Article
Biochemistry & Molecular Biology
Elia Bari, Ilaria Roato, Giuseppe Perale, Filippo Rossi, Tullio Genova, Federico Mussano, Riccardo Ferracini, Marzio Sorlini, Maria Luisa Torre, Sara Perteghella
Summary: A new strategy was developed to improve the osteoinductivity of SmartBone (SB) by loading the SB scaffolds with freeze-dried formulation of mesenchymal stem cell (MSC)-secretome. The study showed that SBlyo significantly enhanced cell proliferation and tissue formation compared to SB, suggesting a more effective bone formation induction by SBlyo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jieun Baek, Bokyeong Ryu, Jin Kim, Seul-Gi Lee, Min-Seok Oh, Ki-Sung Hong, Eun-Young Kim, C-Yoon Kim, Hyung-Min Chung
Summary: This study compared M-MSCs with adult-MSCs and found that M-MSCs have the potential to be used in next-generation therapies for rotator cuff tears (RCTs). They are able to regulate inflammation and promote tissue repair, leading to improved muscle recovery in RCTs.
Article
Chemistry, Multidisciplinary
Heqi Xu, Jiachen Liu, Md Shahriar, Changxue Xu
Summary: This study aims to investigate and quantify cell aggregation and its effects on the droplet formation process and cell distribution during inkjet-based bioprinting. The major conclusions are as follows: (1) Cell aggregation is a significant challenge in inkjet-based bioprinting, and it is suppressed as polymer concentration increases. (2) The length of ligaments and droplet velocity initially decrease and then increase as printing time and cell aggregation increase due to the viscosity effect. (3) With longer printing time, both the maximum number of cells within one microsphere and the mean cell number significantly increase, especially at low polymer concentrations like 0.5% (w/v) due to higher cell sedimentation velocity.
Review
Cell & Tissue Engineering
Mengqin Yuan, Xue Hu, Lichao Yao, Yingan Jiang, Lanjuan Li
Summary: Mesenchymal stem cell (MSC) transplantation is an effective strategy for treating end-stage liver disease by regenerating tissues and self-renewing to repair the liver. However, the low homing efficiency of MSCs after systemic administration is a major limitation in its application for liver disease treatment.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Elia Bari, Fulvio Tartara, Fabio Cofano, Giuseppe di Perna, Diego Garbossa, Sara Perteghella, Marzio Sorlini, Delia Mandracchia, Lorella Giovannelli, Paolo Gaetani, Maria Luisa Torre, Lorena Segale
Summary: The study found that the mesenchymal stem cell-secretome Lyosecretome has a significant improvement effect on the osteoinductive and osteoconductive properties of titanium cages, leading to increased mineralized matrix when used during culturing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
Dimitrios Kouroupis, Diego Correa
Summary: The detrimental effects of conventional 2D MSC expansion have led to the development of novel techniques, such as 3D culturing, to enhance the functionality and efficacy of MSCs. MSC 3D spheroids exhibit enhanced medicinal signaling activities and improved homing and survival capacities, demonstrating safety and efficacy in preclinical studies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Health Care Sciences & Services
Aigul R. Rakhmatullina, Rimma N. Mingaleeva, Dina U. Gafurbaeva, Olesya N. Glazunova, Aisylu R. Sagdeeva, Emil R. Bulatov, Albert A. Rizvanov, Regina R. Miftakhova
Summary: This study successfully immortalized adipose-derived MSCs by overexpressing hTERT gene and knocking down TP53, maintaining the differentiation potential and cell morphology stability during long-term culture.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Oncology
Khaled Zohni, Lianet Lopez, Poonam Mander, Peter Szaraz, Melissa Filice, Brandon A. Wyse, Meredith Garcia, Itai Gat, Karen Glass, Andree Gauthier-Fisher, Clifford L. Librach
Summary: Chemotherapies can lead to germ cell depletion and gonadal failure. In this study, first trimester human umbilical cord perivascular cells (HUCPVCs) were shown to resist the cytotoxic effects of cyclophosphamide and prevent ovarian damage in rodent models of chemotherapy-induced gonadal injury. These cells demonstrate promising potential for fertility preservation.
