Article
Biology
Dibesh Thapa, Joao de Sousa Valente, Brentton Barrett, Matthew John Smith, Fulye Argunhan, Sheng Y. Lee, Sofya Nikitochkina, Xenia Kodji, Susan D. Brain
Summary: Ageing is associated with increased vulnerability to environmental cold exposure, leading to impaired behavioural and vascular responses to skin local environmental cooling in aged mice. The decline in TRPM8 gene/protein expression in ageing mice suggests a reliance on remaining TRP receptor activity for cold-induced vascular response. Sympathetic-induced vasoconstriction is also reduced with aging, indicating a decline in the cold-induced vascular response necessary for maintaining body heat and health.
Article
Medicine, Research & Experimental
Sabrina Qader Kudsi, Bruna Candia Piccoli, Daniel Ardisson-Araujo, Gabriela Trevisan
Summary: This article aims to analyze the baseline distribution of TRPA1, TRPV1, TRPV4, and TRPM8 channels in human systems at the transcriptional level. Using the RNA-seq dataset, the transcriptional levels of these channels were investigated and compared in 95 human subjects representing 33 different tissues, providing essential suggestions for further studies on these proteins.
Article
Biology
Pratish Thakore, Michael G. Alvarado, Sher Ali, Amreen Mughal, Paulo W. Pires, Evan Yamasaki, Harry A. T. Pritchard, Brant E. Isakson, Cam Ha T. Tran, Scott Earley
Summary: Cerebral blood flow is dynamically regulated by neurovascular coupling to meet the brain's metabolic demands. TRPA1 channels in capillary endothelial cells act as neural activity sensors, initiating microvascular vasodilatory responses to redirect blood flow to regions of metabolic demand.
Article
Neurosciences
Chenyu Yang, Shanni Yamaki, Tyler Jung, Brian Kim, Ryan Huyhn, David D. McKemy
Summary: The detection of environmental temperatures is crucial for survival and inappropriate responses to thermal stimuli can negatively affect overall health. The physiological effect of cold is distinct and can be soothing and analgesic, but also agonizing in the context of tissue damage. Inflammatory mediators induce neurogenic inflammation that alters cold sensitivity through the activation of TRPV1 and TRPA1 ion channels.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Andrea Gyenes, Zsofia Tapaszto, Susana Quirce, Carolina Luna, Laura Frutos-Rincon, Juana Gallar, M. Carmen Acosta, Illes Kovacs
Summary: CsA has good clinical results in treating dry eye by improving tear film dynamics and reducing subjective symptoms. It can directly affect sensory nerve endings, decreasing the peak response to cold in corneal thermoreceptors without modifying their spontaneous discharge rate or cooling threshold. Further studies are needed to fully understand the underlying mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mariana A. Freitas, Amanda Vasconcelos, Elaine C. D. Goncalves, Eduarda G. Ferrarini, Gabriela B. Vieira, Donatella Cicia, Maira Cola, Raffaele Capasso, Rafael C. Dutra
Summary: Spirulina platensis is a super-food with anti-inflammatory, antioxidant, and analgesic properties, showing significant analgesic effects in rodent models of inflammatory pain by potentially modulating TRMP8 and TRPA1.
