Article
Biophysics
Hui Dong, Le Zhao, Xu Zhu, Xiuhua Wei, Menghui Zhu, Qingmin Ji, Xueke Luo, Yintang Zhang, Yanli Zhou, Maotian Xu
Summary: In this study, a turn-on ratiometric electrochemical sensor was fabricated for the detection of beta-Galactosidase. The sensor showed rapid, simple, accurate, and highly sensitive detection with a wide linear range and low detection limit.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Jungwook Kwon, Ho Young Kang, Haesik Yang
Summary: A new electrochemical method for detecting E. coli was developed in this study, which does not require a permeabilization process. The presence of MPGP during induction of Gal expression led to increased production of MP. The detection limit of this method in both drinking water and tap water was similar to 2 x 10(3) CFU/mL.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Shijun Chen, Lin Wang, Xiaodong Ma, Yuanyuan Wu, Shicong Hou
Summary: A novel near-infrared proportional fluorescent probe was designed and screened to detect the activity of beta-galactosidase in cancer and senescent cells. The probe showed a rapid response, large stoke-shift, and significant fluorescence enhancement after interaction with beta-galactosidase. It has good biocompatibility and can effectively avoid interference from biological background. The probe was successfully used to detect and image endogenous beta-galactosidase in cells and zebrafish, and evaluate the therapeutic effects of drug combination on senescent cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Yana Chen, Shuangling Wang, Jujie Ren, Haiyan Zhao, Min Cui, Na Li, Meng Li, Cong Zhang
Summary: In this study, a signal on-off ratiometric electrochemical immunosensor was developed for ultrasensitive detection of A beta oligomer, which serves as a reliable biomarker for early diagnosis of Alzheimer's disease. The sandwich-like electrochemical immunosensor exhibited high sensitivity and low detection limit, and was able to determine the content of A beta oligomer in real cerebrospinal fluid samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Chang Yin, Yong Wang, Qianfen Zhuang
Summary: A dual-ratiometric electrochemical sensor was developed for propyl gallate (PG) detection using anthraquinone/multi-walled carbon nanotube/chitosan modified glassy carbon electrode (AQ/MWCNT/CS/GCE), which demonstrated enhanced sensitivity for PG detection. The sensor showed linear increase in two ratiometric signals, IPG/IAQ and IPG/IMWCNT, with increasing PG concentration, and achieved detection limits of 0.67 µM and 0.76 µM, respectively. The sensor was successfully applied for PG detection in edible oil samples.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Qiong Hu, Jianwen Wan, Haocheng Wang, Xiaojing Cao, Shiqi Li, Yiyi Liang, Yilin Luo, Wei Wang, Li Niu
Summary: A boronate-affinity cross-linking method is demonstrated for the highly sensitive and selective detection of glycoconjugates. The method utilizes a methylene blue-tagged nucleic acid aptamer to selectively capture target glycoconjugates, followed by labeling a large number of ferrocene tags through boronate-affinity cross-linking. The dual-signal output from the methylene blue and ferrocene tags enables ratiometric detection. This method is simple, cost-effective, and possesses high robustness and reproducibility.
ANALYTICAL CHEMISTRY
(2022)
Article
Spectroscopy
Shijun Chen, Mengyao Liu, Yunjiang Zi, Junyi He, Lin Wang, Yuanyuan Wu, Shicong Hou, Wenxue Wu
Summary: Beta-galactosidase (beta-gal) is a hydrolytic enzyme in lysosomes and an important biomarker of cellular senescence and ovarian cancer. We designed an enzyme-activated near-infrared probe for real-time detection of beta-gal activity in vivo. The probe has short response time, large Stokes displacement, and near-infrared fluorescence emission, and has been successfully used to detect beta-gal in cancer cells and senile cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Chengxi Zhu, Xi Wang, Dongmei Yu, Lixing Chen, Xiaoxin Han
Summary: We report a hairpin DNA-enabled ratiometric electrochemical aptasensor for sensitive and reliable detection of malathion (MAL). The approach utilizes hairpin DNA as a carrier to form double-stranded DNA structures on an electrode. The presence of MAL induces the removal of aptamers, causing a decrease in the oxidation current of MB and an increase in the oxidation current of Fc. The ratiometric signal of I-Fc/I-MB responds quantitatively to MAL concentrations.
Article
Chemistry, Analytical
Ali Khodadoust, Navid Nasirizadeh, Ramezan Ali Taheri, Mohammad Dehghani, Mostafa Ghanei, Hasan Bagheri
Summary: A sensitive biosensor has been developed for the detection of miR-141, which shows potential for early diagnosis of non-small cell lung cancer. The biosensor exhibited a low detection limit and a wide linear range, indicating its effectiveness in clinical applications.