Review
Biochemistry & Molecular Biology
Ernest Lee, Ilias Ektor Epanomeritakis, Victor Lu, Wasim Khan
Summary: Osteoarthritis is a long-term consequence of focal cartilage defects of the knee and has led to the exploration of new therapies for cartilage regeneration. Recent studies have investigated the use of mesenchymal stem cells (MSCs) and polymer scaffold compositions. Implants seeded with bone marrow-derived MSCs (BMSCs) have shown promising results in restoring these defects, with high-quality integration and repair cartilage that resembles native cartilage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Raul Lopez, Gerardo J. Marti-Chillon, Juan F. Blanco, Carmen da Casa, Javier Gonzalez-Robledo, David Pescador, Silvia Preciado, Sandra Muntion, Fermin Sanchez-Guijo
Summary: The study showed that MSCs from polytrauma patients have higher proliferative potential and early pre-osteogenic differentiation ability. This suggests that MSCs could potentially aid in the repair process of polytrauma patients and contribute to both cell-tissue repair and anti-inflammatory response. Further exploration of this potential in larger studies is warranted.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Orthopedics
Shipin Zhang, Keng Lin Wong, Xiafei Ren, Kristeen Ye Wen Teo, Hassan Afizah, Andre Boon Hwa Choo, Ruenn Chai Lai, Sai Kiang Lim, James Hoi Po Hui, Wei Seong Toh
Summary: This study validates the safety and efficacy of human mesenchymal stem cell (MSC) exosomes combined with hyaluronic acid (HA) for osteochondral repair in a clinically relevant micropig model. The results show that the combination treatment promotes functional cartilage and subchondral bone repair, with significantly improved morphological, histological, and biomechanical outcomes. No adverse response or major systemic alterations were observed, indicating the potential of this therapy for clinical applications.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2022)
Article
Cell Biology
Meng Feng, Wenguang Liu, Jing Ding, Yusheng Qiu, Qian Chen
Summary: Hedgehog (HH) signaling is critical in osteoarthritis (OA) pathogenesis, mediating the interaction between OA mesenchymal stromal cells (OA-MSCs) and chondrocytes. Sonic Hedgehog (SHH) acts as a key mediator in promoting MSC growth, aging, low-grade inflammation, replicative senescence, and inducing OA-MSC-induced chondrocyte catabolism and apoptosis through a pro-inflammatory microenvironment in human OA cartilage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Multidisciplinary Sciences
Luisa Nathalia Fonseca, Santiago Bolivar-Mona, Tatiana Agudelo, Liz Daniela Beltran, Daniel Camargo, Nestor Correa, Maria Alexandra Del Castillo, Sebastian Fernandez de Castro, Valeria Fula, Gabriela Garcia, Natalia Guarnizo, Valentina Lugo, Liz Mariana Martinez, Veronica Melgar, Maria Clara Pena, Wilfran Arbey Perez, Nicolas Rodriguez, Andres Pinzon, Sonia Luz Albarracin, Mercedes Olaya, Maria Lucia Gutierrez-Gomez
Summary: This study reviewed the literature to determine the surface markers for human MSCs associated with skeletal tissue. The most widely used markers were CD105, CD90, and CD73, but they do not represent true stemness. Most studies did not evaluate the characteristics of self-renewal and differentiation, which are necessary to distinguish between stem cells and progenitor populations.