Article
Biochemistry & Molecular Biology
Leevi Halonen, Antti Pemmari, Elina Nummenmaa, Mari Haemaelaeinen, Teemu Moilanen, Katriina Vuolteenaho, Eeva Moilanen
Summary: This study found that human osteoarthritic chondrocytes express 19 different TRP genes, with significant expression of TRPM8. In addition, menthol and dexamethasone can regulate the expression of TRP channels. These results support the concept of TRPA1 and TRPM8 as potential novel drug targets in arthritis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Xiancui Lu, Zhihao Yao, Yunfei Wang, Chuanlin Yin, Jiameng Li, Longhui Chai, Wenqi Dong, Licheng Yuan, Ren Lai, Shilong Yang
Summary: Molecular thermosensors in animals, specifically the TRPM8 gene, play a critical role in accurately sensing and responding to ambient temperature fluctuations. Through the process of evolution, the TRPM8 gene has evolved to form and regulate different domains, allowing for distinct cold-sensitive properties among vertebrate TRPM8 orthologs. The acquisition of cold sensitivity in TRPM8 facilitated terrestrial adaptation during the water-to-land transition.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Medicinal
Sho Sanechika, Chika Shimobori, Katsuya Ohbuchi
Summary: This study evaluated the effects of flavonoid aglycones and their glycosides on TRP channel subtypes, finding that certain compounds can activate TRPA1 and inhibit TRPM8. The metabolization of these compounds in the intestines could potentially lead to the generation of TRPA1 agonists and TRPM8 antagonists.
JOURNAL OF NATURAL MEDICINES
(2021)
Article
Endocrinology & Metabolism
Alex L. Fedinec, Jianxiong Liu, Rong Zhang, Mimily Harsono, Massroor Pourcyrous, Helena Parfenova
Summary: Mild hypothermia produced by head cooling acutely and reversibly reduces specific endothelium-dependent cerebral vasodilator functions via TRPM8 activation, while preserving cerebral arteriolar smooth muscle functions.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2021)
Article
Health Care Sciences & Services
Denis E. Naumov, Olesya O. Kotova, Dina A. Gassan, Ivana Y. Sugaylo, Evgeniya Y. Afanas'eva, Elizaveta G. Sheludko, Juliy M. Perelman
Summary: The study found that TRPM8 and TRPA1 gene polymorphisms do not significantly influence susceptibility to COPD, but the TRPA1 rs7819749 and TRPM8 rs11562975 genotypes are significantly associated with airway obstruction and lung function in COPD patients.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Weifeng Tian, Dongmei He, Jingjing Liu, Feiyu Chen, Wenjie Zhang, Jinsheng Hu, Shu Wang
Summary: Borneol, a traditional Chinese medicine, has been used to treat itchy skin. This study shows that applying borneol topically on the skin can effectively suppress drug-induced itching. Borneol's antipruritic effect is mainly achieved through inhibiting TRPA1 channels and activating TRPM8 channels.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Yushun Tian, Ismael Morin-Poulard, Xiaohui Liu, Nathalie Vanzo, Michele Crozatier
Summary: Hematopoietic stem and progenitor cells maintain blood cell homeostasis by integrating mechanical cues from the vascular niche. Mechanical forces are transduced by the Piezo channel, leading to activation of Notch pathway and repression of FGF signaling, which regulate hematopoietic progenitor maintenance.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Neurosciences
Somayeh Ezzatpanah, Mina Baarnes Eriksen, Anne-Mari Gjestvang Moe, Fred Haugen
Summary: Chronic exposure to cold temperatures can alter thermosensory behaviors in mice, making them appear less sensitive to cold, potentially involving the TRPM8 molecular cold sensor in the process.
Review
Biochemistry & Molecular Biology
Anna-Sophie Mursch-Edlmayr, Matthias Bolz, Clemens Strohmaier
Summary: Glaucomatous optic neuropathies have long been considered diseases caused by high intraocular pressure, but recent research has shown that vascular factors also play an important role in glaucoma. Advances in the study of ocular vasculature, ciliary circulation, retinal circulation, etc., may bring new breakthroughs in the treatment of glaucoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Amie J. Moyes, Sandy M. Chu, Aisah A. Aubdool, Matthew S. Dukinfield, Kenneth B. Margulies, Kenneth C. Bedi, Kairbaan Hodivala-Dilke, Reshma S. Baliga, Adrian J. Hobbs
EUROPEAN HEART JOURNAL
(2020)
Article
Peripheral Vascular Disease
Fulye Argunhan, Dibesh Thapa, Aisah Aniisah Aubdool, Emanuele Carlini, Kate Arkless, Erica Ruth Hendrikse, Joao de Sousa Valente, Xenia Kodji, Brentton Barrett, Carlo Alberto Ricciardi, Luigi Gnudi, Debbie Lucy Hay, Susan Diana Brain
Summary: CGRP plays a protective role in cardiovascular function by restoring blood pressure and preventing cardiovascular remodeling when nitric oxide production is diminished. Endogenous CGRP acts to protect cardiovascular function, while exogenous CGRP can rescue affected mice.
