Journal
PHARMACEUTICAL BIOLOGY
Volume 55, Issue 1, Pages 1545-1552Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/13880209.2017.1309554
Keywords
Duck hepatitis A virus type 1; baicalin-linarinicariin-notoginsenoside R1; virus adsorption; virus replication; virus release
Funding
- National Natural Science Foundation of China [31172355, 31572557]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Special Fund for Agro-scientific Research in the Public Interest [201303040, 201403051]
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Context: The flavonoid prescription baicalin-linarin-icariin-notoginsenoside R1 (BLIN) has a curative effect on duck virus hepatitis (DVH) caused by duck hepatitis A virus type 1 (DHAV-1). However, the mechanism of this curative effect is not understood. Objective: This study investigates the mechanism of the curative effect of BLIN on DVH caused by DHAV-1. We analyzed the anti-DHAV-1 reproduction mechanism and immuno-regulatory effect of BLIN. Materials and methods: The anti-DHAV-1 reproduction effects of BLIN at 20, 10, 5 and 2.5 mu g/mL in vitro, as well as the influence of BLIN at 20 mu g/mL on DHAV-1 adsorption, replication and release were tested using the qRT-PCR method. The promotion abilities of BLIN at 20, 10, 5 and 2.5 mu g/mL on T- and B-lymphocyte proliferation were investigated by the MTT method. IL-2 and IFN-gamma levels and total anti-DHAV-1 antibody secretion after treatment with DHAV-1 for 4, 8 and 54 h were determined by ELISA. Results: BLIN showed a dose-dependent DHAV-1 reproduction inhibitory effect. The inhibitory effect was highest at 20 mu g/mL, where DHAV-1 adsorption and release were significantly lower. Meanwhile, BLIN at 5 mu g/mL significantly increased T and B lymphocyte proliferation. BLIN stimulated total anti-DHAV-1 antibody secretion in ducklings at the dosage of 4mg per duckling, but did not stimulate IL-2 and IFN-gamma secretion significantly. Conclusions: BLIN inhibits DHAV-1 reproduction by suppressing its adsorption and release. Additionally, BLIN promoted the duckling antiviral response.
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