECULAR MEDICINE-JMM
(2018)
Article
Cell Biology
Mohamed H. Abumaree, Seham Al Harthy, Abdullah M. Al Subayyil, Manal A. Alshabibi, Fawaz M. Abomaray, Tanvier Khatlani, Bill Kalionis, Mohammed F. El-Muzaini, Mohammed A. Al Jumah, Dunia Jawdat, Abdullah O. Alawad, Ahmed S. AlAskar
Review
Reproductive Biology
Joan C. Wijaya, Ramin Khanabdali, Harry M. Georgiou, Bill Kalionis
BIOLOGY OF REPRODUCTION
(2020)
Article
Developmental Biology
C. Perera, S. Zheng, M. I. Kokkinos, H. M. Georgiou, M. Schoppet, P. F. James, S. P. Brennecke, B. Kalionis
Summary: In preeclampsia, the presence of PE-DMSCs in the vascular niche contributes to endothelial cell dysfunction due to increased release of ENG. Conversely, in normotensive pregnancies, DMSC secretion of ENG likely has a beneficial effect on endothelial cells.
Article
Developmental Biology
Joan C. Wijaya, Ramin Khanabdali, Harry M. Georgiou, Maria Kokkinos, Patrick F. James, Shaun P. Brennecke, Bill Kalionis
MOLECULAR HUMAN REPRODUCTION
(2020)
Article
Obstetrics & Gynecology
Alice F. Wookey, Tejasvy Chollangi, Hannah E. J. Yong, Bill Kalionis, Shaun P. Brennecke, Padma Murthi, Harry M. Georgiou
JOURNAL OF PREGNANCY
(2017)