Article
Biochemistry & Molecular Biology
Joao de Sousa Valente, Khadija M. Alawi, Sabah Bharde, Ali A. Zarban, Xenia Kodji, Dibesh Thapa, Fulye Argunhan, Brentton Barrett, Istvan Nagy, Susan D. Brain
Summary: The study found that Englerin A has anti-inflammatory and analgesic effects via the TRPC4/5 pathway in the carrageenan model. It inhibited thermal hyperalgesia and mechanical allodynia, reduced paw oedema, and induced cobalt uptake in DRG neurons. Furthermore, Englerin A showed TRPC4/5-independent effects in vivo, indicating further research is needed to understand its mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Jianyang Hu, Yuni Lai, Hao Huang, Saravanan Ramakrishnan, Yilin Pan, Victor W. S. Ma, Wah Cheuk, Grace Y. K. So, Qingling He, C. Geoffrey Lau, Liang Zhang, William C. S. Cho, Kui Ming Chan, Xin Wang, Y. Rebecca Chin
Summary: In TNBC, TCOF1 is frequently upregulated and its high expression is associated with poor prognosis. Depletion of TCOF1 significantly inhibits the growth and stemness of basal-like TNBC, but not of mesenchymal-like cells, highlighting molecular dependency differences among different TNBC subgroups. Furthermore, TCOF1 mediates TNBC stemness by regulating multiple stem cell molecules.
BRITISH JOURNAL OF CANCER
(2022)
Article
Biology
Dibesh Thapa, Joao de Sousa Valente, Brentton Barrett, Matthew John Smith, Fulye Argunhan, Sheng Y. Lee, Sofya Nikitochkina, Xenia Kodji, Susan D. Brain
Summary: Ageing is associated with increased vulnerability to environmental cold exposure, leading to impaired behavioural and vascular responses to skin local environmental cooling in aged mice. The decline in TRPM8 gene/protein expression in ageing mice suggests a reliance on remaining TRP receptor activity for cold-induced vascular response. Sympathetic-induced vasoconstriction is also reduced with aging, indicating a decline in the cold-induced vascular response necessary for maintaining body heat and health.
Article
Chemistry, Medicinal
Xenia Kodji, Zizheng Kee, Robyn McKenna, Joao de Sousa Valente, Harriet Ravenscroft, Hayley McMillan, John Gamble, Yvonne Dombrowski, Paul Moynagh, David Brough, Fionnuala T. Lundy, Susan D. Brain, Ikhlas A. El Karim
Summary: Stimulation of TRPA1 plays a key role in TMJ pain, while inhibition of the inflammasome also attenuates pain-like behaviors and inflammatory markers. This suggests that NLRP3 inhibition may offer a new therapeutic strategy for TMJ inflammation and pain, in addition to targeting TRPA1.
Article
Chemistry, Medicinal
Dibesh Thapa, Brentton Barrett, Fulye Argunhan, Susan D. Brain
Summary: This study investigated the role of TRPA1 and TRPM8 in thermoregulatory behavioral responses to environmental cold exposure. The researchers successfully avoided physical restraint of mice by administering TRP antagonists orally with palatable food. The results showed that TRPM8 played a direct role in the thermoregulation response to whole body cold exposure in mice, and increased TRPM8 levels after cold exposure could be a protective response.