Article
Biochemistry & Molecular Biology
Zun Wang, Xiaohua Li, Junxiao Yang, Yun Gong, Huixi Zhang, Xiang Qiu, Ying Liu, Cui Zhou, Yu Chen, Jonathan Greenbaum, Liang Cheng, Yihe Hu, Jie Xie, Xucheng Yang, Yusheng Li, Martin R. Schiller, Yiping Chen, Lijun Tan, Si-Yuan Tang, Hui Shen, Hong-Mei Xiao, Hong-Wen Deng
Summary: This study utilized single-cell RNA sequencing to identify distinct subpopulations of BM-MSCs in humans, shedding light on their cellular heterogeneity and roles in maintaining bone homeostasis, as well as potential novel markers for BM-MSC purification.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Review
Cell & Tissue Engineering
Ahmed Lotfy, Noha M. AboQuella, Hongjun Wang
Summary: Mesenchymal stromal/stem cells (MSCs) are widely used in cell therapy due to their immunomodulatory and regenerative properties. The production of various factors via extracellular vesicles, especially exosomes, governs the efficacy of MSCs. MSC-derived exosomes (MSC-Exos) show advantages over MSCs themselves by reducing adverse effects. Clinical studies are assessing the therapeutic effect of MSC-Exos in different diseases, and production methods, dosage, and administration routes are being explored.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Orthopedics
L. Z. Yapp, N. D. Clement, M. Moran, J. Clarke, A. H. R. W. Simpson, C. E. H. Scott
Summary: This study aimed to determine the long-term mortality rate and associated factors following primary and revision knee arthroplasty. Retrospective analysis of data from the Scottish Arthroplasty Project revealed that the mortality rates for both primary and revision cohorts were lower than the general population. However, certain patient and surgical factors, such as periprosthetic joint infection (PJI), were associated with an increased risk of mortality within ten years post-surgery.
BONE & JOINT JOURNAL
(2022)
Editorial Material
Cell & Tissue Engineering
G. T. Snowden, N. D. Clement, S. Zhang, Q. Xue, A. H. R. W. Simpson
BONE & JOINT RESEARCH
(2022)
Article
Orthopedics
Marc R. Safran, Iain R. Murray, Antonio J. Andrade, Stephen K. Aoki, Olufemi R. Ayeni, Jitendra Balakumar, Nicolas Bonin, Matthew J. Brick, Josip N. Cakic, Benjamin G. Domb, Richard E. Field, Frederic Laude, Omer Mei-Dan, Shane J. Nho, John M. O'Donnell, Marc J. Philippon, Vikas Khanduja
Summary: The study aimed to establish an international expert consensus on operating room findings that aid in the diagnosis of hip instability. By reviewing the literature and conducting expert discussions, 8 criteria were identified as helpful in confirming the diagnosis of hip instability.
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
(2022)
Article
Orthopedics
N. D. Clement, M. S. Gaston, A. H. Simpson
Summary: The objective of this study was to assess the cellular age-related changes in fracture repair and relate these to the observed radiographic assessments at differing time points. The results showed that elderly mice demonstrated a delay in endochondral ossification which was associated with a decreased relative bone mineral content at the fracture site.
EUROPEAN JOURNAL OF ORTHOPAEDIC SURGERY AND TRAUMATOLOGY
(2023)
Review
Orthopedics
F. Brzeszczynski, J. Brzeszczynska, A. D. Duckworth, I. R. Murray, A. H. R. W. Simpson, D. F. Hamilton
Summary: This systematic review evaluated the effects of sarcopenia on postoperative functional recovery and mortality in orthopaedic surgery, and assessed the methods used to diagnose and define sarcopenia. The results showed that sarcopenia has a negative impact on surgical outcomes and further research is needed to improve diagnosis and interventions for sarcopenic patients.
BONE & JOINT JOURNAL
(2022)
Article
Cell & Tissue Engineering
S. M. Graham, M. M. K. Jalal, D. G. Lalloo, A. Hamish R. W. Simpson
Summary: This study evaluated the effect of combination antiretroviral therapy (ART) on fracture healing. The results showed that ART treatment led to delayed fracture healing at week 4 after surgery in rats, but did not result in nonunion.
BONE & JOINT RESEARCH
(2022)
Article
Cell & Tissue Engineering
R. G. E. Clement, A. C. Hall, S. J. Wong, S. E. M. Howie, A. H. R. W. Simpson
Summary: This study compared the effects of a haemolysin and the immune response on chondrocyte death in a murine joint model. The results showed that the haemolysin was the major cause of chondrocyte death, and the immune system also had a smaller deleterious role on cartilage.