Article
Chemistry, Analytical
Min Chen, Rui Han, Yang Li, Xiliang Luo
Summary: An efficient strategy for the establishment of ratiometric antifouling electrochemical biosensors was proposed, utilizing designed functional peptides and synthesized graphene oxide-nile blue A (GO-NB) nanocomposites. The biosensor exhibited a linear range from 5.0 pg mL(-1) to 100 ng mL(-1) and a limit of detection of 1.26 pg mL(-1), providing stability and accuracy in complex biological systems.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Sakthivel Kumaravel, Sheng-En Jian, Sheng-Tung Huang, Chih-Hung Huang, Wei-Zhe Hong
Summary: Developing an easy-to-operate Salmonella-targeting point-of-care detection platform is urgently needed for safeguarding public health and the economy. Researchers designed two activity-based latent ratiometric electrochemical molecular substrates for specific and rapid profiling of Salmonella esterase, enabling easy and selective detection of live Salmonella.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Yujie Sun, Qi Dong, Huan Yang, Weiling Song, Hong Zhou
Summary: In this study, a ratiometric electrochemical biosensor based on CuS quantum dots (CuS QDs) regulated by DNAzyme was developed for telomerase detection. Telomerase activity was measured by extending the substrate probe with repeated sequence, releasing CuS QDs as an input to the DNAzyme-modified electrode. The detection range was 1.0 x 10-12 - 1.0 x 10-6 IU/L, with a limit of detection of 2.75 x 10-14 IU/L. Telomerase activity in HeLa extracts was also tested to demonstrate its clinical application.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Fatima Mustafa, Steve Liebich, Silvana Andreescu
Summary: A novel amplification approach using multivalent cerium oxide nanoparticles (CeNPs) is proposed to enhance the sensitivity of colorimetric assays for detection of beta-galactosidase (beta-Gal) activity. The method catalyzes the oxidation of 4-aminophenol (4-AP) produced in the hydrolysis process of the substrate, resulting in increased detection sensitivity in the visible range. The assay shows high sensitivity and ease of use, with potential broad applications in various fields.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biophysics
Kanjana Kunpatee, Kannika Khantasup, Kittinan Komolpis, Abdulhadee Yakoh, Suphachai Nuanualsuwan, Mohini M. Sain, Sudkate Chaiyo
Summary: This study developed an electrochemical lateral flow immunoassay (eLFIA) strip with high reproducibility for rapid and accurate detection of Streptococcus suis serotype 2. The strip integrated ratiometric electrochemical detection and LFIA, resulting in improved reproducibility compared to a single electrode. The biosensor showed good linearity and a low limit of detection, and was successfully applied in human serum samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Shiyi Xie, Cong Zhu, Lijuan Yang, Huiyi Li, Haizhen Zhu, Zhou Nie, Chunyang Lei
Summary: This study presents a ratiometric electrochemical sensor that detects viral proteases with high sensitivity by integrating target proteolysis-activated in vitro transcription and a DNA-functionalized electrochemical interface. The sensor demonstrated robust and specific NS3/4A protease sensing with sub-femtomolar sensitivity, and showed potential for developing direct-acting antivirals and novel therapies for viral infections.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Organic
Sean Goggins, Ellen A. Apsey, Mary F. Mahon, Christopher G. Frost
ORGANIC & BIOMOLECULAR CHEMISTRY
(2017)
Article
Chemistry, Multidisciplinary
Sean Goggins, Oliver P. Stark, Christophe Naz, Barrie J. Marsh, Christopher G. Frost
SUPRAMOLECULAR CHEMISTRY
(2017)
Article
Computer Science, Information Systems
Joshua Introne, Ingrid Erickson, Bryan Semaan, Sean Goggins
JOURNAL OF THE ASSOCIATION FOR INFORMATION SCIENCE AND TECHNOLOGY
(2020)
Article
Chemistry, Multidisciplinary
James A. R. Tilden, Anneke T. Lubben, Shaun B. Reeksting, Gabriele Kociok-Kohn, Christopher G. Frost
Summary: Selective bioconjugation remains a significant challenge for synthetic chemists due to the high degree of functionality and stringent reaction conditions of biomolecules. This study reports the preparation of Pd(II) complexes for cysteine bioconjugation via a facile C-H activation process. These complexes demonstrate competitive bioconjugation efficiency and are synthesized using user-friendly methods and common Pd(II) sources, making them more cost-effective. Furthermore, these complexes do not require isolation and achieve high conversion efficiency and selectivity of a model peptide. They also show the ability to selectively arylate a single surface cysteine residue on a model protein substrate, further highlighting their utility.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Organic