Article
Multidisciplinary Sciences
Cristina Perez-Ternero, Aisah A. Aubdool, Raj Makwana, Gareth J. Sanger, Roland H. Stimson, Li F. Chan, Amie J. Moyes, Adrian J. Hobbs
Summary: C-type natriuretic peptide (CNP) plays a crucial role in lipid homeostasis and energy balance by regulating the expression of uncoupling protein-1 and lipid utilization. CNP exerts these metabolic regulatory actions by inhibiting sympathetic thermogenic programming and reducing peroxisome proliferator-activated receptor-gamma coactivator-1 alpha expression, while driving adipogenesis via NPR-B/protein kinase-G.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Physiology
Fulye Argunhan, Susan D. Brain
Summary: The treatment of hypertension and heart failure is a major challenge, with over 26 million people affected worldwide. CGRP has been found to have beneficial effects in hypertensive and heart failure patients. However, due to its short half-life, the beneficial effects of CGRP are often acute and transient. Recently developed long-lasting CGRP agonists may provide a solution for further studying CGRP in cardiovascular disease.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Microbiology
Liziane Cristine Malaquias da Silva, Fernanda da Silva Platner, Lauany da Silva Fonseca, Virgilio Frota Rossato, Dian Carlos Pereira de Andrade, Joao de Sousa Valente, Susan Diana Brain, Elizabeth Soares Fernandes
Summary: This study conducted a systematic review to determine the most frequent ocular manifestations of Chikungunya virus (CHIKV) infection and their associations with gender and age. The most common ocular symptoms of CHIKV infection were ocular pain, inflammation, and reduced visual acuity, while conjunctivitis and optic neuritis were the most common manifestations. These occurred mostly in women with an average age of 42 years.
Article
Biochemistry & Molecular Biology
Ali A. Zarban, Hiba Chaudhry, Joao de Sousa Valente, Fulye Argunhan, Hala Ghanim, Susan D. Brain
Summary: This study investigates the role of CGRP in regulating oedema formation and neutrophil accumulation in the cutaneous microvasculature of mice. The results suggest that exogenous CGRP enhances oedema formation induced by substance P and TNF alpha, and sensory nerves play a role in mediating this response. Furthermore, endogenous CGRP contributes to the inflammatory response in skin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Xinmei Huang, Shenghua Zhang, Jingshu Tang, Tian Tian, Yilin Pan, Lina Wu, Jingxuan Zhang, Yang Liu, Jing Huang, Hui Dai, Weiyan Xu, Youhui Zhang, Jinfeng Chen, Mengshu Cao, Liang Zhang, Xiaoyan Qiu
Summary: Elevated expression of IgG in cancer cells is associated with increased malignancy and poor prognosis. A nonconventional sialylation modification is critical for the function of cancer-derived IgG, and OCT4 and SOX2 are identified as key factors that promote its expression. The SIA-cIgG/c-Met/SOX2/SIA-cIgG signaling loop is highly active in stem-like cells from epithelial cancers, driving cancer stemness. Targeting SIA-cIgG with the mAb RP215 effectively blocks this signaling loop and inhibits lung cancer cell stemness and tumor growth.
Article
Biochemical Research Methods
Yilin Pan, Christine Yim-Ping Wong, Haiying Ma, Ryan Tsz-Hei Tse, Carol Ka-Lo Cheng, Miaomiao Tan, Peter Ka-Fung Chiu, Jeremy Yuen-Chun Teoh, Xin Wang, Chi-Fai Ng, Liang Zhang
Summary: Urine proteome is a valuable source of biomarkers for disease diagnosis and monitoring. This study compared the proteome composition of renal pelvis urine (RPU) and self-voided bladder urine (BU) samples from male patients with unilateral urinary tract obstruction, and revealed no significant differences at the protein level between the two urine types. However, there were more overrepresented proteins in BU samples, and the BU samples showed an increase in collagen peptides with hydroxyproline modifications.
PROTEOMICS CLINICAL APPLICATIONS
(2023)