BONE & JOINT RESEARCH
(2022)
Article
Cell & Tissue Engineering
L. Z. Yapp, C. E. H. Scott, C. R. Howie, D. J. MacDonald, A. H. R. W. Simpson, N. D. Clement
Summary: This study aimed to report the meaningful values of EQ-5D-3L questionnaire and EQ-VAS in patients undergoing primary knee arthroplasty. The study found that EQ-5D-3L questionnaire demonstrated good internal consistency and determined the minimum clinically important difference for evaluating clinically relevant changes. The findings of this study are of significance for assessing postoperative changes in patients' quality of life.
BONE & JOINT RESEARCH
(2022)
Article
Orthopedics
L. Z. Yapp, N. D. Clement, M. Moran, J. Clarke, A. H. R. W. Simpson, C. E. H. Scott
Summary: This study aimed to assess factors associated with the estimated lifetime risk of revision surgery after primary knee arthroplasty (KA). The results showed that patient sex, age, and underlying diagnosis were related to the risk of revision surgery.
BONE & JOINT JOURNAL
(2022)
Review
Sport Sciences
Navnit S. Makaram, Iain R. Murray, Andrew G. Geeslin, Jorge Chahla, Gilbert Moatshe, Robert F. LaPrade
Summary: This scoping review maps the current literature on the diagnosis and treatment of multiligament knee injuries (MLKIs). The findings reveal a lack of high-level evidence to support definitive management strategies for MLKI, as well as considerable heterogeneity and a need for minimum reporting standards in the current literature.
BRITISH JOURNAL OF SPORTS MEDICINE
(2023)
Article
Critical Care Medicine
Navnit S. Makaram, Andrew J. Hall, Nicholas D. Clement, Alasdair J. MacLullich, A. Hamish R. W. Simpson
Summary: Hip fracture is a common and serious orthopaedic injury among older people. In Scotland, the number of hip fractures is expected to rise from 7,000 to 10,300 per year by 2029. This narrative review discusses the Scottish Hip Fracture Audit, its impact on improving hip fracture care and outcomes, and the current status of hip fracture registries around the world. Future directions for improving hip fracture care through data collection and standardization are also explored.
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
(2023)
Editorial Material
Cell & Tissue Engineering
A. H. R. W. Simpson, N. S. Makaram, E. Harrison, J. Norrie
BONE & JOINT RESEARCH
(2023)
Article
Orthopedics
L. Z. Yapp, C. E. H. Scott, D. J. MacDonald, C. R. Howie, A. H. R. W. Simpson, N. D. Clement
Summary: This study aimed to investigate whether primary knee arthroplasty (KA) restores health-related quality of life (HRQoL) to levels expected in the general population. Using retrospective case-control design, HRQoL data from patients undergoing KA were compared with data from the Health Survey for England. The results showed that patients awaiting KA had poorer HRQoL compared to the general population, but one year after surgery, their HRQoL improved significantly and reached levels similar to their matched peers.
BONE & JOINT JOURNAL
(2023)
Article
Orthopedics
Thomas R. Williamson, Patrick G. Robinson, Iain R. Murray, Andrew D. Murray, Julie M. McBirnie, C. Michael Robinson, Deborah J. MacDonald, Nicholas D. Clement
Summary: This study aimed to determine the level of return to golf following rotator cuff repair, the barriers to return, and factors associated with failure to return. The results showed that 76.3% of patients successfully returned to golf, mostly to their previous performance level, and had improved quality of life. Persistent shoulder dysfunction was the primary barrier to return.
CLINICS IN SHOULDER AND ELBOW
(2023)
Article
Orthopedics
William M. Oliver, Jamie A. Nicholson, Katrina R. Bell, Thomas H. Carter, Timothy O. White, Nicholas D. Clement, Andrew D. Duckworth, A. Hamish R. W. Simpson
Summary: This study aimed to assess the feasibility and reliability of ultrasound in evaluating humeral shaft fracture healing, as well as the accuracy of 6-week ultrasound in predicting nonunion. The results showed that ultrasound assessment of humeral shaft fracture healing was feasible, reliable, and may predict nonunion.
EUROPEAN JOURNAL OF ORTHOPAEDIC SURGERY AND TRAUMATOLOGY
(2023)