Sam A. Spring, Sean Goggins, Christopher G. Frost
Summary: A dual pseudo-enantiomer system was developed, where two asymmetric catalytic cycles could be selectively triggered to afford an enantioenriched product. The system showed high enantioselectivity in the reduction of prochiral ketone following external stimulation.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)
Proceedings Paper
Computer Science, Theory & Methods
Kevin Lumbard, Anna Buhman, Gabrielle Wethor, Matthew Hale, Sean Goggins, Matt Germonprez
AMCIS 2020 PROCEEDINGS
(2020)
Article
Education & Educational Research
Marcela Borge, Yann Shiou Ong, Sean Goggins
ETR&D-EDUCATIONAL TECHNOLOGY RESEARCH AND DEVELOPMENT
(2020)
Article
Psychology, Multidisciplinary
Joshua Introne, Sean Goggins
COMPUTERS IN HUMAN BEHAVIOR
(2019)
Article
Chemistry, Analytical
Sam A. Spring, Sean Goggins, Christopher G. Frost
Article
Chemistry, Multidisciplinary
Jamie A. Leitch, Callum J. Heron, Janette McKnight, Gabriele Kociok-Kohn, Yunas Bhonoah, Christopher G. Frost
CHEMICAL COMMUNICATIONS
(2017)
Review
Chemistry, Multidisciplinary
Jamie A. Leitch, Christopher G. Frost
CHEMICAL SOCIETY REVIEWS
(2017)
Article
Chemistry, Multidisciplinary
Jamie A. Leitch, Claire L. McMullin, Andrew J. Paterson, Mary F. Mahon, Yunas Bhonoah, Christopher G. Frost
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2017)
Article
Psychology, Multidisciplinary
Wanli Xing, Sean Goggins, Josh Introne
COMPUTERS IN HUMAN BEHAVIOR
(2018)
Article
Chemistry, Organic
Faiza Diaba, Gisela Trenchs
Summary: The first violet light-mediated synthesis of gamma- and delta-lactams from N-alkenyl trichloroacetamides is reported in this paper. The reactions are conducted in tetrahydrofuran or 2-methyltetrahydrofuran as the sole solvent without catalysts or additives, under non-anhydrous conditions in an air atmosphere where the solvent serves as the radical initiator.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Feroze Hussain, Sajjad Ahmed, Ashiq Hussain Padder, Qazi Naveed Ahmed
Summary: This study reports a novel and efficient one-pot synthesis method for mixed phosphorotrithioates, which does not require supplementary additives and shows broad applicability.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Hyunjin Oh, Ikyon Kim
Summary: A new 1,2,4-triazole-pyrrolo[1,2-a]pyrazine hybrid system, 6-acylpyrrolo[1,2-a][1,2,4]triazolo[5,1-c]pyrazine, was synthesized using a catalyst-free method. This method involved sequential exposure of pyrrole-2-carbonitrile-derived substrates to DMF-DMA and acyl hydrazide, resulting in the formation of acylated pyrazine and 1,2,4-triazole rings, enabling the installation of various substituents at specific positions on the core skeleton.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Ming Yan, Si-fan Wang, Yong-po Zhang, Jin-zhong Zhao, Zhuo Tang, Guang-xun Li
Summary: Here we developed an efficient photocatalytic approach for the convenient preparation of sulfinamides. The reaction allows for the gram-scale preparation of sulfinamides and the one-pot synthesis of various sulfonyl amides.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Farzaneh Bandehali-Naeini, Zahra Tanbakouchian, Noushin Farajinia-Lehi, Nicolas Mayer, Morteza Shiri, Martin Breugst
Summary: Two tandem catalytic systems were developed for the synthesis of novel 3,4-disubstituted maleimides using the same Ugi adducts. Different maleimide structures can be synthesized using either Pd or Cu catalysis.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Tanya Raghava, Anjan Chattopadhyay, Subhadeep Banerjee, Nivedita Sarkar
Summary: Amine substitution of two ortho fluorine atoms of tetrafluoroterephthalonitrile through SNAr chemistry is easily achievable. But further fluorine substitution is only possible under forcing conditions, yielding valuable fluorophores for bioimaging.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Review
Chemistry, Organic
Anju Chadha, Santosh Kumar Padhi, Selvaraj Stella, Sowmyalakshmi Venkataraman, Thangavelu Saravanan
Summary: Alcohol dehydrogenases are enzymes that use cofactors for oxidation or reduction reactions of alcohols or carbonyl compounds. They are utilized in green chemistry and have applications in the production of pharmaceuticals. Recombinant enzymes have solved the challenge of producing purified enzymes in large quantities. Engineered alcohol dehydrogenases have been used in asymmetric synthesis in industry. Various methods have been established for regenerating expensive cofactors to make the enzymatic process more efficient and economically viable.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
